Practical considerations when using temperature to obtain rate constants and activation thermodynamics of enzymes with two catalytic steps: native and N460T-beta-galactosidase (E. coli) as examples.

LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance. Protein Sci (2012) 1.19

Systems analysis of cancer cell heterogeneity in caspase-dependent apoptosis subsequent to mitochondrial outer membrane permeabilization. J Biol Chem (2012) 0.80

Equilibria and stability of a class of positive feedback loops. J Math Biol (2013) 0.78

Role of Met-542 as a guide for the conformational changes of Phe-601 that occur during the reaction of β-galactosidase (Escherichia coli). Biochem Cell Biol (2010) 1.23

LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance. Protein Sci (2012) 1.19

Gata3 participates in a complex transcriptional feedback network to regulate sympathoadrenal differentiation. Development (2006) 1.15

Physical and kinetic properties of the family 3 beta-glucosidase from Aspergillus niger which is important for cellulose breakdown. Protein J (2004) 1.00

Direct and indirect roles of His-418 in metal binding and in the activity of beta-galactosidase (E. coli). Protein Sci (2009) 0.93

Elucidating factors important for monovalent cation selectivity in enzymes: E. coli β-galactosidase as a model. Phys Chem Chem Phys (2015) 0.89

A study of the relationships of interactions between Asp-201, Na+ or K+, and galactosyl C6 hydroxyl and their effects on binding and reactivity of beta-galactosidase. Biochem Cell Biol (2004) 0.89

Ser-796 of β-galactosidase (Escherichia coli) plays a key role in maintaining a balance between the opened and closed conformations of the catalytically important active site loop. Arch Biochem Biophys (2011) 0.83

Substitution for Asn460 cripples β-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability. Arch Biochem Biophys (2012) 0.82

Transglucosidic reactions of the Aspergillus niger family 3 beta-glucosidase: qualitative and quantitative analyses and evidence that the transglucosidic rate is independent of pH. Arch Biochem Biophys (2005) 0.82

Importance of Arg-599 of β-galactosidase (Escherichia coli) as an anchor for the open conformations of Phe-601 and the active-site loop. Biochem Cell Biol (2010) 0.82

Studies of Glu-416 variants of beta-galactosidase (E. coli) show that the active site Mg(2+) is not important for structure and indicate that the main role of Mg (2+) is to mediate optimization of active site chemistry. Protein J (2010) 0.81

Structural basis for the altered activity of Gly794 variants of Escherichia coli beta-galactosidase. Biochemistry (2003) 0.80

Structural explanation for allolactose (lac operon inducer) synthesis by lacZ β-galactosidase and the evolutionary relationship between allolactose synthesis and the lac repressor. J Biol Chem (2013) 0.79

Trp-49 of the family 3 beta-glucosidase from Aspergillus niger is important for its transglucosidic activity: creation of novel beta-glucosidases with low transglucosidic efficiencies. Arch Biochem Biophys (2006) 0.78

Beta-galactosidase (Escherichia coli) has a second catalytically important Mg2+ site. Biochem Biophys Res Commun (2006) 0.78

Beta-galactosidases (Escherichia coli) with double substitutions show that Tyr-503 acts independently of Glu-461 but cooperatively with Glu-537. J Protein Chem (2003) 0.77

Trp-262 is a key residue for the hydrolytic and transglucosidic reactivity of the Aspergillus niger family 3 beta-glucosidase: substitution results in enzymes with mainly transglucosidic activity. Arch Biochem Biophys (2005) 0.76

Beta-D-galactopyranosyl azide: its one-step quantitative synthesis using E461G-beta-galactosidase (Escherichia coli) and a demonstration of its potential as a reagent for molecular biology. Appl Biochem Biotechnol (2003) 0.75