Role of Met-542 as a guide for the conformational changes of Phe-601 that occur during the reaction of β-galactosidase (Escherichia coli).

RELION: implementation of a Bayesian approach to cryo-EM structure determination. J Struct Biol (2012) 10.13

High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy. Ultramicroscopy (2013) 3.24

Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopy. J Mol Biol (2011) 3.16

Semi-automated selection of cryo-EM particles in RELION-1.3. J Struct Biol (2014) 1.68

LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance. Protein Sci (2012) 1.19

Molecular mechanism of antibody-mediated activation of β-galactosidase. Structure (2014) 1.10

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2. Elife (2016) 1.04

Problems in obtaining perfect images by single-particle electron cryomicroscopy of biological structures in amorphous ice. Microscopy (Oxf) (2013) 1.04

Structural explanation for allolactose (lac operon inducer) synthesis by lacZ β-galactosidase and the evolutionary relationship between allolactose synthesis and the lac repressor. J Biol Chem (2013) 0.79

Picomolar inhibition of β-galactosidase (bovine liver) attributed to loop closure. Bioorg Med Chem (2017) 0.75

Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis. PLoS Pathog (2009) 2.16

Characterization of 3-ketosteroid 9{alpha}-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis. J Biol Chem (2009) 1.64

Structure of human NMN adenylyltransferase. A key nuclear enzyme for NAD homeostasis. J Biol Chem (2001) 1.23

LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance. Protein Sci (2012) 1.19

Gata3 participates in a complex transcriptional feedback network to regulate sympathoadrenal differentiation. Development (2006) 1.15

A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism. J Biol Chem (2010) 1.04

Physical and kinetic properties of the family 3 beta-glucosidase from Aspergillus niger which is important for cellulose breakdown. Protein J (2004) 1.00

Improving biophysical properties of a bispecific antibody scaffold to aid developability: quality by molecular design. MAbs (2013) 0.98

The sec14 homology module of neurofibromin binds cellular glycerophospholipids: mass spectrometry and structure of a lipid complex. J Mol Biol (2006) 0.97

Circular permutation of Bacillus circulans xylanase: a kinetic and structural study. Biochemistry (2010) 0.95

Direct and indirect roles of His-418 in metal binding and in the activity of beta-galactosidase (E. coli). Protein Sci (2009) 0.93

Elucidating factors important for monovalent cation selectivity in enzymes: E. coli β-galactosidase as a model. Phys Chem Chem Phys (2015) 0.89

A study of the relationships of interactions between Asp-201, Na+ or K+, and galactosyl C6 hydroxyl and their effects on binding and reactivity of beta-galactosidase. Biochem Cell Biol (2004) 0.89

The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension. J Mol Biol (2004) 0.86

Expression, purification and preliminary crystallographic characterization of a novel segment from the neurofibromatosis type 1 protein. Acta Crystallogr D Biol Crystallogr (2004) 0.84

Structural diversity of the active N-terminal kinase domain of p90 ribosomal S6 kinase 2. PLoS One (2009) 0.84

Ser-796 of β-galactosidase (Escherichia coli) plays a key role in maintaining a balance between the opened and closed conformations of the catalytically important active site loop. Arch Biochem Biophys (2011) 0.83

Transglucosidic reactions of the Aspergillus niger family 3 beta-glucosidase: qualitative and quantitative analyses and evidence that the transglucosidic rate is independent of pH. Arch Biochem Biophys (2005) 0.82

Substitution for Asn460 cripples β-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability. Arch Biochem Biophys (2012) 0.82

Importance of Arg-599 of β-galactosidase (Escherichia coli) as an anchor for the open conformations of Phe-601 and the active-site loop. Biochem Cell Biol (2010) 0.82

Studies of Glu-416 variants of beta-galactosidase (E. coli) show that the active site Mg(2+) is not important for structure and indicate that the main role of Mg (2+) is to mediate optimization of active site chemistry. Protein J (2010) 0.81

Strategies for modulating the pH-dependent activity of a family 11 glycoside hydrolase. Biochemistry (2013) 0.81

Metal requirements and phosphodiesterase activity of tRNase Z enzymes. Biochemistry (2007) 0.80

Structural basis for the altered activity of Gly794 variants of Escherichia coli beta-galactosidase. Biochemistry (2003) 0.80

Structural explanation for allolactose (lac operon inducer) synthesis by lacZ β-galactosidase and the evolutionary relationship between allolactose synthesis and the lac repressor. J Biol Chem (2013) 0.79

Structural and biochemical consequences of NF1 associated nontruncating mutations in the Sec14-PH module of neurofibromin. Hum Mutat (2011) 0.79

Beta-galactosidase (Escherichia coli) has a second catalytically important Mg2+ site. Biochem Biophys Res Commun (2006) 0.78

The crystal structure of the active form of the C-terminal kinase domain of mitogen- and stress-activated protein kinase 1. J Mol Biol (2010) 0.78

Trp-49 of the family 3 beta-glucosidase from Aspergillus niger is important for its transglucosidic activity: creation of novel beta-glucosidases with low transglucosidic efficiencies. Arch Biochem Biophys (2006) 0.78

Beta-galactosidases (Escherichia coli) with double substitutions show that Tyr-503 acts independently of Glu-461 but cooperatively with Glu-537. J Protein Chem (2003) 0.77

Practical considerations when using temperature to obtain rate constants and activation thermodynamics of enzymes with two catalytic steps: native and N460T-beta-galactosidase (E. coli) as examples. Protein J (2009) 0.77

Trp-262 is a key residue for the hydrolytic and transglucosidic reactivity of the Aspergillus niger family 3 beta-glucosidase: substitution results in enzymes with mainly transglucosidic activity. Arch Biochem Biophys (2005) 0.76

Beta-D-galactopyranosyl azide: its one-step quantitative synthesis using E461G-beta-galactosidase (Escherichia coli) and a demonstration of its potential as a reagent for molecular biology. Appl Biochem Biotechnol (2003) 0.75