Published in Proteins on August 01, 2009
Improving the thermostability and catalytic efficiency of Bacillus deramificans pullulanase by site-directed mutagenesis. Appl Environ Microbiol (2013) 0.95
Structure and mutagenesis of the DNA modification-dependent restriction endonuclease AspBHI. Sci Rep (2014) 0.86
Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii. MBio (2015) 0.84
Enhancing the secretion efficiency and thermostability of a Bacillus deramificans pullulanase mutant (D437H/D503Y) by N-terminal domain truncation. Appl Environ Microbiol (2015) 0.81
Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli. Microb Cell Fact (2016) 0.81
Truncation of the unique N-terminal domain improved the thermos-stability and specific activity of alkaline α-amylase Amy703. Sci Rep (2016) 0.78
Soluble expression of pullulanase from Bacillus acidopullulyticus in Escherichia coli by tightly controlling basal expression. J Ind Microbiol Biotechnol (2014) 0.78
The Sus operon: a model system for starch uptake by the human gut Bacteroidetes. Cell Mol Life Sci (2016) 0.77
Structure of the starch-debranching enzyme barley limit dextrinase reveals homology of the N-terminal domain to CBM21. Acta Crystallogr Sect F Struct Biol Cryst Commun (2012) 0.76
Active Hydrogen Bond Network (AHBN) and Applications for Improvement of Thermal Stability and pH-Sensitivity of Pullulanase from Bacillus naganoensis. PLoS One (2017) 0.75
Disorder prediction-based construct optimization improves activity and catalytic efficiency of Bacillus naganoensis pullulanase. Sci Rep (2016) 0.75
Overview of the CCP4 suite and current developments. Acta Crystallogr D Biol Crystallogr (2011) 67.55
Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem J (2004) 8.19
An evolving hierarchical family classification for glycosyltransferases. J Mol Biol (2003) 6.48
Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components. Proc Natl Acad Sci U S A (2011) 3.59
A potent mechanism-inspired O-GlcNAcase inhibitor that blocks phosphorylation of tau in vivo. Nat Chem Biol (2008) 3.46
Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA. Chem Biol (2002) 2.30
Dimeric dUTPases, HisE, and MazG belong to a new superfamily of all-alpha NTP pyrophosphohydrolases with potential "house-cleaning" functions. J Mol Biol (2005) 2.14
Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification. EMBO J (2006) 1.98
Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex. Proc Natl Acad Sci U S A (2003) 1.92
Mechanistic insights into glycosidase chemistry. Curr Opin Chem Biol (2008) 1.85
Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold. Nat Struct Biol (2002) 1.82
Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently. Nat Biotechnol (2002) 1.81
House cleaning, a part of good housekeeping. Mol Microbiol (2006) 1.80
Structural and biochemical evidence for a boat-like transition state in beta-mannosidases. Nat Chem Biol (2008) 1.75
Structure of an O-GlcNAc transferase homolog provides insight into intracellular glycosylation. Nat Struct Mol Biol (2008) 1.66
Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27. Structure (2003) 1.63
The crystal structure of Trypanosoma cruzi dUTPase reveals a novel dUTP/dUDP binding fold. Structure (2004) 1.59
The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions. PLoS Biol (2009) 1.57
Directed evolution of enzyme stability. Biomol Eng (2005) 1.56
Characterization and engineering of the bifunctional N- and O-glucosyltransferase involved in xenobiotic metabolism in plants. Proc Natl Acad Sci U S A (2007) 1.55
Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity. Nat Struct Mol Biol (2006) 1.52
Recent insights into copper-containing lytic polysaccharide mono-oxygenases. Curr Opin Struct Biol (2013) 1.50
Analysis of PUGNAc and NAG-thiazoline as transition state analogues for human O-GlcNAcase: mechanistic and structural insights into inhibitor selectivity and transition state poise. J Am Chem Soc (2007) 1.46
Genome analyses highlight the different biological roles of cellulases. Nat Rev Microbiol (2012) 1.46
The Protein Information Management System (PiMS): a generic tool for any structural biology research laboratory. Acta Crystallogr D Biol Crystallogr (2011) 1.45
The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution. J Biol Chem (2003) 1.43
Crystal structure of dihydrodipicolinate synthase (BA3935) from Bacillus anthracis at 1.94 A resolution. Proteins (2006) 1.40
Evidence that family 35 carbohydrate binding modules display conserved specificity but divergent function. Proc Natl Acad Sci U S A (2009) 1.38
Differential oligosaccharide recognition by evolutionarily-related beta-1,4 and beta-1,3 glucan-binding modules. J Mol Biol (2002) 1.36
Evidence for a dual binding mode of dockerin modules to cohesins. Proc Natl Acad Sci U S A (2007) 1.36
Family 6 carbohydrate binding modules in beta-agarases display exquisite selectivity for the non-reducing termini of agarose chains. J Biol Chem (2006) 1.35
The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity. Proc Natl Acad Sci U S A (2007) 1.34
Tailored catalysts for plant cell-wall degradation: redesigning the exo/endo preference of Cellvibrio japonicus arabinanase 43A. Proc Natl Acad Sci U S A (2005) 1.30
Characterization and three-dimensional structures of two distinct bacterial xyloglucanases from families GH5 and GH12. J Biol Chem (2007) 1.30
Elevation of global O-GlcNAc levels in 3T3-L1 adipocytes by selective inhibition of O-GlcNAcase does not induce insulin resistance. J Biol Chem (2008) 1.28
Structural and biochemical analysis of Cellvibrio japonicus xylanase 10C: how variation in substrate-binding cleft influences the catalytic profile of family GH-10 xylanases. J Biol Chem (2003) 1.27
Understanding how diverse beta-mannanases recognize heterogeneous substrates. Biochemistry (2009) 1.27
Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4. Chem Biol (2006) 1.26
The mechanisms by which family 10 glycoside hydrolases bind decorated substrates. J Biol Chem (2003) 1.26
Glycosidase mechanisms. Curr Opin Chem Biol (2002) 1.26
Mechanistic evidence for a front-side, SNi-type reaction in a retaining glycosyltransferase. Nat Chem Biol (2011) 1.24
The copper active site of CBM33 polysaccharide oxygenases. J Am Chem Soc (2013) 1.23
Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF. Proc Natl Acad Sci U S A (2010) 1.21
Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose. Proc Natl Acad Sci U S A (2002) 1.21
Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens. Structure (2003) 1.21
Mechanistic insight into enzymatic glycosyl transfer with retention of configuration through analysis of glycomimetic inhibitors. Angew Chem Int Ed Engl (2010) 1.20
Domain closure, substrate specificity and catalysis of D-lactate dehydrogenase from Lactobacillus bulgaricus. J Mol Biol (2002) 1.18
An ATP-binding cassette-type cysteine transporter in Campylobacter jejuni inferred from the structure of an extracytoplasmic solute receptor protein. Mol Microbiol (2005) 1.16
Structural dissection and high-throughput screening of mannosylglycerate synthase. Nat Struct Mol Biol (2005) 1.16
Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules. Structure (2004) 1.15
Crystal structure of a family GT4 glycosyltransferase from Bacillus anthracis ORF BA1558. Proteins (2008) 1.15
Glycosidase inhibition: assessing mimicry of the transition state. Org Biomol Chem (2009) 1.14
Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state. Angew Chem Int Ed Engl (2002) 1.14
How nature can exploit nonspecific catalytic and carbohydrate binding modules to create enzymatic specificity. Proc Natl Acad Sci U S A (2012) 1.14
Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont. Nat Chem Biol (2009) 1.14
The Cellvibrio japonicus mannanase CjMan26C displays a unique exo-mode of action that is conferred by subtle changes to the distal region of the active site. J Biol Chem (2008) 1.13
How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides: structure and activity of a Clostridium thermocellum lichenase, CtLic26A. J Biol Chem (2005) 1.13
The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A. Structure (2002) 1.12
The crystal structures of human S100A12 in apo form and in complex with zinc: new insights into S100A12 oligomerisation. J Mol Biol (2009) 1.12
Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles. Bioconjug Chem (2006) 1.12
Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation. J Biol Chem (2006) 1.12
Iminosugar glycosidase inhibitors: structural and thermodynamic dissection of the binding of isofagomine and 1-deoxynojirimycin to beta-glucosidases. J Am Chem Soc (2003) 1.11
The three-dimensional structure of the N-acetylglucosamine-6-phosphate deacetylase, NagA, from Bacillus subtilis: a member of the urease superfamily. J Biol Chem (2003) 1.11
Conservation of structure and mechanism in primary and secondary transporters exemplified by SiaP, a sialic acid binding virulence factor from Haemophilus influenzae. J Biol Chem (2006) 1.11
Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis. Structure (2004) 1.11
The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase. EMBO J (2002) 1.09
The Clostridium cellulolyticum dockerin displays a dual binding mode for its cohesin partner. J Biol Chem (2008) 1.06
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus. Biochem J (2005) 1.05
Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? J Biol Chem (2005) 1.05
Convergent evolution sheds light on the anti-beta -elimination mechanism common to family 1 and 10 polysaccharide lyases. Proc Natl Acad Sci U S A (2002) 1.05
MOLE: a data management application based on a protein production data model. Proteins (2005) 1.04
Structure of a group A streptococcal phage-encoded virulence factor reveals a catalytically active triple-stranded beta-helix. Proc Natl Acad Sci U S A (2005) 1.04
Structure/function studies on a S-adenosyl-L-methionine-dependent uroporphyrinogen III C methyltransferase (SUMT), a key regulatory enzyme of tetrapyrrole biosynthesis. J Mol Biol (2004) 1.04
Structure and activity of two metal ion-dependent acetylxylan esterases involved in plant cell wall degradation reveals a close similarity to peptidoglycan deacetylases. J Biol Chem (2006) 1.04
Role of solvation barriers in protein kinetic stability. J Mol Biol (2006) 1.04
Design and synthesis of inhibitors of Plasmodium falciparum N-myristoyltransferase, a promising target for antimalarial drug discovery. J Med Chem (2012) 1.03
Sialic acid mutarotation is catalyzed by the Escherichia coli beta-propeller protein YjhT. J Biol Chem (2007) 1.02
Insights into the molecular determinants of substrate specificity in glycoside hydrolase family 5 revealed by the crystal structure and kinetics of Cellvibrio mixtus mannosidase 5A. J Biol Chem (2004) 1.02
Mannose foraging by Bacteroides thetaiotaomicron: structure and specificity of the beta-mannosidase, BtMan2A. J Biol Chem (2007) 1.02
Structure of components of an intercellular channel complex in sporulating Bacillus subtilis. Proc Natl Acad Sci U S A (2012) 1.02
An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima. J Am Chem Soc (2004) 1.01
Insights into the structural determinants of cohesin-dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA. J Mol Biol (2005) 1.01
Signature active site architectures illuminate the molecular basis for ligand specificity in family 35 carbohydrate binding module. Biochemistry (2010) 1.01
Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification. Biochem J (2011) 1.00
The structure of monoamine oxidase from Aspergillus niger provides a molecular context for improvements in activity obtained by directed evolution. J Mol Biol (2008) 0.99
β-Branched acyclic nucleoside analogues as inhibitors of Plasmodium falciparum dUTPase. Bioorg Med Chem (2011) 0.99
Biosynthesis of the tunicamycin antibiotics proceeds via unique exo-glycal intermediates. Nat Chem (2012) 0.99
The crystal structure of a complex of Campylobacter jejuni dUTPase with substrate analogue sheds light on the mechanism and suggests the "basic module" for dimeric d(C/U)TPases. J Mol Biol (2004) 0.99
Functional analysis of a group A streptococcal glycoside hydrolase Spy1600 from family 84 reveals it is a beta-N-acetylglucosaminidase and not a hyaluronidase. Biochem J (2006) 0.99
Structural and mechanistic insight into N-glycan processing by endo-α-mannosidase. Proc Natl Acad Sci U S A (2012) 0.98
Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum. Biochem J (2006) 0.98
An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis. Nat Chem Biol (2012) 0.98
Electrochemical method for the detection of lipase activity. Anal Chem (2005) 0.97
dUTPase as a platform for antimalarial drug design: structural basis for the selectivity of a class of nucleoside inhibitors. Structure (2005) 0.97
Understanding the plasticity of the alpha/beta hydrolase fold: lid swapping on the Candida antarctica lipase B results in chimeras with interesting biocatalytic properties. Chembiochem (2009) 0.97