Published in BMC Biotechnol on May 11, 2009
An automated system for high-throughput single cell-based breeding. Sci Rep (2013) 1.02
Efficient division and sampling of cell colonies using microcup arrays. Analyst (2012) 0.78
Powerful workhorses for antimicrobial peptide expression and characterization. Bioeng Bugs (2010) 0.78
Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci U S A (2000) 5.99
Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol (2004) 5.67
Rate of somatic mutation in immunoglobulin production by mouse myeloma cells. Proc Natl Acad Sci U S A (1971) 3.83
Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals. Appl Microbiol Biotechnol (2005) 2.04
A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity. J Immunol Methods (2003) 1.87
Selection methods for high-producing mammalian cell lines. Trends Biotechnol (2007) 1.35
The cumate gene-switch: a system for regulated expression in mammalian cells. BMC Biotechnol (2006) 1.26
Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnol Bioeng (2003) 1.26
Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells. Biotechnol Bioeng (1999) 1.26
Inducible overexpression of a toxic protein by an adenovirus vector with a tetracycline-regulatable expression cassette. J Virol (1998) 1.25
Multiplex detection and quantitation of proteins on western blots using fluorescent probes. Biotechniques (2000) 1.19
A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies. J Immunol Methods (1982) 1.18
Optimization of protein production in mammalian cells with a coexpressed fluorescent marker. Structure (2004) 1.16
Accelerated cell line development using two-color fluorescence activated cell sorting to select highly expressing antibody-producing clones. Biotechnol Bioeng (2008) 1.12
Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products. Biotechniques (1997) 1.11
Green fluorescent protein as a second selectable marker for selection of high producing clones from transfected CHO cells. Gene (2000) 1.09
Real-time monitoring of the interactions of two-stranded de novo designed coiled-coils: effect of chain length on the kinetic and thermodynamic constants of binding. Biochemistry (2003) 1.07
Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol (2004) 1.03
Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells. Biotechnol Bioeng (1998) 1.02
Recombinant therapeutic proteins: production platforms and challenges. Biotechnol J (2008) 1.02
Accelerated clone selection for recombinant CHO CELLS using a FACS-based high-throughput screen. Biotechnol Prog (2007) 0.98
Engineering mammalian cell factories for improved recombinant monoclonal antibody production: lessons from nature? Biotechnol Bioeng (2005) 0.96
Gel microdrop technology for rapid isolation of rare and high producer cells. Nat Med (1997) 0.94
Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting. Biotechnol Bioeng (2001) 0.93
Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors. J Immunol Methods (1999) 0.88
High-level recombinant protein production in CHO cells using an adenoviral vector and the cumate gene-switch. Biotechnol Prog (2007) 0.85
A ligand-pseudoreceptor system based on de novo designed peptides for the generation of adenoviral vectors with altered tropism. J Gene Med (2008) 0.83
Use of a micromanipulator for high-efficiency cloning of cells co-expressing fluorescent proteins. Methods Cell Sci (2000) 0.83
Effect of shear stress on intrinsic CHO culture state and glycosylation of recombinant tissue-type plasminogen activator protein. Biotechnol Prog (2003) 0.81
Automated in situ measurement of cell-specific antibody secretion and laser-mediated purification for rapid cloning of highly-secreting producers. Biotechnol Bioeng (2005) 0.80
Increased t-PA yields using ultrafiltration of an inhibitory product from CHO fed-batch culture. Biotechnol Prog (2000) 0.79
MUC16 (CA125) regulates epithelial ovarian cancer cell growth, tumorigenesis and metastasis. Gynecol Oncol (2011) 2.05
Polyploid megakaryocytes can complete cytokinesis. Cell Cycle (2010) 1.57
A role for the malonyl-CoA/long-chain acyl-CoA pathway of lipid signaling in the regulation of insulin secretion in response to both fuel and nonfuel stimuli. Diabetes (2004) 1.43
Nuclear retention of MBP mRNAs in the quaking viable mice. Neuron (2002) 1.41
Layer-by-layer films as a biomimetic reservoir for rhBMP-2 delivery: controlled differentiation of myoblasts to osteoblasts. Small (2009) 1.29
The cumate gene-switch: a system for regulated expression in mammalian cells. BMC Biotechnol (2006) 1.26
Distinct roles for IFN regulatory factor (IRF)-3 and IRF-7 in the activation of antitumor properties of human macrophages. Cancer Res (2006) 1.25
Inducible packaging cells for large-scale production of lentiviral vectors in serum-free suspension culture. Mol Ther (2008) 1.17
Translating the genomics revolution: the need for an international gene therapy consortium for monogenic diseases. Mol Ther (2013) 1.16
Dysregulated inflammatory response to Candida albicans in a C5-deficient mouse strain. Infect Immun (2004) 1.15
Adenoviral-mediated modulation of Sim1 expression in the paraventricular nucleus affects food intake. J Neurosci (2006) 1.14
The R1 subunit of herpes simplex virus ribonucleotide reductase protects cells against apoptosis at, or upstream of, caspase-8 activation. J Gen Virol (2002) 1.09
Asthma and the elite athlete: summary of the International Olympic Committee's consensus conference, Lausanne, Switzerland, January 22-24, 2008. J Allergy Clin Immunol (2008) 1.08
Factors influencing the efficacy, longevity, and safety of electroporation-assisted plasmid-based gene transfer into mouse muscles. Mol Ther (2004) 1.05
Production of lentiviral vectors by large-scale transient transfection of suspension cultures and affinity chromatography purification. Biotechnol Bioeng (2007) 1.02
Identification of host proteins associated with retroviral vector particles by proteomic analysis of highly purified vector preparations. J Virol (2007) 1.01
Variation of polyelectrolyte film stiffness by photo-cross-linking: a new way to control cell adhesion. Langmuir (2009) 1.00
Sustained improvement of muscle function one year after full-length dystrophin gene transfer into mdx mice by a gutted helper-dependent adenoviral vector. Hum Gene Ther (2004) 0.98
High-titer adenovirus vector production in 293S cell perfusion culture. Biotechnol Prog (2004) 0.97
The zinc-finger protein ZFR is critical for Staufen 2 isoform specific nucleocytoplasmic shuttling in neurons. J Neurochem (2005) 0.96
The ribonucleotide reductase R1 subunits of herpes simplex virus types 1 and 2 protect cells against TNFα- and FasL-induced apoptosis by interacting with caspase-8. Apoptosis (2011) 0.93
PABPN1 overexpression leads to upregulation of genes encoding nuclear proteins that are sequestered in oculopharyngeal muscular dystrophy nuclear inclusions. Neurobiol Dis (2005) 0.93
Oligodendrocyte injury in multiple sclerosis: a role for p53. J Neurochem (2003) 0.92
Novel, versatile, and tightly regulated expression system for Escherichia coli strains. Appl Environ Microbiol (2010) 0.92
Downstream processing of oncoretroviral and lentiviral gene therapy vectors. Biotechnol Adv (2006) 0.91
Efficient in vitro megakaryocyte maturation using cytokine cocktails optimized by statistical experimental design. Exp Hematol (2005) 0.91
Selection and tuning of a fast and simple phase-contrast microscopy image segmentation algorithm for measuring myoblast growth kinetics in an automated manner. Microsc Microanal (2013) 0.91
Bestowing inducibility on the cloned methanol dehydrogenase promoter (PmxaF) of Methylobacterium extorquens by applying regulatory elements of Pseudomonas putida F1. Appl Environ Microbiol (2006) 0.90
The ribonucleotide reductase domain of the R1 subunit of herpes simplex virus type 2 ribonucleotide reductase is essential for R1 antiapoptotic function. J Gen Virol (2007) 0.90
Peptidoglycan lytic activity of the Pseudomonas aeruginosa phage phiKZ gp144 lytic transglycosylase. FEMS Microbiol Lett (2007) 0.89
Megakaryocyte and platelet production from human cord blood stem cells. Methods Mol Biol (2012) 0.89
Factors associated with induced chronic inflammation in mdx skeletal muscle cause posttranslational stabilization and augmentation of extrasynaptic sarcolemmal utrophin. Hum Gene Ther (2005) 0.89
Overview of current scalable methods for purification of viral vectors. Methods Mol Biol (2011) 0.88
A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography. Biotechnol Bioeng (2005) 0.88
High-level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene-switch. Biotechnol Bioeng (2010) 0.87
MxA, a member of the dynamin superfamily, interacts with the ankyrin-like repeat domain of TRPC. J Biol Chem (2005) 0.87
Successful compensation for dystrophin deficiency by a helper-dependent adenovirus expressing full-length utrophin. Mol Ther (2007) 0.87
Single-cell level analysis of megakaryocyte growth and development. Differentiation (2012) 0.86
New developments in lentiviral vector design, production and purification. Expert Opin Biol Ther (2013) 0.86
Individual and synergistic cytokine effects controlling the expansion of cord blood CD34(+) cells and megakaryocyte progenitors in culture. Cytotherapy (2010) 0.85
Gene expression in HL60 granulocytoids and human polymorphonuclear leukocytes exposed to Candida albicans. Infect Immun (2004) 0.85
The R1 subunit of herpes simplex virus ribonucleotide reductase has chaperone-like activity similar to Hsp27. FEBS Lett (2003) 0.85
High-level recombinant protein production in CHO cells using an adenoviral vector and the cumate gene-switch. Biotechnol Prog (2007) 0.85
New protocol for lentiviral vector mass production. Methods Mol Biol (2010) 0.84
Cellular origins of adult human islet in vitro dedifferentiation. Lab Invest (2008) 0.83
Phosphatidylinositol 4,5-bisphosphate-induced conformational change of ezrin and formation of ezrin oligomers. Biochemistry (2010) 0.83
Alternative codon usage of PRRS virus ORF5 gene increases eucaryotic expression of GP(5) glycoprotein and improves immune response in challenged pigs. Vaccine (2005) 0.83
Targeting artificial transcription factors to the utrophin A promoter: effects on dystrophic pathology and muscle function. J Biol Chem (2008) 0.83
Purification and characterization of retrovirus vector particles by rate zonal ultracentrifugation. J Virol Methods (2005) 0.83
A ligand-pseudoreceptor system based on de novo designed peptides for the generation of adenoviral vectors with altered tropism. J Gene Med (2008) 0.83
Suicide gene therapy with an adenovirus expressing the fusion gene CD::UPRT in human glioblastomas: different sensitivities correlate with p53 status. J Gene Med (2004) 0.82
Ex vivo megakaryocyte expansion and platelet production from human cord blood stem cells. Methods Mol Biol (2009) 0.82
Retroviral vector production using suspension-adapted 293GPG cells in a 3L acoustic filter-based perfusion bioreactor. Biotechnol Bioeng (2006) 0.81
Polyelectrolyte multilayer nanofilms used as thin materials for cell mechano-sensitivity studies. Macromol Biosci (2011) 0.81
The dynamism of PABPN1 nuclear inclusions during the cell cycle. Neurobiol Dis (2006) 0.80
DNA delivery to cells in culture: generation of adenoviral libraries for high-throughput functional screening. Methods Mol Biol (2004) 0.80
Exploiting heparin-binding properties of MoMLV-based retroviral vectors for affinity chromatography. J Chromatogr B Analyt Technol Biomed Life Sci (2006) 0.80
Nano-scale control of cellular environment to drive embryonic stem cells selfrenewal and fate. Biomaterials (2009) 0.80
An improved mouse model for endometriosis allows noninvasive assessment of lesion implantation and development. Fertil Steril (2003) 0.78
Cell culture tracking by multivariate analysis of raw LCMS data. Appl Biochem Biotechnol (2012) 0.78
A noninvasive cell-based assay for monitoring proteolytic activity within a specific subcellular compartment. Anal Biochem (2002) 0.78
Multilayer assembly of hyaluronic acid/poly(allylamine): control of the buildup for the production of hollow capsules. Langmuir (2008) 0.78
Development of BMP7-producing human cells, using a third generation lentiviral gene delivery system. J Neurosci Methods (2011) 0.78
HLA-DO transduced in human monocyte-derived dendritic cells modulates MHC class II antigen processing. J Leukoc Biol (2005) 0.78
Flagellin in fusion with human rotavirus structural proteins exerts an adjuvant effect when delivered with replicating but non-disseminating adenovectors through the intrarectal route. Mol Biotechnol (2014) 0.77
The bovine viral diarrhea virus (BVDV) NS3 protein, when expressed alone in mammalian cells, induces apoptosis which correlates with caspase-8 and caspase-9 activation. Vet Res (2005) 0.77
Strong muscle-specific regulatory cassettes based on multiple copies of the human slow troponin I gene upstream enhancer. Hum Gene Ther (2010) 0.77
Multivariate analysis of single quadrupole LC-MS spectra for routine characterization and quantification of intact proteins. Proteomics (2009) 0.77
Differentiation of murine embryonic stem cells in skeletal muscles of mice. Cell Transplant (2008) 0.77
Modulation of coxsackie and adenovirus receptor expression for gene transfer to normal and dystrophic skeletal muscle. J Gene Med (2010) 0.77
Efficacy and site-specificity of adenoviral vector integration mediated by the phage φC31 integrase. Hum Gene Ther Methods (2012) 0.76
Production and characterization of the recombinant Islet Neogenesis Associated Protein (rINGAP). Protein Expr Purif (2009) 0.76
Enzymatic assays for probing mitochondrial apoptosis. Methods Mol Biol (2015) 0.76
Escherichia coli expression and refolding of E/K-coil-tagged EGF generates fully bioactive EGF for diverse applications. Protein Expr Purif (2008) 0.76
Adenovirus-delivered antisense RNA and shRNA exhibit different silencing efficiencies for the endogenous transforming growth factor-beta (TGF-beta) type II receptor. Oligonucleotides (2006) 0.76
Improvement of antitumor activity by gene amplification with a replicating but nondisseminating adenovirus. Cancer Res (2007) 0.76
Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions. J Virol Methods (2008) 0.76
Different sites of xenoantigen delivery lead to a virally induced late-onset hepatitis in mice through molecular mimicry. Liver Int (2011) 0.76
Alkylamino hydrazide derivatives of hyaluronic acid: synthesis, characterization in semidilute aqueous solutions, and assembly into thin multilayer films. Biomacromolecules (2009) 0.76
Strength and muscle specificity of a compact promoter derived from the slow troponin I gene in the context of episomal (gutless adenovirus) and integrating (lentiviral) vectors. J Gene Med (2012) 0.76
Purification of retrovirus particles using heparin affinity chromatography. Methods Mol Biol (2008) 0.76
PH, pCO2, and temperature effect on R-adenovirus production. Biotechnol Prog (2003) 0.76
A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces. Biomaterials (2013) 0.76
On the proper use of statistical design of experiments. Exp Hematol (2005) 0.75
Expression of human rotavirus chimeric fusion proteins from replicating but non disseminating adenovectors and elicitation of rotavirus-specific immune responses in mice. Mol Biotechnol (2013) 0.75
[The care of the burn patient in the rehabilitation center]. Soins (2012) 0.75
A simple macroscopic model for the diffusion and adsorption kinetics of r-adenovirus. Biotechnol Bioeng (2007) 0.75