Quantitative evaluation of DNA methylation by optimization of a differential-high resolution melt analysis protocol.

PubWeight™: 0.95‹?› | Rank: Top 15%

🔗 View Article (PMC 2709587)

Published in Nucleic Acids Res on May 19, 2009

Authors

Francesca Malentacchi1, Giulia Forni, Serena Vinci, Claudio Orlando

Author Affiliations

1: Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy.

Articles cited by this

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods (2001) 414.27

DNA methylation patterns and epigenetic memory. Genes Dev (2002) 33.83

The fundamental role of epigenetic events in cancer. Nat Rev Genet (2002) 30.05

Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A (1996) 29.54

Gene silencing in cancer in association with promoter hypermethylation. N Engl J Med (2003) 18.04

The history of cancer epigenetics. Nat Rev Cancer (2004) 14.38

MethyLight: a high-throughput assay to measure DNA methylation. Nucleic Acids Res (2000) 9.30

High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem (2003) 7.77

DNA methylation and gene silencing in cancer. Nat Clin Pract Oncol (2005) 5.02

Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin Chem (2004) 3.99

Gene-promoter hypermethylation as a biomarker in lung cancer. Nat Rev Cancer (2004) 3.10

Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res (2007) 2.69

Methylation-sensitive high-resolution melting-curve analysis of the SNRPN gene as a diagnostic screen for Prader-Willi and Angelman syndromes. Clin Chem (2007) 2.14

High resolution melting applications for clinical laboratory medicine. Exp Mol Pathol (2008) 1.92

CpG island methylator phenotypes in aging and cancer. Semin Cancer Biol (1999) 1.79

Detection of methylated apoptosis-associated genes in urine sediments of bladder cancer patients. Clin Cancer Res (2004) 1.69

Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection. Nucleic Acids Res (2008) 1.57

In-tube DNA methylation profiling by fluorescence melting curve analysis. Clin Chem (2001) 1.51

BRCA1 promoter methylation in peripheral blood DNA of mutation negative familial breast cancer patients with a BRCA1 tumour phenotype. Breast Cancer Res (2008) 1.30

Aberrant DNA methylation in cancer: potential clinical interventions. Expert Rev Mol Med (2002) 1.23

High quality assessment of DNA methylation in archival tissues from colorectal cancer patients using quantitative high-resolution melting analysis. J Mol Diagn (2009) 1.21

Quantitative assessment of DNA methylation: Potential applications for disease diagnosis, classification, and prognosis in clinical settings. J Mol Med (Berl) (2006) 1.16

Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR. BMC Cancer (2008) 1.12

Profiling DNA methylation by melting analysis. Methods (2002) 1.12

Rapid detection of methylation change at H19 in human imprinting disorders using methylation-sensitive high-resolution melting. Hum Mutat (2008) 1.06

Methods for detecting DNA methylation in tumors: from bench to bedside. Cancer Lett (2006) 0.99

Articles by these authors

Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem (2002) 4.84

Application of a filtration- and isolation-by-size technique for the detection of circulating tumor cells in cutaneous melanoma. J Invest Dermatol (2010) 3.26

An alternatively spliced variant of CXCR3 mediates the inhibition of endothelial cell growth induced by IP-10, Mig, and I-TAC, and acts as functional receptor for platelet factor 4. J Exp Med (2003) 2.91

High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer. Am J Clin Pathol (2008) 2.46

Metalloproteinases-2, -9 and TIMP-1 expression in stable and unstable coronary plaques undergoing PCI. Int J Cardiol (2007) 2.05

Circulating benign nevus cells detected by ISET technique: warning for melanoma molecular diagnosis. Arch Dermatol (2010) 1.46

Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase-polymerase chain reaction results and feasibility of molecular analysis by laser microdissection. Hum Pathol (2006) 1.28

The use of COLD-PCR and high-resolution melting analysis improves the limit of detection of KRAS and BRAF mutations in colorectal cancer. J Mol Diagn (2010) 1.26

Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa. Endocrinology (2004) 1.25

Circulating nucleic acids in cancer and pregnancy. Methods (2010) 1.22

EQUAL-quant: an international external quality assessment scheme for real-time PCR. Clin Chem (2006) 1.20

Genetic variants in miR-146a, miR-149, miR-196a2, miR-499 and their influence on relative expression in lung cancers. Clin Chem Lab Med (2011) 1.09

Genetic and epigenetic factors in regulation of microRNA in colorectal cancers. Methods (2012) 1.07

Association between single nucleotide polymorphisms in the XRCC1 and RAD51 genes and clinical radiosensitivity in head and neck cancer. Radiother Oncol (2011) 1.04

Tankyrase, a positive regulator of telomere elongation, is over expressed in human breast cancer. Cancer Lett (2004) 0.98

BRAFV600E detection in melanoma is highly improved by COLD-PCR. Clin Chim Acta (2011) 0.98

The CXCR4/CXCL12 axis in endometrial cancer. Clin Exp Metastasis (2009) 0.97

Multiparametric analysis of cell-free DNA in melanoma patients. PLoS One (2012) 0.97

Circulating cell-free DNA in plasma of melanoma patients: qualitative and quantitative considerations. Clin Chim Acta (2011) 0.96

Analysis of gene expression profiles reveals novel correlations with the clinical course of colorectal cancer. Oncol Res (2007) 0.94

The Integrated Oncology Program of the Italian Ministry of Health. Analytical and clinical validation of new biomarkers for early diagnosis: network, resources, methodology, quality control, and data analysis. Int J Biol Markers (2009) 0.93

VEGF expression in the placenta from pregnancies complicated by hypertensive disorders. BJOG (2004) 0.92

Up-regulated expression of low molecular weight protein tyrosine phosphatases in different human cancers. Biochem Biophys Res Commun (2005) 0.90

Quantitative methylation analysis of BCL2, hTERT, and DAPK promoters in urine sediment for the detection of non-muscle-invasive urothelial carcinoma of the bladder: a prospective, two-center validation study. Urol Oncol (2009) 0.90

Quantitative evaluation of somatostatin receptor subtype 2 expression in sporadic colorectal tumor and in the corresponding normal mucosa. Clin Cancer Res (2002) 0.89

Alternative splicing variants of carbonic anhydrase IX in human non-small cell lung cancer. Lung Cancer (2008) 0.89

Simultaneous measurement of MMP9 and TIMP1 mRNA in human non small cell lung cancers by multiplex real time RT-PCR. Lung Cancer (2004) 0.88

Atypical Spitzoid melanocytic tumors: a morphological, mutational, and FISH analysis. J Am Acad Dermatol (2011) 0.88

Rosiglitazone impairs proliferation of human adrenocortical cancer: preclinical study in a xenograft mouse model. Endocr Relat Cancer (2010) 0.87

Tankyrase-1 mRNA expression in bladder cancer and paired urine sediment: preliminary experience. Clin Chem Lab Med (2007) 0.87

Distribution of Tankyrase-1 mRNA expression in colon cancer and its prospective correlation with progression stage. Oncol Rep (2006) 0.87

High resolution melting analysis for a rapid identification of heterozygous and homozygous sequence changes in the MUTYH gene. BMC Cancer (2011) 0.86

Quantitative analysis of carbonic anhydrase IX mRNA in human non-small cell lung cancer. Lung Cancer (2006) 0.86

Tyrosinase mRNA levels in the blood of uveal melanoma patients: correlation with the number of circulating tumor cells and tumor progression. Melanoma Res (2010) 0.85

NF2 mutation screening by denaturing high-performance liquid chromatography and high-resolution melting analysis. Genet Test (2008) 0.85

Evaluation of cell-free DNA in urine as a marker for bladder cancer diagnosis. Int J Biol Markers (2009) 0.84

CXCL9 and CXCL11 chemokines modulation by peroxisome proliferator-activated receptor-alpha agonists secretion in Graves' and normal thyrocytes. J Clin Endocrinol Metab (2010) 0.84

External quality assurance program for PCR amplification of genomic DNA: an Italian experience. Clin Chem (2003) 0.83

BRAF and KIT somatic mutations are present in amelanotic melanoma. Melanoma Res (2013) 0.83

Allele specific Taqman-based real-time PCR assay to quantify circulating BRAFV600E mutated DNA in plasma of melanoma patients. Clin Chim Acta (2010) 0.83

Oxytocin receptor is expressed in the penis and mediates an estrogen-dependent smooth muscle contractility. Endocrinology (2003) 0.82

Splicing variants of carbonic anhydrase IX in bladder cancer and urine sediments. Urol Oncol (2010) 0.81

Monitoring the susceptibility to oseltamivir of Influenza A(H1N1) 2009 virus by nested-PCR and pyrosequencing during the pandemic and in the season 2010-2011. J Virol Methods (2012) 0.81

Influence of storage conditions and extraction methods on the quantity and quality of circulating cell-free DNA (ccfDNA): the SPIDIA-DNAplas External Quality Assessment experience. Clin Chem Lab Med (2015) 0.80

GM1 gangliosidosis: molecular analysis of nine patients and development of an RT-PCR assay for GLB1 gene expression profiling. Hum Mutat (2007) 0.80

Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reaction. Hum Pathol (2008) 0.80

Stem cell factor affects tumour progression markers in metastatic melanoma cells. Clin Exp Metastasis (2006) 0.79

From clinical trials to clinical practice: predictors of response to erlotinib in advanced non-small cell lung cancer patients pretreated with chemotherapy. Tumori (2011) 0.79

Circulating cell-free DNA in cancer. Methods Mol Biol (2014) 0.79

Real-time RT-PCR for the measurement of prostate-specific antigen mRNA expression in benign hyperplasia and adenocarcinoma of prostate. Clin Chem Lab Med (2003) 0.79

An Italian program of external quality control for quantitative assays based on real-time PCR with Taq-Man probes. Clin Chem Lab Med (2005) 0.79

Evaluation of a panel of circulating DNA, RNA and protein potential markers for pathologies of pregnancy. Clin Chem Lab Med (2010) 0.78

A high-resolution melting protocol for rapid and accurate differential diagnosis of thyroid nodules. J Mol Diagn (2012) 0.78

Seladin-1 expression is regulated by promoter methylation in adrenal cancer. BMC Cancer (2010) 0.78

Dysregulation of secretion of CXC alpha-chemokine CXCL10 in papillary thyroid cancer: modulation by peroxisome proliferator-activated receptor-gamma agonists. Endocr Relat Cancer (2009) 0.78

A new microarray substrate for ultra-sensitive genotyping of KRAS and BRAF gene variants in colorectal cancer. PLoS One (2013) 0.75

Genomic rearrangements of the CDKN2A locus are infrequent in Italian malignant melanoma families without evidence of CDKN2A/CDK4 point mutations. Melanoma Res (2008) 0.75

Research trends for early cancer biomarker detection in Italy: an integrated program in oncology (PIO) survey. Tumori (2011) 0.75

Development and validation of a 2nd tier test for identification of purine nucleoside phosphorylase deficiency patients during expanded newborn screening by liquid chromatography-tandem mass spectrometry. Clin Chem Lab Med (2016) 0.75

Bivariate statistical approach to evaluate laboratory performance by analysis of standard curves in an External Quality Assurance program for quantitative assays based on real-time PCR with Taq-Man probes. Clin Chem Lab Med (2006) 0.75

Influence of 17q gain and promoter polymorphisms on mRNA expression of somatostatin receptor type 2 in neuroblastoma. Clin Chim Acta (2007) 0.75

Prognostic value of somatostatin receptor subtype 2 expression in colorectal cancer. Regul Pept (2005) 0.75

External quality assessment schemes for real-time PCR: a statistical procedure to corrective actions. Clin Chem Lab Med (2008) 0.75

Decision criteria for rational selection of homogeneous genotyping platforms for pharmacogenomics testing in clinical diagnostics. Clin Chem Lab Med (2010) 0.75

Macrobenthos community structural changes off Cesenatico coast (Emilia Romagna, Northern Adriatic), a six-year monitoring programme. Sci Total Environ (2005) 0.75

Critical appraisal on the identification of Reference Conditions for the evaluation of ecological quality status along the Emilia-Romagna coast (Italy) using M-AMBI. Mar Pollut Bull (2011) 0.75

Two novel H-RAS mutations identified in a child with an atypical spitzoid tumor. Arch Dermatol (2011) 0.75

Comparison of pre- and postsurgical concentrations of blood HER-2 mRNA and HER-2 extracellular domain reflects HER-2 status in early breast cancer. Clin Chem (2005) 0.75