Published in Nucleic Acids Res on June 15, 2009
Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling. Mol Brain (2011) 0.88
In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display. Proc Natl Acad Sci U S A (2012) 0.84
Peptide aptamers: development and applications. Curr Top Med Chem (2015) 0.80
In vitro selection of proteins with desired characteristics using mRNA-display. Methods (2012) 0.78
Enhanced mRNA-protein fusion efficiency of a single-domain antibody by selection of mRNA display with additional random sequences in the terminal translated regions. Biophys Physicobiol (2017) 0.75
Easy and rapid binding assay for functional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system. Biology (Basel) (2015) 0.75
Increasing the library size in cDNA display by optimizing purification procedures. Biol Proced Online (2013) 0.75
Establishment of a novel cell-based assay for screening small molecule antagonists of human interleukin-6 receptor. Acta Pharmacol Sin (2014) 0.75
Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J Immunol Methods (1985) 6.11
RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc Natl Acad Sci U S A (1997) 5.06
In vitro selection and evolution of functional proteins by using ribosome display. Proc Natl Acad Sci U S A (1997) 4.71
Phage Display. Chem Rev (1997) 4.57
Engineering novel binding proteins from nonimmunoglobulin domains. Nat Biotechnol (2005) 3.67
Three-dimensional solution structure of the B domain of staphylococcal protein A: comparisons of the solution and crystal structures. Biochemistry (1992) 3.20
An in vitro polysome display system for identifying ligands from very large peptide libraries. Proc Natl Acad Sci U S A (1994) 2.52
In vitro virus: bonding of mRNA bearing puromycin at the 3'-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro. FEBS Lett (1997) 2.40
The DNA binding specificity of the bipartite POU domain and its subdomains. EMBO J (1992) 2.36
Solution structure of the POU-specific DNA-binding domain of Oct-1. Nature (1993) 1.79
In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods (2004) 1.69
Scanning the human proteome for calmodulin-binding proteins. Proc Natl Acad Sci U S A (2005) 1.49
Psoralen photo-crosslinked mRNA-puromycin conjugates: a novel template for the rapid and facile preparation of mRNA-protein fusions. Nucleic Acids Res (2000) 1.32
Highly stable and efficient mRNA templates for mRNA-protein fusions and C-terminally labeled proteins. Nucleic Acids Res (2003) 1.31
A new generation of protein display scaffolds for molecular recognition. Protein Sci (2006) 1.29
Antibodies selected from combinatorial libraries block a tumor antigen that plays a key role in immunomodulation. Proc Natl Acad Sci U S A (2006) 1.28
Fluorescence labeling of the C-terminus of proteins with a puromycin analogue in cell-free translation systems. FEBS Lett (1999) 1.27
In vitro selection of state-specific peptide modulators of G protein signaling using mRNA display. Biochemistry (2004) 1.21
Design of synthetic gene libraries encoding random sequence proteins with desired ensemble characteristics. Protein Sci (1993) 1.16
Primary and secondary structure of conotoxin GI, a neurotoxic tridecapeptide from a marine snail. FEBS Lett (1982) 1.12
An in vitro DNA virus for in vitro protein evolution. FEBS Lett (2001) 1.10
cDNA - protein fusions: covalent protein - gene conjugates for the in vitro selection of peptides and proteins. Chembiochem (2001) 1.07
Rapid preparation of single stranded DNA from PCR products by streptavidin induced electrophoretic mobility shift. Nucleic Acids Res (1996) 1.01
A novel class of small functional peptides that bind and inhibit human alpha-thrombin isolated by mRNA display. Chem Biol (2003) 1.00
Y-ligation: an efficient method for ligating single-stranded DNAs and RNAs with T4 RNA ligase. Mol Divers (1998) 0.98
Isolation of cell surface-specific human monoclonal antibodies using phage display and magnetically-activated cell sorting: applications in immunohematology. J Immunol Methods (1997) 0.96
mRNA display: diversity matters during in vitro selection. Proc Natl Acad Sci U S A (2001) 0.92
Solid-phase translation and RNA-protein fusion: a novel approach for folding quality control and direct immobilization of proteins using anchored mRNA. Nucleic Acids Res (2006) 0.85
Generating addressable protein microarrays with PROfusion covalent mRNA-protein fusion technology. Proteomics (2002) 0.84
Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature (2005) 8.55
Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature (2005) 7.74
System-level identification of transcriptional circuits underlying mammalian circadian clocks. Nat Genet (2005) 5.10
Microscopic analysis of polymerization dynamics with individual actin filaments. Nat Cell Biol (2002) 3.41
Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome. Proc Natl Acad Sci U S A (2002) 2.63
Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy. Nat Commun (2012) 2.47
Hydrophilic fluorescent nanogel thermometer for intracellular thermometry. J Am Chem Soc (2009) 1.90
Infrared laser-mediated gene induction in targeted single cells in vivo. Nat Methods (2008) 1.78
Efficacy and safety of palliative sedation therapy: a multicenter, prospective, observational study conducted on specialized palliative care units in Japan. J Pain Symptom Manage (2005) 1.57
Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae. Appl Microbiol Biotechnol (2005) 1.45
Kinetic study of de novo chromophore maturation of fluorescent proteins. Anal Biochem (2011) 1.37
GroEL mediates protein folding with a two successive timer mechanism. Mol Cell (2004) 1.36
Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice. Biochem Biophys Res Commun (2008) 1.29
Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tags. Appl Microbiol Biotechnol (2004) 1.24
Genome Sequence of the Basidiomycetous Yeast Pseudozyma antarctica T-34, a Producer of the Glycolipid Biosurfactants Mannosylerythritol Lipids. Genome Announc (2013) 1.20
Genomics of Aspergillus oryzae. Biosci Biotechnol Biochem (2007) 1.20
kappa-Hefutoxin1, a novel toxin from the scorpion Heterometrus fulvipes with unique structure and function. Importance of the functional diad in potassium channel selectivity. J Biol Chem (2002) 1.17
Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions. DNA Res (2007) 1.14
Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells: nuclear mRNA export is coupled to ongoing gene transcription. Genes Cells (2006) 1.10
Low density of sigma1 receptors in early Alzheimer's disease. Ann Nucl Med (2008) 1.07
Spontaneous muscle action potentials fail to develop without fetal-type acetylcholine receptors. EMBO Rep (2002) 1.06
Plasmid-encoded asp operon confers a proton motive metabolic cycle catalyzed by an aspartate-alanine exchange reaction. J Bacteriol (2002) 1.06
Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivation. Fungal Genet Biol (2007) 1.03
Quantum dot FRET biosensors that respond to pH, to proteolytic or nucleolytic cleavage, to DNA synthesis, or to a multiplexing combination. J Am Chem Soc (2008) 1.03
Interleukin-18 promoter polymorphisms and the disease progression of Hepatitis B virus-related liver disease. Transl Res (2008) 1.02
Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae. Fungal Genet Biol (2010) 1.01
Football- and bullet-shaped GroEL-GroES complexes coexist during the reaction cycle. J Biol Chem (2008) 1.01
Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai. Proc Natl Acad Sci U S A (2008) 1.01
Effect of the C-terminal truncation on the functional cycle of chaperonin GroEL: implication that the C-terminal region facilitates the transition from the folding-arrested to the folding-competent state. J Biol Chem (2008) 1.01
Aspergillus luchuensis, an industrially important black Aspergillus in East Asia. PLoS One (2013) 1.00
Denatured proteins facilitate the formation of the football-shaped GroEL-(GroES)2 complex. Biochem J (2010) 0.98
Nanoscale patterning of protein using electron beam lithography of organosilane self-assembled monolayers. Small (2005) 0.97
Single-molecule study on the decay process of the football-shaped GroEL-GroES complex using zero-mode waveguides. J Biol Chem (2010) 0.96
On-chip cell sorting system using laser-induced heating of a thermoreversible gelation polymer to control flow. Anal Chem (2006) 0.96
Single-molecule imaging of beta-actin mRNAs in the cytoplasm of a living cell. Exp Cell Res (2009) 0.96
Impact of Aspergillus oryzae genomics on industrial production of metabolites. Mycopathologia (2006) 0.96
Experimental rugged fitness landscape in protein sequence space. PLoS One (2006) 0.96
Single-molecule imaging of full protein synthesis by immobilized ribosomes. Nucleic Acids Res (2008) 0.95
Fine de novo sequencing of a fungal genome using only SOLiD short read data: verification on Aspergillus oryzae RIB40. PLoS One (2013) 0.93
An Efficient Ligation Method in the Making of an in vitro Virus for in vitro Protein Evolution. Biol Proced Online (2002) 0.93
Single molecule tracking of quantum dot-labeled mRNAs in a cell nucleus. Biochem Biophys Res Commun (2009) 0.93
Kojic acid biosynthesis in Aspergillus oryzae is regulated by a Zn(II)(2)Cys(6) transcriptional activator and induced by kojic acid at the transcriptional level. J Biosci Bioeng (2011) 0.93
Greater adenosine A(2A) receptor densities in cardiac and skeletal muscle in endurance-trained men: a [11C]TMSX PET study. Nucl Med Biol (2005) 0.92
A polymerase chain reaction-based method for cloning novel members of a gene family using a combination of degenerate and inhibitory primers. Gene (2002) 0.91
Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice. Biochem Biophys Res Commun (2008) 0.91
Real-time imaging of single-molecule fluorescence with a zero-mode waveguide for the analysis of protein-protein interaction. Anal Chem (2008) 0.91
Development of systemic in vitro evolution and its application to generation of peptide-aptamer-based inhibitors of cathepsin E. J Mol Biol (2008) 0.91
Construction of block-shuffled libraries of DNA for evolutionary protein engineering: Y-ligation-based block shuffling. Protein Eng (2002) 0.90
Surveying a local fitness landscape of a protein with epistatic sites for the study of directed evolution. Biopolymers (2002) 0.89
Generation of large chromosomal deletions in koji molds Aspergillus oryzae and Aspergillus sojae via a loop-out recombination. Appl Environ Microbiol (2008) 0.89
Increased production of fatty acids and triglycerides in Aspergillus oryzae by enhancing expressions of fatty acid synthesis-related genes. Appl Microbiol Biotechnol (2012) 0.89
A database for the provisional identification of species using only genotypes: web-based genome profiling. Genome Biol (2002) 0.89
On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation. Lab Chip (2009) 0.89
Dynamic association-dissociation and harboring of endogenous mRNAs in stress granules. J Cell Sci (2011) 0.88
Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling. Mol Brain (2011) 0.88
Selective detection and transport of fully matched DNA by DNA-loaded microtubule and kinesin motor protein. Biotechnol Bioeng (2006) 0.88
Single-molecule observation of protein folding in symmetric GroEL-(GroES)2 complexes. J Biol Chem (2012) 0.88
Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2'-O-methyl RNA probes in living cells. Nucleic Acids Res (2010) 0.88
Imaging of single mRNA molecules moving within a living cell nucleus. Biochem Biophys Res Commun (2006) 0.87
Cellular responses of the ciliate, Tetrahymena thermophila, to far infrared irradiation. Photochem Photobiol Sci (2006) 0.87
Brain histamine H receptor occupancy of orally administered antihistamines measured by positron emission tomography with (11)C-doxepin in a placebo-controlled crossover study design in healthy subjects: a comparison of olopatadine and ketotifen. Br J Clin Pharmacol (2006) 0.87
Dynamical properties of autoimmune disease models: tolerance, flare-up, dormancy. J Theor Biol (2007) 0.87
The dynamics of pre-mRNAs and poly(A)+ RNA at speckles in living cells revealed by iFRAP studies. Exp Cell Res (2007) 0.86
Size-dependent accumulation of mRNA at the leading edge of chicken embryo fibroblasts. Biochem Biophys Res Commun (2009) 0.86
One-pot preparation of mRNA/cDNA display by a novel and versatile puromycin-linker DNA. ACS Comb Sci (2011) 0.86
Size distribution of linear and helical polymers in actin solution analyzed by photon counting histogram. Biophys J (2006) 0.86
Structure and chromosomal localization of the gene for crotamine, a toxin from the South American rattlesnake, Crotalus durissus terrificus. Toxicon (2003) 0.85
Solid-phase translation and RNA-protein fusion: a novel approach for folding quality control and direct immobilization of proteins using anchored mRNA. Nucleic Acids Res (2006) 0.85
Aspergillus flavus genomics as a tool for studying the mechanism of aflatoxin formation. Food Addit Contam Part A Chem Anal Control Expo Risk Assess (2008) 0.85
Thermodynamical interpretation of an adaptive walk on a Mt. Fuji-type fitness landscape: Einstein relation-like formula holds in a stochastic evolution. J Theor Biol (2003) 0.85
Adenosine A1 receptor mapping of the human brain by PET with 8-dicyclopropylmethyl-1-11C-methyl-3-propylxanthine. J Nucl Med (2005) 0.85
Stability analysis of pathogen-immune interaction dynamics. J Math Biol (2005) 0.85
An mRNA-protein fusion at N-terminus for evolutionary protein engineering. Int J Biol Sci (2007) 0.84
In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display. Proc Natl Acad Sci U S A (2012) 0.84
Label-free DNA sensors using ultrasensitive diamond field-effect transistors in solution. Phys Rev E Stat Nonlin Soft Matter Phys (2006) 0.84
Substrate ectodomain is critical for substrate preference and inhibition of γ-secretase. Nat Commun (2013) 0.84
Modified substrate specificity of pyrroloquinoline quinone glucose dehydrogenase by biased mutation assembling with optimized amino acid substitution. Appl Microbiol Biotechnol (2006) 0.84
Biocompatible, surface functionalized mesoporous titania nanoparticles for intracellular imaging and anticancer drug delivery. Chem Commun (Camb) (2011) 0.83
Selection-by-function: efficient enrichment of cathepsin E inhibitors from a DNA library. J Mol Recognit (2007) 0.83
First visualization of adenosine A(2A) receptors in the human brain by positron emission tomography with [11C]TMSX. Synapse (2005) 0.83
Identification of a basic helix-loop-helix-type transcription regulator gene in Aspergillus oryzae by systematically deleting large chromosomal segments. Appl Environ Microbiol (2009) 0.83
Single-molecule fluorescence polarization study of conformational change in archaeal group II chaperonin. PLoS One (2011) 0.83
Successive positron emission tomography measurement of cerebral blood flow and neuroreceptors in the human brain: an 11C-SA4503 study. Ann Nucl Med (2008) 0.83
Synthesis of benzofurazan derivatization reagents for carboxylic acids and its application to analysis of fatty acids in rat plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry. Biomed Chromatogr (2005) 0.83
Malachite green-conjugated microtubules as mobile bioprobes selective for malachite green aptamers with capturing/releasing ability. Biotechnol Bioeng (2006) 0.83
p38 MAPKs regulate the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors. J Cell Sci (2011) 0.83
Determination of gene copy number and genotype of transgenic Arabidopsis thaliana by competitive PCR. J Exp Bot (2002) 0.82
Extracting characteristic properties of fitness landscape from in vitro molecular evolution: a case study on infectivity of fd phage to E.coli. J Theor Biol (2007) 0.82
cDNA display: rapid stabilization of mRNA display. Methods Mol Biol (2012) 0.82
Thermodynamical interpretation of evolutionary dynamics on a fitness landscape in a evolution reactor, I. Bull Math Biol (2004) 0.82