PubRank

J J Mekalanos

Author PubWeight™ 436.63‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 1988 31.85
2 DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 2000 19.93
3 Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci U S A 1987 15.50
4 Lysogenic conversion by a filamentous phage encoding cholera toxin. Science 1996 15.37
5 Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature 1984 12.10
6 Cholera toxin transcriptional activator toxR is a transmembrane DNA binding protein. Cell 1987 11.04
7 Coordinate regulation and sensory transduction in the control of bacterial virulence. Science 1989 10.59
8 A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence. Proc Natl Acad Sci U S A 1989 9.79
9 Toxin, toxin-coregulated pili, and the toxR regulon are essential for Vibrio cholerae pathogenesis in humans. J Exp Med 1988 9.19
10 Duplication and amplification of toxin genes in Vibrio cholerae. Cell 1983 8.53
11 Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR. Proc Natl Acad Sci U S A 1984 8.47
12 Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae. Microbiol Mol Biol Rev 1998 8.36
13 Selection of bacterial virulence genes that are specifically induced in host tissues. Science 1993 6.70
14 Regulatory cascade controls virulence in Vibrio cholerae. Proc Natl Acad Sci U S A 1991 6.29
15 Iron regulation of Shiga-like toxin expression in Escherichia coli is mediated by the fur locus. J Bacteriol 1987 6.13
16 Broad-host-range vectors for delivery of TnphoA: use in genetic analysis of secreted virulence determinants of Vibrio cholerae. J Bacteriol 1989 5.85
17 In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria. Proc Natl Acad Sci U S A 1999 4.97
18 Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages. J Bacteriol 1990 4.87
19 Characterization of the Vibrio cholerae ToxR regulon: identification of novel genes involved in intestinal colonization. Infect Immun 1988 4.83
20 Genetics of bacterial enterotoxins. Annu Rev Microbiol 1986 4.63
21 TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A 1998 4.35
22 Alterations in Vibrio cholerae motility phenotypes correlate with changes in virulence factor expression. Infect Immun 1996 4.14
23 Type VI secretion requires a dynamic contractile phage tail-like structure. Nature 2012 3.88
24 A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139. J Bacteriol 1996 3.88
25 Two trans-acting regulatory genes (vir and mod) control antigenic modulation in Bordetella pertussis. J Bacteriol 1988 3.88
26 Systematic identification of essential genes by in vitro mariner mutagenesis. Proc Natl Acad Sci U S A 1998 3.86
27 Identification of a vibrio cholerae RTX toxin gene cluster that is tightly linked to the cholera toxin prophage. Proc Natl Acad Sci U S A 1999 3.75
28 Use of recombinase gene fusions to identify Vibrio cholerae genes induced during infection. Mol Microbiol 1995 3.69
29 Molecular cloning of Vibrio cholerae enterotoxin genes in Escherichia coli K-12. Proc Natl Acad Sci U S A 1982 3.68
30 Alkaline phosphatase fusions: sensors of subcellular location. J Bacteriol 1990 3.59
31 Hyperactive transposase mutants of the Himar1 mariner transposon. Proc Natl Acad Sci U S A 1999 3.45
32 CTX genetic element encodes a site-specific recombination system and an intestinal colonization factor. Proc Natl Acad Sci U S A 1993 3.34
33 Nucleotide sequence of the type A staphylococcal enterotoxin gene. J Bacteriol 1988 3.26
34 Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli. Proc Natl Acad Sci U S A 1987 3.25
35 Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol Microbiol 1998 3.24
36 Regulation, replication, and integration functions of the Vibrio cholerae CTXphi are encoded by region RS2. Mol Microbiol 1997 3.22
37 Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. Infect Immun 1999 3.10
38 Periplasmic interaction between two membrane regulatory proteins, ToxR and ToxS, results in signal transduction and transcriptional activation. Cell 1991 3.09
39 ToxR regulates virulence gene expression in non-O1 strains of Vibrio cholerae that cause epidemic cholera. Infect Immun 1994 3.08
40 Genetic regulation of bacterial virulence. Annu Rev Genet 1989 2.98
41 Enzymic activity of cholera toxin. II. Relationships to proteolytic processing, disulfide bond reduction, and subunit composition. J Biol Chem 1979 2.93
42 Isolation of enterotoxin structural gene deletion mutations in Vibrio cholerae induced by two mutagenic vibriophages. Proc Natl Acad Sci U S A 1982 2.92
43 Affinity filters, a new approach to the isolation of tox mutants of Vibrio cholerae. Proc Natl Acad Sci U S A 1978 2.89
44 Identification of toxS, a regulatory gene whose product enhances toxR-mediated activation of the cholera toxin promoter. J Bacteriol 1989 2.87
45 Distinct roles of an alternative sigma factor during both free-swimming and colonizing phases of the Vibrio cholerae pathogenic cycle. Mol Microbiol 1998 2.74
46 The Vibrio cholerae O139 serogroup antigen includes an O-antigen capsule and lipopolysaccharide virulence determinants. Proc Natl Acad Sci U S A 1994 2.68
47 Emergence of a new cholera pandemic: molecular analysis of virulence determinants in Vibrio cholerae O139 and development of a live vaccine prototype. J Infect Dis 1994 2.67
48 Monoclonal secretory immunoglobulin A protects mice against oral challenge with the invasive pathogen Salmonella typhimurium. Infect Immun 1992 2.66
49 Genetic analysis of the cholera toxin-positive regulatory gene toxR. J Bacteriol 1985 2.59
50 Staphylococcal enterotoxin A is encoded by phage. Science 1985 2.57
51 Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host. Proc Natl Acad Sci U S A 1995 2.57
52 Expression of ToxR, the transcriptional activator of the virulence factors in Vibrio cholerae, is modulated by the heat shock response. Proc Natl Acad Sci U S A 1990 2.56
53 Simple method for purifying choleragenoid, the natural toxoid of Vibrio cholerae. Infect Immun 1977 2.56
54 Confirmation of the Fur operator site by insertion of a synthetic oligonucleotide into an operon fusion plasmid. J Bacteriol 1988 2.49
55 Safety, immunogenicity, and efficacy of live attenuated Vibrio cholerae O139 vaccine prototype. Lancet 1995 2.43
56 Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I. Proc Natl Acad Sci U S A 1988 2.38
57 Morphological studies on fimbriae expressed by Vibrio cholerae 01. Microb Pathog 1988 2.33
58 In vivo covalent cross-linking of cellular actin by the Vibrio cholerae RTX toxin. EMBO J 2000 2.32
59 Characterization of defensin resistance phenotypes associated with mutations in the phoP virulence regulon of Salmonella typhimurium. Infect Immun 1990 2.25
60 In vivo genetic analysis of bacterial virulence. Annu Rev Microbiol 1999 2.23
61 Regulation of cholera toxin by temperature, pH, and osmolarity. Methods Enzymol 1994 2.23
62 Effect of a recA mutation on cholera toxin gene amplification and deletion events. J Bacteriol 1986 2.22
63 Use of genetic recombination as a reporter of gene expression. Proc Natl Acad Sci U S A 1994 2.21
64 Vibrio parahaemolyticus has a homolog of the Vibrio cholerae toxRS operon that mediates environmentally induced regulation of the thermostable direct hemolysin gene. J Bacteriol 1993 2.18
65 Vibrio cholerae tolC is required for bile resistance and colonization. Infect Immun 2001 2.17
66 Purification of cholera toxin and its subunits: new methods of preparation and the use of hypertoxinogenic mutants. Infect Immun 1978 2.16
67 In vivo transduction with shiga toxin 1-encoding phage. Infect Immun 1998 2.16
68 New model for analysis of mucosal immunity: intestinal secretion of specific monoclonal immunoglobulin A from hybridoma tumors protects against Vibrio cholerae infection. Infect Immun 1991 2.14
69 Cloning of the Vibrio cholerae recA gene and construction of a Vibrio cholerae recA mutant. J Bacteriol 1986 2.13
70 TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol 2000 2.11
71 Differential regulation of multiple flagellins in Vibrio cholerae. J Bacteriol 1998 2.09
72 Single amino acid substitutions in the N-terminus of Vibrio cholerae TcpA affect colonization, autoagglutination, and serum resistance. Mol Microbiol 1995 2.03
73 Analysis of clinical and environmental strains of nontoxigenic Vibrio cholerae for susceptibility to CTXPhi: molecular basis for origination of new strains with epidemic potential. Infect Immun 1998 1.99
74 Antibodies directed against the toxin-coregulated pilus isolated from Vibrio cholerae provide protection in the infant mouse experimental cholera model. J Infect Dis 1990 1.97
75 Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 2000 1.95
76 Constitutive sensory transduction mutations in the Bordetella pertussis bvgS gene. J Bacteriol 1992 1.91
77 Effects of changes in membrane sodium flux on virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A 1999 1.90
78 Cholera toxin: genetic analysis, regulation, and role in pathogenesis. Curr Top Microbiol Immunol 1985 1.89
79 Monoclonal immunoglobulin A prevents adherence and invasion of polarized epithelial cell monolayers by Salmonella typhimurium. Gastroenterology 1994 1.85
80 Induction of the lysogenic phage encoding cholera toxin in naturally occurring strains of toxigenic Vibrio cholerae O1 and O139. Infect Immun 1998 1.81
81 Vibrio cholerae O139 specific gene sequences. Lancet 1994 1.80
82 Characterization of Vibrio cholerae bacteriophage K139 and use of a novel mini-transposon to identify a phage-encoded virulence factor. Mol Microbiol 1995 1.80
83 Type 6 secretion dynamics within and between bacterial cells. Science 2012 1.79
84 Simultaneous prevention of glutamine synthesis and high-affinity transport attenuates Salmonella typhimurium virulence. Infect Immun 1997 1.77
85 Nucleotide sequence and spatiotemporal expression of the Vibrio cholerae vieSAB genes during infection. J Bacteriol 1998 1.72
86 ToxR regulates the production of lipoproteins and the expression of serum resistance in Vibrio cholerae. Proc Natl Acad Sci U S A 1991 1.72
87 A new assay for invasion of HeLa 229 cells by Bordetella pertussis: effects of inhibitors, phenotypic modulation, and genetic alterations. Infect Immun 1990 1.70
88 Expression of the Vibrio cholerae gene encoding aldehyde dehydrogenase is under control of ToxR, the cholera toxin transcriptional activator. J Bacteriol 1991 1.67
89 Replication and integration of a Vibrio cholerae cryptic plasmid linked to the CTX prophage. Mol Microbiol 1998 1.62
90 Acetylation (O-factor 5) affects the structural and immunological properties of Salmonella typhimurium lipopolysaccharide O antigen. Infect Immun 1995 1.52
91 Analysis of the roles of antilipopolysaccharide and anti-cholera toxin immunoglobulin A (IgA) antibodies in protection against Vibrio cholerae and cholera toxin by use of monoclonal IgA antibodies in vivo. Infect Immun 1993 1.52
92 Role of cholera toxin in enteric colonization by Vibrio cholerae O1 in rabbits. Infect Immun 1985 1.51
93 A vir-repressed gene of Bordetella pertussis is required for virulence. Infect Immun 1992 1.47
94 Regulation of cholera toxin production in Vibrio cholerae: genetic analysis of phenotypic instability in hypertoxinogenic mutants. J Bacteriol 1980 1.47
95 Genetic mapping of Vibrio cholerae enterotoxin structural genes. J Bacteriol 1984 1.45
96 Genetic mapping of toxin regulatory mutations in Vibrio cholerae. J Bacteriol 1979 1.45
97 Lipoprotein e(P4) is essential for hemin uptake by Haemophilus influenzae. J Exp Med 1996 1.43
98 Neither the Bvg- phase nor the vrg6 locus of Bordetella pertussis is required for respiratory infection in mice. Infect Immun 1998 1.41
99 Heterologous antigen expression in Vibrio cholerae vector strains. Infect Immun 1995 1.41
100 Molecular characterization of the oafA locus responsible for acetylation of Salmonella typhimurium O-antigen: oafA is a member of a family of integral membrane trans-acylases. J Bacteriol 1996 1.39
101 Immunogenicity of Vibrio cholerae O1 toxin-coregulated pili in experimental and clinical cholera. Infect Immun 1991 1.39
102 Association of protease activity in Vibrio cholerae vaccine strains with decreases in transcellular epithelial resistance of polarized T84 intestinal epithelial cells. Infect Immun 2000 1.36
103 Characterization of vir-activated TnphoA gene fusions in Bordetella pertussis. Infect Immun 1991 1.35
104 Evidence that modulation requires sequences downstream of the promoters of two vir-repressed genes of Bordetella pertussis. J Bacteriol 1990 1.35
105 In vivo expression technology for selection of bacterial genes specifically induced in host tissues. Methods Enzymol 1994 1.32
106 Vibrio cholerae-induced cellular responses of polarized T84 intestinal epithelial cells are dependent on production of cholera toxin and the RTX toxin. Infect Immun 2001 1.30
107 Lysogenic conversion of environmental Vibrio mimicus strains by CTXPhi. Infect Immun 1999 1.27
108 New attenuated derivatives of Vibrio cholerae. Res Microbiol 1991 1.26
109 Transposon-based approaches to identify essential bacterial genes. Trends Microbiol 2000 1.24
110 Analysis of membrane protein interaction: ToxR can dimerize the amino terminus of phage lambda repressor. Mol Microbiol 1994 1.22
111 Determinants of the immunogenicity of live virulent and mutant Vibrio cholerae O1 in rabbit intestine. Infect Immun 1987 1.20
112 Monoclonal immunoglobulin A antibodies directed against cholera toxin prevent the toxin-induced chloride secretory response and block toxin binding to intestinal epithelial cells in vitro. Infect Immun 1993 1.19
113 Enzymic activity of cholera toxin. I. New method of assay and the mechanism of ADP-ribosyl transfer. J Biol Chem 1979 1.18
114 Identification of multiple sigma54-dependent transcriptional activators in Vibrio cholerae. J Bacteriol 1998 1.16
115 Analysis of Vibrio cholerae ToxR function by construction of novel fusion proteins. Mol Microbiol 1995 1.14
116 ToxR proteins with substitutions in residues conserved with OmpR fail to activate transcription from the cholera toxin promoter. J Bacteriol 1992 1.13
117 rfb mutations in Vibrio cholerae do not affect surface production of toxin-coregulated pili but still inhibit intestinal colonization. Infect Immun 1999 1.12
118 Modulation of horizontal gene transfer in pathogenic bacteria by in vivo signals. Cell 1996 1.10
119 Determinants of immunogenicity and mechanisms of protection by virulent and mutant Vibrio cholerae O1 in rabbits. Infect Immun 1988 1.10
120 Repressor binding to a regulatory site in the DNA coding sequence is sufficient to confer transcriptional regulation of the vir-repressed genes (vrg genes) in Bordetella pertussis. J Bacteriol 1993 1.08
121 Measurement of transcriptional activity in pathogenic bacteria recovered directly from infected host tissue. Biotechniques 1994 1.03
122 Selection for bacterial genes that are specifically induced in host tissues: the hunt for virulence factors. Infect Agents Dis 1993 1.03
123 The ToxR protein of Vibrio cholerae forms homodimers and heterodimers. J Bacteriol 1996 1.02
124 Identification of a pilus colonization factor that is coordinately regulated with cholera toxin. Ann Sclavo Collana Monogr 1986 1.02
125 Structural analysis of the acfA and acfD genes of Vibrio cholerae: effects of DNA topology and transcriptional activators on expression. J Bacteriol 1992 1.00
126 Cloning and expression of the Salmonella enterotoxin gene. J Bacteriol 1987 0.98
127 Construction of a Vibrio cholerae vaccine candidate using transposon delivery and FLP recombinase-mediated excision. Infect Immun 2000 0.98
128 Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions. J Bacteriol 1993 0.96
129 Production and purification of cholera toxin. Methods Enzymol 1988 0.96
130 Isolation of peptide aptamers that inhibit intracellular processes. Proc Natl Acad Sci U S A 2000 0.93
131 ToxR co-operative interactions are not modulated by environmental conditions or periplasmic domain conformation. Mol Microbiol 1999 0.89
132 Salmonella vaccines with mutations in the phoP virulence regulon. Res Microbiol 1991 0.89
133 Diminished diarrheal response to Vibrio cholerae strains carrying the replicative form of the CTX(Phi) genome instead of CTX(Phi) lysogens in adult rabbits. Infect Immun 2001 0.88
134 Helicobacter pylori mutagenesis by mariner in vitro transposition. FEMS Immunol Med Microbiol 2001 0.84
135 Quartz crystal microbalance detection of Vibrio cholerae O139 serotype. J Immunol Methods 1995 0.84
136 Identification of a bacteriophage containing a silent staphylococcal variant enterotoxin gene (sezA+). Infect Immun 1990 0.81
137 Use of lambda phage S and R gene products in an inducible lysis system for Vibrio cholerae- and Salmonella enterica serovar typhimurium-based DNA vaccine delivery systems. Infect Immun 2000 0.80
138 Reversion of recombinant toxoids: mutations in diphtheria toxin that partially compensate for active-site deletions. Proc Natl Acad Sci U S A 1992 0.80
139 Morphological and physical characterization of the capsular layer of Vibrio cholerae O139. Arch Microbiol 1998 0.80
140 Identification and use of protective monoclonal IgA antibodies against viral and bacterial pathogens. Adv Exp Med Biol 1991 0.78
141 Strategies for the development of vaccines for typhoid fever, shigellosis, and cholera. Ann N Y Acad Sci 1989 0.78
142 Detection of NAD:arginine ADPribosyltransferases in animal tissues using 125I-labeled 1-(p-hydroxyphenyl) 2-guanidinoethane as ADPribose acceptor. Biochim Biophys Acta 1985 0.75