Importance of hydrogen-bonding interactions involving the side chain of Asp158 in the catalytic mechanism of papain.

Lecithin retinol acyltransferase is a founder member of a novel family of enzymes. Biochemistry (2003) 1.14

Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe. Biochem J (1993) 1.01

Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu. Biochem J (1993) 0.97

Ionization characteristics and chemical influences of aspartic acid residue 158 of papain and caricain determined by structure-related kinetic and computational techniques: multiple electrostatic modulators of active-centre chemistry. Biochem J (2000) 0.97

Roles of Ser101, Asp236, and His237 in catalysis of thioesterase II and of the C-terminal region of the enzyme in its interaction with fatty acid synthase. Proc Natl Acad Sci U S A (1993) 0.95

Evaluation of hydrogen-bonding and enantiomeric P2-S2 hydrophobic contacts in dynamic aspects of molecular recognition by papain. Biochem J (1992) 0.89

Is strong hydrogen bonding in the transition state enough to account for the observed rate acceleration in a mutant of papain? Proc Natl Acad Sci U S A (1997) 0.88

Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain. Biochem J (1994) 0.85

Challenging a paradigm: theoretical calculations of the protonation state of the Cys25-His159 catalytic diad in free papain. Proteins (2009) 0.85

Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group. Biochem J (1993) 0.84

Interaction of aspartic acid-104 and proline-287 with the active site of m-calpain. Biochem J (1996) 0.78

Conserved water-mediated H-bonding dynamics of catalytic His159 and Asp158: insight into a possible acid-base coupled mechanism in plant thiol protease. J Mol Model (2011) 0.75

A family of yeast expression vectors containing the phage f1 intergenic region. Gene (1987) 5.25

The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein. Cell (1989) 5.07

SSR alpha and associated calnexin are major calcium binding proteins of the endoplasmic reticulum membrane. J Biol Chem (1991) 4.48

The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. EMBO J (1992) 3.91

Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature (1993) 3.82

Signal transduction through homologs of the Ste20p and Ste7p protein kinases can trigger hyphal formation in the pathogenic fungus Candida albicans. Proc Natl Acad Sci U S A (1996) 3.50

Calnexin: a membrane-bound chaperone of the endoplasmic reticulum. Trends Biochem Sci (1994) 3.32

Signaling through adenylyl cyclase is essential for hyphal growth and virulence in the pathogenic fungus Candida albicans. Mol Biol Cell (2001) 2.99

Roles of the Candida albicans mitogen-activated protein kinase homolog, Cek1p, in hyphal development and systemic candidiasis. Infect Immun (1998) 2.98

Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin. Science (1995) 2.94

Concentration of MgATP2- and other ions in solution. Calculation of the true concentrations of species present in mixtures of associating ions. Biochem J (1976) 2.75

Alignment/phylogeny of the papain superfamily of cysteine proteases. J Mol Biol (1995) 2.61

Pheromone signalling and polarized morphogenesis in yeast. Curr Opin Genet Dev (1997) 2.59

gp25L/emp24/p24 protein family members of the cis-Golgi network bind both COP I and II coatomer. J Cell Biol (1998) 2.56

The kinetics of coupled enzyme reactions. Applications to the assay of glucokinase, with glucose 6-phosphate dehydrogenase as coupling enzyme. Biochem J (1974) 2.43

Virulence and hyphal formation of Candida albicans require the Ste20p-like protein kinase CaCla4p. Curr Biol (1997) 2.33

Ras links cellular morphogenesis to virulence by regulation of the MAP kinase and cAMP signalling pathways in the pathogenic fungus Candida albicans. Mol Microbiol (2001) 2.32

The Structure of calnexin, an ER chaperone involved in quality control of protein folding. Mol Cell (2001) 2.32

Enhanced catalysis of ribonuclease B folding by the interaction of calnexin or calreticulin with ERp57. J Biol Chem (1998) 2.27

Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. J Biol Chem (1995) 2.26

Overexpression of the STE4 gene leads to mating response in haploid Saccharomyces cerevisiae. Mol Cell Biol (1990) 2.21

Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase. EMBO J (1997) 2.20

Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the beta-galactosidase gene. J Virol (1990) 2.17

Proteomics characterization of abundant Golgi membrane proteins. J Biol Chem (2000) 2.13

Enhanced secretion from insect cells of a foreign protein fused to the honeybee melittin signal peptide. Gene (1991) 2.13

A role for myosin-I in actin assembly through interactions with Vrp1p, Bee1p, and the Arp2/3 complex. J Cell Biol (2000) 2.13

Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase. Anal Biochem (1986) 2.05

The terminal O-acetyltransferase involved in vindoline biosynthesis defines a new class of proteins responsible for coenzyme A-dependent acyl transfer. Plant J (1998) 2.00

Htm1p, a mannosidase-like protein, is involved in glycoprotein degradation in yeast. EMBO Rep (2001) 1.90

Association of the yeast pheromone response G protein beta gamma subunits with the MAP kinase scaffold Ste5p. Science (1995) 1.88

Kinetics of rat liver glucokinase. Co-operative interactions with glucose at physiologically significant concentrations. Biochem J (1976) 1.81

Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex. Mol Biol Cell (1999) 1.80

Yeast KEX1 gene encodes a putative protease with a carboxypeptidase B-like function involved in killer toxin and alpha-factor precursor processing. Cell (1987) 1.79

Dysthyroid optic neuropathy. Clinical profile and rationale for management. Arch Ophthalmol (1978) 1.77

Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases. Nature (1998) 1.77

Expression of the gene coding for the small subunit of ribulosebisphosphate carboxylase during differentiation of tobacco plant protoplasts. Eur J Biochem (1982) 1.76

Functional complementation of yeast ste6 by a mammalian multidrug resistance mdr gene. Science (1992) 1.74

Synthesis of human insulin gene. VIII. Construction of expression vectors for fused proinsulin production in Escherichia coli. Gene (1984) 1.64

Saccharomyces cerevisiae CNE1 encodes an endoplasmic reticulum (ER) membrane protein with sequence similarity to calnexin and calreticulin and functions as a constituent of the ER quality control apparatus. J Biol Chem (1995) 1.62

Role of the occluding loop in cathepsin B activity. J Biol Chem (1997) 1.61

Conformation-independent binding of monoglucosylated ribonuclease B to calnexin. Cell (1997) 1.58

Kinetic evidence for a 'mnemonical' mechanism for rat liver glucokinase. Biochem J (1977) 1.54

Structure of the cel-3 gene from Fibrobacter succinogenes S85 and characteristics of the encoded gene product, endoglucanase 3. J Bacteriol (1989) 1.54

Purification and characterization of a chloride-stimulated cellobiosidase from Bacteroides succinogenes S85. J Bacteriol (1988) 1.52

The nature of experimental error in enzyme kinetic measurments. Biochem J (1975) 1.51

Association of calnexin with mutant peripheral myelin protein-22 ex vivo: a basis for "gain-of-function" ER diseases. Proc Natl Acad Sci U S A (2002) 1.50

Functional expression of human cathepsin S in Saccharomyces cerevisiae. Purification and characterization of the recombinant enzyme. J Biol Chem (1993) 1.49

Structure of rat procathepsin B: model for inhibition of cysteine protease activity by the proregion. Structure (1996) 1.47

Dominant negative selection of heterologous genes: isolation of Candida albicans genes that interfere with Saccharomyces cerevisiae mating factor-induced cell cycle arrest. Proc Natl Acad Sci U S A (1992) 1.46

Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling. EMBO J (1992) 1.45

Derepressed hyphal growth and reduced virulence in a VH1 family-related protein phosphatase mutant of the human pathogen Candida albicans. Mol Biol Cell (1997) 1.45

Potential for manipulation of the rumen fermentation through the use of recombinant DNA techniques. J Anim Sci (1986) 1.44

Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1. Mol Gen Genet (1993) 1.42

Structural and functional roles of asparagine 175 in the cysteine protease papain. J Biol Chem (1995) 1.40

The phosphorylation site for Ste20p-like protein kinases is essential for the function of myosin-I in yeast. J Biol Chem (1997) 1.40

Recombination of mitochondrial drug-resistance factors in Saccharomyces cerevisiae. Biochem Biophys Res Commun (1968) 1.40

Contribution of the glutamine 19 side chain to transition-state stabilization in the oxyanion hole of papain. Biochemistry (1991) 1.40

Characteristics of the endoglucanase encoded by a cel gene from Bacteroides succinogenes expressed in Escherichia coli. Appl Environ Microbiol (1987) 1.40

Ca2+ regulation of interactions between endoplasmic reticulum chaperones. J Biol Chem (1999) 1.39

Mutations in the active site of penicillin-binding protein PBP2x from Streptococcus pneumoniae. Role in the specificity for beta-lactam antibiotics. J Biol Chem (1999) 1.34

Activation of myosin-I by members of the Ste20p protein kinase family. J Biol Chem (1996) 1.33

Constitutive activation of the Saccharomyces cerevisiae mating response pathway by a MAP kinase kinase from Candida albicans. Mol Gen Genet (1995) 1.32

A protein engineering study of the role of aspartate 158 in the catalytic mechanism of papain. Biochemistry (1990) 1.32

ERp57 and PDI: multifunctional protein disulfide isomerases with similar domain architectures but differing substrate-partner associations. Biochem Cell Biol (2006) 1.30

Structure and transcriptional control of the Saccharomyces cerevisiae POX1 gene encoding acyl-coenzyme A oxidase. Gene (1990) 1.29

The purification in high yield and characterization of rat hepatic glucokinase. Biochem J (1976) 1.29

Cell cycle- and Cln2p-Cdc28p-dependent phosphorylation of the yeast Ste20p protein kinase. J Biol Chem (1998) 1.28

Cloning and sequencing of the preprotoxin-coding region of the yeast M1 double-stranded RNA. EMBO J (1984) 1.28

Control of expression, glycosylation, and secretion of HIV-1 gp120 by homologous and heterologous signal sequences. Virology (1994) 1.27

Identification of a structural determinant for resistance to beta-lactam antibiotics in Gram-positive bacteria. Proc Natl Acad Sci U S A (1998) 1.27

Potency and selectivity of the cathepsin L propeptide as an inhibitor of cysteine proteases. Biochemistry (1996) 1.26

The endoplasmic reticulum: integration of protein folding, quality control, signaling and degradation. Curr Opin Struct Biol (2001) 1.25

Screening and identification of a gene, PSE-1, that affects protein secretion in Saccharomyces cerevisiae. J Cell Sci (1992) 1.25

Crystal structure of a papain-E-64 complex. Biochemistry (1989) 1.24

Coordinated amino acid changes in homologous protein families. Protein Eng (1988) 1.24

RNA polymerases stall and/or prematurely terminate nearby both early and late promoters on polyomavirus DNA. J Mol Biol (1988) 1.19

Candida albicans killing by RAW 264.7 mouse macrophage cells: effects of Candida genotype, infection ratios, and gamma interferon treatment. Infect Immun (2002) 1.18