PubRank

Richard J Simpson

Author PubWeight™ 149.25‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Exosomes: extracellular organelles important in intercellular communication. J Proteomics 2010 6.47
2 Overview of the HUPO Plasma Proteome Project: results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database. Proteomics 2005 5.42
3 Human Proteinpedia enables sharing of human protein data. Nat Biotechnol 2008 4.21
4 ExoCarta: A compendium of exosomal proteins and RNA. Proteomics 2009 3.72
5 Guidelines for the next 10 years of proteomics. Proteomics 2006 3.61
6 An evaluation, comparison, and accurate benchmarking of several publicly available MS/MS search algorithms: sensitivity and specificity analysis. Proteomics 2005 3.51
7 ExoCarta 2012: database of exosomal proteins, RNA and lipids. Nucleic Acids Res 2011 3.28
8 Vesiclepedia: a compendium for extracellular vesicles with continuous community annotation. PLoS Biol 2012 3.18
9 Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods 2012 2.46
10 HtrA2 promotes cell death through its serine protease activity and its ability to antagonize inhibitor of apoptosis proteins. J Biol Chem 2001 2.33
11 Mining a tandem mass spectrometry database to determine the trends and global factors influencing peptide fragmentation. Anal Chem 2003 2.28
12 Proteomics analysis of A33 immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature. Mol Cell Proteomics 2009 2.08
13 Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma. Proteomics 2013 1.93
14 An aspartyl protease directs malaria effector proteins to the host cell. Nature 2010 1.92
15 Soil microorganisms mediating phosphorus availability update on microbial phosphorus. Plant Physiol 2011 1.71
16 Role of the Plasmodium export element in trafficking parasite proteins to the infected erythrocyte. Traffic 2008 1.64
17 Restoration of full-length adenomatous polyposis coli (APC) protein in a colon cancer cell line enhances cell adhesion. J Cell Sci 2003 1.51
18 Aerobic fitness is associated with lower proportions of senescent blood T-cells in man. Brain Behav Immun 2011 1.49
19 Proteome profiling of exosomes derived from human primary and metastatic colorectal cancer cells reveal differential expression of key metastatic factors and signal transduction components. Proteomics 2013 1.46
20 Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Mol Cell Proteomics 2012 1.44
21 Plasmin activates the lymphangiogenic growth factors VEGF-C and VEGF-D. J Exp Med 2003 1.35
22 Plasma Proteome Database as a resource for proteomics research: 2014 update. Nucleic Acids Res 2013 1.34
23 Identification of a PRMT5-dependent repressor complex linked to silencing of human fetal globin gene expression. Blood 2010 1.28
24 SOCS-6 binds to insulin receptor substrate 4, and mice lacking the SOCS-6 gene exhibit mild growth retardation. Mol Cell Biol 2002 1.28
25 Enzymic, phylogenetic, and structural characterization of the unusual papain-like protease domain of Plasmodium falciparum SERA5. J Biol Chem 2003 1.17
26 Characterization of an antagonist interleukin-6 dimer by stable isotope labeling, cross-linking, and mass spectrometry. J Biol Chem 2002 1.14
27 A protocol for exosome isolation and characterization: evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods. Methods Mol Biol 2015 1.09
28 Comprehensive lipidome profiling of isogenic primary and metastatic colon adenocarcinoma cell lines. Anal Chem 2012 1.08
29 Human microglial cells synthesize albumin in brain. PLoS One 2008 1.07
30 Transgenic barley (Hordeum vulgare L.) expressing the wheat aluminium resistance gene (TaALMT1) shows enhanced phosphorus nutrition and grain production when grown on an acid soil. Plant Biotechnol J 2009 1.06
31 Carotid plaque hemorrhage on magnetic resonance imaging and recurrent cerebrovascular events. J Am Coll Cardiol 2014 1.06
32 Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation. J Biol Chem 2006 1.05
33 Expression of a fungal phytase gene in Nicotiana tabacum improves phosphorus nutrition of plants grown in amended soils. Plant Biotechnol J 2005 1.04
34 Oncogenic H-ras reprograms Madin-Darby canine kidney (MDCK) cell-derived exosomal proteins following epithelial-mesenchymal transition. Mol Cell Proteomics 2013 1.04
35 A proteome strategy for fractionating proteins and peptides using continuous free-flow electrophoresis coupled off-line to reversed-phase high-performance liquid chromatography. Anal Chem 2004 1.00
36 Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. Electrophoresis 2008 1.00
37 Secretome-based proteomic profiling of Ras-transformed MDCK cells reveals extracellular modulators of epithelial-mesenchymal transition. J Proteome Res 2009 0.99
38 Insulin and oleate promote translocation of inosine-5' monophosphate dehydrogenase to lipid bodies. Traffic 2004 0.99
39 Effect of soil acidity, soil strength and macropores on root growth and morphology of perennial grass species differing in acid-soil resistance. Plant Cell Environ 2010 0.97
40 PHLDA1 expression marks the putative epithelial stem cells and contributes to intestinal tumorigenesis. Cancer Res 2011 0.97
41 Olig2-induced neural stem cell differentiation involves downregulation of Wnt signaling and induction of Dickkopf-1 expression. PLoS One 2008 0.96
42 Application of 2-D free-flow electrophoresis/RP-HPLC for proteomic analysis of human plasma depleted of multi high-abundance proteins. Proteomics 2005 0.96
43 Extracellular vesicle isolation and characterization: toward clinical application. J Clin Invest 2016 0.95
44 Proteomics profiling of Madin-Darby canine kidney plasma membranes reveals Wnt-5a involvement during oncogenic H-Ras/TGF-beta-mediated epithelial-mesenchymal transition. Mol Cell Proteomics 2010 0.94
45 Extracellular remodelling during oncogenic Ras-induced epithelial-mesenchymal transition facilitates MDCK cell migration. J Proteome Res 2010 0.94
46 Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam Principles). Mol Cell Proteomics 2011 0.94
47 Stabilization of proteins for storage. Cold Spring Harb Protoc 2010 0.94
48 Contribution of cells undergoing epithelial-mesenchymal transition to the tumour microenvironment. J Proteomics 2012 0.94
49 Nanoparticle interactions with zinc and iron: implications for toxicology and inflammation. Toxicol Appl Pharmacol 2007 0.93
50 Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam Principles). J Proteome Res 2011 0.91
51 Body fluid proteomics: Prospects for biomarker discovery. Proteomics Clin Appl 2007 0.91
52 A centrifugal ultrafiltration strategy for isolating the low-molecular weight (<or=25K) component of human plasma proteome. J Proteomics 2009 0.90
53 Is a gene-centric human proteome project the best way for proteomics to serve biology? Proteomics 2010 0.90
54 Identifying mutated proteins secreted by colon cancer cell lines using mass spectrometry. J Proteomics 2012 0.90
55 Towards understanding epithelial-mesenchymal transition: a proteomics perspective. Biochim Biophys Acta 2009 0.90
56 Isolation of extracellular membranous vesicles for proteomic analysis. Methods Mol Biol 2009 0.89
57 The phosphoprotein StarD10 is overexpressed in breast cancer and cooperates with ErbB receptors in cellular transformation. Cancer Res 2004 0.88
58 Comparison of human platelet membrane-cytoskeletal proteins with the plasma proteome: Towards understanding the platelet-plasma nexus. Proteomics Clin Appl 2007 0.88
59 Proteomic profiling of secretome and adherent plasma membranes from distinct mammary epithelial cell subpopulations. Proteomics 2011 0.88
60 Mutation detection using mass spectrometric separation of tiny oligonucleotide fragments. Genome Res 2002 0.88
61 Proteomic comparison of 3D and 2D glioma models reveals increased HLA-E expression in 3D models is associated with resistance to NK cell-mediated cytotoxicity. J Proteome Res 2014 0.87
62 Detection of cadherin-17 in human colon cancer LIM1215 cell secretome and tumour xenograft-derived interstitial fluid and plasma. Biochim Biophys Acta 2013 0.87
63 Gesicles: Microvesicle "cookies" for transient information transfer between cells. Mol Ther 2011 0.87
64 Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles). Proteomics 2011 0.87
65 Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4. Electrophoresis 2012 0.87
66 Triton X-114 phase separation in the isolation and purification of mouse liver microsomal membrane proteins. Methods 2011 0.87
67 In situ phosphorylation of immobilized receptors on biosensor surfaces: application to E-cadherin/beta-catenin interactions. Anal Biochem 2006 0.87
68 CHOMPER: a bioinformatic tool for rapid validation of tandem mass spectrometry search results associated with high-throughput proteomic strategies. Proteomics 2002 0.86
69 Selective identification and quantitative analysis of methionine containing peptides by charge derivatization and tandem mass spectrometry. J Am Soc Mass Spectrom 2005 0.85
70 Secretome-based proteomics reveals sulindac-modulated proteins released from colon cancer cells. Proteomics Clin Appl 2009 0.85
71 A fluorescent microsphere-based method for assay of multiple analytes in plasma. Methods Mol Biol 2011 0.85
72 Disruption of cultured cells by nitrogen cavitation. Cold Spring Harb Protoc 2010 0.84
73 Fitness level impacts salivary antimicrobial protein responses to a single bout of cycling exercise. Eur J Appl Physiol 2015 0.84
74 Staining proteins in gels with silver nitrate. CSH Protoc 2007 0.84
75 Acute aerobic exercise in humans increases cytokine expression in CD27(-) but not CD27(+) CD8(+) T-cells. Brain Behav Immun 2012 0.83
76 Miniaturized asymmetrical flow field-flow fractionation: application to biological vesicles. J Sep Sci 2007 0.83
77 The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard. Proteomics 2010 0.82
78 Mixed lineage kinase 2 interacts with clathrin and influences clathrin-coated vesicle trafficking. J Biol Chem 2002 0.82
79 Proteomic profiling of the epithelial-mesenchymal transition using 2D DIGE. Methods Mol Biol 2012 0.82
80 Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides. J Proteome Res 2007 0.82
81 Stem cell markers: insights from membrane proteomics? Proteomics 2008 0.82
82 Tandem application of cationic colloidal silica and Triton X-114 for plasma membrane protein isolation and purification: towards developing an MDCK protein database. Proteomics 2011 0.81
83 NK cells and exercise: implications for cancer immunotherapy and survivorship. Discov Med 2015 0.81
84 The microvesicle component of HIV-1 inocula modulates dendritic cell infection and maturation and enhances adhesion to and activation of T lymphocytes. PLoS Pathog 2013 0.81
85 Liquid-based free-flow electrophoresis-reversed-phase HPLC: a proteomic tool. Nat Methods 2005 0.81
86 Total lymphocyte CD8 expression is not a reliable marker of cytotoxic T-cell populations in human peripheral blood following an acute bout of high-intensity exercise. Brain Behav Immun 2007 0.81
87 NK-cells have an impaired response to acute exercise and a lower expression of the inhibitory receptors KLRG1 and CD158a in humans with latent cytomegalovirus infection. Brain Behav Immun 2011 0.81
88 Estimation of Free Thiols and Disulfide Bonds Using Ellman's Reagent. CSH Protoc 2008 0.80
89 Defining a longitudinal survival model to examine forced treadmill running as a countermeasure for diet-induced weight gain. Lab Anim 2010 0.80
90 Immune responses to exercising in a cold environment. Wilderness Environ Med 2011 0.80
91 International blood collection and storage: clinical use of blood products. J Proteomics 2009 0.80
92 The effects of age and viral serology on γδ T-cell numbers and exercise responsiveness in humans. Cell Immunol 2013 0.79
93 Low-molecular weight plasma proteome analysis using centrifugal ultrafiltration. Methods Mol Biol 2011 0.79
94 The impact of acute strenuous exercise on TLR2, TLR4 and HLA.DR expression on human blood monocytes induced by autologous serum. Eur J Appl Physiol 2010 0.79
95 Can exercise-related improvements in immunity influence cancer prevention and prognosis in the elderly? Maturitas 2013 0.79
96 Homogenization of mammalian tissue. Cold Spring Harb Protoc 2010 0.79
97 The tap-fill method for dry packing columns of rigid solids. CSH Protoc 2006 0.79
98 A proteomic approach for unraveling the oncogenic H-Ras protein networks in NIH/3T3 mouse embryonic fibroblast cells. Proteomics 2006 0.79
99 The role of WDR5 in silencing human fetal globin gene expression. Haematologica 2012 0.78
100 CTAB-PAGE. Cold Spring Harb Protoc 2010 0.78
101 Isolation and characterisation of a 13.8-kDa bacteriolytic enzyme from house dust mite extracts: homology with prokaryotic proteins suggests that the enzyme could be bacterially derived. FEMS Immunol Med Microbiol 2002 0.78
102 Analysis of Ras-induced oncogenic transformation of NIH-3T3 cells using differential-display 2-DE proteomics. Electrophoresis 2007 0.78
103 Identification of a Wnt-induced protein complex by affinity proteomics using an antibody that recognizes a sub-population of β-catenin. Biochim Biophys Acta 2012 0.78
104 Zinc/Imidazole procedure for visualization of proteins in gels by negative staining. CSH Protoc 2007 0.78
105 Latent cytomegalovirus infection and innate immune function following a 75 km cycling time trial. Eur J Appl Physiol 2013 0.77
106 Chromosome 7-centric analysis of proteomics data from a panel of human colon carcinoma cell lines. J Proteome Res 2012 0.77
107 Staining proteins in gels with coomassie blue. CSH Protoc 2007 0.77
108 Global protein profiling reveals anti-EGFR monoclonal antibody 806-modulated proteins in A431 tumor xenografts. Growth Factors 2013 0.77
109 Rapid coomassie blue staining of protein gels. Cold Spring Harb Protoc 2010 0.77
110 Solubilisation of the armadillo-repeat protein β-catenin using a zwitterionic detergent allows resolution of phosphorylated forms by 2DE. Electrophoresis 2012 0.77
111 [From genome to proteome--aim of human proteomics]. Seikagaku 2004 0.77
112 Quantifying protein by bicinchoninic Acid. CSH Protoc 2008 0.77
113 Enrichment of human platelet membranes for proteomic analysis. Methods Mol Biol 2009 0.77
114 Purification of basolateral integral membrane proteins by cationic colloidal silica-based apical membrane subtraction. Methods Mol Biol 2009 0.77
115 Sulindac modulates secreted protein expression from LIM1215 colon carcinoma cells prior to apoptosis. Biochim Biophys Acta 2013 0.76
116 A protocol for the preparation of cryoprecipitate and cryodepleted plasma. Methods Mol Biol 2011 0.76
117 Bulk precipitation of proteins by ammonium sulfate. CSH Protoc 2006 0.76
118 An updated secretome. Biochim Biophys Acta 2013 0.76
119 Proteomic analysis of colorectal cancer: prefractionation strategies using two-dimensional free-flow electrophoresis. Comp Funct Genomics 2005 0.76
120 CMV amplifies T-cell redeployment to acute exercise independently of HSV-1 serostatus. Med Sci Sports Exerc 2014 0.76
121 SYPRO Orange fluorescent staining of protein gels. Cold Spring Harb Protoc 2010 0.76
122 Crystal structure of the Leishmania major MIX protein: a scaffold protein that mediates protein-protein interactions. Protein Sci 2011 0.75
123 The impact of 6-month training preparation for an Ironman triathlon on the proportions of naïve, memory and senescent T cells in resting blood. Eur J Appl Physiol 2011 0.75
124 Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles). Proteomics Clin Appl 2011 0.75
125 Colon tumour secretopeptidome: insights into endogenous proteolytic cleavage events in the colon tumour microenvironment. Biochim Biophys Acta 2013 0.75
126 Antigen-specific CD8+ T-cells Are Mobilized With Exercise Via β2-adrenergic Receptor Signaling Pathways: Implications For Immunotherapy?: 2664 Board #187 June 3, 9: 30 AM - 11: 00 AM. Med Sci Sports Exerc 2016 0.75
127 Task imposed on prisons is impossible. BMJ 2003 0.75
128 Cleavage at met-x bonds by cyanogen bromide. CSH Protoc 2007 0.75
129 Preparation of extracts from yeast. Cold Spring Harb Protoc 2011 0.75
130 The Effects Of Exercise Intensity And Duration On NK-cell Cytotoxicity And Proliferative Responses To IL-15: 337 Board #174 June 1, 9: 30 AM - 11: 00 AM. Med Sci Sports Exerc 2016 0.75
131 SDS-PAGE of Proteins. CSH Protoc 2006 0.75
132 Breast cancer protein StarD10 identified by three-dimensional separation using free-flow electrophoresis, reversed-phase high-performance liquid chromatography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Electrophoresis 2005 0.75
133 Precipitation of proteins by organic solvents. CSH Protoc 2006 0.75
134 Concentrating acrylamide gel spots. CSH Protoc 2006 0.75
135 Proteomic analysis of colorectal cancer: discovering novel biomarkers. Expert Rev Proteomics 2005 0.75
136 Reduction and s-carboxymethylation of proteins: microscale method. CSH Protoc 2007 0.75
137 Fabricating protein arrays. CSH Protoc 2007 0.75
138 Enhancing The Generation Of Adenovirus-Specific T Cells With Exercise For Immunotherapy: 336 Board #173 June 1, 9: 30 AM - 11: 00 AM. Med Sci Sports Exerc 2016 0.75
139 Chromatography using a crystalline powder form of hydroxyapatite. CSH Protoc 2006 0.75
140 Pouring linear gradient gels with a gradient former. Cold Spring Harb Protoc 2010 0.75
141 Modification of lysyl side chains using succinic anhydride. CSH Protoc 2006 0.75
142 Protein separation on ceramic hydroxyapatite using high-performance liquid chromatography. CSH Protoc 2006 0.75
143 Large-scale extraction of recombinant proteins from bacteria. Cold Spring Harb Protoc 2010 0.75
144 Precipitation of proteins by polyethylenimine. CSH Protoc 2006 0.75
145 Reliability of the Kinetics of British Army Foot-Drill in Untrained Personnel. J Strength Cond Res 2016 0.75
146 Cleavage at Tryptophan by o-Iodosobenzoic Acid. CSH Protoc 2007 0.75
147 Cleavage of asn-gly bonds by hydroxylamine. CSH Protoc 2007 0.75
148 The Effects of British Army Footwear on Ground Reaction Force and Temporal Parameters of British Army Foot-Drill. J Strength Cond Res 2017 0.75
149 Precipitation of proteins by polyethylene glycol. CSH Protoc 2006 0.75
150 Reduction and s-carboxymethylation of proteins: large-scale method. CSH Protoc 2007 0.75
151 Correction for Zhang et al., The conserved SOCS box motif in suppressors of cytokine signaling binds to elongins B and C and may couple bound proteins to proteasomal degradation. Proc Natl Acad Sci U S A 2015 0.75
152 Assays with protein arrays. CSH Protoc 2007 0.75
153 Nomenclature on proteases, proteinases, and peptidases. CSH Protoc 2007 0.75
154 In Situ SDS-PAGE Peptide-Mapping Procedure (Cleveland Method). CSH Protoc 2007 0.75
155 SDS-PAGE Peptide-Mapping Procedure (Cleveland Method). CSH Protoc 2007 0.75
156 Separation of proteins using hydrophobic interaction chromatography. CSH Protoc 2006 0.75
157 Correction: Molecular profiling of cetuximab and bevacizumab treatment of colorectal tumours reveals perturbations in metabolic and hypoxic response pathways. Oncotarget 2016 0.75
158 Redeployment Of Immune Cell Subsets Across Multiple Exercise Intensities In The Context Of Cytomegalovirus Infection: 2916 June 3 2: 00 PM - 2: 15 PM. Med Sci Sports Exerc 2016 0.75
159 Performic Acid oxidation of proteins. CSH Protoc 2007 0.75
160 In-gel s-pyridylethylation of gel-resolved proteins: individual spot method. CSH Protoc 2008 0.75
161 Fragmentation of protein using trypsin. CSH Protoc 2006 0.75
162 Modification of lysyl side chains using citraconic anhydride. CSH Protoc 2006 0.75
163 Fluorescent Staining of Proteins with SYPRO Ruby. CSH Protoc 2006 0.75
164 Electrophoresis of Peptides (Tricine-SDS-PAGE). CSH Protoc 2006 0.75
165 Preparation of platelet concentrates. Methods Mol Biol 2011 0.75
166 Integrated device for the measurement of systemic and local oxygen transport during physical exercise. Conf Proc IEEE Eng Med Biol Soc 2012 0.75
167 Packing ceramic hydroxyapatite columns for high-performance liquid chromatography. CSH Protoc 2006 0.75
168 A Single Bout Of Exercise Enhances The Ex Vivo Manufacture Of Viral-specific T-cells: 330 Board #167 June 1, 9: 30 AM - 11: 00 AM. Med Sci Sports Exerc 2016 0.75
169 Small-scale extraction of recombinant proteins from bacteria. Cold Spring Harb Protoc 2010 0.75
170 In-gel s-pyridylethylation of gel-resolved proteins: whole gel method. CSH Protoc 2008 0.75
171 Can A Single Exercise Bout Increase The Yield Of Hematopoietic Stem Cells From Peripheral Blood?: 335 Board #172 June 1, 9: 30 AM - 11: 00 AM. Med Sci Sports Exerc 2016 0.75
172 Fractional precipitation of proteins by ammonium sulfate. CSH Protoc 2006 0.75
173 Solubilization of Escherichia coli recombinant proteins from inclusion bodies. Cold Spring Harb Protoc 2010 0.75