Published in Biochim Biophys Acta on July 13, 2010
Molecular basis for the inability of an oxygen atom donor ligand to replace the natural sulfur donor heme axial ligand in cytochrome P450 catalysis. Spectroscopic characterization of the Cys436Ser CYP2B4 mutant. Arch Biochem Biophys (2010) 0.91
A single-site mutation (F429H) converts the enzyme CYP 2B4 into a heme oxygenase: a QM/MM study. J Am Chem Soc (2012) 0.87
Role of the Proximal Cysteine Hydrogen Bonding Interaction in Cytochrome P450 2B4 Studied by Cryoreduction, Electron Paramagnetic Resonance, and Electron-Nuclear Double Resonance Spectroscopy. Biochemistry (2016) 0.77
Structural and functional characterization of a cytochrome P450 2B4 F429H mutant with an axial thiolate-histidine hydrogen bond. Biochemistry (2014) 0.77
Spectroscopic studies of the cytochrome P450 reaction mechanisms. Biochim Biophys Acta (2017) 0.75
THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE. J Biol Chem (1964) 24.57
Heme-Containing Oxygenases. Chem Rev (1996) 5.82
The catalytic pathway of cytochrome p450cam at atomic resolution. Science (2000) 5.42
Common and uncommon cytochrome P450 reactions related to metabolism and chemical toxicity. Chem Res Toxicol (2001) 4.78
Mechanism of oxidation reactions catalyzed by cytochrome p450 enzymes. Chem Rev (2004) 4.25
Structure of mammalian cytochrome P450 2B4 complexed with 4-(4-chlorophenyl)imidazole at 1.9-A resolution: insight into the range of P450 conformations and the coordination of redox partner binding. J Biol Chem (2004) 2.66
Probing structure-function relations in heme-containing oxygenases and peroxidases. Science (1988) 2.63
Hydroxylation of camphor by reduced oxy-cytochrome P450cam: mechanistic implications of EPR and ENDOR studies of catalytic intermediates in native and mutant enzymes. J Am Chem Soc (2001) 2.48
Crystallographic study on the dioxygen complex of wild-type and mutant cytochrome P450cam. Implications for the dioxygen activation mechanism. J Biol Chem (2005) 1.77
Uncoupling of the cytochrome P-450cam monooxygenase reaction by a single mutation, threonine-252 to alanine or valine: possible role of the hydroxy amino acid in oxygen activation. Proc Natl Acad Sci U S A (1989) 1.75
Autooxidation and hydroxylation reactions of oxygenated cytochrome P-450cam. J Biol Chem (1976) 1.44
Peroxo-iron and oxenoid-iron species as alternative oxygenating agents in cytochrome P450-catalyzed reactions: switching by threonine-302 to alanine mutagenesis of cytochrome P450 2B4. Proc Natl Acad Sci U S A (1996) 1.39
Oxidizing species in the mechanism of cytochrome P450. Nat Prod Rep (2002) 1.34
Molecular recognition in cytochrome P-450: mechanism for the control of uncoupling reactions. Biochemistry (1993) 1.32
Roles of the proximal heme thiolate ligand in cytochrome p450(cam). J Am Chem Soc (2001) 1.19
Oxygenated cytochrome P-450 and its possible role in enzymic hydroxylation. Biochem Biophys Res Commun (1971) 1.17
Substrate modulation of the properties and reactivity of the oxy-ferrous and hydroperoxo-ferric intermediates of cytochrome P450cam as shown by cryoreduction-EPR/ENDOR spectroscopy. J Am Chem Soc (2005) 1.15
A new spectral intermediate associated with cytochrome P-450 function in liver microsomes. Biochem Biophys Res Commun (1971) 1.14
Theoretical study of the ligand-CYP2B4 complexes: effect of structure on binding free energies and heme spin state. Proteins (2004) 1.13
Magnetic circular dichroism studies of myoglobin complexes. Correlations with heme spin state and axial ligation. J Am Chem Soc (1976) 1.12
Characterization of the microsomal cytochrome P450 2B4 O2 activation intermediates by cryoreduction and electron paramagnetic resonance. Biochemistry (2008) 1.09
Roles of the proximal hydrogen bonding network in cytochrome P450cam-catalyzed oxygenation. J Am Chem Soc (2002) 1.06
The diverse spectroscopic properties of ferrous cytochrome P-450-CAM ligand complexes. J Biol Chem (1983) 1.00
Oxygen activation and electron transfer in flavocytochrome P450 BM3. J Am Chem Soc (2003) 0.97
Determination of the rate of reduction of oxyferrous cytochrome P450 2B4 by 5-deazariboflavin adenine dinucleotide T491V cytochrome P450 reductase. Biochemistry (2003) 0.96
Kinetics of oxidation of benzphetamine by compounds I of cytochrome P450 2B4 and its mutants. J Am Chem Soc (2009) 0.94
Properties of the oxygenated form of liver microsomal cytochrome P-450. J Biol Chem (1983) 0.90
The influence of substrate on the spectral properties of oxyferrous wild-type and T252A cytochrome P450-CAM. Arch Biochem Biophys (2005) 0.89
Kinetic isotope effects implicate two electrophilic oxidants in cytochrome p450-catalyzed hydroxylations. J Am Chem Soc (2003) 0.87
Magnetic circular dichroism studies of cytochrome P-450cam. Characterization of axial ligands of ferric and ferrous low-spin complexes. Biochim Biophys Acta (1981) 0.82
Subzero-temperature stabilization and spectroscopic characterization of homogeneous oxyferrous complexes of the cytochrome P450 BM3 (CYP102) oxygenase domain and holoenzyme. Biochem Biophys Res Commun (2005) 0.81
Oxygenated cytochrome P-450cam: evidence against axial histidine ligation of iron. FEBS Lett (1978) 0.79
Observation of structural variation and spin state conversion of cytochrome P450 CYP2B4 on binding of sterically different substrates. Biochem Biophys Res Commun (1996) 0.76
miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes. Nucleic Acids Res (2008) 3.19
Oxoiron(IV) in chloroperoxidase compound II is basic: implications for P450 chemistry. Science (2004) 2.38
Structure and function of an NADPH-cytochrome P450 oxidoreductase in an open conformation capable of reducing cytochrome P450. J Biol Chem (2009) 1.54
Solid-state NMR reveals structural and dynamical properties of a membrane-anchored electron-carrier protein, cytochrome b5. J Am Chem Soc (2007) 1.53
The cytochromes P450 and b5 and their reductases--promising targets for structural studies by advanced solid-state NMR spectroscopy. Biochim Biophys Acta (2007) 1.47
Characterization of the periplasmic heme-binding protein shut from the heme uptake system of Shigella dysenteriae. Biochemistry (2005) 1.31
Reaction of ferric cytochrome P450cam with peracids: kinetic characterization of intermediates on the reaction pathway. J Biol Chem (2005) 1.30
Cytochrome b5 increases the rate of product formation by cytochrome P450 2B4 and competes with cytochrome P450 reductase for a binding site on cytochrome P450 2B4. J Biol Chem (2007) 1.28
Deamidation: Differentiation of aspartyl from isoaspartyl products in peptides by electron capture dissociation. Protein Sci (2005) 1.23
Neutral thiol as a proximal ligand to ferrous heme iron: implications for heme proteins that lose cysteine thiolate ligation on reduction. Proc Natl Acad Sci U S A (2003) 1.20
Bicelle-enabled structural studies on a membrane-associated cytochrome B5 by solid-state MAS NMR spectroscopy. Angew Chem Int Ed Engl (2008) 1.17
The interaction of microsomal cytochrome P450 2B4 with its redox partners, cytochrome P450 reductase and cytochrome b(5). Arch Biochem Biophys (2010) 1.16
Conformational changes of NADPH-cytochrome P450 oxidoreductase are essential for catalysis and cofactor binding. J Biol Chem (2011) 1.15
Substrate modulation of the properties and reactivity of the oxy-ferrous and hydroperoxo-ferric intermediates of cytochrome P450cam as shown by cryoreduction-EPR/ENDOR spectroscopy. J Am Chem Soc (2005) 1.15
Epoxidation of olefins by hydroperoxo-ferric cytochrome P450. J Am Chem Soc (2003) 1.14
EPR and ENDOR characterization of intermediates in the cryoreduced oxy-nitric oxide synthase heme domain with bound L-arginine or N(G)-hydroxyarginine. Biochemistry (2002) 1.14
Role of cytochrome b5 in catalysis by cytochrome P450 2B4. Biochem Biophys Res Commun (2005) 1.13
Theoretical study of the ligand-CYP2B4 complexes: effect of structure on binding free energies and heme spin state. Proteins (2004) 1.13
A model of the membrane-bound cytochrome b5-cytochrome P450 complex from NMR and mutagenesis data. J Biol Chem (2013) 1.12
Characterization of the microsomal cytochrome P450 2B4 O2 activation intermediates by cryoreduction and electron paramagnetic resonance. Biochemistry (2008) 1.09
Cytochrome b5 inhibits electron transfer from NADPH-cytochrome P450 reductase to ferric cytochrome P450 2B4. J Biol Chem (2007) 1.08
Rapid freeze-quench ENDOR study of chloroperoxidase compound I: the site of the radical. J Am Chem Soc (2006) 1.08
A nanomolar-potency small molecule inhibitor of regulator of G-protein signaling proteins. Biochemistry (2011) 1.07
Structural analysis of mammalian cytochrome P450 2B4 covalently bound to the mechanism-based inactivator tert-butylphenylacetylene: insight into partial enzymatic activity. Biochemistry (2011) 1.05
EpoK, a cytochrome P450 involved in biosynthesis of the anticancer agents epothilones A and B. Substrate-mediated rescue of a P450 enzyme. Biochemistry (2004) 1.04
Probing the spontaneous membrane insertion of a tail-anchored membrane protein by sum frequency generation spectroscopy. J Am Chem Soc (2010) 1.03
Proton-evolved local-field solid-state NMR studies of cytochrome b5 embedded in bicelles, revealing both structural and dynamical information. J Am Chem Soc (2010) 1.03
The role of the length and sequence of the linker domain of cytochrome b5 in stimulating cytochrome P450 2B4 catalysis. J Biol Chem (2004) 1.03
Resonance Raman studies of cytochrome P450 2B4 in its interactions with substrates and redox partners. Biochemistry (2008) 1.01
Increased Foxp3(+) Treg cell activity reduces dendritic cell co-stimulatory molecule expression in aged mice. Mech Ageing Dev (2007) 1.00
Spectroscopic investigations of intermediates in the reaction of cytochrome P450(BM3)-F87G with surrogate oxygen atom donors. J Inorg Biochem (2006) 0.98
Characterization of heme ligation properties of Rv0203, a secreted heme binding protein involved in Mycobacterium tuberculosis heme uptake. Biochemistry (2012) 0.96
Determination of the rate of reduction of oxyferrous cytochrome P450 2B4 by 5-deazariboflavin adenine dinucleotide T491V cytochrome P450 reductase. Biochemistry (2003) 0.96
Hydroperoxoferric heme intermediate as a second electrophilic oxidant in cytochrome P450-catalyzed reactions. J Biol Inorg Chem (2004) 0.95
INEPT-based separated-local-field NMR spectroscopy: a unique approach to elucidate side-chain dynamics of membrane-associated proteins. J Am Chem Soc (2010) 0.95
Substitutions of the "bridging" aspartate 178 result in profound changes in the reactivity of the Rieske center of phthalate dioxygenase. Biochemistry (2006) 0.94
Kinetics of oxidation of benzphetamine by compounds I of cytochrome P450 2B4 and its mutants. J Am Chem Soc (2009) 0.94
Probing the transmembrane structure and topology of microsomal cytochrome-p450 by solid-state NMR on temperature-resistant bicelles. Sci Rep (2013) 0.93
Direct observation of a novel perturbed oxyferrous catalytic intermediate during reduced putidaredoxin-initiated turnover of cytochrome P-450-CAM: probing the effector role of putidaredoxin in catalysis. J Biol Chem (2005) 0.93
Replacement of tyrosine residues by phenylalanine in cytochrome P450cam alters the formation of Cpd II-like species in reactions with artificial oxidants. J Biol Inorg Chem (2008) 0.93
Dynamic interaction between membrane-bound full-length cytochrome P450 and cytochrome b5 observed by solid-state NMR spectroscopy. Sci Rep (2013) 0.92
Probing the oxyferrous and catalytically active ferryl states of Amphitrite ornata dehaloperoxidase by cryoreduction and EPR/ENDOR spectroscopy. Detection of compound I. J Am Chem Soc (2010) 0.92
Characterization of SiaA, a streptococcal heme-binding protein associated with a heme ABC transport system. Biochemistry (2008) 0.92
Molecular basis for the inability of an oxygen atom donor ligand to replace the natural sulfur donor heme axial ligand in cytochrome P450 catalysis. Spectroscopic characterization of the Cys436Ser CYP2B4 mutant. Arch Biochem Biophys (2010) 0.91
Rapid kinetics investigations of peracid oxidation of ferric cytochrome P450cam: nature and possible function of compound ES. J Inorg Biochem (2006) 0.91
EPR and ENDOR studies of cryoreduced compounds II of peroxidases and myoglobin. Proton-coupled electron transfer and protonation status of ferryl hemes. Biochemistry (2008) 0.90
Mechanism-based inactivation of CYP2B1 and its F-helix mutant by two tert-butyl acetylenic compounds: covalent modification of prosthetic heme versus apoprotein. J Pharmacol Exp Ther (2009) 0.90
Cryoreduction EPR and 13C, 19F ENDOR study of substrate-bound substates and solvent kinetic isotope effects in the catalytic cycle of cytochrome P450cam and its T252A mutant. Dalton Trans (2005) 0.90
Structures of thiolate- and carboxylate-ligated ferric H93G myoglobin: models for cytochrome P450 and for oxyanion-bound heme proteins. Biochemistry (2006) 0.90
The influence of substrate on the spectral properties of oxyferrous wild-type and T252A cytochrome P450-CAM. Arch Biochem Biophys (2005) 0.89
Peroxynitrite inactivation of human cytochrome P450s 2B6 and 2E1: heme modification and site-specific nitrotyrosine formation. Chem Res Toxicol (2007) 0.89
Replacement of the axial histidine heme ligand with cysteine in nitrophorin 1: spectroscopic and crystallographic characterization. J Biol Inorg Chem (2008) 0.89
Oxygen activation by cytochrome P450 monooxygenase. Photosynth Res (2008) 0.89
Defining the structural consequences of mechanism-based inactivation of mammalian cytochrome P450 2B4 using resonance Raman spectroscopy. J Am Chem Soc (2010) 0.88
Heme binding to the Mammalian circadian clock protein period 2 is nonspecific. Biochemistry (2010) 0.88
Covalent modification of Thr302 in cytochrome P450 2B1 by the mechanism-based inactivator 4-tert-butylphenylacetylene. J Pharmacol Exp Ther (2010) 0.87
Kinetic isotope effects in hydroxylation reactions effected by cytochrome P450 compounds I implicate multiple electrophilic oxidants for P450-catalyzed oxidations. Biochemistry (2009) 0.87
A single-site mutation (F429H) converts the enzyme CYP 2B4 into a heme oxygenase: a QM/MM study. J Am Chem Soc (2012) 0.87
Uncovering the role of hydrophobic residues in cytochrome P450-cytochrome P450 reductase interactions. Biochemistry (2011) 0.87
The mechanism of oxidative halophenol dehalogenation by Amphitrite ornata dehaloperoxidase is initiated by H2O2 binding and involves two consecutive one-electron steps: role of ferryl intermediates. Biochemistry (2009) 0.86
Cytochrome-P450-cytochrome-b5 interaction in a membrane environment changes 15N chemical shift anisotropy tensors. J Phys Chem B (2013) 0.86
The use of deuterated camphor as a substrate in (1)H ENDOR studies of hydroxylation by cryoreduced oxy P450cam provides new evidence of the involvement of compound I. Biochemistry (2013) 0.85
High levels of expression of the iron-sulfur proteins phthalate dioxygenase and phthalate dioxygenase reductase in Escherichia coli. Protein Expr Purif (2006) 0.85
Sensitizer-linked substrates and ligands: ruthenium probes of cytochrome P450 structure and mechanism. Methods Enzymol (2002) 0.85
Spectroscopic characterization of the ferric states of Amphitrite ornata dehaloperoxidase and Notomastus lobatus chloroperoxidase: His-ligated peroxidases with globin-like proximal and distal properties. J Inorg Biochem (2006) 0.85
Mutation of tyrosine 190 to alanine eliminates the inactivation of cytochrome P450 2B1 by peroxynitrite. Chem Res Toxicol (2003) 0.85
Evaluation of electron-withdrawing group effects on heme binding in designed proteins: implications for heme a in cytochrome c oxidase. Inorg Chem (2006) 0.85
Mapping the binding interface of the cytochrome b5-cytochrome c complex by nuclear magnetic resonance. Biochemistry (2003) 0.84
Redox-dependent stability, protonation, and reactivity of cysteine-bound heme proteins. Proc Natl Acad Sci U S A (2014) 0.84
A novel intermediate in the reaction of seleno CYP119 with m-chloroperbenzoic acid. Biochemistry (2011) 0.84
Metabolic activation of mifepristone [RU486; 17beta-hydroxy-11beta-(4-dimethylaminophenyl)-17alpha-(1-propynyl)-estra-4,9-dien-3-one] by mammalian cytochromes P450 and the mechanism-based inactivation of human CYP2B6. J Pharmacol Exp Ther (2009) 0.84
Threonine-205 in the F helix of p450 2B1 contributes to androgen 16 beta-hydroxylation activity and mechanism-based inactivation. J Pharmacol Exp Ther (2003) 0.84