Published in Curr Opin Chem Biol on April 15, 2011
Deep mutational scanning: a new style of protein science. Nat Methods (2014) 2.05
A fundamental protein property, thermodynamic stability, revealed solely from large-scale measurements of protein function. Proc Natl Acad Sci U S A (2012) 1.39
Construction of a stability landscape of the CH3 domain of human IgG1 by combining directed evolution with high throughput sequencing. J Mol Biol (2012) 1.06
Stabilizing proteins from sequence statistics: the interplay of conservation and correlation in triosephosphate isomerase stability. J Mol Biol (2012) 1.03
Protein stability: computation, sequence statistics, and new experimental methods. Curr Opin Struct Biol (2015) 0.83
Consensus design of a NOD receptor leucine rich repeat domain with binding affinity for a muramyl dipeptide, a bacterial cell wall fragment. Protein Sci (2014) 0.81
Structural and dynamic properties that govern the stability of an engineered fibronectin type III domain. Protein Eng Des Sel (2015) 0.80
Consensus protein design. Protein Eng Des Sel (2016) 0.80
Ligands for glaucoma-associated myocilin discovered by a generic binding assay. ACS Chem Biol (2013) 0.78
Optically induced thermal gradients for protein characterization in nanolitre-scale samples in microfluidic devices. Sci Rep (2013) 0.76
Protein evolution analysis of S-hydroxynitrile lyase by complete sequence design utilizing the INTMSAlign software. Sci Rep (2015) 0.75
High-throughput identification of protein mutant stability computed from a double mutant fitness landscape. Protein Sci (2015) 0.75
Improved drug-like properties of therapeutic proteins by directed evolution. Protein Eng Des Sel (2012) 0.75
Dominant forces in protein folding. Biochemistry (1990) 15.34
Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell (2002) 10.81
Evolutionarily conserved pathways of energetic connectivity in protein families. Science (1999) 9.12
The structure and function of G-protein-coupled receptors. Nature (2009) 8.08
Rapid protein-folding assay using green fluorescent protein. Nat Biotechnol (1999) 6.98
Thermofluor-based high-throughput stability optimization of proteins for structural studies. Anal Biochem (2006) 5.05
High-density miniaturized thermal shift assays as a general strategy for drug discovery. J Biomol Screen (2001) 4.75
Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination. Proc Natl Acad Sci U S A (2006) 4.45
Protein sectors: evolutionary units of three-dimensional structure. Cell (2009) 3.76
Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nat Biotechnol (2004) 3.68
Evolutionary information for specifying a protein fold. Nature (2005) 3.23
Design of stable alpha-helical arrays from an idealized TPR motif. Structure (2003) 3.17
Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies. Proc Natl Acad Sci U S A (2002) 3.15
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nat Biotechnol (2010) 3.14
Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments. Proc Natl Acad Sci U S A (1998) 2.96
Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. J Am Chem Soc (2005) 2.95
Microscale fluorescent thermal stability assay for membrane proteins. Structure (2008) 2.95
Consensus-derived structural determinants of the ankyrin repeat motif. Proc Natl Acad Sci U S A (2002) 2.87
Surface sites for engineering allosteric control in proteins. Science (2008) 2.82
Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling. Proc Natl Acad Sci U S A (2003) 2.43
Hydrogen bonding, hydrophobicity, packing, and protein folding. Annu Rev Biophys Biomol Struct (1993) 2.21
Pulse proteolysis: a simple method for quantitative determination of protein stability and ligand binding. Nat Methods (2005) 2.18
Optimizing protein stability in vivo. Mol Cell (2009) 2.15
Directed evolution of a thermostable esterase. Proc Natl Acad Sci U S A (1998) 2.12
Designed to be stable: crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci U S A (2003) 1.98
Allosteric determinants in guanine nucleotide-binding proteins. Proc Natl Acad Sci U S A (2003) 1.91
Quantitative protein stability measurement in vivo. Nat Struct Biol (2001) 1.85
High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering. J Am Chem Soc (2009) 1.85
Sequence statistics reliably predict stabilizing mutations in a protein domain. J Mol Biol (1994) 1.77
Quantification of dynamic protein complexes using Renilla luciferase fragment complementation applied to protein kinase A activities in vivo. Proc Natl Acad Sci U S A (2007) 1.73
A quantitative, high-throughput screen for protein stability. Proc Natl Acad Sci U S A (2000) 1.52
Directed evolution of proteins for heterologous expression and stability. Curr Opin Struct Biol (2005) 1.43
An interdomain sector mediating allostery in Hsp70 molecular chaperones. Mol Syst Biol (2010) 1.42
Sequence variation in ligand binding sites in proteins. BMC Bioinformatics (2005) 1.37
Rapid and simple protein-stability screens: application to membrane proteins. Acta Crystallogr D Biol Crystallogr (2006) 1.36
The consensus concept for thermostability engineering of proteins: further proof of concept. Protein Eng (2002) 1.36
Protein fabrication automation. Protein Sci (2007) 1.33
Screening for ligands using a generic and high-throughput light-scattering-based assay. J Biomol Screen (2006) 1.31
Thermodynamic analysis of protein stability and ligand binding using a chemical modification- and mass spectrometry-based strategy. Anal Chem (2008) 1.27
Stability engineering of scFvs for the development of bispecific and multivalent antibodies. Protein Eng Des Sel (2010) 1.27
The consensus concept for thermostability engineering of proteins. Biochim Biophys Acta (2000) 1.26
High throughput methods of assessing protein stability and aggregation. Mol Biosyst (2008) 1.23
Rapid mapping of protein structure, interactions, and ligand binding by misincorporation proton-alkyl exchange. J Biol Chem (2002) 1.21
High throughput thermostability screening of monoclonal antibody formulations. J Pharm Sci (2010) 1.19
Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes. Biochemistry (2010) 1.19
Energetics-based protein profiling on a proteomic scale: identification of proteins resistant to proteolysis. J Mol Biol (2007) 1.15
Beyond consensus: statistical free energies reveal hidden interactions in the design of a TPR motif. J Mol Biol (2004) 1.14
LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment. Biophys J (2010) 1.13
Experimental library screening demonstrates the successful application of computational protein design to large structural ensembles. Proc Natl Acad Sci U S A (2010) 1.04
Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity. Proc Natl Acad Sci U S A (2010) 1.02
High-throughput screening for enhanced protein stability. Curr Opin Biotechnol (2006) 1.02
Application of high-throughput isothermal denaturation to assess protein stability and screen for ligands. J Biomol Screen (2008) 1.00
Combinatorial approaches to protein stability and structure. Eur J Biochem (2004) 0.99
High-throughput measurement of protein stability in microtiter plates. Biotechnol Bioeng (2005) 0.98
Formulation development of therapeutic monoclonal antibodies using high-throughput fluorescence and static light scattering techniques: role of conformational and colloidal stability. J Pharm Sci (2010) 0.96
Instrumentation for automated determination of protein stability. Anal Biochem (1995) 0.96
A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity. Proteins (2011) 0.96
Rapid expression and purification of 100 nmol quantities of active protein using cell-free protein synthesis. Biotechnol Prog (2004) 0.95
Designing thermostable proteins: ancestral mutants of 3-isopropylmalate dehydrogenase designed by using a phylogenetic tree. J Mol Biol (2005) 0.94
High-precision, high-throughput stability determinations facilitated by robotics and a semiautomated titrating fluorometer. Biochemistry (2003) 0.92
Consensus protein design without phylogenetic bias. J Mol Biol (2010) 0.91
Automated, high-throughput platform for protein solubility screening using a split-GFP system. J Struct Funct Genomics (2008) 0.89
Intrabody construction and expression III: engineering hyperstable V(H) domains. Protein Sci (1999) 0.89
Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale. Protein Sci (2010) 0.87
High-throughput analysis of the protein sequence-stability landscape using a quantitative yeast surface two-hybrid system and fragment reconstitution. J Mol Biol (2008) 0.87
Free sulfhydryl measurement as an indicator of antibody stability. Anal Biochem (2008) 0.85
A high-throughput assay of membrane protein stability. Mol Membr Biol (2008) 0.83
Directed evolution of serum paraoxonase PON3 by family shuffling and ancestor/consensus mutagenesis, and its biochemical characterization. Biochemistry (2009) 0.83
Quantitative determination of protein stability and ligand binding using a green fluorescent protein reporter system. Mol Biosyst (2010) 0.81
In vivo protein stabilization based on fragment complementation and a split GFP system. Proc Natl Acad Sci U S A (2010) 0.79
High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire. Nat Biotechnol (2013) 2.81
High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering. J Am Chem Soc (2009) 1.85
Molecular deconvolution of the monoclonal antibodies that comprise the polyclonal serum response. Proc Natl Acad Sci U S A (2013) 1.26
Direct single-molecule observation of a protein living in two opposed native structures. Proc Natl Acad Sci U S A (2009) 1.07
Cysteine-free Rop: a four-helix bundle core mutant has wild-type stability and structure but dramatically different unfolding kinetics. Protein Sci (2010) 0.92
Proteomic identification of monoclonal antibodies from serum. Anal Chem (2014) 0.84
Simplifying protein expression with ligation-free, traceless and tag-switching plasmids. Protein Expr Purif (2012) 0.80
Synthetic approach to stop-codon scanning mutagenesis. J Am Chem Soc (2011) 0.75