Published in Biochemistry on June 26, 1979
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A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo. Proc Natl Acad Sci U S A (1981) 3.14
Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro. Proc Natl Acad Sci U S A (1982) 2.78
Stereospecificity of nucleases towards phosphorothioate-substituted RNA: stereochemistry of transcription by T7 RNA polymerase. Nucleic Acids Res (1987) 1.82
Synthesis and resistance to enzymic hydrolysis of stereochemically-defined phosphonate and thiophosphate analogues of P1,P4-bis(5'-adenosyl) tetraphosphate. Nucleic Acids Res (1987) 1.13
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Intramolecular and intermolecular uridylylation by poliovirus RNA-dependent RNA polymerase. J Virol (2006) 1.03
The cleavage of DNA at phosphorothioate internucleotidic linkages by DNA gyrase. Nucleic Acids Res (1992) 1.03
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Enzymatic techniques for the isolation of random single-base substitutions in vitro at high frequency. Proc Natl Acad Sci U S A (1984) 0.97
Acetate kinase: a triple-displacement enzyme. Proc Natl Acad Sci U S A (1980) 0.97
Synthesis and separation of diastereomers of deoxynucleoside 5'-O-(1-thio)triphosphates. Nucleic Acids Res (1983) 0.92
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Stereodifferentiation--the effect of P chirality of oligo(nucleoside phosphorothioates) on the activity of bacterial RNase H. Nucleic Acids Res (1995) 0.82
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The stereochemical course of hydrolysis catalysed by snake venom 5'-nucleotide phosphodiesterase. Biochem J (1981) 0.80
Mung bean (Phaseolus aureus) nuclease. A mechanistic investigation of the DNA-cleavage reaction using a dinucleoside phosphorothioate. Biochem J (1987) 0.80
Rat liver nucleotide pyrophosphatase/phosphodiesterase is an efficient adenylyl transferase. Biochem J (2000) 0.77
Solid support synthesis of all-Rp-oligo(ribonucleoside phosphorothioate)s. Nucleic Acids Res (1996) 0.75
Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc Natl Acad Sci U S A (1991) 5.94
Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science (1989) 5.73
Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli. Biochemistry (1987) 4.29
Kinetic mechanism of DNA polymerase I (Klenow). Biochemistry (1987) 3.93
Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase. Science (1992) 3.81
A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo. Proc Natl Acad Sci U S A (1981) 3.14
Kinetic mechanism of DNA polymerase I (Klenow fragment): identification of a second conformational change and evaluation of the internal equilibrium constant. Biochemistry (1991) 3.07
At the crossroads of chemistry and immunology: catalytic antibodies. Science (1991) 3.06
Kinetic mechanism whereby DNA polymerase I (Klenow) replicates DNA with high fidelity. Biochemistry (1988) 2.86
Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro. Proc Natl Acad Sci U S A (1982) 2.78
Backbone dynamics in dihydrofolate reductase complexes: role of loop flexibility in the catalytic mechanism. Biochemistry (2001) 2.77
Human immunodeficiency virus type 1 reverse transcriptase: spatial and temporal relationship between the polymerase and RNase H activities. Proc Natl Acad Sci U S A (1992) 2.64
Evidence for a functional role of the dynamics of glycine-121 of Escherichia coli dihydrofolate reductase obtained from kinetic analysis of a site-directed mutant. Biochemistry (1997) 2.61
Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. Proc Natl Acad Sci U S A (1991) 2.42
Production of cyclic peptides and proteins in vivo. Proc Natl Acad Sci U S A (1999) 2.42
Kinetic characterization of the polymerase and exonuclease activities of the gene 43 protein of bacteriophage T4. Biochemistry (1992) 2.41
Chemical basis for enzyme catalysis. Biochemistry (2000) 2.09
Recombinant HIV-1 nucleocapsid protein accelerates HIV-1 reverse transcriptase catalyzed DNA strand transfer reactions and modulates RNase H activity. Biochemistry (1994) 2.08
Metallo-beta-lactamase: structure and mechanism. Curr Opin Chem Biol (1999) 2.06
Dynamics of a flexible loop in dihydrofolate reductase from Escherichia coli and its implication for catalysis. Biochemistry (1994) 2.04
Purification and characterization of human immunodeficiency virus type 1 reverse transcriptase. Methods Enzymol (1995) 2.01
Side chains involved in catalysis of the polymerase reaction of DNA polymerase I from Escherichia coli. J Biol Chem (1992) 1.97
Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific cDNA library. Proc Natl Acad Sci U S A (1989) 1.96
On the mechanism of the metallo-beta-lactamase from Bacteroides fragilis. Biochemistry (1999) 1.94
A mutant of DNA polymerase I (Klenow fragment) with reduced fidelity. Biochemistry (1991) 1.87
Dynamics of the dihydrofolate reductase-folate complex: catalytic sites and regions known to undergo conformational change exhibit diverse dynamical features. Biochemistry (1995) 1.86
Peptide synthesis catalyzed by an antibody containing a binding site for variable amino acids. Science (1994) 1.85
A combinatorial approach to hybrid enzymes independent of DNA homology. Nat Biotechnol (1999) 1.79
Elementary steps in the DNA polymerase I reaction pathway. Biochemistry (1983) 1.73
The nucleotide analog 2-aminopurine as a spectroscopic probe of nucleotide incorporation by the Klenow fragment of Escherichia coli polymerase I and bacteriophage T4 DNA polymerase. Biochemistry (1995) 1.69
Solvation, reorganization energy, and biological catalysis. J Biol Chem (1998) 1.62
Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. Proc Natl Acad Sci U S A (1993) 1.57
Characterization of the enzyme complex involving the folate-requiring enzymes of de novo purine biosynthesis. Biochemistry (1980) 1.48
Rate-limiting steps in the DNA polymerase I reaction pathway. Biochemistry (1985) 1.46
Combinatorial protein engineering by incremental truncation. Proc Natl Acad Sci U S A (1999) 1.46
Characterization of the metal-binding sites of the beta-lactamase from Bacteroides fragilis. Biochemistry (1996) 1.45
Stretching exercises--flexibility in dihydrofolate reductase catalysis. Chem Biol (1998) 1.43
DNA substrate structural requirements for the exonuclease and polymerase activities of procaryotic and phage DNA polymerases. Biochemistry (1989) 1.43
Protein-DNA cross-linking demonstrates stepwise ATP-dependent assembly of T4 DNA polymerase and its accessory proteins on the primer-template. Cell (1991) 1.42
Stereochemical course of the 3'----5'-exonuclease activity of DNA polymerase I. Biochemistry (1984) 1.40
Functional role of a mobile loop of Escherichia coli dihydrofolate reductase in transition-state stabilization. Biochemistry (1992) 1.39
Interaction of Escherichia coli DNA polymerase I with azidoDNA and fluorescent DNA probes: identification of protein-DNA contacts. Biochemistry (1990) 1.38
Accessory proteins function as matchmakers in the assembly of the T4 DNA polymerase holoenzyme. Curr Biol (1995) 1.37
Interloop contacts modulate ligand cycling during catalysis by Escherichia coli dihydrofolate reductase. Biochemistry (2001) 1.36
Cloning and characterization of a new purine biosynthetic enzyme: a non-folate glycinamide ribonucleotide transformylase from E. coli. Biochemistry (1994) 1.33
A fluorescence-based assay for monitoring helicase activity. Proc Natl Acad Sci U S A (1994) 1.33
A stereospecific cyclization catalyzed by an antibody. Science (1987) 1.31
Mechanism of action of fructose 1,6-bisphosphatase. Adv Enzymol Relat Areas Mol Biol (1982) 1.30
Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides. Nucleic Acids Res (2001) 1.28
On the mechanism of renaturation of complementary DNA strands by the recA protein of Escherichia coli. Proc Natl Acad Sci U S A (1985) 1.28
Glu-111 is required for activation of the DNA cleavage center of EcoRI endonuclease. J Biol Chem (1989) 1.27
Metalloantibodies. Science (1990) 1.27
The carboxyl terminus of the bacteriophage T4 DNA polymerase is required for holoenzyme complex formation. Proc Natl Acad Sci U S A (1996) 1.26
Bacteriophage T4 Dda helicase translocates in a unidirectional fashion on single-stranded DNA. J Biol Chem (1995) 1.26
A perspective on biological catalysis. Nat Struct Biol (1996) 1.26
Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase. Biochemistry (1990) 1.26
Deletion of a highly motional residue affects formation of the Michaelis complex for Escherichia coli dihydrofolate reductase. Biochemistry (1998) 1.25
Strength of an interloop hydrogen bond determines the kinetic pathway in catalysis by Escherichia coli dihydrofolate reductase. Biochemistry (1998) 1.24
Purification of a complex catalyzing folate cofactor synthesis and transformylation in de novo purine biosynthesis. J Biol Chem (1980) 1.23
Minimal kinetic mechanism for misincorporation by DNA polymerase I (Klenow fragment). Biochemistry (1992) 1.23
Effect of single amino acid replacements on the folding and stability of dihydrofolate reductase from Escherichia coli. Biochemistry (1987) 1.22
Sliding clamp of the bacteriophage T4 polymerase has open and closed subunit interfaces in solution. Biochemistry (1999) 1.22
Interaction of the recA protein of Escherichia coli with single-stranded DNA. J Biol Chem (1985) 1.21
Evaluation of the importance of hydrophobic interactions in drug binding to dihydrofolate reductase. J Med Chem (1988) 1.21
Antibody remodeling: a general solution to the design of a metal-coordination site in an antibody binding pocket. Proc Natl Acad Sci U S A (1990) 1.20
Induction of an antibody that catalyzes the hydrolysis of an amide bond. Science (1988) 1.19
Mutations in HIV reverse transcriptase which alter RNase H activity and decrease strand transfer efficiency are suppressed by HIV nucleocapsid protein. Proc Natl Acad Sci U S A (1997) 1.19
NMR characterization of the metallo-beta-lactamase from Bacteroides fragilis and its interaction with a tight-binding inhibitor: role of an active-site loop. Biochemistry (1999) 1.19
Catalytic reactions of phosphoglucose isomerase with cyclic forms of glucose 6-phosphate and fructose 6-phosphate. J Biol Chem (1973) 1.19
Creating a dynamic picture of the sliding clamp during T4 DNA polymerase holoenzyme assembly by using fluorescence resonance energy transfer. Proc Natl Acad Sci U S A (2001) 1.18
Creating multiple-crossover DNA libraries independent of sequence identity. Proc Natl Acad Sci U S A (2001) 1.18
Truncating alpha-helix E' of p66 human immunodeficiency virus reverse transcriptase modulates RNase H function and impairs DNA strand transfer. J Biol Chem (1995) 1.17
Stoichiometry and DNA unwinding by the bacteriophage T4 41:59 helicase. J Biol Chem (1996) 1.17
A zinc ribbon protein in DNA replication: primer synthesis and macromolecular interactions by the bacteriophage T4 primase. Biochemistry (2001) 1.17
Inactivation of DNA polymerase I (Klenow fragment) by adenosine 2',3'-epoxide 5'-triphosphate: evidence for the formation of a tight-binding inhibitor. Biochemistry (1989) 1.15
Tracking sliding clamp opening and closing during bacteriophage T4 DNA polymerase holoenzyme assembly. Biochemistry (2000) 1.15
Synthesis and application of derivatizable oligonucleotides. Nucleic Acids Res (1987) 1.14
Crystal structure of a bifunctional transformylase and cyclohydrolase enzyme in purine biosynthesis. Nat Struct Biol (2001) 1.14
Nonadditivity of mutational effects at the folate binding site of Escherichia coli dihydrofolate reductase. Biochemistry (1994) 1.14
Purification, characterization, and kinetic studies of a soluble Bacteroides fragilis metallo-beta-lactamase that provides multiple antibiotic resistance. J Biol Chem (1998) 1.14
Crystal structure of glycinamide ribonucleotide transformylase from Escherichia coli at 3.0 A resolution. A target enzyme for chemotherapy. J Mol Biol (1992) 1.13
The function of amino acid residues contacting the nicotinamide ring of NADPH in dihydrofolate reductase from Escherichia coli. Biochemistry (1991) 1.13
Towards structure-based drug design: crystal structure of a multisubstrate adduct complex of glycinamide ribonucleotide transformylase at 1.96 A resolution. J Mol Biol (1995) 1.13
Predicting crossover generation in DNA shuffling. Proc Natl Acad Sci U S A (2001) 1.12
Evolution of protein function by domain swapping. Adv Protein Chem (2000) 1.11
A cell cycle-regulated adenine DNA methyltransferase from Caulobacter crescentus processively methylates GANTC sites on hemimethylated DNA. Proc Natl Acad Sci U S A (1998) 1.11
A novel combined chemical-enzymatic synthesis of cross-linked DNA using a nucleoside triphosphate analogue. Biochemistry (1991) 1.10
Probing the functional role of phenylalanine-31 of Escherichia coli dihydrofolate reductase by site-directed mutagenesis. Biochemistry (1987) 1.10
Hybrid enzymes: manipulating enzyme design. Trends Biotechnol (1998) 1.09
A rapid screen of active site mutants in glycinamide ribonucleotide transformylase. Biochemistry (1996) 1.09
Molecular basis for nonadditive mutational effects in Escherichia coli dihydrofolate reductase. Biochemistry (1995) 1.08
Assembly of an active enzyme by the linkage of two protein modules. Proc Natl Acad Sci U S A (1997) 1.08
Rapid assembly of the bacteriophage T4 core replication complex on a linear primer/template construct. Proc Natl Acad Sci U S A (1993) 1.08
Mechanism of DNA polymerase I: exonuclease/polymerase activity switch and DNA sequence dependence of pyrophosphorolysis and misincorporation reactions. Proc Natl Acad Sci U S A (1986) 1.07
Structural requirements for the biosynthesis of backbone cyclic peptide libraries. Chem Biol (2001) 1.07
Divalent cation modulation of the ribonuclease functions of human immunodeficiency virus reverse transcriptase. Biochemistry (1995) 1.07
Interaction of DNA with the Klenow fragment of DNA polymerase I studied by time-resolved fluorescence spectroscopy. Biochemistry (1991) 1.07
A kinetic study of wild-type and mutant dihydrofolate reductases from Lactobacillus casei. Biochemistry (1989) 1.06
Characterization of bacteriophage T4-coordinated leading- and lagging-strand synthesis on a minicircle substrate. Proc Natl Acad Sci U S A (2000) 1.06