Direct sampling of cystic fibrosis lungs indicates that DNA-based analyses of upper-airway specimens can misrepresent lung microbiota.

PubWeight™: 2.22‹?› | Rank: Top 2%

🔗 View Article (PMC 3427132)

Published in Proc Natl Acad Sci U S A on August 07, 2012

Authors

Amanda F Goddard1, Benjamin J Staudinger, Scot E Dowd, Amruta Joshi-Datar, Randall D Wolcott, Moira L Aitken, Corinne L Fligner, Pradeep K Singh

Author Affiliations

1: Department of Pediatrics, University of Washington School of Medicine, Seattle, WA 98195, USA.

Articles citing this

Regional Isolation Drives Bacterial Diversification within Cystic Fibrosis Lungs. Cell Host Microbe (2015) 2.98

Type VI secretion system effectors: poisons with a purpose. Nat Rev Microbiol (2014) 2.43

Lung microbiota and bacterial abundance in patients with bronchiectasis when clinically stable and during exacerbation. Am J Respir Crit Care Med (2013) 2.08

Biogeochemical forces shape the composition and physiology of polymicrobial communities in the cystic fibrosis lung. MBio (2014) 1.74

Widespread colonization of the lung by Tropheryma whipplei in HIV infection. Am J Respir Crit Care Med (2013) 1.66

The role of the bacterial microbiome in lung disease. Expert Rev Respir Med (2013) 1.62

The role of the microbiome in exacerbations of chronic lung diseases. Lancet (2014) 1.55

Iron supplementation does not worsen respiratory health or alter the sputum microbiome in cystic fibrosis. J Cyst Fibros (2013) 1.53

Inflammation and airway microbiota during cystic fibrosis pulmonary exacerbations. PLoS One (2013) 1.43

The development of lower respiratory tract microbiome in mice. Microbiome (2017) 1.39

Breath gas metabolites and bacterial metagenomes from cystic fibrosis airways indicate active pH neutral 2,3-butanedione fermentation. ISME J (2014) 1.38

The Microbiome and the Respiratory Tract. Annu Rev Physiol (2015) 1.35

Rapid Detection of Emerging Pathogens and Loss of Microbial Diversity Associated with Severe Lung Disease in Cystic Fibrosis. J Clin Microbiol (2015) 1.29

Application of a neutral community model to assess structuring of the human lung microbiome. MBio (2015) 1.25

Spatial Variation in the Healthy Human Lung Microbiome and the Adapted Island Model of Lung Biogeography. Ann Am Thorac Soc (2015) 1.19

The cystic fibrosis airway microbiome. Cold Spring Harb Perspect Med (2013) 1.14

Clinical insights from metagenomic analysis of sputum samples from patients with cystic fibrosis. J Clin Microbiol (2013) 1.10

Mechanistic model of Rothia mucilaginosa adaptation toward persistence in the CF lung, based on a genome reconstructed from metagenomic data. PLoS One (2013) 1.10

Microbial communities in the respiratory tract of patients with interstitial lung disease. Thorax (2013) 1.08

The upper respiratory tract as a microbial source for pulmonary infections in cystic fibrosis. Parallels from island biogeography. Am J Respir Crit Care Med (2014) 1.00

Cystic fibrosis transmembrane conductance regulator (CFTR) allelic variants relate to shifts in faecal microbiota of cystic fibrosis patients. PLoS One (2013) 0.99

Respiratory microbiota: addressing clinical questions, informing clinical practice. Thorax (2014) 0.98

The microbiome and the lung. Ann Am Thorac Soc (2014) 0.97

Directly sampling the lung of a young child with cystic fibrosis reveals diverse microbiota. Ann Am Thorac Soc (2014) 0.97

Lung phenotype of juvenile and adult cystic fibrosis transmembrane conductance regulator-knockout ferrets. Am J Respir Cell Mol Biol (2014) 0.97

Interpreting infective microbiota: the importance of an ecological perspective. Trends Microbiol (2013) 0.96

Bacterial microbiome of lungs in COPD. Int J Chron Obstruct Pulmon Dis (2014) 0.94

Microbial, host and xenobiotic diversity in the cystic fibrosis sputum metabolome. ISME J (2015) 0.93

The microbiome in pediatric cystic fibrosis patients: the role of shared environment suggests a window of intervention. Microbiome (2014) 0.91

Severe viral respiratory infections: are bugs bugging? Mucosal Immunol (2013) 0.90

Abundant DNase I-sensitive bacterial DNA in healthy porcine lungs and its implications for the lung microbiome. Appl Environ Microbiol (2013) 0.90

16S community profiling identifies proton pump inhibitor related differences in gastric, lung, and oropharyngeal microflora. J Pediatr (2015) 0.90

Cheating by type 3 secretion system-negative Pseudomonas aeruginosa during pulmonary infection. Proc Natl Acad Sci U S A (2014) 0.90

The microbiota in bronchoalveolar lavage from young children with chronic lung disease includes taxa present in both the oropharynx and nasopharynx. Microbiome (2016) 0.89

Bdellovibrio bacteriovorus directly attacks Pseudomonas aeruginosa and Staphylococcus aureus Cystic fibrosis isolates. Front Microbiol (2014) 0.89

The Composition of Microbiome in Larynx and the Throat Biodiversity between Laryngeal Squamous Cell Carcinoma Patients and Control Population. PLoS One (2013) 0.89

ABCs of the lung microbiome. Ann Am Thorac Soc (2014) 0.89

Vaccines for Pseudomonas aeruginosa: a long and winding road. Expert Rev Vaccines (2014) 0.89

Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections. Clin Microbiol Infect (2015) 0.89

Homeostasis and its disruption in the lung microbiome. Am J Physiol Lung Cell Mol Physiol (2015) 0.87

Toward implementation of quorum sensing autoinducers as biomarkers for infectious disease states. Anal Chem (2013) 0.87

MetaGeniE: characterizing human clinical samples using deep metagenomic sequencing. PLoS One (2014) 0.86

Impact of the CFTR-potentiator ivacaftor on airway microbiota in cystic fibrosis patients carrying a G551D mutation. PLoS One (2015) 0.86

The microbial community of the cystic fibrosis airway is disrupted in early life. PLoS One (2014) 0.86

Back to the future: evolving bacteriophages to increase their effectiveness against the pathogen Pseudomonas aeruginosa PAO1. Evol Appl (2013) 0.86

Analysis of Lung Microbiota in Bronchoalveolar Lavage, Protected Brush and Sputum Samples from Subjects with Mild-To-Moderate Cystic Fibrosis Lung Disease. PLoS One (2016) 0.84

Analysis of changes in diversity and abundance of the microbial community in a cystic fibrosis patient over a multiyear period. J Clin Microbiol (2014) 0.84

A Winogradsky-based culture system shows an association between microbial fermentation and cystic fibrosis exacerbation. ISME J (2015) 0.84

Assessment of IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis by an enzyme-linked immunosorbent assay (ELISA). Diagn Pathol (2014) 0.81

Cystic fibrosis mouse model-dependent intestinal structure and gut microbiome. Mamm Genome (2015) 0.80

Microbiota in the Throat and Risk Factors for Laryngeal Carcinoma. Appl Environ Microbiol (2014) 0.80

The microbiome of the human lower airways: a next generation sequencing perspective. World Allergy Organ J (2015) 0.79

The microbiome at the pulmonary alveolar niche and its role in Mycobacterium tuberculosis infection. Tuberculosis (Edinb) (2015) 0.79

Longitudinal sampling of the lung microbiota in individuals with cystic fibrosis. PLoS One (2017) 0.78

Association of sputum microbiota profiles with severity of community-acquired pneumonia in children. BMC Infect Dis (2016) 0.78

The pediatric microbiome and the lung. Curr Opin Pediatr (2015) 0.77

The lung microbiome after lung transplantation. Expert Rev Respir Med (2014) 0.77

Lungs, microbes and the developing neonate. Neonatology (2015) 0.77

Throat Swabs and Sputum Culture as Predictors of P. aeruginosa or S. aureus Lung Colonization in Adult Cystic Fibrosis Patients. PLoS One (2016) 0.77

Lung Microbiota Changes Associated with Chronic Pseudomonas aeruginosa Lung Infection and the Impact of Intravenous Colistimethate Sodium. PLoS One (2015) 0.76

Harnessing the Early-Life Microbiota to Protect Children with Cystic Fibrosis. J Pediatr (2015) 0.75

Lung Microbiota and Its Impact on the Mucosal Immune Phenotype. Microbiol Spectr (2017) 0.75

The CF gastrointestinal microbiome: Structure and clinical impact. Pediatr Pulmonol (2016) 0.75

Mycoplasma pneumoniae and Streptococcus pneumoniae caused different microbial structure and correlation network in lung microbiota. J Thorac Dis (2016) 0.75

Effects of Propidium Monoazide (PMA) Treatment on Mycobiome and Bacteriome Analysis of Cystic Fibrosis Airways during Exacerbation. PLoS One (2016) 0.75

Microbiome effects on immunity, health and disease in the lung. Clin Transl Immunology (2017) 0.75

Acquisition and adaptation of the airway microbiota in the early life of cystic fibrosis patients. Mol Cell Pediatr (2017) 0.75

Ability of device to collect bacteria from cough aerosols generated by adults with cystic fibrosis. F1000Res (2016) 0.75

The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients. Exp Mol Med (2017) 0.75

New Technologies for Studying Biofilms. Microbiol Spectr (2015) 0.75

Republished: Respiratory microbiota: addressing clinical questions, informing clinical practice. Postgrad Med J (2015) 0.75

Understanding persistent bacterial lung infections: clinical implications informed by the biology of the microbiota and biofilms. Clin Pulm Med (2016) 0.75

Dynamic changes of the respiratory microbiota and its relationship to fecal and blood microbiota in healthy young cats. PLoS One (2017) 0.75

Respiratory Microbiome of New-Born Infants. Front Pediatr (2016) 0.75

Microbiome Changes during Tuberculosis and Antituberculous Therapy. Clin Microbiol Rev (2016) 0.75

Microbial Communities of Conducting and Respiratory Zones of Lung-Transplanted Patients. Front Microbiol (2016) 0.75

Three distinct pneumotypes characterize the microbiome of the lung in BALB/cJ mice. PLoS One (2017) 0.75

Individual Patterns of Complexity in Cystic Fibrosis Lung Microbiota, Including Predator Bacteria, over a 1-Year Period. MBio (2017) 0.75

Composition and abundance of microbiota in the pharynx in patients with laryngeal carcinoma and vocal cord polyps. J Microbiol (2017) 0.75

Influence of lung CT changes in chronic obstructive pulmonary disease (COPD) on the human lung microbiome. PLoS One (2017) 0.75

How can the cystic fibrosis respiratory microbiome influence our clinical decision-making? Curr Opin Pulm Med (2017) 0.75

Articles cited by this

QIIME allows analysis of high-throughput community sequencing data. Nat Methods (2010) 85.34

Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol (2007) 60.97

Limitations of next-generation genome sequence assembly. Nat Methods (2010) 9.04

Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients. J Clin Invest (2002) 6.35

Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). BMC Microbiol (2008) 6.28

Defining the healthy "core microbiome" of oral microbial communities. BMC Microbiol (2009) 4.79

Specific ribosomal DNA sequences from diverse environmental settings correlate with experimental contaminants. Appl Environ Microbiol (1998) 4.08

Molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis. Proc Natl Acad Sci U S A (2007) 4.06

Bronchoalveolar lavage or oropharyngeal cultures to identify lower respiratory pathogens in infants with cystic fibrosis. Pediatr Pulmonol (1996) 3.49

Decade-long bacterial community dynamics in cystic fibrosis airways. Proc Natl Acad Sci U S A (2012) 3.43

Airway microbiota and pathogen abundance in age-stratified cystic fibrosis patients. PLoS One (2010) 3.38

Polymerase chain reaction detection of nonviable bacterial pathogens. Appl Environ Microbiol (1993) 3.04

Exacerbations in cystic fibrosis. 1: Epidemiology and pathogenesis. Thorax (2007) 2.60

Bacterial diversity in cases of lung infection in cystic fibrosis patients: 16S ribosomal DNA (rDNA) length heterogeneity PCR and 16S rDNA terminal restriction fragment length polymorphism profiling. J Clin Microbiol (2003) 2.56

Relationship between cystic fibrosis respiratory tract bacterial communities and age, genotype, antibiotics and Pseudomonas aeruginosa. Environ Microbiol (2010) 2.55

Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities. ISME J (2010) 2.55

Phylogenetic and metabolic diversity of bacteria associated with cystic fibrosis. ISME J (2010) 2.33

Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes. Environ Microbiol (2011) 2.28

Clinical significance of microbial infection and adaptation in cystic fibrosis. Clin Microbiol Rev (2011) 2.23

Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients. PLoS One (2008) 2.23

Target region selection is a critical determinant of community fingerprints generated by 16S pyrosequencing. PLoS One (2011) 2.04

Black Box Chimera Check (B2C2): a Windows-Based Software for Batch Depletion of Chimeras from Bacterial 16S rRNA Gene Datasets. Open Microbiol J (2010) 1.94

Spatial distribution of microbial communities in the cystic fibrosis lung. ISME J (2011) 1.83

Diversity of the human skin microbiome early in life. J Invest Dermatol (2011) 1.77

Massive parallel 16S rRNA gene pyrosequencing reveals highly diverse fecal bacterial and fungal communities in healthy dogs and cats. FEMS Microbiol Ecol (2011) 1.72

Bacterial diversity in Solenopsis invicta and Solenopsis geminata ant colonies characterized by 16S amplicon 454 pyrosequencing. Microb Ecol (2011) 1.69

Reliability of quantitative real-time PCR for bacterial detection in cystic fibrosis airway specimens. PLoS One (2010) 1.37

Analysis of the bacterial communities present in lungs of patients with cystic fibrosis from American and British centers. J Clin Microbiol (2010) 1.32

Spatial Distribution and Coexistence of Klebsiella pneumoniae and Pseudomonas aeruginosa in Biofilms Microb Ecol (1997) 1.30

Changes in cystic fibrosis sputum microbiology in the United States between 1995 and 2008. Pediatr Pulmonol (2010) 1.29

Maternal methyl-donor supplementation induces prolonged murine offspring colitis susceptibility in association with mucosal epigenetic and microbiomic changes. Hum Mol Genet (2011) 1.26

Comparison of six different criteria for judging the acceptability of sputum specimens. J Clin Microbiol (1982) 1.19

The relevance of the polymicrobial nature of airway infection in the acute and chronic management of patients with cystic fibrosis. Curr Opin Investig Drugs (2009) 1.19

The airway microbiome in cystic fibrosis and implications for treatment. Curr Opin Pediatr (2011) 1.17

Regional variability of lung inflammation in cystic fibrosis. Am J Respir Crit Care Med (1997) 1.12

Detection of live and antibiotic-killed bacteria by quantitative real-time PCR of specific fragments of rRNA. Antimicrob Agents Chemother (2006) 0.96

True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis. J Clin Microbiol (2011) 0.94

Development of real-time PCR assays for detection of the Streptococcus milleri group from cystic fibrosis clinical specimens by targeting the cpn60 and 16S rRNA genes. J Clin Microbiol (2010) 0.94

Utility of gram stain in evaluation of sputa from patients with cystic fibrosis. J Clin Microbiol (1994) 0.93

Articles by these authors

Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol (2003) 7.29

Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). BMC Microbiol (2008) 6.28

Effect of VX-770 in persons with cystic fibrosis and the G551D-CFTR mutation. N Engl J Med (2010) 6.23

Self-generated diversity produces "insurance effects" in biofilm communities. Proc Natl Acad Sci U S A (2004) 4.54

Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing. BMC Microbiol (2008) 4.30

Active starvation responses mediate antibiotic tolerance in biofilms and nutrient-limited bacteria. Science (2011) 3.66

Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP). PLoS One (2008) 2.87

Results of a phase IIa study of VX-809, an investigational CFTR corrector compound, in subjects with cystic fibrosis homozygous for the F508del-CFTR mutation. Thorax (2011) 2.84

Exposure to a social stressor alters the structure of the intestinal microbiota: implications for stressor-induced immunomodulation. Brain Behav Immun (2010) 2.69

Cystic fibrosis sputum supports growth and cues key aspects of Pseudomonas aeruginosa physiology. J Bacteriol (2005) 2.59

Pyrosequencing study of fecal microflora of autistic and control children. Anaerobe (2010) 2.51

Developing cystic fibrosis lung transplant referral criteria using predictors of 2-year mortality. Am J Respir Crit Care Med (2002) 2.50

Rhamnolipids mediate detachment of Pseudomonas aeruginosa from biofilms. Mol Microbiol (2005) 2.40

Effect of genotype on phenotype and mortality in cystic fibrosis: a retrospective cohort study. Lancet (2003) 2.36

Inflammatory and microbiologic markers in induced sputum after intravenous antibiotics in cystic fibrosis. Am J Respir Crit Care Med (2003) 2.26

The transition metal gallium disrupts Pseudomonas aeruginosa iron metabolism and has antimicrobial and antibiofilm activity. J Clin Invest (2007) 2.26

ExsD is a negative regulator of the Pseudomonas aeruginosa type III secretion regulon. Mol Microbiol (2002) 2.18

Exploring the diversity of Gardnerella vaginalis in the genitourinary tract microbiota of monogamous couples through subtle nucleotide variation. PLoS One (2011) 2.06

Stressor exposure disrupts commensal microbial populations in the intestines and leads to increased colonization by Citrobacter rodentium. Infect Immun (2010) 2.06

Target region selection is a critical determinant of community fingerprints generated by 16S pyrosequencing. PLoS One (2011) 2.04

Association between pulmonary function and sputum biomarkers in cystic fibrosis. Am J Respir Crit Care Med (2007) 2.03

Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing. BMC Microbiol (2011) 2.02

Endogenous oxidative stress produces diversity and adaptability in biofilm communities. Proc Natl Acad Sci U S A (2008) 2.00

Effectiveness of screening examinations to detect unrecognized exercise-induced bronchoconstriction. J Pediatr (2002) 1.96

Phylogenetic and gene-centric metagenomics of the canine intestinal microbiome reveals similarities with humans and mice. ISME J (2010) 1.95

Black Box Chimera Check (B2C2): a Windows-Based Software for Batch Depletion of Chimeras from Bacterial 16S rRNA Gene Datasets. Open Microbiol J (2010) 1.94

Evolving stealth: genetic adaptation of Pseudomonas aeruginosa during cystic fibrosis infections. Proc Natl Acad Sci U S A (2006) 1.93

Repeated adeno-associated virus serotype 2 aerosol-mediated cystic fibrosis transmembrane regulator gene transfer to the lungs of patients with cystic fibrosis: a multicenter, double-blind, placebo-controlled trial. Chest (2004) 1.91

Generalized antifungal activity and 454-screening of Pseudonocardia and Amycolatopsis bacteria in nests of fungus-growing ants. Proc Natl Acad Sci U S A (2009) 1.80

Prevalence of neutralizing antibodies against adeno-associated virus (AAV) types 2, 5, and 6 in cystic fibrosis and normal populations: Implications for gene therapy using AAV vectors. Hum Gene Ther (2006) 1.78

Diversity of the human skin microbiome early in life. J Invest Dermatol (2011) 1.77

Massive parallel 16S rRNA gene pyrosequencing reveals highly diverse fecal bacterial and fungal communities in healthy dogs and cats. FEMS Microbiol Ecol (2011) 1.72

Bacterial diversity in Solenopsis invicta and Solenopsis geminata ant colonies characterized by 16S amplicon 454 pyrosequencing. Microb Ecol (2011) 1.69

Use of nasal potential difference and sweat chloride as outcome measures in multicenter clinical trials in subjects with cystic fibrosis. Pediatr Pulmonol (2002) 1.67

Disparities in access to lung transplantation for patients with cystic fibrosis by socioeconomic status. Am J Respir Crit Care Med (2012) 1.64

Culture enriched molecular profiling of the cystic fibrosis airway microbiome. PLoS One (2011) 1.61

Risk of post-lung transplant renal dysfunction in adults with cystic fibrosis. Chest (2012) 1.60

Respiratory outbreak of Mycobacterium abscessus subspecies massiliense in a lung transplant and cystic fibrosis center. Am J Respir Crit Care Med (2012) 1.59

β-cell loss and β-cell apoptosis in human type 2 diabetes are related to islet amyloid deposition. Am J Pathol (2011) 1.59

Spectral index for assessment of differential protein expression in shotgun proteomics. J Proteome Res (2008) 1.55

Effects of bronchoconstriction, minute ventilation, and deep inspiration on the composition of exhaled breath condensate. Chest (2010) 1.54

Bacterial neuraminidase facilitates mucosal infection by participating in biofilm production. J Clin Invest (2006) 1.54

Risk factors for chronic kidney disease in adults with cystic fibrosis. Am J Respir Crit Care Med (2011) 1.53

The extracellular matrix protects Pseudomonas aeruginosa biofilms by limiting the penetration of tobramycin. Environ Microbiol (2013) 1.47

Detecting Stenotrophomonas maltophilia does not reduce survival of patients with cystic fibrosis. Am J Respir Crit Care Med (2002) 1.46

Evaluation of the bacterial diversity among and within individual venous leg ulcers using bacterial tag-encoded FLX and titanium amplicon pyrosequencing and metagenomic approaches. BMC Microbiol (2009) 1.45

Delayed wound healing in diabetic (db/db) mice with Pseudomonas aeruginosa biofilm challenge: a model for the study of chronic wounds. Wound Repair Regen (2010) 1.44

Small RNA profiling reveals antisense transcription throughout the KSHV genome and novel small RNAs. RNA (2010) 1.44

PLUNC is a novel airway surfactant protein with anti-biofilm activity. PLoS One (2010) 1.44

The fecal microbiome in dogs with acute diarrhea and idiopathic inflammatory bowel disease. PLoS One (2012) 1.43

Memory and learning behavior in mice is temporally associated with diet-induced alterations in gut bacteria. Physiol Behav (2008) 1.42

A comparison of bacterial composition in diabetic ulcers and contralateral intact skin. Open Microbiol J (2010) 1.39

Soil bacteria confer plant salt tolerance by tissue-specific regulation of the sodium transporter HKT1. Mol Plant Microbe Interact (2008) 1.39

Targeting a bacterial stress response to enhance antibiotic action. Proc Natl Acad Sci U S A (2009) 1.39

Single-copy genes define a conserved order between rice and wheat for understanding differences caused by duplication, deletion, and transposition of genes. Funct Integr Genomics (2006) 1.38

Autopsy utilization in medicolegal defense of anesthesiologists. Anesthesiology (2011) 1.38

The effect of the macrolide antibiotic tylosin on microbial diversity in the canine small intestine as demonstrated by massive parallel 16S rRNA gene sequencing. BMC Microbiol (2009) 1.36

Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. PLoS One (2011) 1.36

An in vivo polymicrobial biofilm wound infection model to study interspecies interactions. PLoS One (2011) 1.34

Frequency-dependent response of the vascular endothelium to pulsatile shear stress. Am J Physiol Heart Circ Physiol (2007) 1.33

Comparative metagenomic analysis of soil microbial communities across three hexachlorocyclohexane contamination levels. PLoS One (2012) 1.33

CFTR genotype as a predictor of prognosis in cystic fibrosis. Chest (2006) 1.31

16S rRNA gene pyrosequencing reveals bacterial dysbiosis in the duodenum of dogs with idiopathic inflammatory bowel disease. PLoS One (2012) 1.30

Pseudomonas aeruginosa acquires biofilm-like properties within airway epithelial cells. Infect Immun (2005) 1.30

454 pyrosequencing reveals a shift in fecal microbiota of healthy adult men consuming polydextrose or soluble corn fiber. J Nutr (2012) 1.30

Radiographically occult, diffuse intrasinusoidal hepatic metastases from primary breast carcinomas: a clinicopathologic study of 3 autopsy cases. Arch Pathol Lab Med (2004) 1.29

Inflammation drives dysbiosis and bacterial invasion in murine models of ileal Crohn's disease. PLoS One (2012) 1.28

Inflammatory basis of exercise-induced bronchoconstriction. Am J Respir Crit Care Med (2005) 1.28

Evaluation of the bacterial diversity of pressure ulcers using bTEFAP pyrosequencing. BMC Med Genomics (2010) 1.28

The rehydration transcriptome of the desiccation-tolerant bryophyte Tortula ruralis: transcript classification and analysis. BMC Genomics (2004) 1.27

Maternal methyl-donor supplementation induces prolonged murine offspring colitis susceptibility in association with mucosal epigenetic and microbiomic changes. Hum Mol Genet (2011) 1.26

Tag-encoded FLX amplicon pyrosequencing for the elucidation of microbial and functional gene diversity in any environment. Methods Mol Biol (2011) 1.26

Detection of protozoan parasites and microsporidia in irrigation waters used for crop production. J Food Prot (2002) 1.22

Effect of the proton pump inhibitor omeprazole on the gastrointestinal bacterial microbiota of healthy dogs. FEMS Microbiol Ecol (2012) 1.21

High-level relatedness among Mycobacterium abscessus subsp. massiliense strains from widely separated outbreaks. Emerg Infect Dis (2014) 1.21

Conditions associated with the cystic fibrosis defect promote chronic Pseudomonas aeruginosa infection. Am J Respir Crit Care Med (2014) 1.19

Mucin-Pseudomonas aeruginosa interactions promote biofilm formation and antibiotic resistance. Mol Microbiol (2006) 1.19

Characterization of a newly developed chicken 44K Agilent microarray. BMC Genomics (2008) 1.17

A soil bacterium regulates plant acquisition of iron via deficiency-inducible mechanisms. Plant J (2009) 1.17

Gene-centric metagenomics analysis of feline intestinal microbiome using 454 junior pyrosequencing. J Microbiol Methods (2012) 1.16

Distinct soil bacterial communities revealed under a diversely managed agroecosystem. PLoS One (2012) 1.14

Molecular characterization of the polymicrobial flora in chronic rhinosinusitis. J Otolaryngol Head Neck Surg (2010) 1.14

Imaging of the diaphragm: anatomy and function. Radiographics (2012) 1.13

Airway immunopathology of asthma with exercise-induced bronchoconstriction. J Allergy Clin Immunol (2005) 1.12

In vitro multispecies Lubbock chronic wound biofilm model. Wound Repair Regen (2009) 1.12

Comparison of culture and molecular identification of bacteria in chronic wounds. Int J Mol Sci (2012) 1.10

Microbial dysbiosis in pediatric patients with Crohn's disease. J Clin Microbiol (2012) 1.10

Microbiome analysis among bats describes influences of host phylogeny, life history, physiology and geography. Mol Ecol (2012) 1.09

The Parkinson's disease-associated H50Q mutation accelerates α-Synuclein aggregation in vitro. Biochemistry (2013) 1.08

No detectable improvements in cystic fibrosis transmembrane conductance regulator by nasal aminoglycosides in patients with cystic fibrosis with stop mutations. Am J Respir Cell Mol Biol (2007) 1.08