Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.

PubWeight™: 1.00‹?› | Rank: Top 15%

🔗 View Article (PMC 3553786)

Published in Appl Environ Microbiol on November 02, 2012

Authors

F Heath Damron1, Elizabeth S McKenney, Herbert P Schweizer, Joanna B Goldberg

Author Affiliations

1: Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA.

Articles citing this

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis. Appl Environ Microbiol (2013) 1.00

Non-equivalent roles of two periplasmic subunits in the function and assembly of triclosan pump TriABC from Pseudomonas aeruginosa. Mol Microbiol (2015) 0.78

A Modular, Tn7-Based System for Making Bioluminescent or Fluorescent Salmonella and Escherichia coli Strains. Appl Environ Microbiol (2016) 0.78

The Pseudomonas aeruginosa PAO1 Two-Component Regulator CarSR Regulates Calcium Homeostasis and Calcium-Induced Virulence Factor Production through Its Regulatory Targets CarO and CarP. J Bacteriol (2016) 0.77

The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System Allows Tightly Controlled Gene Expression over a Wide Range in Pseudomonas aeruginosa. Appl Environ Microbiol (2016) 0.75

An Oxygen-Sensing Two-Component System in the Burkholderia cepacia Complex Regulates Biofilm, Intracellular Invasion, and Pathogenicity. PLoS Pathog (2017) 0.75

Pseudomonas aeruginosa AlgU Contributes to Posttranscriptional Activity by Increasing rsmA Expression in a mucA22 Strain. J Bacteriol (2016) 0.75

Mini-Tn7 Insertion in an Artificial attTn7 Site Enables Depletion of the Essential Master Regulator CtrA in the Phytopathogen Agrobacterium tumefaciens. Appl Environ Microbiol (2016) 0.75

An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria. J Microbiol Methods (2015) 0.75

The RhlR quorum-sensing receptor controls Pseudomonas aeruginosa pathogenesis and biofilm development independently of its canonical homoserine lactone autoinducer. PLoS Pathog (2017) 0.75

Articles cited by this

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol (1995) 46.37

Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A (1979) 44.75

Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A (2003) 10.63

An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A (2006) 7.62

A Tn7-based broad-range bacterial cloning and expression system. Nat Methods (2005) 4.66

mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat Protoc (2006) 3.19

The broad host range pathogen Pseudomonas aeruginosa strain PA14 carries two pathogenicity islands harboring plant and animal virulence genes. Proc Natl Acad Sci U S A (2004) 3.01

Integration-proficient Pseudomonas aeruginosa vectors for isolation of single-copy chromosomal lacZ and lux gene fusions. Biotechniques (2000) 2.73

Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Appl Environ Microbiol (2007) 2.40

PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria. Appl Environ Microbiol (2008) 2.16

An expression vector containing a rhamnose-inducible promoter provides tightly regulated gene expression in Burkholderia cenocepacia. Plasmid (2005) 1.72

mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344. Nat Protoc (2006) 1.58

The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis. J Bacteriol (2009) 1.41

Single-copy chromosomal integration systems for Francisella tularensis. Microbiology (2009) 1.34

Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei. Proc Natl Acad Sci U S A (2011) 1.29

mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320. Nat Protoc (2006) 1.20

Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii. J Microbiol Methods (2010) 1.02

Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp. FEMS Microbiol Lett (2009) 0.92

Articles by these authors

A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J Microbiol Methods (2005) 6.02

The multifarious, multireplicon Burkholderia cepacia complex. Nat Rev Microbiol (2005) 5.38

Parallel bacterial evolution within multiple patients identifies candidate pathogenicity genes. Nat Genet (2011) 4.81

A Tn7-based broad-range bacterial cloning and expression system. Nat Methods (2005) 4.66

mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat Protoc (2006) 3.19

Toll-like receptor (TLR) 2 and TLR5, but not TLR4, are required for Helicobacter pylori-induced NF-kappa B activation and chemokine expression by epithelial cells. J Biol Chem (2003) 2.72

An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants. BMC Microbiol (2005) 2.53

Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Appl Environ Microbiol (2007) 2.40

Management of accidental laboratory exposure to Burkholderia pseudomallei and B. mallei. Emerg Infect Dis (2008) 2.24

PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria. Appl Environ Microbiol (2008) 2.16

Two aerobic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosa. Mol Microbiol (2006) 2.05

The MexJK efflux pump of Pseudomonas aeruginosa requires OprM for antibiotic efflux but not for efflux of triclosan. J Bacteriol (2002) 1.76

Versatile dual-technology system for markerless allele replacement in Burkholderia pseudomallei. Appl Environ Microbiol (2009) 1.74

Bacterial resistance to antibiotics: active efflux and reduced uptake. Adv Drug Deliv Rev (2005) 1.71

mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344. Nat Protoc (2006) 1.58

Human airway epithelial cells sense Pseudomonas aeruginosa infection via recognition of flagellin by Toll-like receptor 5. Infect Immun (2005) 1.51

The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux system. Antimicrob Agents Chemother (2010) 1.50

Pseudomonas aeruginosa-plant root interactions. Pathogenicity, biofilm formation, and root exudation. Plant Physiol (2003) 1.50

Workshop on treatment of and postexposure prophylaxis for Burkholderia pseudomallei and B. mallei Infection, 2010. Emerg Infect Dis (2012) 1.48

Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1. Microbiology (2003) 1.46

Present and future therapeutic strategies for melioidosis and glanders. Expert Rev Anti Infect Ther (2010) 1.45

IL-17 is a critical component of vaccine-induced protection against lung infection by lipopolysaccharide-heterologous strains of Pseudomonas aeruginosa. J Immunol (2008) 1.42

Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI. Mol Microbiol (2004) 1.40

Metabolic network analysis of Pseudomonas aeruginosa during chronic cystic fibrosis lung infection. J Bacteriol (2010) 1.37

Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins. J Bacteriol (2007) 1.36

Single-copy chromosomal integration systems for Francisella tularensis. Microbiology (2009) 1.34

Molecular basis of rare aminoglycoside susceptibility and pathogenesis of Burkholderia pseudomallei clinical isolates from Thailand. PLoS Negl Trop Dis (2009) 1.34

Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b. Antimicrob Agents Chemother (2006) 1.33

Proteolytic regulation of alginate overproduction in Pseudomonas aeruginosa. Mol Microbiol (2012) 1.31

Molecular basis of azithromycin-resistant Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother (2005) 1.30

Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei. Proc Natl Acad Sci U S A (2011) 1.29

Substrate-dependent utilization of OprM or OpmH by the Pseudomonas aeruginosa MexJK efflux pump. Antimicrob Agents Chemother (2005) 1.29

A Burkholderia pseudomallei deltapurM mutant is avirulent in immunocompetent and immunodeficient animals: candidate strain for exclusion from select-agent lists. Infect Immun (2010) 1.26

Molecular Investigations of PenA-mediated β-lactam Resistance in Burkholderia pseudomallei. Front Microbiol (2011) 1.25

Pseudomonas aeruginosa galU is required for a complete lipopolysaccharide core and repairs a secondary mutation in a PA103 (serogroup O11) wbpM mutant. FEMS Microbiol Lett (2002) 1.23

Contribution of Burkholderia cenocepacia flagella to infectivity and inflammation. Infect Immun (2004) 1.22

Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis. J Bacteriol (2005) 1.20

mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320. Nat Protoc (2006) 1.20

The galU Gene of Pseudomonas aeruginosa is required for corneal infection and efficient systemic spread following pneumonia but not for infection confined to the lung. Infect Immun (2004) 1.18

High-level triclosan resistance in Pseudomonas aeruginosa is solely a result of efflux. Am J Infect Control (2003) 1.18

Intranasal immunization with heterologously expressed polysaccharide protects against multiple Pseudomonas aeruginosa infections. Proc Natl Acad Sci U S A (2007) 1.17

Beta-ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths. Microbiology (2002) 1.16

Lipopolysaccharide of Burkholderia cepacia complex. J Endotoxin Res (2003) 1.15

Helicobacter pylori infection, oncogenic pathways and epigenetic mechanisms in gastric carcinogenesis. Future Oncol (2010) 1.14

In vivo Bioluminescence Imaging of Burkholderia mallei Respiratory Infection and Treatment in the Mouse Model. Front Microbiol (2011) 1.14

Recurrent Burkholderia infection in patients with chronic granulomatous disease: 11-year experience at a large referral center. Clin Infect Dis (2009) 1.14

Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival. J Proteomics (2011) 1.13

Protection against fatal Pseudomonas aeruginosa pneumonia in mice after nasal immunization with a live, attenuated aroA deletion mutant. Infect Immun (2003) 1.13

Nitrogen redox balance in the cystic fibrosis airway: effects of antipseudomonal therapy. Am J Respir Crit Care Med (2002) 1.09

Strategies toward vaccines against Burkholderia mallei and Burkholderia pseudomallei. Expert Rev Vaccines (2008) 1.09

Benchtop and microcentrifuge preparation of Pseudomonas aeruginosa competent cells. Biotechniques (2002) 1.09

Immunotherapy markedly increases the effectiveness of antimicrobial therapy for treatment of Burkholderia pseudomallei infection. Antimicrob Agents Chemother (2010) 1.08

Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia. Trans R Soc Trop Med Hyg (2008) 1.08

Novel phosphorylcholine-containing protein of Pseudomonas aeruginosa chronic infection isolates interacts with airway epithelial cells. J Infect Dis (2008) 1.08

In vivo Himar1 transposon mutagenesis of Burkholderia pseudomallei. Appl Environ Microbiol (2008) 1.08

Construction and characterization of a live, attenuated aroA deletion mutant of Pseudomonas aeruginosa as a candidate intranasal vaccine. Infect Immun (2002) 1.08

Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration. EMBO J (2007) 1.07

Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN. J Bacteriol (2011) 1.06

Oral vaccination of BALB/c mice with Salmonella enterica serovar Typhimurium expressing Pseudomonas aeruginosa O antigen promotes increased survival in an acute fatal pneumonia model. Infect Immun (2004) 1.03

Helicobacter pylori induces interleukin-8 secretion by Toll-like receptor 2- and Toll-like receptor 5-dependent and -independent pathways. Infect Immun (2005) 1.03

Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii. J Microbiol Methods (2010) 1.02

The structure of the lipopolysaccharide from a galU mutant of Pseudomonas aeruginosa serogroup-O11. Carbohydr Res (2005) 1.02

Toll-like receptor 2-mediated gene expression in epithelial cells during Helicobacter pylori infection. Helicobacter (2005) 1.01

In vitro activity of BAL30072 against Burkholderia pseudomallei. Int J Antimicrob Agents (2011) 1.01

Pseudomonas aeruginosa 1244 pilin glycosylation: glycan substrate recognition. J Bacteriol (2006) 1.01

Overexpression of Mycobacterium tuberculosis manB, a phosphomannomutase that increases phosphatidylinositol mannoside biosynthesis in Mycobacterium smegmatis and mycobacterial association with human macrophages. Mol Microbiol (2005) 1.00

A high-throughput, homogeneous, bioluminescent assay for Pseudomonas aeruginosa gyrase inhibitors and other DNA-damaging agents. J Biomol Screen (2007) 1.00

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis. Appl Environ Microbiol (2013) 1.00

Cable pili and the 22-kilodalton adhesin are required for Burkholderia cenocepacia binding to and transmigration across the squamous epithelium. Infect Immun (2005) 0.98

Adaptability and persistence of the emerging pathogen Bordetella petrii. PLoS One (2013) 0.97

Mucosal vaccination with a multivalent, live-attenuated vaccine induces multifactorial immunity against Pseudomonas aeruginosa acute lung infection. Infect Immun (2010) 0.97

Does microbicide use in consumer products promote antimicrobial resistance? A critical review and recommendations for a cohesive approach to risk assessment. Microb Drug Resist (2013) 0.96

Targeting pan-resistant bacteria with antibodies to a broadly conserved surface polysaccharide expressed during infection. J Infect Dis (2012) 0.96

Structural basis for the interaction of lipopolysaccharide with outer membrane protein H (OprH) from Pseudomonas aeruginosa. J Biol Chem (2011) 0.96

Structural determinants of inhibitor selectivity in prokaryotic IMP dehydrogenases. Chem Biol (2010) 0.96

Vaccination against Pseudomonas aeruginosa pneumonia in immunocompromised mice. Clin Vaccine Immunol (2007) 0.94

Helicobacter pylori and mitogen-activated protein kinases regulate the cell cycle, proliferation and apoptosis in gastric epithelial cells. J Gastroenterol Hepatol (2008) 0.93

Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders. Front Cell Infect Microbiol (2012) 0.93

Molecular characterization of MexL, the transcriptional repressor of the mexJK multidrug efflux operon in Pseudomonas aeruginosa. Antimicrob Agents Chemother (2005) 0.92

Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp. FEMS Microbiol Lett (2009) 0.92

WbjA adds glucose to complete the O-antigen trisaccharide repeating unit of the lipopolysaccharide of Pseudomonas aeruginosa serogroup O11. J Bacteriol (2002) 0.92

Comparative metabolic systems analysis of pathogenic Burkholderia. J Bacteriol (2013) 0.91

Vanadate and triclosan synergistically induce alginate production by Pseudomonas aeruginosa strain PAO1. Mol Microbiol (2011) 0.91

Helicobacter pylori-induced histone modification, associated gene expression in gastric epithelial cells, and its implication in pathogenesis. PLoS One (2010) 0.91

An improved method for oriT-directed cloning and functionalization of large bacterial genomic regions. Appl Environ Microbiol (2013) 0.90

A novel oxidized low-density lipoprotein-binding protein from Pseudomonas aeruginosa. Microbiology (2008) 0.90

Genetic characterization of pcpS, encoding the multifunctional phosphopantetheinyl transferase of Pseudomonas aeruginosa. Microbiology (2004) 0.90

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Front Microbiol (2011) 0.90

Virulence and cellular interactions of Burkholderia multivorans in chronic granulomatous disease. Infect Immun (2009) 0.90

Purification and visualization of lipopolysaccharide from Gram-negative bacteria by hot aqueous-phenol extraction. J Vis Exp (2012) 0.90

Pseudomonas aeruginosa alginate promotes Burkholderia cenocepacia persistence in cystic fibrosis transmembrane conductance regulator knockout mice. Infect Immun (2010) 0.89

Outer membrane biogenesis in Escherichia coli, Neisseria meningitidis, and Helicobacter pylori: paradigm deviations in H. pylori. Front Cell Infect Microbiol (2012) 0.89

Draft Genome Sequences of Burkholderia cenocepacia ET12 Lineage Strains K56-2 and BC7. Genome Announc (2013) 0.89

Regulation of lipopolysaccharide O antigen expression in Pseudomonas aeruginosa. Future Microbiol (2008) 0.89

Vaccines for Pseudomonas aeruginosa: a long and winding road. Expert Rev Vaccines (2014) 0.89

The Burkholderia pseudomallei Δasd mutant exhibits attenuated intracellular infectivity and imparts protection against acute inhalation melioidosis in mice. Infect Immun (2011) 0.89

Helicobacter pylori and mitogen-activated protein kinases mediate activator protein-1 (AP-1) subcomponent protein expression and DNA-binding activity in gastric epithelial cells. FEMS Immunol Med Microbiol (2008) 0.88

Burkholderia pseudomallei known siderophores and hemin uptake are dispensable for lethal murine melioidosis. PLoS Negl Trop Dis (2012) 0.88