Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b.

PubWeight™: 1.33‹?› | Rank: Top 10%

🔗 View Article (PMC 1610100)

Published in Antimicrob Agents Chemother on October 01, 2006

Authors

Ayush Kumar1, Kim-Lee Chua, Herbert P Schweizer

Author Affiliations

1: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA.

Articles citing this

Efflux-mediated drug resistance in bacteria: an update. Drugs (2009) 3.18

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria. Clin Microbiol Rev (2015) 1.81

Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance. Clin Microbiol Rev (2012) 1.74

Versatile dual-technology system for markerless allele replacement in Burkholderia pseudomallei. Appl Environ Microbiol (2009) 1.74

In vitro properties of BAL30072, a novel siderophore sulfactam with activity against multiresistant gram-negative bacilli. Antimicrob Agents Chemother (2010) 1.68

Within-host evolution of Burkholderia pseudomallei over a twelve-year chronic carriage infection. MBio (2013) 1.66

Overexpression of resistance-nodulation-cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii. Antimicrob Agents Chemother (2010) 1.58

Mechanisms of antibiotic resistance in Burkholderia pseudomallei: implications for treatment of melioidosis. Future Microbiol (2012) 1.52

The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux system. Antimicrob Agents Chemother (2010) 1.50

Evolution of Burkholderia pseudomallei in recurrent melioidosis. PLoS One (2012) 1.43

Discovery and characterization of inhibitors of Pseudomonas aeruginosa type III secretion. Antimicrob Agents Chemother (2010) 1.38

Molecular basis of rare aminoglycoside susceptibility and pathogenesis of Burkholderia pseudomallei clinical isolates from Thailand. PLoS Negl Trop Dis (2009) 1.34

Control of quorum sensing by a Burkholderia pseudomallei multidrug efflux pump. J Bacteriol (2007) 1.06

Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for human respiratory epithelial cells. BMC Microbiol (2010) 0.95

Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa. Front Microbiol (2015) 0.94

Efflux pump-mediated drug resistance in Burkholderia. Front Microbiol (2015) 0.91

Differing ability to transport nonmetal substrates by two RND-type metal exporters. FEMS Microbiol Lett (2010) 0.90

The BpeEF-OprC efflux pump is responsible for widespread trimethoprim resistance in clinical and environmental Burkholderia pseudomallei isolates. Antimicrob Agents Chemother (2013) 0.87

Nitric oxide-dependent killing of aerobic, anaerobic and persistent Burkholderia pseudomallei. Nitric Oxide (2012) 0.87

Involvement of the efflux pumps in chloramphenicol selected strains of Burkholderia thailandensis: proteomic and mechanistic evidence. PLoS One (2011) 0.86

Triclosan can select for an AdeIJK-overexpressing mutant of Acinetobacter baumannii ATCC 17978 that displays reduced susceptibility to multiple antibiotics. Antimicrob Agents Chemother (2014) 0.86

Exploring the HME and HAE1 efflux systems in the genus Burkholderia. BMC Evol Biol (2010) 0.85

When it comes to drug discovery not all Gram-negative bacterial biodefence pathogens are created equal: Burkholderia pseudomallei is different. Microb Biotechnol (2012) 0.79

Interplay between three RND efflux pumps in doxycycline-selected strains of Burkholderia thailandensis. PLoS One (2013) 0.79

Antibiotic resistance in Burkholderia species. Drug Resist Updat (2016) 0.78

Resistance-Nodulation-Division Multidrug Efflux Pumps in Gram-Negative Bacteria: Role in Virulence. Antibiotics (Basel) (2013) 0.77

Nitric oxide from IFNγ-primed macrophages modulates the antimicrobial activity of β-lactams against the intracellular pathogens Burkholderia pseudomallei and Nontyphoidal Salmonella. PLoS Negl Trop Dis (2014) 0.76

Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients. MBio (2017) 0.75

Development and characterization of a Pseudomonas aeruginosa in vitro coupled transcription-translation assay system for evaluation of translation inhibitors. J Microbiol Methods (2012) 0.75

Articles cited by this

A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene (1998) 15.03

Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev (2005) 14.71

Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa. Gene (1994) 6.75

A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J Microbiol Methods (2005) 6.02

Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy. Antimicrob Agents Chemother (2001) 5.23

Multidrug-resistance efflux pumps - not just for resistance. Nat Rev Microbiol (2006) 5.18

A Tn7-based broad-range bacterial cloning and expression system. Nat Methods (2005) 4.66

Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei. Antimicrob Agents Chemother (1999) 4.29

Coordinate regulation of bacterial virulence genes by a novel adenylate cyclase-dependent signaling pathway. Dev Cell (2003) 3.72

mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat Protoc (2006) 3.19

Aminoglycoside efflux in Pseudomonas aeruginosa: involvement of novel outer membrane proteins. Antimicrob Agents Chemother (2003) 1.59

BpeAB-OprB, a multidrug efflux pump in Burkholderia pseudomallei. Antimicrob Agents Chemother (2004) 1.58

Substrate-dependent utilization of OprM or OpmH by the Pseudomonas aeruginosa MexJK efflux pump. Antimicrob Agents Chemother (2005) 1.29

Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia). J Clin Invest (2004) 1.18

Articles by these authors

A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J Microbiol Methods (2005) 6.02

A Tn7-based broad-range bacterial cloning and expression system. Nat Methods (2005) 4.66

mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat Protoc (2006) 3.19

An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants. BMC Microbiol (2005) 2.53

Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Appl Environ Microbiol (2007) 2.40

Management of accidental laboratory exposure to Burkholderia pseudomallei and B. mallei. Emerg Infect Dis (2008) 2.24

PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria. Appl Environ Microbiol (2008) 2.16

Two aerobic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosa. Mol Microbiol (2006) 2.05

The MexJK efflux pump of Pseudomonas aeruginosa requires OprM for antibiotic efflux but not for efflux of triclosan. J Bacteriol (2002) 1.76

Versatile dual-technology system for markerless allele replacement in Burkholderia pseudomallei. Appl Environ Microbiol (2009) 1.74

Bacterial resistance to antibiotics: active efflux and reduced uptake. Adv Drug Deliv Rev (2005) 1.71

mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344. Nat Protoc (2006) 1.58

The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux system. Antimicrob Agents Chemother (2010) 1.50

Pseudomonas aeruginosa-plant root interactions. Pathogenicity, biofilm formation, and root exudation. Plant Physiol (2003) 1.50

Workshop on treatment of and postexposure prophylaxis for Burkholderia pseudomallei and B. mallei Infection, 2010. Emerg Infect Dis (2012) 1.48

Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1. Microbiology (2003) 1.46

Present and future therapeutic strategies for melioidosis and glanders. Expert Rev Anti Infect Ther (2010) 1.45

Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI. Mol Microbiol (2004) 1.40

Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins. J Bacteriol (2007) 1.36

Single-copy chromosomal integration systems for Francisella tularensis. Microbiology (2009) 1.34

Molecular basis of rare aminoglycoside susceptibility and pathogenesis of Burkholderia pseudomallei clinical isolates from Thailand. PLoS Negl Trop Dis (2009) 1.34

Molecular basis of azithromycin-resistant Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother (2005) 1.30

Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei. Proc Natl Acad Sci U S A (2011) 1.29

Substrate-dependent utilization of OprM or OpmH by the Pseudomonas aeruginosa MexJK efflux pump. Antimicrob Agents Chemother (2005) 1.29

The BpsIR quorum-sensing system of Burkholderia pseudomallei. J Bacteriol (2005) 1.27

A Burkholderia pseudomallei deltapurM mutant is avirulent in immunocompetent and immunodeficient animals: candidate strain for exclusion from select-agent lists. Infect Immun (2010) 1.26

Molecular Investigations of PenA-mediated β-lactam Resistance in Burkholderia pseudomallei. Front Microbiol (2011) 1.25

mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320. Nat Protoc (2006) 1.20

High-level triclosan resistance in Pseudomonas aeruginosa is solely a result of efflux. Am J Infect Control (2003) 1.18

Beta-ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths. Microbiology (2002) 1.16

In vivo Bioluminescence Imaging of Burkholderia mallei Respiratory Infection and Treatment in the Mouse Model. Front Microbiol (2011) 1.14

Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer. J Bacteriol (2002) 1.13

Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival. J Proteomics (2011) 1.13

Benchtop and microcentrifuge preparation of Pseudomonas aeruginosa competent cells. Biotechniques (2002) 1.09

Immunotherapy markedly increases the effectiveness of antimicrobial therapy for treatment of Burkholderia pseudomallei infection. Antimicrob Agents Chemother (2010) 1.08

Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia. Trans R Soc Trop Med Hyg (2008) 1.08

In vivo Himar1 transposon mutagenesis of Burkholderia pseudomallei. Appl Environ Microbiol (2008) 1.08

Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration. EMBO J (2007) 1.07

Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii. J Microbiol Methods (2010) 1.02

In vitro activity of BAL30072 against Burkholderia pseudomallei. Int J Antimicrob Agents (2011) 1.01

A high-throughput, homogeneous, bioluminescent assay for Pseudomonas aeruginosa gyrase inhibitors and other DNA-damaging agents. J Biomol Screen (2007) 1.00

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis. Appl Environ Microbiol (2013) 1.00

Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria. Appl Environ Microbiol (2012) 1.00

Does microbicide use in consumer products promote antimicrobial resistance? A critical review and recommendations for a cohesive approach to risk assessment. Microb Drug Resist (2013) 0.96

Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders. Front Cell Infect Microbiol (2012) 0.93

Molecular characterization of MexL, the transcriptional repressor of the mexJK multidrug efflux operon in Pseudomonas aeruginosa. Antimicrob Agents Chemother (2005) 0.92

Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp. FEMS Microbiol Lett (2009) 0.92

An improved method for oriT-directed cloning and functionalization of large bacterial genomic regions. Appl Environ Microbiol (2013) 0.90

Genetic characterization of pcpS, encoding the multifunctional phosphopantetheinyl transferase of Pseudomonas aeruginosa. Microbiology (2004) 0.90

The Burkholderia pseudomallei Δasd mutant exhibits attenuated intracellular infectivity and imparts protection against acute inhalation melioidosis in mice. Infect Immun (2011) 0.89

In vitro activity of cethromycin against Burkholderia pseudomallei and investigation of mechanism of resistance. J Antimicrob Chemother (2010) 0.88

Burkholderia pseudomallei known siderophores and hemin uptake are dispensable for lethal murine melioidosis. PLoS Negl Trop Dis (2012) 0.88

The BpeEF-OprC efflux pump is responsible for widespread trimethoprim resistance in clinical and environmental Burkholderia pseudomallei isolates. Antimicrob Agents Chemother (2013) 0.87

The Burkholderia pseudomallei enoyl-acyl carrier protein reductase FabI1 is essential for in vivo growth and is the target of a novel chemotherapeutic with efficacy. Antimicrob Agents Chemother (2013) 0.87

Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI. J Bacteriol (2006) 0.86

Correlates of immune protection following cutaneous immunization with an attenuated Burkholderia pseudomallei vaccine. Infect Immun (2013) 0.86

New insights from the 7th World Melioidosis Congress 2013. Emerg Infect Dis (2014) 0.86

Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation. J Bacteriol (2012) 0.85

Cellular reporter screens for inhibitors of Burkholderia pseudomallei targets in Pseudomonas aeruginosa. Trans R Soc Trop Med Hyg (2008) 0.85

Global gene expression profiles suggest an important role for nutrient acquisition in early pathogenesis in a plant model of Pseudomonas aeruginosa infection. Appl Environ Microbiol (2008) 0.85

Vaccination of ducks with recombinant outer membrane protein (OmpA) and a 41 kDa partial protein (P45N') of Riemerella anatipestifer. Vet Microbiol (2002) 0.85

High-level pacidamycin resistance in Pseudomonas aeruginosa is mediated by an opp oligopeptide permease encoded by the opp-fabI operon. Antimicrob Agents Chemother (2013) 0.84

Substituted diphenyl ethers as a novel chemotherapeutic platform against Burkholderia pseudomallei. Antimicrob Agents Chemother (2013) 0.83

φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. mallei strain infectivity. BMC Microbiol (2012) 0.83

Evidence of MexT-independent overexpression of MexEF-OprN multidrug efflux pump of Pseudomonas aeruginosa in presence of metabolic stress. PLoS One (2011) 0.83

Global transcriptional responses to triclosan exposure in Pseudomonas aeruginosa. Int J Antimicrob Agents (2012) 0.82

A simple method for construction of pir+ Enterobacterial hosts for maintenance of R6K replicon plasmids. BMC Res Notes (2012) 0.81

Development and application of a cellular, gain-of-signal, bioluminescent reporter screen for inhibitors of type II secretion in Pseudomonas aeruginosa and Burkholderia pseudomallei. J Biomol Screen (2011) 0.81

Role of the MexXY multidrug efflux pump in moderate aminoglycoside resistance in Pseudomonas aeruginosa isolates from Pseudomonas mastitis. Microbiol Immunol (2008) 0.81

Crystallization of Pseudomonas aeruginosa AHL synthase LasI using beta-turn crystal engineering. Acta Crystallogr D Biol Crystallogr (2004) 0.80

Choline catabolism, σ⁵⁴ factor and NtrC are required for the full expression of the Pseudomonas aeruginosa phosphorylcholine phosphatase gene. Microbiol Res (2010) 0.80

Functional characterization of MexXY and OpmG in aminoglycoside efflux in Pseudomonas aeruginosa. Southeast Asian J Trop Med Public Health (2008) 0.80

Dissecting novel virulent determinants in the Burkholderia cepacia complex. Virulence (2012) 0.80

Development of an ELISA using a recombinant 41 kDa partial protein (P45N') for the detection of Riemerella anatipestifer infections in ducks. Vet Microbiol (2002) 0.79

Methods for genetic manipulation of Burkholderia gladioli pathovar cocovenenans. BMC Res Notes (2010) 0.78

An improved selective culture medium enhances the isolation of Burkholderia pseudomallei from contaminated specimens. Am J Trop Med Hyg (2013) 0.76

Characterization of d-boroAla as a novel broad-spectrum antibacterial agent targeting d-Ala-d-Ala ligase. Chem Biol Drug Des (2011) 0.75

Polar lipids of Burkholderia pseudomallei induce different host immune responses. PLoS One (2013) 0.75

Absence of Bacteria in the Temporal Arteries of Patients with Giant Cell Arteritis. J Clin Rheumatol (2016) 0.75

Spatial transcriptomes within the Pseudomonas aeruginosa biofilm architecture. Mol Microbiol (2017) 0.75