PubRank

B Witholt

Author PubWeight™ 166.35‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli. Anal Biochem 1976 5.79
2 Industrial biocatalysis today and tomorrow. Nature 2001 4.99
3 Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase. Appl Environ Microbiol 1989 4.45
4 Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli. Nature 1991 4.11
5 Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates. Appl Environ Microbiol 1988 3.46
6 Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10. J Bacteriol 1985 3.37
7 Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10. Appl Environ Microbiol 1985 3.26
8 Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane. J Bacteriol 1983 2.78
9 Genetics of alkane oxidation by Pseudomonas oleovorans. Biodegradation 1994 2.66
10 Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene. J Bacteriol 1989 2.59
11 Release of outer membrane fragments from normally growing Escherichia coli. Biochim Biophys Acta 1976 2.51
12 Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA. J Biol Chem 1991 2.43
13 Synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads. Appl Environ Microbiol 1989 2.38
14 The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli. Biochim Biophys Acta 1978 2.37
15 Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints. J Mol Biol 1990 2.21
16 Refined structure of Escherichia coli heat-labile enterotoxin, a close relative of cholera toxin. J Mol Biol 1993 2.17
17 Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes. Microbiology 2001 2.13
18 Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. Appl Environ Microbiol 2000 2.10
19 Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers. Appl Environ Microbiol 1992 2.07
20 The Pseudomonas oleovorans alkane hydroxylase gene. Sequence and expression. J Biol Chem 1989 1.94
21 Method for isolating mutants overproducing nicotinamide adenine dinucleotide and its precursors. J Bacteriol 1972 1.93
22 The Pseudomonas oleovorans alkBAC operon encodes two structurally related rubredoxins and an aldehyde dehydrogenase. J Biol Chem 1989 1.83
23 Some characteristics of the outer membrane material released by growing enterotoxigenic Escherichia coli. Infect Immun 1980 1.70
24 Preferential release of new outer membrane fragments by exponentially growing Escherichia coli. Biochim Biophys Acta 1978 1.69
25 Purification and characterization of a bacterial dehalogenase with activity toward halogenated alkanes, alcohols and ethers. Eur J Biochem 1988 1.65
26 Identification of granule-bound starch synthase in potato tubers. Plant Physiol 1986 1.65
27 Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli. J Biol Chem 1987 1.64
28 Towards a biocatalyst for (S)-styrene oxide production: characterization of the styrene degradation pathway of Pseudomonas sp. strain VLB120. Appl Environ Microbiol 1998 1.61
29 Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains. Environ Microbiol 1999 1.61
30 Occurrence, synthesis and medical application of bacterial polyhydroxyalkanoate. Adv Drug Deliv Rev 2001 1.57
31 DNA sequence determination and functional characterization of the OCT-plasmid-encoded alkJKL genes of Pseudomonas oleovorans. Mol Microbiol 1992 1.55
32 Isolation of the membranes of an enterotoxigenic strain of Escherichia coli and distribution of enterotoxin activity in different subcellular fractions. Biochim Biophys Acta 1978 1.46
33 Assembly pathway of newly synthesized LamB protein an outer membrane protein of Escherichia coli K-12. J Mol Biol 1984 1.45
34 Alkane utilization in Pseudomonas oleovorans. Structure and function of the regulatory locus alkR. J Biol Chem 1988 1.43
35 PhaF, a polyhydroxyalkanoate-granule-associated protein of Pseudomonas oleovorans GPo1 involved in the regulatory expression system for pha genes. J Bacteriol 1999 1.40
36 Characterization of a low density cytoplasmic membrane subfraction isolated from Escherichia coli. Biochim Biophys Acta 1975 1.40
37 Outer-membrane vesicles released by normally growing Escherichia coli contain very little lipoprotein. Eur J Biochem 1981 1.39
38 Gene cloning and characterization of multiple alkane hydroxylase systems in Rhodococcus strains Q15 and NRRL B-16531. Appl Environ Microbiol 2002 1.38
39 Degradation of n-haloalkanes and alpha, omega-dihaloalkanes by wild-type and mutants of Acinetobacter sp. strain GJ70. Appl Environ Microbiol 1987 1.37
40 K88-mediated binding of Escherichia coli outer membrane fragments to porcine intestinal epithelial cell brush borders. Infect Immun 1981 1.35
41 Immunological comparison of the starch branching enzymes from potato tubers and maize kernels. Plant Physiol 1989 1.33
42 Topology of the membrane-bound alkane hydroxylase of Pseudomonas oleovorans. J Biol Chem 1992 1.33
43 How does lysozyme penetrate through the bacterial outer membrane? Biochim Biophys Acta 1976 1.28
44 Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis. Curr Opin Biotechnol 2001 1.26
45 Bioconversion of n-octane to octanoic acid by a recombinant Escherichia coli cultured in a two-liquid phase bioreactor. Biotechnology (N Y) 1991 1.25
46 Polymerase C1 levels and poly(R-3-hydroxyalkanoate) synthesis in wild-type and recombinant Pseudomonas strains. J Bacteriol 1997 1.24
47 Kinetics of synthesis, processing, and membrane transport of heat-labile enterotoxin, a periplasmic protein in Escherichia coli. J Biol Chem 1984 1.23
48 Synthesis of 1,2-Epoxyoctane by Pseudomonas oleovorans During Growth in a Two-Phase System Containing High Concentrations of 1-Octene. Appl Environ Microbiol 1981 1.22
49 Factors involved in the regulatory network of polyhydroxyalkanoate metabolism. J Biotechnol 2001 1.16
50 New alkane-responsive expression vectors for Escherichia coli and pseudomonas. Plasmid 2001 1.14
51 Structure of the Pseudomonas putida alkBAC operon. Identification of transcription and translation products. J Biol Chem 1987 1.14
52 Identification of different forms of the murein-bound lipoprotein found in isolated outer membranes of Escherichia coli. Eur J Biochem 1981 1.14
53 Growth on octane alters the membrane lipid fatty acids of Pseudomonas oleovorans due to the induction of alkB and synthesis of octanol. J Bacteriol 1995 1.13
54 Production of enantiopure styrene oxide by recombinant Escherichia coli synthesizing a two-component styrene monooxygenase. Biotechnol Bioeng 2000 1.10
55 Nature of the regions involved in the insertion of newly synthesized protein into the outer membrane of Escherichia coli. Biochim Biophys Acta 1979 1.09
56 Heat-labile enterotoxin in Escherichia coli. Kinetics of association of subunits into periplasmic holotoxin. J Biol Chem 1985 1.09
57 Biosynthesis of synthons in two-liquid-phase media. Biotechnol Bioeng 1996 1.08
58 Conversion of pBR322-based plasmids into broad-host-range vectors by using the Tn3 transposition mechanism. J Bacteriol 1994 1.08
59 Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase. J Biol Chem 1990 1.08
60 Release of outer membrane fragments from wild-type Escherichia coli and from several E. coli lipopolysaccharide mutants by EDTA and heat shock treatments. J Bacteriol 1989 1.06
61 TOL plasmid-specified xylene oxygenase is a wide substrate range monooxygenase capable of olefin epoxidation. Enzyme Microb Technol 1994 1.05
62 The PalkBFGHJKL promoter is under carbon catabolite repression control in Pseudomonas oleovorans but not in Escherichia coli alk+ recombinants. J Bacteriol 1999 1.05
63 Insertion of newly synthesized proteins into the outer membrane of Escherichia coli. Biochim Biophys Acta 1978 1.04
64 Effectiveness of orbital shaking for the aeration of suspended bacterial cultures in square-deepwell microtiter plates. Biochem Eng J 2001 1.03
65 Production of Pseudomonas aeruginosa Rhamnolipid Biosurfactants in Heterologous Hosts. Appl Environ Microbiol 1995 1.02
66 Biotransformation of limonene by bacteria, fungi, yeasts, and plants. Appl Microbiol Biotechnol 2003 1.02
67 Production of medium-chain-length poly(3-hydroxyalkanoates) from gluconate by recombinant Escherichia coli. Appl Environ Microbiol 1999 1.01
68 Structural heterogeneity of the cytoplasmic and outer membranes of Escherichia coli. Biochim Biophys Acta 1977 1.01
69 Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments. Plant Mol Biol 1989 1.00
70 Expression, stability and performance of the three-component alkane mono-oxygenase of Pseudomonas oleovorans in Escherichia coli. Eur J Biochem 2000 1.00
71 Xylene monooxygenase catalyzes the multistep oxygenation of toluene and pseudocumene to corresponding alcohols, aldehydes, and acids in Escherichia coli JM101. J Biol Chem 2000 0.99
72 Properties of engineered poly-3-hydroxyalkanoates produced in recombinant Escherichia coli strains. Appl Environ Microbiol 2000 0.98
73 In vitro activities of granule-bound poly[(R)-3-hydroxyalkanoate]polymerase C1 of Pseudomonas oleovorans--development of an activity test for medium-chain-length-poly(3-hydroxyalkanoate) polymerases. Eur J Biochem 1997 0.97
74 Physiological changes and alk gene instability in Pseudomonas oleovorans during induction and expression of alk genes. J Bacteriol 1996 0.97
75 Accumulation of Poly[(R)-3-hydroxyalkanoates] in Pseudomonas oleovorans during growth with octanoate in continuous culture at different dilution rates. Appl Environ Microbiol 2000 0.96
76 The alkane oxidation system of Pseudomonas oleovorans: induction of the alk genes in Escherichia coli W3110 (pGEc47) affects membrane biogenesis and results in overexpression of alkane hydroxylase in a distinct cytoplasmic membrane subfraction. Mol Microbiol 1993 0.96
77 Engineering of stable recombinant bacteria for production of chiral medium-chain-length poly-3-hydroxyalkanoates. Appl Environ Microbiol 1999 0.95
78 High-sensitivity detection of newly induced LamB protein on the Escherichia coli cell surface. J Bacteriol 1984 0.95
79 Isolation and characterization of the cis-trans-unsaturated fatty acid isomerase of Pseudomonas oleovorans GPo12. J Bacteriol 1999 0.95
80 Engineering of a stable whole-cell biocatalyst capable of (S)-styrene oxide formation for continuous two-liquid-phase applications. Appl Environ Microbiol 1999 0.94
81 FabG, an NADPH-dependent 3-ketoacyl reductase of Pseudomonas aeruginosa, provides precursors for medium-chain-length poly-3-hydroxyalkanoate biosynthesis in Escherichia coli. J Bacteriol 2000 0.94
82 Biotransformation of D-limonene to (+) trans-carveol by toluene-grown Rhodococcus opacus PWD4 cells. Appl Environ Microbiol 2001 0.92
83 Role of phaD in accumulation of medium-chain-length Poly(3-hydroxyalkanoates) in Pseudomonas oleovorans. Appl Environ Microbiol 2000 0.92
84 Synthesis of alkane hydroxylase of Pseudomonas oleovorans increases the iron requirement of alk+ bacterial strains. Biotechnol Bioeng 1998 0.91
85 Recovery of active medium-chain-length-poly-3-hydroxyalkanoate polymerase from inactive inclusion bodies using ion-exchange resin. Biochem J 2000 0.91
86 Production of microbial polyesters: fermentation and downstream processes. Adv Biochem Eng Biotechnol 2001 0.91
87 Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells. J Bacteriol 1984 0.91
88 The effect of osmotic shock on the accessibility of the murein layer of exponentially growing Escherichia coli to lysozyme. Biochim Biophys Acta 1978 0.91
89 Accumulation of poly[(R)-3-hydroxyalkanoates] in Pseudomonas oleovorans during growth in batch and chemostat culture with different carbon sources. Biotechnol Bioeng 2001 0.90
90 Conversion of free lipoprotein to the murein-bound form. Eur J Biochem 1981 0.89
91 Heat-labile enterotoxin of Escherichia coli. Characterization of different crystal forms. J Biol Chem 1985 0.89
92 Selective measurement of starch synthesizing enzymes in permeabilized potato tuber slices. Plant Physiol 1990 0.87
93 Closed-loop control of bacterial high-cell-density fed-batch cultures: production of mcl-PHAs by Pseudomonas putida KT2442 under single-substrate and cofeeding conditions. Biotechnol Bioeng 1999 0.87
94 Attachment of lipoprotein to the murein of Escherichia coli. Eur J Biochem 1982 0.86
95 Biocatalyst engineering by assembly of fatty acid transport and oxidation activities for In vivo application of cytochrome P-450BM-3 monooxygenase. Appl Environ Microbiol 1998 0.86
96 A highly efficient procedure for the quantitative formation of intact and viable lysozyme spheroplasts from Escherichia coli. Anal Biochem 1987 0.86
97 Continuous bioconversion of n-octane to octanoic acid by recombinant Escherichia coli (alk(+)) growing in a two-liquid-phase Chemostat. Biotechnol Bioeng 1993 0.86
98 Distribution of newly synthesized lipoprotein over the outer membrane and the peptidoglycan sacculus of an Escherichia coli lac-lpp strain. J Bacteriol 1987 0.86
99 Overproduction of a foreign membrane protein in Escherichia coli stimulates and depends on phospholipid synthesis. Eur J Biochem 1996 0.85
100 Inactivation of isocitrate lyase leads to increased production of medium-chain-length poly(3-hydroxyalkanoates) in Pseudomonas putida. Appl Environ Microbiol 2000 0.85
101 Induction kinetics and cell surface distribution of Escherichia coli lipoprotein under lac promoter control. J Bacteriol 1986 0.84
102 High cell density cultivation of Pseudomonas oleovorans: growth and production of poly (3-hydroxyalkanoates) in two-liquid phase batch and fed-batch systems. Biotechnol Bioeng 1993 0.84
103 Preparation of (R)- and (S)-N-protected 3-hydroxypyrrolidines by hydroxylation with Sphingomonas sp. HXN-200, a highly active, regio- and stereoselective, and easy to handle biocatalyst. J Org Chem 2001 0.84
104 An in vitro system to study interactions between bacteria and epithelial cells at the molecular level. J Gen Microbiol 1983 0.84
105 Development of an improved in vitro activity assay for medium chain length PHA polymerases based on CoenzymeA release measurements. J Microbiol Methods 2000 0.83
106 Solubilization of the overexpressed integral membrane protein alkane monooxygenase of the recombinant Escherichia coli W3110[pGEc47]. Biochim Biophys Acta 1994 0.83
107 Developments toward large-scale bacterial bioprocesses in the presence of bulk amounts of organic solvents. Extremophiles 1998 0.82
108 Controlled regioselectivity of fatty acid oxidation by whole cells producing cytochrome P450BM-3 monooxygenase under varied dissolved oxygen concentrations. Biotechnol Bioeng 1999 0.81
109 Co-translational insertion of envelope proteins: theoretical consideration and implications. Ann Microbiol (Paris) 1982 0.80
110 Growth characteristics of Escherichia coli HB101[pGEc47] on defined medium. Biotechnol Bioeng 1998 0.80
111 The AlkB monooxygenase of Pseudomonas oleovorans--synthesis, stability and level in recombinant Escherichia coli and the native host. Eur J Biochem 1997 0.80
112 Determinants for overproduction of the Pseudomonas oleovorans cytoplasmic membrane protein alkane hydroxylase in alk+ Escherichia coli W3110. J Bacteriol 1997 0.79
113 Fusion of small unilamellar vesicles with viable EDTA-treated Escherichia coli cells. J Bacteriol 1989 0.79
114 Preparation of (S)-N-substituted 4-hydroxy-pyrrolidin-2-ones by regio- and stereoselective hydroxylation with Sphingomonas sp. HXN-200. Org Lett 2000 0.78
115 Efficient transformation of potato (Solanum tuberosum L.) using a binary vector in Agrobacterium rhizogenes. Theor Appl Genet 1989 0.77
116 In situ hybridization to somatic metaphase chromosomes of potato. Theor Appl Genet 1988 0.77
117 Two-stage continuous process development for the production of medium-chain-length poly(3-hydroxyalkanoates). Biotechnol Bioeng 2001 0.77
118 Multioscillator fluorescence depolarization. Anisotropy of dye binding. Biochemistry 1970 0.77
119 Single-stranded circular DNA generated from broad host range plasmid R1162 and its derivatives. Plasmid 1989 0.77
120 Kinetics of induction of the lamB-gene product in Escherichia coli: appearance of the newly synthesized protein in the cell envelope. Ann Microbiol (Paris) 1982 0.77
121 An integrated process for the production of toxic catechols from toxic phenols based on a designer biocatalyst. Biotechnol Bioeng 1999 0.76
122 Biotransformation of octane by E. coli HB101[pGEc47] on defined medium: octanoate production and product inhibition. Biotechnol Bioeng 1998 0.76
123 p-Hydroxyphenylacetic Acid Metabolism in Pseudomonas putida F6. J Bacteriol 2001 0.75
124 Proline transport activity in Escherichia coli membrane vesicles of different buoyant densities. Biochim Biophys Acta 1977 0.75
125 Expression and inheritance of inserted markers in binary vector carrying Agrobacterium rhizogenes-transformed potato (Solanum tuberosum L.). Theor Appl Genet 1989 0.75
126 Chromatography of cell suspensions: a simple method for the determination of pyridine nucleotides. Anal Biochem 1974 0.75
127 Inhibition of microbial cholesterol oxidases by dimethylmorpholines. J Steroid Biochem 1990 0.75
128 Developments in biotechnology of relevance to drinking water preparation. Sci Total Environ 1985 0.75