Published in Biochemistry on October 31, 1989
Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli. Appl Environ Microbiol (2000) 1.20
Trehalose synthesis by sequential reactions of recombinant maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase from Brevibacterium helvolum. Appl Environ Microbiol (2000) 0.88
Synthetic metabolic pipelines. Nat Biotechnol (2009) 0.86
Construction of engineered bifunctional enzymes and their overproduction in Aspergillus niger for improved enzymatic tools to degrade agricultural by-products. Appl Environ Microbiol (2005) 0.82
NAD(H) recycling activity of an engineered bifunctional enzyme galactose dehydrogenase/lactate dehydrogenase. Int J Biol Sci (2006) 0.80
An artificial transport metabolon facilitates improved substrate utilization in yeast. Nat Chem Biol (2017) 0.75
High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product. Gene (1989) 2.63
Molecularly imprinted polymers and their use in biomimetic sensors. Chem Rev (2000) 2.08
Drug assay using antibody mimics made by molecular imprinting. Nature (1993) 2.04
Penicillin acylase from E. coli: unique gene-protein relation. Nucleic Acids Res (1986) 1.82
General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide. Biochem J (1972) 1.81
Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase. Appl Environ Microbiol (1996) 1.69
Metabolic compartmentation: symbiotic, organellar, multienzymic, and microenvironmental. Annu Rev Microbiol (1974) 1.67
Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production. Nat Biotechnol (1997) 1.63
Matrix-bound enzymes. II. Studies on a matrix-bound two-enzyme-system. Acta Chem Scand (1970) 1.52
Affinity chromatography and binding studies on immobilized 5'-monophosphate and adenosine 2',5'-bisphosphate of nicotinamide nucleotide transhydrogenase from Pseudomonas aeruginosa. Eur J Biochem (1976) 1.52
Preparation of a NAD(H)-polymer matrix showing coenzyme function of the bound pyridine nucleotide. Biotechnol Bioeng (1971) 1.49
Expression of Pseudomonas fluorescens D-galactose dehydrogenase in E. coli. Gene (1981) 1.49
Flame retardants in indoor air at an electronics recycling plant and at other work environments. Environ Sci Technol (2001) 1.40
Enzymes bound to artificial matrixes. Sci Am (1971) 1.38
Studies on a matrix-bound three-enzyme system. Biochim Biophys Acta (1971) 1.32
Alteration of ribosomal protein L6 in gentamicin-resistant strains of Escherichia coli. Effects on fidelity of protein synthesis. Biochemistry (1979) 1.30
Entrapment of animal cells for production of monoclonal antibodies and other biomolecules. Nature (1983) 1.29
Separation of the isoenzymes of lactate dehydrogenase by affinity chromatography using an immobilized AMP-analogue. FEBS Lett (1973) 1.28
Preparation and application of polymer-entrapped enzymes and microorganisms in microbial transformation processes with special reference to steroid 11-beta-hydroxylation and delta-dehydrogenation. Biotechnol Bioeng (1970) 1.21
Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals. Trends Biotechnol (2001) 1.19
A new immobilized NAD+ analogue, its application in affinity chromatography and as a functioning coenzyme. Eur J Biochem (1973) 1.18
An immobilized three-enzyme system: a model for microenvironmental compartmentation in mitochondria. Proc Natl Acad Sci U S A (1973) 1.18
Cloning and characterization of baker's yeast alpha-glucosidase: over-expression in a yeast strain devoid of vacuolar proteinases. Yeast (1989) 1.15
The microbe thermistor. Nature (1977) 1.12
Separation of amino acids, peptides and proteins on molecularly imprinted stationary phases. J Chromatogr A (1995) 1.08
Alteration of ribosomal protein L6 in mutants of Escherichia coli resistant to gentamicin. Mol Gen Genet (1977) 1.06
Formation of proinsulin by immobilized Bacillus subtilis. Nature (1983) 1.04
Selection of cadmium specific hexapeptides and their expression as OmpA fusion proteins in Escherichia coli. Protein Eng (1998) 1.04
Use of flow field-flow fractionation for the rapid quantitation of ribosome and ribosomal subunits in Escherichia coli at different protein production conditions. Biotechnol Bioeng (1997) 1.04
Immobilized coenzymes. Methods Enzymol (1976) 1.03
Reversible and specific interaction of dehydrogenases with a coenzyme-coated surface continuously monitored with a reflectometer. Anal Biochem (1986) 1.02
Affinity precipitation of enzymes. FEBS Lett (1979) 1.01
Entrapment of enzymes and microorganisms in synthetic cross-linked polymers and their application in column techniques. Acta Chem Scand (1966) 1.01
Alanyl-tRNA synthetase of Escherichia coli: genetic analysis of the structural gene and of suppressor mutations. Mol Gen Genet (1974) 1.00
Affinity chromatography of enzymes on an AMP-analogue: Specific elution of dehydrogenases from a general ligand. FEBS Lett (1972) 0.99
Some applications of insolubilised cofactors to the purification of pyridine nucleotide-dependent dehydrogenases. Biochem Biophys Res Commun (1972) 0.99
The expression in E. coli of a polymeric gene coding for an esterase mimic catalyzing the hydrolysis of p-nitrophenyl esters. FEBS Lett (1987) 0.97
Preparation of an alcohol-dehydrogenase--NAD(H)--sepharose complex showing no requirement of soluble coenzyme for its activity. Eur J Biochem (1975) 0.97
AMP and NAD as "General Ligands". Methods Enzymol (1974) 0.96
Coconut and Salmonella infection. Appl Microbiol (1967) 0.96
Assay procedures for immobilized enzymes. Methods Enzymol (1976) 0.96
Characterization of an artificial bifunctional enzyme, beta-galactosidase/galactokinase, prepared by gene fusion. Eur J Biochem (1987) 0.96
The use of bead polymerization of acrylic monomers for immobilization of enzymes. Biochim Biophys Acta (1972) 0.96
Preparation and kinetic characterization of a fusion protein of yeast mitochondrial citrate synthase and malate dehydrogenase. Biochemistry (1994) 0.95
Characterization of a D-amino acid oxidase with high activity against cephalosporin C from the yeast Trigonopsis variabilis. Biochem Int (1990) 0.95
Biospecific affinity chromatography in aqueous-organic cosolvent mixtures. The effect of ethylene glycol on the binding of lactate dehydrogenase to an immobilised-AMP analogue. Eur J Biochem (1975) 0.94
p-Toluenesulfonyl chloride as an activating agent of agarose for the preparation of immobilized affinity ligands and proteins. Eur J Biochem (1980) 0.93
Cloning and nucleotide sequence of heavy- and light-chain cDNAs from a creatine-kinase-specific monoclonal antibody. Gene (1987) 0.92
Immobilization of enzymes and affinity ligands to various hydroxyl group carrying supports using highly reactive sulfonyl chlorides. Biochem Biophys Res Commun (1981) 0.92
Synthesis and catalysis by molecularly imprinted materials. Curr Opin Chem Biol (1999) 0.92
Immobilization of ligands with organic sulfonyl chlorides. Methods Enzymol (1984) 0.92
Imprinted polymer-based sensor system for herbicides using differential-pulse voltammetry on screen-printed electrodes. Anal Chem (1999) 0.92
Purification and characterization of chicken liver alcohol dehydrogenase. FEBS Lett (1978) 0.91
Control of formation of active soluble or inactive insoluble baker's yeast alpha-glucosidase PI in Escherichia coli by induction and growth conditions. Mol Gen Genet (1989) 0.91
Redesign of the coenzyme specificity in L-lactate dehydrogenase from bacillus stearothermophilus using site-directed mutagenesis and media engineering. Protein Eng (1999) 0.90
Preparation of immobilized animal cells. FEBS Lett (1980) 0.90
Metal affinity precipitation of proteins carrying genetically attached polyhistidine affinity tails. Eur J Biochem (1991) 0.90
RNA overproducing revertants of an alanyl-tRNA synthetase mutant of Escherichia coli. Mol Gen Genet (1972) 0.90
Multistep enzyme systems. Methods Enzymol (1976) 0.90
Entrapment of animal cells. Methods Enzymol (1987) 0.89
Recycling by a second enzyme of NAD covalently bound to alcohol dehydrogenase. FEBS Lett (1979) 0.89
Plastic antibodies: developments and applications. Trends Biotechnol (1998) 0.89
Determination of dissociation constants for binary dehydrogenase-coenzyme complexes by (bio)affinity chromatography on an immobilized AMP-analogue. Anal Biochem (1976) 0.88
Assay system for the herbicide 2,4-dichlorophenoxyacetic Acid using a molecularly imprinted polymer as an artificial recognition element. Anal Chem (1998) 0.88
Preparation of analogues of ATP, ADP and AMP suitable for binding to matrices and the enzymic interconversion of ATP and ADP in solid phase. Eur J Biochem (1975) 0.88
Reconstitution of functionally active antibody directed against creatine kinase from separately expressed heavy and light chains in non-lymphoid cells. Gene (1987) 0.87
Towards the development of molecularly imprinted artificial receptors for the screening of estrogenic chemicals. Analyst (2001) 0.87
Covalent binding of an NAD analogue to liver alcohol dehydrogenase resulting in an enzyme-coenzyme complex not requiring exogenous coenzyme for activity. Eur J Biochem (1978) 0.87
Studies on pH-activity profiles of an immobilized two-enzyme system. Biochim Biophys Acta (1972) 0.87
Effects of subunit interactions on the activity of lactate dehydrogenase studied in immobilized enzyme systems. Biochemistry (1976) 0.87
Separation of isozymes of horse liver alcohol dehydrogenase and purification of the enzyme by affinity chromatography on an immobilized AMP-analogue. Biochim Biophys Acta (1974) 0.87
Towards artificial antibodies prepared by molecular imprinting. Clin Chem (1996) 0.86
Suppression of temperature-sensitive aminoacyl-tRNA synthetase mutations by ribosomal mutations: a possible mechanism. Mol Gen Genet (1976) 0.86
Physical and kinetic effects on induction of various linker regions in beta-galactosidase/galactose dehydrogenase fusion enzymes. Biochim Biophys Acta (1996) 0.86
High performance liquid affinity chromatography (HPLAC) and its application to the separation of enzymes and antigens. FEBS Lett (1978) 0.86
Multienzyme systems obtained by gene fusion. Trends Biotechnol (1991) 0.85
An approach towards surface imprinting using the enzyme ribonuclease A. J Mol Recognit (1995) 0.85
Immobilized model systems of enzyme sequences. Curr Top Cell Regul (1978) 0.85
A new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene. Gene (1988) 0.85
Immobilized coenzymes in general ligand affinity chromatography and their use as active coenzymes. Adv Enzymol Relat Areas Mol Biol (1978) 0.85
New configurations and applications of molecularly imprinted polymers. J Chromatogr A (2000) 0.85
Molecularly imprinted polymer beads: suspension polymerization using a liquid perfluorocarbon as the dispersing phase. Anal Chem (1996) 0.84
Synthesis of the disaccharide 6-O-beta-D-galactopyranosyl-2-acetamido-2-deoxy-D-galactose using immobilized beta-galactosidase. Biochem Biophys Res Commun (1984) 0.84
Novel affinity techniques. Biochem Soc Trans (1981) 0.84
Mimics of the binding sites of opioid receptors obtained by molecular imprinting of enkephalin and morphine. Proc Natl Acad Sci U S A (1995) 0.84
Enantiomeric resolution on molecularly imprinted polymers prepared with only non-covalent and non-ionic interactions. J Chromatogr (1990) 0.84
Lack of accumulation of unusual guanosine nucleotides upon amino acid starvation of two eukaryotic organisms. Biochim Biophys Acta (1973) 0.83
Determination of glucose, urea and penicillin using enzyme--pH-electrodes. Biochim Biophys Acta (1973) 0.83
Continuous regeneration of NAD(P)+ by flavins covalently bound to sepharose. Biotechnol Bioeng (1976) 0.83
Studies on lichen enzymes. Purification and properties of an orsellinate depside hydrolase obtained from Lasallia pustulata. Eur J Biochem (1971) 0.83