Published in EMBO J on June 01, 1989
Aspartic acid-96 is the internal proton donor in the reprotonation of the Schiff base of bacteriorhodopsin. Proc Natl Acad Sci U S A (1989) 3.06
Substitution of amino acids Asp-85, Asp-212, and Arg-82 in bacteriorhodopsin affects the proton release phase of the pump and the pK of the Schiff base. Proc Natl Acad Sci U S A (1990) 2.80
Time-resolved X-ray diffraction study of structural changes associated with the photocycle of bacteriorhodopsin. EMBO J (1991) 2.54
A defective proton pump, point-mutated bacteriorhodopsin Asp96----Asn is fully reactivated by azide. EMBO J (1989) 2.53
Simultaneous monitoring of light-induced changes in protein side-group protonation, chromophore isomerization, and backbone motion of bacteriorhodopsin by time-resolved Fourier-transform infrared spectroscopy. Proc Natl Acad Sci U S A (1990) 2.38
Voltage dependence of proton pumping by bacteriorhodopsin is regulated by the voltage-sensitive ratio of M1 to M2. Biophys J (1998) 2.32
Thermodynamic stability of water molecules in the bacteriorhodopsin proton channel: a molecular dynamics free energy perturbation study. Biophys J (1996) 2.30
Protonation state of Asp (Glu)-85 regulates the purple-to-blue transition in bacteriorhodopsin mutants Arg-82----Ala and Asp-85----Glu: the blue form is inactive in proton translocation. Proc Natl Acad Sci U S A (1990) 1.94
Microbial and animal rhodopsins: structures, functions, and molecular mechanisms. Chem Rev (2013) 1.93
Surface-bound optical probes monitor protein translocation and surface potential changes during the bacteriorhodopsin photocycle. Proc Natl Acad Sci U S A (1992) 1.89
Light-driven proton or chloride pumping by halorhodopsin. Proc Natl Acad Sci U S A (1993) 1.84
Protein dynamics in the bacteriorhodopsin photocycle: submillisecond Fourier transform infrared spectra of the L, M, and N photointermediates. Proc Natl Acad Sci U S A (1991) 1.71
Replacement of leucine-93 by alanine or threonine slows down the decay of the N and O intermediates in the photocycle of bacteriorhodopsin: implications for proton uptake and 13-cis-retinal----all-trans-retinal reisomerization. Proc Natl Acad Sci U S A (1991) 1.69
Inversion of proton translocation in bacteriorhodopsin mutants D85N, D85T, and D85,96N. Biophys J (1994) 1.63
Stability of bacteriorhodopsin alpha-helices and loops analyzed by single-molecule force spectroscopy. Biophys J (2002) 1.60
Removal of the transducer protein from sensory rhodopsin I exposes sites of proton release and uptake during the receptor photocycle. Biophys J (1993) 1.54
Infrared spectroscopic demonstration of a conformational change in bacteriorhodopsin involved in proton pumping. Proc Natl Acad Sci U S A (1991) 1.51
Primary structure of sensory rhodopsin I, a prokaryotic photoreceptor. EMBO J (1989) 1.43
Deriving the intermediate spectra and photocycle kinetics from time-resolved difference spectra of bacteriorhodopsin. The simpler case of the recombinant D96N protein. Biophys J (1993) 1.42
Redshift of the purple membrane absorption band and the deprotonation of tyrosine residues at high pH: Origin of the parallel photocycles of trans-bacteriorhodopsin. Biophys J (1991) 1.40
Structure of the N intermediate of bacteriorhodopsin revealed by x-ray diffraction. Proc Natl Acad Sci U S A (1996) 1.39
Light-induced currents from oriented purple membrane: I. Correlation of the microsecond component (B2) with the L-M photocycle transition. Biophys J (1990) 1.37
Distortions in the photocycle of bacteriorhodopsin at moderate dehydration. Biophys J (1991) 1.34
Two light-transducing membrane proteins: bacteriorhodopsin and the mammalian rhodopsin. Proc Natl Acad Sci U S A (1993) 1.32
pH-induced structural changes in bacteriorhodopsin studied by Fourier transform infrared spectroscopy. Biophys J (1994) 1.31
In situ determination of transient pKa changes of internal amino acids of bacteriorhodopsin by using time-resolved attenuated total reflection Fourier-transform infrared spectroscopy. Proc Natl Acad Sci U S A (1999) 1.28
Study of the photocycle and charge motions of the bacteriorhodopsin mutant D96N. Biophys J (1993) 1.28
Photoreactions of bacteriorhodopsin at acid pH. Biophys J (1989) 1.21
Locations of Arg-82, Asp-85, and Asp-96 in helix C of bacteriorhodopsin relative to the aqueous boundaries. Proc Natl Acad Sci U S A (1991) 1.20
The tertiary structural changes in bacteriorhodopsin occur between M states: X-ray diffraction and Fourier transform infrared spectroscopy. EMBO J (1997) 1.20
Functional interactions in bacteriorhodopsin: a theoretical analysis of retinal hydrogen bonding with water. Biophys J (1995) 1.19
The reaction of hydroxylamine with bacteriorhodopsin studied with mutants that have altered photocycles: selective reactivity of different photointermediates. Proc Natl Acad Sci U S A (1991) 1.17
Experimental evidence for hydrogen-bonded network proton transfer in bacteriorhodopsin shown by Fourier-transform infrared spectroscopy using azide as catalyst. Proc Natl Acad Sci U S A (1995) 1.17
Charge transport by ion translocating membrane proteins on solid supported membranes. Biophys J (1993) 1.15
Intramolecular proton transfer in channelrhodopsins. Biophys J (2013) 1.14
Photoinduced volume changes associated with the early transformations of bacteriorhodopsin: a laser-induced optoacoustic spectroscopy study. Biophys J (1994) 1.13
Chromophore motion during the bacteriorhodopsin photocycle: polarized absorption spectroscopy of bacteriorhodopsin and its M-state in bacteriorhodopsin crystals. EMBO J (1991) 1.12
Charge motions during the photocycle of pharaonis halorhodopsin. Biophys J (2000) 1.06
Different modes of proton translocation by sensory rhodopsin I. EMBO J (1996) 1.05
Protein structural change at the cytoplasmic surface as the cause of cooperativity in the bacteriorhodopsin photocycle. Biophys J (1996) 1.05
Connectivity of the retinal Schiff base to Asp85 and Asp96 during the bacteriorhodopsin photocycle: the local-access model. Biophys J (1998) 1.04
Decoupling of photo- and proton cycle in the Asp85-->Glu mutant of bacteriorhodopsin. EMBO J (1993) 1.02
Bacterioopsin, haloopsin, and sensory opsin I of the halobacterial isolate Halobacterium sp. strain SG1: three new members of a growing family. J Bacteriol (1993) 1.02
Specific arginine and threonine residues control anion binding and transport in the light-driven chloride pump halorhodopsin. EMBO J (1997) 1.01
Chemical reconstitution of a chloride pump inactivated by a single point mutation. EMBO J (1995) 1.01
Electric signals during the bacteriorhodopsin photocycle, determined over a wide pH range. Biophys J (1998) 0.99
Bacteriorhodopsin is involved in halobacterial photoreception. Proc Natl Acad Sci U S A (1993) 0.98
The residues Leu 93 and Asp 96 act independently in the bacteriorhodopsin photocycle: studies with the leu 93-->Ala, Asp 96-->Asn double mutant. Biophys J (1996) 0.95
Correlation between absorption maxima and thermal isomerization rates in bacteriorhodopsin. Biophys J (1991) 0.95
A residue substitution near the beta-ionone ring of the retinal affects the M substates of bacteriorhodopsin. Biophys J (1992) 0.94
Consequences of amino acid insertions and/or deletions in transmembrane helix C of bacteriorhodopsin. Proc Natl Acad Sci U S A (1992) 0.94
Rapid pH change due to bacteriorhodopsin measured with a tin-oxide electrode. Biophys J (1995) 0.93
Intermediate spectra and photocycle kinetics of the Asp96 --> asn mutant bacteriorhodopsin determined by singular value decomposition with self-modeling. Proc Natl Acad Sci U S A (1999) 0.93
Uv-visible spectroscopy of bacteriorhodopsin mutants: substitution of Arg-82, Asp-85, Tyr-185, and Asp-212 results in abnormal light-dark adaptation. Proc Natl Acad Sci U S A (1990) 0.91
Time-resolved absorption and photothermal measurements with sensory rhodopsin I from Halobacterium salinarum. Biophys J (1999) 0.90
The photoreceptor sensory rhodopsin I as a two-photon-driven proton pump. Proc Natl Acad Sci U S A (1995) 0.88
The role of water in the extracellular half channel of bacteriorhodopsin. Biophys J (1997) 0.88
Low temperature FTIR study of the Schiff base reprotonation during the M-to-bR backphotoreaction: Asp 85 reprotonates two distinct types of Schiff base species at different temperatures. Biophys J (1992) 0.85
Conversion of a light-driven proton pump into a light-gated ion channel. Sci Rep (2015) 0.83
External electric control of the proton pumping in bacteriorhodopsin. Eur Biophys J (2006) 0.83
Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants. Biochemistry (2006) 0.82
Photocycle of dried acid purple form of bacteriorhodopsin. Biophys J (2001) 0.82
Deprotonation of D96 in bacteriorhodopsin opens the proton uptake pathway. Structure (2013) 0.81
Voltage dependence of proton pumping by bacteriorhodopsin mutants with altered lifetime of the M intermediate. PLoS One (2013) 0.81
Properties of the stochastic energization-relaxation channel model for vectorial ion transport. Biophys J (2000) 0.81
Recent advances in engineering microbial rhodopsins for optogenetics. Curr Opin Struct Biol (2015) 0.80
Electrooptical measurements on purple membrane containing bacteriorhodopsin mutants. Biophys J (1996) 0.80
High-performance photovoltaic behavior of oriented purple membrane polymer composite films. Biophys J (2003) 0.78
Photocycle of Exiguobacterium sibiricum rhodopsin characterized by low-temperature trapping in the IR and time-resolved studies in the visible. J Phys Chem B (2013) 0.78
Studies of cation binding in ZnCl2-regenerated bacteriorhodopsin by x-ray absorption fine structures: effects of removing water molecules and adding Cl- ions. Biophys J (1997) 0.77
Reversible inhibition of proton release activity and the anesthetic-induced acid-base equilibrium between the 480 and 570 nm forms of bacteriorhodopsin. Biophys J (1996) 0.76
Sequence and intramolecular distance scoring analyses of microbial rhodopsins. F1000Res (2016) 0.75
Influence of the charge at D85 on the initial steps in the photocycle of bacteriorhodopsin. Biophys J (2009) 0.75
Isotope-Labeled Aspartate Sidechain as a Non-Perturbing Infrared Probe: Application to Investigate the Dynamics of a Carboxylate Buried Inside a Protein. Chem Phys Lett (2017) 0.75
Schiff Base Proton Acceptor Assists Photoisomerization of Retinal Chromophores in Bacteriorhodopsin. Biophys J (2017) 0.75
Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. Methods Enzymol (1974) 16.03
Functions of a new photoreceptor membrane. Proc Natl Acad Sci U S A (1973) 13.48
Reversible photolysis of the purple complex in the purple membrane of Halobacterium halobium. Eur J Biochem (1973) 6.31
Vibrational spectroscopy of bacteriorhodopsin mutants: light-driven proton transport involves protonation changes of aspartic acid residues 85, 96, and 212. Biochemistry (1988) 4.93
Aspartic acid substitutions affect proton translocation by bacteriorhodopsin. Proc Natl Acad Sci U S A (1988) 3.13
Light-driven protonation changes of internal aspartic acids of bacteriorhodopsin: an investigation by static and time-resolved infrared difference spectroscopy using [4-13C]aspartic acid labeled purple membrane. Biochemistry (1985) 3.08
Time-resolved photoelectric and absorption signals from oriented purple membranes immobilized in gel. J Biochem Biophys Methods (1985) 2.47
The halo-opsin gene. II. Sequence, primary structure of halorhodopsin and comparison with bacteriorhodopsin. EMBO J (1987) 2.18
Incorporation of bacteriorhodopsin into a bilayer lipid membrane; a photoelectric-spectroscopic study. FEBS Lett (1976) 1.86
Fast stages of photoelectric processes in biological membranes. I. Bacteriorhodopsin. Eur J Biochem (1981) 1.86
The electrical response to light of bacteriorhodopsin in planar membranes. Biophys J (1978) 1.69
Mechanism of generation and regulation of photopotential by bacteriorhodopsin in bimolecular lipid membrane. Biochim Biophys Acta (1978) 1.56
Direct measurement of electric current generation by cytochrome oxidase, H+-ATPase and bacteriorhodopsin. Nature (1974) 1.42
Bacteriorhodopsin: a molecular photoelectric regulator. Quenching of photovoltaic effect of bimolecular lipid membranes containing bacteriorhodopsin by blue light. FEBS Lett (1977) 1.19
Photocurrents induced on black lipid membranes by purple membranes: a method of reconstitution and a kinetic study of the photocurrents. Ann N Y Acad Sci (1980) 1.03
Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. Methods Enzymol (1974) 16.03
Rhodopsin-like protein from the purple membrane of Halobacterium halobium. Nat New Biol (1971) 14.93
Functions of a new photoreceptor membrane. Proc Natl Acad Sci U S A (1973) 13.48
Reversible photolysis of the purple complex in the purple membrane of Halobacterium halobium. Eur J Biochem (1973) 6.31
Unfolding pathways of individual bacteriorhodopsins. Science (2000) 5.08
Structure of the light-driven chloride pump halorhodopsin at 1.8 A resolution. Science (2000) 3.93
Nonlinear electrical effects in lipid bilayer membranes. I. Ion injection. Biophys J (1969) 3.90
Reversible dissociation of the purple complex in bacteriorhodopsin and identification of 13-cis and all-trans-retinal as its chromophores. Eur J Biochem (1973) 3.70
Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin. EMBO J (1993) 3.54
The 'light' and 'medium' subunits of the photosynthetic reaction centre from Rhodopseudomonas viridis: isolation of the genes, nucleotide and amino acid sequence. EMBO J (1986) 3.23
Role of aspartate-96 in proton translocation by bacteriorhodopsin. Proc Natl Acad Sci U S A (1989) 3.22
Morphology, function and isolation of halobacterial flagella. J Mol Biol (1984) 3.00
Influence of membrane thickness and ion concentration on the properties of the gramicidin a channel. Autocorrelation, spectral power density, relaxation and single-channel studies. Biochim Biophys Acta (1977) 2.91
Photolysis of bacterial rhodopsin. Biophys J (1975) 2.59
Time-resolved X-ray diffraction study of structural changes associated with the photocycle of bacteriorhodopsin. EMBO J (1991) 2.54
A defective proton pump, point-mutated bacteriorhodopsin Asp96----Asn is fully reactivated by azide. EMBO J (1989) 2.53
Single-channel parameters of gramicidin A,B, and C. Biochim Biophys Acta (1976) 2.52
Anaerobic growth of halobacteria. Proc Natl Acad Sci U S A (1980) 2.49
Lipid patches in membrane protein oligomers: crystal structure of the bacteriorhodopsin-lipid complex. Proc Natl Acad Sci U S A (1998) 2.48
Proton migration along the membrane surface and retarded surface to bulk transfer. Nature (1994) 2.41
Closing in on bacteriorhodopsin: progress in understanding the molecule. Annu Rev Biophys Biomol Struct (1999) 2.39
Chromophore equilibria in bacteriorhodopsin. Biophys J (1979) 2.37
A unifying concept for ion translocation by retinal proteins. J Bioenerg Biomembr (1992) 2.36
Voltage dependence of proton pumping by bacteriorhodopsin is regulated by the voltage-sensitive ratio of M1 to M2. Biophys J (1998) 2.32
Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1. Genomics (2008) 2.31
Channel formation kinetics of gramicidin A in lipid bilayer membranes. J Membr Biol (1973) 2.25
Biosynthesis of the purple membrane of halobacteria. Angew Chem Int Ed Engl (1976) 2.20
Effects of surface charge on the conductance of the gramicidin channel. Biochim Biophys Acta (1979) 2.19
The halo-opsin gene. II. Sequence, primary structure of halorhodopsin and comparison with bacteriorhodopsin. EMBO J (1987) 2.18
Phototrophic growth of halobacteria and its use for isolation of photosynthetically-deficient mutants. Ann Microbiol (Paris) (1983) 2.11
[The purple membrane from Halobacterium halobium]. Hoppe Seylers Z Physiol Chem (1972) 2.03
Orthorhombic two-dimensional crystal form of purple membrane. Proc Natl Acad Sci U S A (1980) 2.00
Early intermediates in the transport cycle of the neuronal excitatory amino acid carrier EAAC1. J Gen Physiol (2001) 1.97
Lag1p and Lac1p are essential for the Acyl-CoA-dependent ceramide synthase reaction in Saccharomyces cerevisae. Mol Biol Cell (2001) 1.96
Methyl-accepting taxis proteins in Halobacterium halobium. EMBO J (1989) 1.92
Identification of synaptophysin as a hexameric channel protein of the synaptic vesicle membrane. Science (1988) 1.87
Three-dimensional crystals of membrane proteins: bacteriorhodopsin. Proc Natl Acad Sci U S A (1980) 1.87
Reconstitution of bacteriorhodopsin. FEBS Lett (1974) 1.87
Light-dependent reaction of bacteriorhodopsin with hydroxylamine in cell suspensions of Halobacterium halobium: demonstration of an apo-membrane. FEBS Lett (1974) 1.86
Studies on the retinal-protein interaction in bacteriorhodopsin. Eur J Biochem (1977) 1.85
Formation of ion channels by a negatively charged analog of gramicidin A. J Membr Biol (1977) 1.84
Light-driven proton or chloride pumping by halorhodopsin. Proc Natl Acad Sci U S A (1993) 1.84
Early picosecond events in the photocycle of bacteriorhodopsin. Biophys J (1986) 1.82
Dynamics of different functional parts of bacteriorhodopsin: H-2H labeling and neutron scattering. Proc Natl Acad Sci U S A (1998) 1.81
Temperature-dependent properties of gramicidin A channels. Biochim Biophys Acta (1974) 1.81
Relaxation studies of ion transport systems in lipid bilayer membranes. Q Rev Biophys (1981) 1.81
Initial electron-transfer in the reaction center from Rhodobacter sphaeroides. Proc Natl Acad Sci U S A (1990) 1.79
Two pumps, one principle: light-driven ion transport in halobacteria. Trends Biochem Sci (1989) 1.78
Signal formation in the halobacterial photophobic response mediated by a fourth retinal protein (P480). J Mol Biol (1987) 1.74
Pump currents generated by the purified Na+K+-ATPase from kidney on black lipid membranes. EMBO J (1985) 1.68
Light-induced changes of the pH gradient and the membrane potential in H. halobium. FEBS Lett (1976) 1.66
Structure of the gramicidin A channel: discrimination between the piL,D and the beta helix by electrical measurements with lipid bilayer membranes. Proc Natl Acad Sci U S A (1977) 1.66
The photocycle of the chloride pump halorhodopsin. II: Quantum yields and a kinetic model. EMBO J (1985) 1.64
Functional expression of bacteriorhodopsin in oocytes allows direct measurement of voltage dependence of light induced H+ pumping. FEBS Lett (1995) 1.63
Inversion of proton translocation in bacteriorhodopsin mutants D85N, D85T, and D85,96N. Biophys J (1994) 1.63
The primary structure of sensory rhodopsin II: a member of an additional retinal protein subgroup is coexpressed with its transducer, the halobacterial transducer of rhodopsin II. Proc Natl Acad Sci U S A (1995) 1.62
Channelrhodopsins: directly light-gated cation channels. Biochem Soc Trans (2005) 1.59
Three-dimensional structure of halorhodopsin at 7 A resolution. J Mol Biol (1995) 1.57
Chemotaxis and phototaxis require a CheA histidine kinase in the archaeon Halobacterium salinarium. EMBO J (1995) 1.54
Signal transduction in Halobacterium depends on fumarate. EMBO J (1990) 1.52
Isolation and properties of the native chromoprotein halorhodopsin. EMBO J (1983) 1.51
Electrophysiological characterization of specific interactions between bacterial sensory rhodopsins and their transducers. Proc Natl Acad Sci U S A (2001) 1.51
Phosphorylation in halobacterial signal transduction. EMBO J (1995) 1.49
The action of a carbonsuboxide dimerized gramicidin A on lipid bilayer membranes. Biochim Biophys Acta (1977) 1.49
Specificity of the retinal binding site of bacteriorhodopsin: chemical and stereochemical requirements for the binding of retinol and retinal. Biochemistry (1978) 1.49
Light inhibition of respiration in Halobacterium halobium. FEBS Lett (1973) 1.49
Protein conformational changes in the bacteriorhodopsin photocycle. J Mol Biol (1999) 1.45
Bacteriorhodopsin mutants of Halobacterium sp. GRB. I. The 5-bromo-2'-deoxyuridine selection as a method to isolate point mutants in halobacteria. J Biol Chem (1989) 1.44
Quantitative aspects of energy conversion in halobacteria. FEBS Lett (1977) 1.44
Identification of a hydrogen bond in the phe M197-->Tyr mutant reaction center of the photosynthetic purple bacterium Rhodobacter sphaeroides by X-ray crystallography and FTIR spectroscopy. FEBS Lett (1999) 1.43
Purification and properties of two 2-oxoacid:ferredoxin oxidoreductases from Halobacterium halobium. Eur J Biochem (1981) 1.43
Primary structure of sensory rhodopsin I, a prokaryotic photoreceptor. EMBO J (1989) 1.43
Correlation analysis of electrical noise in lipid bilayer membranes: kinetics of gramicidin A channels. J Membr Biol (1975) 1.42
The reaction center-LH1 antenna complex of Rhodobacter sphaeroides contains one PufX molecule which is involved in dimerization of this complex. Biochemistry (1999) 1.38
Tracing the D-pathway in reconstituted site-directed mutants of cytochrome c oxidase from Paracoccus denitrificans. Biochemistry (2000) 1.37
Role of the PufX protein in photosynthetic growth of Rhodobacter sphaeroides. 2. PufX is required for efficient ubiquinone/ubiquinol exchange between the reaction center QB site and the cytochrome bc1 complex. Biochemistry (1995) 1.36
Bacteriorhodopsin as an example of a light-driven proton pump. Angew Chem Int Ed Engl (1976) 1.35
General concept for ion translocation by halobacterial retinal proteins: the isomerization/switch/transfer (IST) model. Biochemistry (1997) 1.35
The methyl-accepting transducer protein HtrI is functionally associated with the photoreceptor sensory rhodopsin I in the archaeon Halobacterium salinarium. EMBO J (1993) 1.34
Potassium uniport and ATP synthesis in Halobacterium halobium. Eur J Biochem (1978) 1.34
Projection structure of halorhodopsin from Halobacterium halobium at 6 A resolution obtained by electron cryo-microscopy. J Mol Biol (1993) 1.34
The effect of protonation and electrical interactions on the stereochemistry of retinal schiff bases. Biophys J (1985) 1.33
D38 is an essential part of the proton translocation pathway in bacteriorhodopsin. Biochemistry (1996) 1.32
[Synthesis of various carboxylic acids by the fatty acid synthetase multienzyme complex of yeast and the explanation for their structure]. Eur J Biochem (1969) 1.32
pH-induced structural changes in bacteriorhodopsin studied by Fourier transform infrared spectroscopy. Biophys J (1994) 1.31
K+-dependence of electrogenic transport by the NaK-ATPase. Biochim Biophys Acta (1998) 1.30
Identification of the retinal-binding protein in halorhodopsin. J Biol Chem (1982) 1.28
In situ determination of transient pKa changes of internal amino acids of bacteriorhodopsin by using time-resolved attenuated total reflection Fourier-transform infrared spectroscopy. Proc Natl Acad Sci U S A (1999) 1.28