Published in PLoS One on April 24, 2015
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol (2016) 2.05
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR. Genome Biol (2016) 0.98
Resources for the design of CRISPR gene editing experiments. Genome Biol (2015) 0.95
Integrin-YAP/TAZ-JNK cascade mediates atheroprotective effect of unidirectional shear flow. Nature (2016) 0.90
Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line. Sci Rep (2015) 0.89
Cornerstones of CRISPR-Cas in drug discovery and therapy. Nat Rev Drug Discov (2016) 0.88
Engineering of CRISPR/Cas9-mediated potyvirus resistance in transgene-free Arabidopsis plants. Mol Plant Pathol (2016) 0.87
Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM. Proc Natl Acad Sci U S A (2016) 0.87
Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing. Mol Ther (2016) 0.84
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9. Nat Commun (2016) 0.81
DNA-free genome editing methods for targeted crop improvement. Plant Cell Rep (2016) 0.80
The Rise of CRISPR/Cas for Genome Editing in Stem Cells. Stem Cells Int (2016) 0.78
Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells. Stem Cell Reports (2016) 0.78
Breaking-Cas-interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes. Nucleic Acids Res (2016) 0.77
A quick guide to CRISPR sgRNA design tools. GM Crops Food (2015) 0.77
Mapping the genomic landscape of CRISPR-Cas9 cleavage. Nat Methods (2017) 0.76
CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis. Sci Rep (2016) 0.76
CRISPR/Cas9 in zebrafish: an efficient combination for human genetic diseases modeling. Hum Genet (2016) 0.75
Stabilization of Foxp3 expression by CRISPR-dCas9-based epigenome editing in mouse primary T cells. Epigenetics Chromatin (2017) 0.75
CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment. Sci Rep (2017) 0.75
Learning Parsimonious Classification Rules from Gene Expression Data Using Bayesian Networks with Local Structure. Data (Basel) (2017) 0.75
Sequential organogenesis sets two parallel sensory lines in medaka. Development (2017) 0.75
Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses. Nat Commun (2017) 0.75
Identification of high-efficiency 3'GG gRNA motifs in indexed FASTA files with ngg2. PeerJ Comput Sci (2015) 0.75
Correction: CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool. PLoS One (2017) 0.75
Clustered Regularly Interspaced Short Palindromic Repeats: Challenges in Treating Retinal Disease. Asia Pac J Ophthalmol (Phila) (2016) 0.75
Antisense Oligonucleotide Mediated Splice Correction of a Deep Intronic Mutation in OPA1. Mol Ther Nucleic Acids (2016) 0.75
Dynamics of in vivo ASC speck formation. J Cell Biol (2017) 0.75
CRISPRpred: A flexible and efficient tool for sgRNAs on-target activity prediction in CRISPR/Cas9 systems. PLoS One (2017) 0.75
An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett (2017) 0.75
CRISPR/Cas9: Transcending the Reality of Genome Editing. Mol Ther Nucleic Acids (2017) 0.75
CRISPR/Cas9-mediated knockout of c-REL in HeLa cells results in profound defects of the cell cycle. PLoS One (2017) 0.75
Cas9-mediated excision of Nematostella brachyury disrupts endoderm development, pharynx formation, and oral-aboral patterning. Development (2017) 0.75
Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9. Mol Ther (2017) 0.75
Mitochondrial complex I deficiency leads to the retardation of early embryonic development in Ndufs4 knockout mice. PeerJ (2017) 0.75
The non-coding RNA landscape of human hematopoiesis and leukemia. Nat Commun (2017) 0.75
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol (2009) 235.12
The human genome browser at UCSC. Genome Res (2002) 168.23
Fiji: an open-source platform for biological-image analysis. Nat Methods (2012) 56.92
Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
A TALE nuclease architecture for efficient genome editing. Nat Biotechnol (2010) 26.47
Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol (2013) 21.32
DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013) 19.99
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
RNA-guided genetic silencing systems in bacteria and archaea. Nature (2012) 17.12
Altering the genome by homologous recombination. Science (1989) 12.73
Ensembl 2014. Nucleic Acids Res (2013) 12.62
CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. Annu Rev Genet (2011) 9.88
Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics (2013) 8.56
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol (2013) 8.40
CRISPR-based adaptive immune systems. Curr Opin Microbiol (2011) 8.07
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature (2014) 5.70
Heritable and precise zebrafish genome editing using a CRISPR-Cas system. PLoS One (2013) 2.74
E-CRISP: fast CRISPR target site identification. Nat Methods (2014) 2.73
Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res (2013) 2.60
CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res (2014) 2.36
The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation. Plant Biotechnol J (2014) 1.93
Individual cell migration serves as the driving force for optic vesicle evagination. Science (2006) 1.58
Accelerated homologous recombination and subsequent genome modification in Drosophila. Development (2013) 1.55
Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Genetics (2015) 1.52
Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination. Nucleic Acids Res (2013) 1.49
Medaka spalt acts as a target gene of hedgehog signaling. Development (1997) 1.34
Medaka eyeless is the key factor linking retinal determination and eye growth. Development (2001) 1.32
Targeted mutagenesis using CRISPR/Cas system in medaka. Biol Open (2014) 1.18
Multiple genome modifications by the CRISPR/Cas9 system in zebrafish. Genes Cells (2014) 1.17
Efficient homologous recombination-mediated genome engineering in zebrafish using TALE nucleases. Development (2014) 1.16
Fate restriction and multipotency in retinal stem cells. Cell Stem Cell (2011) 1.09
Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. PLoS One (2013) 0.92
Colored medaka and zebrafish: transgenics with ubiquitous and strong transgene expression driven by the medaka β-actin promoter. Dev Growth Differ (2012) 0.82
A lens-specific co-injection marker for medaka transgenesis. Biotechniques (2010) 0.81