Published in Nature on January 29, 2014
Development and applications of CRISPR-Cas9 for genome engineering. Cell (2014) 13.50
Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell (2014) 5.26
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science (2014) 5.13
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat Biotechnol (2014) 5.07
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol (2014) 4.41
Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature (2015) 3.77
Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease. Nature (2014) 3.72
High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature (2016) 3.54
Unravelling the structural and mechanistic basis of CRISPR-Cas systems. Nat Rev Microbiol (2014) 3.48
Rationally engineered Cas9 nucleases with improved specificity. Science (2015) 3.14
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A (2014) 2.97
CRISPR-Cas systems: Prokaryotes upgrade to adaptive immunity. Mol Cell (2014) 2.87
Concerning RNA-guided gene drives for the alteration of wild populations. Elife (2014) 2.69
Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods (2015) 2.42
High-throughput functional genomics using CRISPR-Cas9. Nat Rev Genet (2015) 2.35
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Nat Biotechnol (2016) 2.34
Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature (2014) 2.33
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol (2016) 2.05
CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool. PLoS One (2015) 1.92
A mouse geneticist's practical guide to CRISPR applications. Genetics (2014) 1.81
Principles of genetic circuit design. Nat Methods (2014) 1.79
Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Cell (2016) 1.75
DNA sequence alignment by microhomology sampling during homologous recombination. Cell (2015) 1.75
Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes. Proc Natl Acad Sci U S A (2014) 1.75
CRISPR-Cas immunity in prokaryotes. Nature (2015) 1.75
Cas9 specifies functional viral targets during CRISPR-Cas adaptation. Nature (2015) 1.70
The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA. Nature (2016) 1.64
A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans. Genetics (2014) 1.60
Sequence determinants of improved CRISPR sgRNA design. Genome Res (2015) 1.56
Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Genetics (2015) 1.52
Regulation of transcription by long noncoding RNAs. Annu Rev Genet (2014) 1.43
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition. Nat Biotechnol (2015) 1.41
Genome-editing Technologies for Gene and Cell Therapy. Mol Ther (2016) 1.41
Crystal Structure of Staphylococcus aureus Cas9. Cell (2015) 1.40
Making designer mutants in model organisms. Development (2014) 1.38
Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage. PLoS One (2014) 1.38
A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol (2014) 1.37
A CRISPR/Cas9 vector system for tissue-specific gene disruption in zebrafish. Dev Cell (2015) 1.36
Conformational control of DNA target cleavage by CRISPR-Cas9. Nature (2015) 1.35
Genome-wide identification of CRISPR/Cas9 off-targets in human genome. Cell Res (2014) 1.32
Selection of chromosomal DNA libraries using a multiplex CRISPR system. Elife (2014) 1.31
Cas9 function and host genome sampling in Type II-A CRISPR-Cas adaptation. Genes Dev (2015) 1.27
Editing the epigenome: technologies for programmable transcription and epigenetic modulation. Nat Methods (2016) 1.24
Target specificity of the CRISPR-Cas9 system. Quant Biol (2014) 1.23
Off-target Effects in CRISPR/Cas9-mediated Genome Engineering. Mol Ther Nucleic Acids (2015) 1.23
Nucleosomes impede Cas9 access to DNA in vivo and in vitro. Elife (2016) 1.20
A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture. Nucleic Acids Res (2015) 1.20
Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9. Mol Cell (2016) 1.19
Cas9-Guide RNA Directed Genome Editing in Soybean. Plant Physiol (2015) 1.18
Mechanism of foreign DNA recognition by a CRISPR RNA-guided surveillance complex from Pseudomonas aeruginosa. Nucleic Acids Res (2015) 1.17
Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci. Nucleic Acids Res (2016) 1.14
Two distinct DNA binding modes guide dual roles of a CRISPR-Cas protein complex. Mol Cell (2015) 1.13
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol (2015) 1.12
Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans. Sci Rep (2014) 1.12
RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection. Proc Natl Acad Sci U S A (2014) 1.10
Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference. Nucleic Acids Res (2015) 1.10
CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering. Genetics (2016) 1.10
CRISPR interference and priming varies with individual spacer sequences. Nucleic Acids Res (2015) 1.10
A Dynamic Search Process Underlies MicroRNA Targeting. Cell (2015) 1.09
Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR. Mol Cell (2015) 1.09
Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System. Cell (2015) 1.09
CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc Natl Acad Sci U S A (2015) 1.08
Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9. J Mol Cell Biol (2015) 1.07
The structural biology of CRISPR-Cas systems. Curr Opin Struct Biol (2015) 1.07
CRISPR immunity drives rapid phage genome evolution in Streptococcus thermophilus. MBio (2015) 1.06
Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Nature (2016) 1.05
Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus. Dev Genes Evol (2014) 1.05
Poised epigenetic states and acquired drug resistance in cancer. Nat Rev Cancer (2014) 1.05
One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system. Cell Res (2014) 1.03
Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases. Nat Rev Genet (2016) 1.03
An efficient genotyping method for genome-modified animals and human cells generated with CRISPR/Cas9 system. Sci Rep (2014) 1.02
Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Nat Commun (2015) 1.00
Depletion of Abundant Sequences by Hybridization (DASH): using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applications. Genome Biol (2016) 1.00
Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles. Planta (2014) 1.00
Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3. Proc Natl Acad Sci U S A (2014) 1.00
A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. Dev Cell (2014) 0.99
Synthetic epigenetics-towards intelligent control of epigenetic states and cell identity. Clin Epigenetics (2015) 0.99
Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery. Nat Methods (2015) 0.96
DNA repair mechanisms and their biological roles in the malaria parasite Plasmodium falciparum. Microbiol Mol Biol Rev (2014) 0.96
Identification of genes that promote or inhibit olfactory memory formation in Drosophila. Genetics (2015) 0.95
Nucleosome breathing and remodeling constrain CRISPR-Cas9 function. Elife (2016) 0.94
Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements. Genome Biol (2015) 0.93
Determining the specificities of TALENs, Cas9, and other genome-editing enzymes. Methods Enzymol (2014) 0.93
Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression. Methods (2014) 0.93
Cut site selection by the two nuclease domains of the Cas9 RNA-guided endonuclease. J Biol Chem (2014) 0.93
Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos. Sci Rep (2015) 0.93
Landscape of target:guide homology effects on Cas9-mediated cleavage. Nucleic Acids Res (2014) 0.93
Identification of proteins associated with an IFNγ-responsive promoter by a retroviral expression system for enChIP using CRISPR. PLoS One (2014) 0.92
Genome-wide specificity of DNA binding, gene regulation, and chromatin remodeling by TALE- and CRISPR/Cas9-based transcriptional activators. Genome Res (2015) 0.92
How specific is CRISPR/Cas9 really? Curr Opin Chem Biol (2015) 0.91
Heritable CRISPR/Cas9-mediated genome editing in the yellow fever mosquito, Aedes aegypti. PLoS One (2015) 0.91
Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity. Mol Cell (2016) 0.91
Enabling functional genomics with genome engineering. Genome Res (2015) 0.90
CRISPR-Cas systems: new players in gene regulation and bacterial physiology. Front Cell Infect Microbiol (2014) 0.89
Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing. PLoS One (2015) 0.89
Haloarcula hispanica CRISPR authenticates PAM of a target sequence to prime discriminative adaptation. Nucleic Acids Res (2014) 0.89
Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems. Mol Cell (2016) 0.89
Mechanism of CRISPR-RNA guided recognition of DNA targets in Escherichia coli. Nucleic Acids Res (2015) 0.88
Bacterial CRISPR: accomplishments and prospects. Curr Opin Microbiol (2015) 0.88
DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods (2015) 0.88
Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
CRISPR provides acquired resistance against viruses in prokaryotes. Science (2007) 29.74
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell (2013) 24.00
Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol (2013) 21.32
DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013) 19.99
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol (2013) 18.01
Small CRISPR RNAs guide antiviral defense in prokaryotes. Science (2008) 17.79
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature (2011) 17.38
RNA-guided genetic silencing systems in bacteria and archaea. Nature (2012) 17.12
Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A (2012) 16.48
RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol (2013) 15.51
RNA-programmed genome editing in human cells. Elife (2013) 14.82
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature (2010) 14.21
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell (2013) 13.43
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol (2013) 11.35
Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology (2009) 10.67
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell (2013) 9.55
Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics (2013) 8.56
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol (2013) 8.40
Facilitated target location in biological systems. J Biol Chem (1989) 7.18
Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence. Proc Natl Acad Sci U S A (2011) 6.89
Direct observation of base-pair stepping by RNA polymerase. Nature (2005) 6.29
Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods (2013) 5.93
Self versus non-self discrimination during CRISPR RNA-directed immunity. Nature (2010) 5.89
RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods (2013) 4.85
Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system. Cell Rep (2013) 4.72
CRISPR RNA-guided activation of endogenous human genes. Nat Methods (2013) 4.69
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods (2013) 4.61
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions. Proc Natl Acad Sci U S A (2011) 4.58
Origins of specificity in protein-DNA recognition. Annu Rev Biochem (2010) 4.43
Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol (2013) 4.19
Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol (2013) 3.84
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A (2013) 3.53
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res (2013) 3.51
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol (2013) 3.20
On the specificity of DNA-protein interactions. Proc Natl Acad Sci U S A (1986) 3.11
Mechanism of substrate selection by a highly specific CRISPR endoribonuclease. RNA (2012) 2.78
Mechanism of foreign DNA selection in a bacterial adaptive immune system. Mol Cell (2012) 2.77
RNA-guided genome editing in plants using a CRISPR-Cas system. Mol Plant (2013) 2.25
Visualizing one-dimensional diffusion of eukaryotic DNA repair factors along a chromatin lattice. Nat Struct Mol Biol (2010) 2.17
Single-molecule imaging reveals target-search mechanisms during DNA mismatch repair. Proc Natl Acad Sci U S A (2012) 1.72
Single-molecule imaging reveals mechanisms of protein disruption by a DNA translocase. Nature (2010) 1.59
Single-molecule imaging of DNA curtains reveals intrinsic energy landscapes for nucleosome deposition. Nat Struct Mol Biol (2009) 1.58
DNA curtains and nanoscale curtain rods: high-throughput tools for single molecule imaging. Langmuir (2008) 1.39
Nanofabricated racks of aligned and anchored DNA substrates for single-molecule imaging. Langmuir (2010) 1.23
The promoter-search mechanism of Escherichia coli RNA polymerase is dominated by three-dimensional diffusion. Nat Struct Mol Biol (2012) 1.19