Fetal lamb 3 beta, 20 alpha-hydroxysteroid oxidoreductase: dual activity at the same active site examined by affinity labeling with 16 alpha-(bromo[2'-14C]acetoxy)progesterone.

PubWeight™: 0.79‹?›

🔗 View Article (PMID 2605227)

Published in Biochemistry on October 31, 1989

Authors

Q X Chen1, L O Rosik, C D Nancarrow, F Sweet

Author Affiliations

1: Division of Reproductive Biology, Washington University School of Medicine, St. Louis, Missouri 63110.

Articles by these authors

The chemistry of some 5-(2-hydroxyalkyl)uracil derivatives and a synthesis of 5-vinyluracil. J Org Chem (1973) 1.22

Synthesis of 5-carboxymethyluridine. A nucleoside from transfer ribonucleic acid. Biochemistry (1970) 1.10

The chemistry of the base-catalyzed condensation of some 3-alkoxy-and 3-alkoxy-2-dialkoxymethyl esters with ureas. Synthesis of 5-substituted uracils. J Org Chem (1973) 1.05

The early pregnancy factor of sheep and cattle. J Reprod Fertil Suppl (1981) 0.92

Ozone selectively inhibits growth of human cancer cells. Science (1980) 0.90

Affinity labeling of hormone-specific proteins. Endocr Rev (1987) 0.89

Detection of early pregnancy in sheep by the rosette inhibition test. J Reprod Fertil (1979) 0.88

Regulation of expression of a sheep metallothionein 1a-sheep growth hormone fusion gene in transgenic mice. Mol Cell Biol (1989) 0.88

Synthesis and use of affinity labeling steroids for analysis of macromolecular steroid-binding sites. Methods Enzymol (1975) 0.85

Bifunctional enzyme activity at the same active site: study of 3 alpha and 20 beta activity by affinity alkylation of 3 alpha, 20 beta-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroids. Biochemistry (1980) 0.85

Affinity labeling of steroid binding sites. Study of the active site of 20beta-hydroxysteroid dehydrogenase with 2alpha-bromoacetoxyprogesterone and 11alpha-bromacetoxyprogesterone. J Biol Chem (1975) 0.85

Production of transgenic merino sheep by microinjection of ovine metallothionein-ovine growth hormone fusion genes. Reprod Fertil Dev (1989) 0.84

Affinity labeling of steroid binding sites. Synthesis of 16 -bromoacetoxyprogesterone and its use for affinity labeling of 20 -hydroxysteroid dehydrogenase. J Biol Chem (1972) 0.84

Affinity labeling of steroid binding sites. Study of the active site of 20-beta-hydroxysteroid dehydrogenase with 6-beta- and 11 alpha-bromoacetoxyprogesterone. J Biol Chem (1973) 0.84

Linear bone growth of oMT1a-oGH transgenic male mice. Am J Physiol (1992) 0.83

Development of ovine embryos in synthetic oviductal fluid containing amino acids at oviductal fluid concentrations. Biol Reprod (1996) 0.83

Novel synthesis of cholesterol analogs: condensation of pregnenolone with dihydropyran or dihydrofuran. Steroids (1991) 0.82

The commercial and agricultural applications of animal transgenesis. Mol Biotechnol (1995) 0.81

Dual activity at an enzyme active site: 3 beta,20 alpha-hydroxysteroid oxidoreductase from fetal blood. Biochemistry (1982) 0.81

Growth of Graafian follicles in cows following luteolysis induced by the prostaglandin F2 alpha analogue, cloprostenol. Aust J Biol Sci (1980) 0.81

Complete 1H and 13C NMR spectra of pregnenolone. Steroids (1995) 0.80

Daunorubicin conjugated to a monoclonal anti-CA125 antibody selectively kills human ovarian cancer cells. Gynecol Oncol (1989) 0.80

Synthesis of an affinity chromatography column designed for recovery of labile proteins. Biochem Biophys Res Commun (1975) 0.80

Affinity alkylation of human placental 3 beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5----4-ene-isomerase by 2 alpha-bromoacetoxyprogesterone: evidence for separate dehydrogenase and isomerase sites on one protein. J Steroid Biochem (1990) 0.79

Affinity labeling of steroid binding sites. Synthesis of 21-bromoacetylaminoprogesterone and study of 20beta-hydroxysteroid dehydrogenase. J Biol Chem (1978) 0.79

Polyploid cells in blastocysts and early fetuses from Australian Merino sheep. J Reprod Fertil (1986) 0.78

Synthesis of a disulfide affinity adsorbent for purification of estrogen receptor. Steroids (1977) 0.78

Interaction of the radiolabelled high-affinity anti-oestrogen [3H]H1285 with the cytoplasmic oestrogen receptor. Biochem J (1984) 0.78

Isolation of 3 beta,20 alpha-hydroxysteroid oxidoreductase from sheep fetal blood. Steroids (1987) 0.78

Introduction and expression of the bacterial glyoxylate cycle genes in transgenic mice. Transgenic Res (1996) 0.77

The potential of transgenic animals for improved agricultural productivity. Rev Sci Tech (1990) 0.77

The genetic engineering of production traits in domestic animals. Experientia (1991) 0.77

Ovarian function in suckling and non-suckling beef cows post partum. J Reprod Fertil (1978) 0.77

Purification of 20 alpha-hydroxysteroid oxidoreductase from bovine fetal erythrocytes. Steroids (1981) 0.77

Ultrastructural changes in the oviductal epithelium of Merino ewes during the estrous cycle. Am J Anat (1984) 0.77

Influence of ovine oviducal amino acid concentrations and an ovine oestrus-associated glycoprotein on development and viability of bovine embryos. Mol Reprod Dev (1997) 0.77

Human placental estradiol 17 beta-dehydrogenase: evidence for inverted substrate orientation ("wrong-way" binding) at the active site. Biochemistry (1988) 0.77

The effect of 2-deoxy-D-glucose on ovarian function of cattle. Aust J Biol Sci (1978) 0.77

Identification of an oestrus-associated glycoprotein in oviducal fluid of the sheep. J Reprod Fertil (1984) 0.77

Affinity alkylation of the active site of C21 steroid side-chain cleavage cytochrome P-450 from neonatal porcine testis: a unique cysteine residue alkylated by 17-(bromoacetoxy)progesterone. Biochemistry (1987) 0.76

Hormonal changes around oestrus in the cow. J Reprod Fertil (1973) 0.76

Relationship between glucose and 20 alpha-hydroxysteroid dehydrogenase in fetal sheep erythrocytes. J Endocrinol (1984) 0.76

Detection of induced death of embryos in sheep by the rosette inhibition test. J Reprod Fertil (1979) 0.76

Evaluation of a rosette inhibition test for pregnancy diagnosis in pigs. J Reprod Immunol (1985) 0.75

Zygote viability in gene transfer experiments. Biol Reprod (1987) 0.75

The effect of thyrotrophin releasing hormone on pituitary and thyroid function in pre- and post-natal lambs. Acta Endocrinol (Copenh) (1979) 0.75

Effect of anti-oestradiol-17B antibodies on the reproductive response of ewes superovulated with PMSG. Aust J Biol Sci (1988) 0.75

An in vitro system for assessing lung cell response to ozone. J Air Pollut Control Assoc (1981) 0.75

Effect of anaesthesia on ovarian follicular development and ovulation in the sheep subsequent to prostaglandin-induced luteolysis. J Endocrinol (1978) 0.75

Fertilization and early embryonic development in androstenedione-immunized Merino ewes. J Reprod Fertil (1986) 0.75

Preventing hair loss in mice. Nature (1994) 0.75

Steroid-albumin conjugate interaction with steroid-binding proteins. Endocrinology (1980) 0.75

Ovarian response to PMSG treatment in ewes immunized against oestradiol-17 beta. Aust J Biol Sci (1985) 0.75

Synthesis and antiestrogenic activity of 2,4-bis(bromomethyl)-estrone methyl ether: a bifunctional alkylating steroid. Steroids (1974) 0.75

Interceptive activity of 16alpha-bromoacetoxyprogesterone. Biol Reprod (1974) 0.75

Electrophilic analogues of daunorubicin and doxorubicin. Bioconjug Chem (1991) 0.75

5'-bromoacetamido-5'-deoxyadenosine. A novel reagent for labeling adenine nucleotide sites in proteins. J Biol Chem (1983) 0.75

The metabolism of progesterone and the conservation of gestagens in the sheep foeto-placental unit. J Endocrinol (1972) 0.75

Affinity labeling of 3 alpha, 20 beta-hydroxysteroid dehydrogenase with a nucleoside analog. Steroids (1980) 0.75

Decline with age in the rate of reduction of progesterone to 20 alpha-hydroxypregn-4-en-3-one in the blood of perinatal ruminants. Aust J Biol Sci (1983) 0.75

Partial purification and properties of an NADPH 20 -hydroxysteroid oxidoreductase from foetal sheep blood. J Endocrinol (1972) 0.75

Cytogenetic analysis of 34 early stage bovine embryos from superovulated Hereford donors. Can J Genet Cytol (1985) 0.75

Proceedings: intramuscular administration of a prostaglandin analogue during pregnancy in the goat. J Reprod Fertil (1975) 0.75

Ovarian response to PMSG and GnRH in ewes immunised against oestradiol-17 beta. Vet Rec (1987) 0.75

Proceedings: superovulation and surgical collection of ova from cows on each of two successive cycles. J Reprod Fertil (1975) 0.75

Proceedings: use of oestradiol benzoate to improve synchronization of oestrus in cattle. J Reprod Fertil (1975) 0.75

The commercial and agricultural applications of animal transgenesis. Methods Mol Biol (1993) 0.75

Termination of early pregnancy in ewes by use of a prostaglandin analogue and subsequent fertility. Aust Vet J (1981) 0.75

Progesterone metabolism in ovine blood: the formation of 3 alpha-hydroxy-pregn-4-en-20-one and other substances. Steroids (1970) 0.75

Occurrence of haploid and haploid/diploid mosaic embryos in untreated and androstenedione-immune Australian Merino sheep. J Reprod Fertil (1985) 0.75

Polyploid abnormalities in day 3 and day 5 merino sheep embryos. Aust J Biol Sci (1988) 0.75

Nicotinamide adenine dinucleotide binding and promotion of enzyme activity: model based on affinity labeling of 3 alpha, 20 beta-hydroxysteroid dehydrogenase with a nucleoside. Biochemistry (1981) 0.75

Human ovarian cancers specifically bind daunorubicin-OC-125 conjugate: an immunofluorescence study. Gynecol Oncol (1990) 0.75

Modulation of functional capacity of small ovarian follicles in the post-partum cow by prolactin. Aust J Biol Sci (1981) 0.75

Effect of embryos on luteolysis and termination of early pregnancy in sheep with cloprostenol. Biol Reprod (1982) 0.75

Secretion of granules by the luteal cells of the sheep and the goat during the estrous cycle and pregnancy. Anat Rec (1977) 0.75

Inhibition of progesterone secretion and decrease of ovarian blood flow by isoxazole in pregnant rats. Acta Physiol Hung (1987) 0.75

Proceedings: Hormonal changes occurring in cattle following the administration of prostaglandin F 2alpha. J Reprod Fertil (1974) 0.75

Synthesis of 21-amino-5-pregnene-3,20-dione bisethylene ketal. Steroids (1977) 0.75

High-field NMR studies of 3beta-tetrahydropyranyloxy steroids. Steroids (2000) 0.75

Synthesis and cyclization of 21-hydroxyethylthioprogesterone derivatives. J Org Chem (1976) 0.75

Chemistry of trifluoroacetic anhydride-haloacetic acid reactions with medroxyprogesterone. J Org Chem (1977) 0.75

Progesterone metabolism in fetal blood. Steroids (1968) 0.75

Synthesis and use of affinity-labeling steroids for interceptive purposes. Am J Obstet Gynecol (1975) 0.75

Cloning and sequencing of a cDNA encoding an ovine oestrus-associated oviducal protein. Reprod Fertil Dev (1996) 0.75

Substrate characteristics of progesterone-albumin conjugates with 20beta-hydroxysteroid dehydrogenase. Biochem Biophys Res Commun (1978) 0.75

Bifunctional enzyme activity at the same active site: competitive inhibition kinetics with 3 alpha/20 beta-hydroxysteroid dehydrogenase. Steroids (1980) 0.75

Reproductive wastage in the androstenedione-immune ewe. J Reprod Fertil (1993) 0.75

Proceedings: The effect of the mode of administration of prostaglandin F 2alpha on the synchronization of oestrus in cattle. J Reprod Fertil (1974) 0.75

Synthesis of 21-acryloxyprogesterone, 21-bromoacetoxyprogesterone and 11 -bromacetoxyprogesterone for affinity labeling. Biochim Biophys Acta (1972) 0.75

Progesterone metabolism in foetal sheep gonadal tissue in vitro. J Endocrinol (1972) 0.75

Reactivation studies of an affinity labeled steroid oxido-reductase. I. Inactivation of 20 beta-hydroxysteroid dehydrogenase with 6 beta-bromoacetoxyprogesterone vs 6 beta-bromoprogesterone. Steroids (1976) 0.75

Oestrogen and seasonal effects on the production of an oestrus-associated glycoprotein in oviducal fluid of sheep. J Reprod Fertil (1986) 0.75

Synthesis of medroxyprogesterone bromoacetate for affinity labeling. J Med Chem (1977) 0.75

Boron estrogens: synthesis, biochemical and biological testing of estrone and estradiol-17 beta 3-carboranylmethyl ethers. Steroids (1981) 0.75