Published in Wiley Interdiscip Rev Syst Biol Med on September 22, 2015
Potential Biotechnological Strategies for the Cleanup of Heavy Metals and Metalloids. Front Plant Sci (2016) 0.77
Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell (2013) 24.00
Breaking the code of DNA binding specificity of TAL-type III effectors. Science (2009) 22.33
A simple cipher governs DNA recognition by TAL effectors. Science (2009) 19.99
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res (2011) 16.53
RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol (2013) 15.51
A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet (1998) 15.27
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli. J Bacteriol (1998) 8.59
Bacterial gene transfer by natural genetic transformation in the environment. Microbiol Rev (1994) 8.52
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res (2013) 7.77
In vivo genome editing using a high-efficiency TALEN system. Nature (2012) 7.11
Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res (1999) 6.85
Programming cells by multiplex genome engineering and accelerated evolution. Nature (2009) 6.64
Targeted chromosomal deletions in human cells using zinc finger nucleases. Genome Res (2009) 6.24
Functional genomic hypothesis generation and experimentation by a robot scientist. Nature (2004) 5.41
The automation of science. Science (2009) 5.34
Efficient TALEN-mediated gene knockout in livestock. Proc Natl Acad Sci U S A (2012) 4.99
Genome engineering. Nat Biotechnol (2009) 4.60
Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol (2013) 4.58
Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A (2013) 4.57
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol (2014) 4.41
Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol (2013) 4.19
Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol (2013) 3.84
Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat Biotechnol (2013) 3.65
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat Biotechnol (2015) 3.64
High-efficiency TALEN-based gene editing produces disease-resistant rice. Nat Biotechnol (2012) 3.61
Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations. Science (2015) 3.56
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement. Science (2011) 3.49
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat Biotechnol (2014) 3.22
Precision genome engineering with programmable DNA-nicking enzymes. Genome Res (2012) 3.05
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A (2014) 2.97
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol (2015) 2.77
Chromosomal translocations induced at specified loci in human stem cells. Proc Natl Acad Sci U S A (2009) 2.73
E-CRISP: fast CRISPR target site identification. Nat Methods (2014) 2.73
Recoded organisms engineered to depend on synthetic amino acids. Nature (2015) 2.71
Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy. Nat Commun (2015) 2.56
Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs). Proc Natl Acad Sci U S A (2012) 2.53
High-throughput functional genomics using CRISPR-Cas9. Nat Rev Genet (2015) 2.35
Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases. Biotechnol Bioeng (2010) 2.23
Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells. Mol Cell (2014) 2.02
Optimized TAL effector nucleases (TALENs) for use in treatment of sickle cell disease. Mol Biosyst (2012) 2.01
Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion. Nucleic Acids Res (2010) 1.92
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep (2014) 1.83
Highly efficient and specific genome editing in silkworm using custom TALENs. PLoS One (2012) 1.80
TALEN-engineered AR gene rearrangements reveal endocrine uncoupling of androgen receptor in prostate cancer. Proc Natl Acad Sci U S A (2013) 1.77
Genome-scale promoter engineering by coselection MAGE. Nat Methods (2012) 1.69
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A (2015) 1.68
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res (2014) 1.59
Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice. Nucleic Acids Res (2014) 1.58
Targeted chromosomal deletions and inversions in zebrafish. Genome Res (2013) 1.57
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis. Development (2014) 1.53
Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Genetics (2015) 1.52
CRISPR multitargeter: a web tool to find common and unique CRISPR single guide RNA targets in a set of similar sequences. PLoS One (2015) 1.51
Multiplexed genome engineering and genotyping methods applications for synthetic biology and metabolic engineering. Methods Enzymol (2011) 1.46
Cancer translocations in human cells induced by zinc finger and TALE nucleases. Genome Res (2013) 1.44
Tapping natural reservoirs of homing endonucleases for targeted gene modification. Proc Natl Acad Sci U S A (2011) 1.39
A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol (2014) 1.37
Selection of chromosomal DNA libraries using a multiplex CRISPR system. Elife (2014) 1.31
Transcription activator-like effector nucleases (TALENs): a highly efficient and versatile tool for genome editing. Biotechnol Bioeng (2013) 1.28
Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion. Nucleic Acids Res (2011) 1.28
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae. FEMS Yeast Res (2015) 1.25
Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system. Appl Environ Microbiol (2015) 1.21
Multiple genome modifications by the CRISPR/Cas9 system in zebrafish. Genes Cells (2014) 1.17
Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae. Metab Eng (2015) 1.17
High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system. ACS Synth Biol (2014) 1.17
Homology-integrated CRISPR-Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae. ACS Synth Biol (2014) 1.16
Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems. Sci Rep (2014) 1.10
Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases. Dev Growth Differ (2013) 1.03
Multigene knockout utilizing off-target mutations of the CRISPR/Cas9 system in rice. Plant Cell Physiol (2014) 1.02
Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases. PLoS One (2012) 1.02
CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori. Sci Rep (2014) 1.00
Yeast oligo-mediated genome engineering (YOGE). ACS Synth Biol (2013) 1.00
Multiplex genome editing by natural transformation. Proc Natl Acad Sci U S A (2014) 0.99
Synthetic epigenetics-towards intelligent control of epigenetic states and cell identity. Clin Epigenetics (2015) 0.99
Recent advances in targeted genome engineering in mammalian systems. Biotechnol J (2012) 0.97
Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Genetics (2015) 0.96
FairyTALE: a high-throughput TAL effector synthesis platform. ACS Synth Biol (2013) 0.94
Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering. Nucleic Acids Res (2012) 0.94
Inheritable and precise large genomic deletions of non-coding RNA genes in zebrafish using TALENs. PLoS One (2013) 0.93
Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression. Methods (2014) 0.93
One-step generation of phenotype-expressing triple-knockout mice with heritable mutated alleles by the CRISPR/Cas9 system. J Reprod Dev (2014) 0.92
Direct mutagenesis of thousands of genomic targets using microarray-derived oligonucleotides. ACS Synth Biol (2014) 0.91
Creation of fragrant rice by targeted knockout of the OsBADH2 gene using TALEN technology. Plant Biotechnol J (2015) 0.89
CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae. ACS Synth Biol (2015) 0.89
A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish. Methods (2014) 0.89
Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9. Insect Biochem Mol Biol (2014) 0.87
SunnyTALEN: a second-generation TALEN system for human genome editing. Biotechnol Bioeng (2013) 0.87
Multiplex engineering of industrial yeast genomes using CRISPRm. Methods Enzymol (2014) 0.87
MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering. Nucleic Acids Res (2014) 0.87
Synthetic biology: putting synthesis into biology. Wiley Interdiscip Rev Syst Biol Med (2010) 0.87
One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment. Biotechnol J (2015) 0.86
Bacterial Recombineering: Genome Engineering via Phage-Based Homologous Recombination. ACS Synth Biol (2015) 0.86
Multiplex genomic structure variation mediated by TALEN and ssODN. BMC Genomics (2014) 0.84
Building biological foundries for next-generation synthetic biology. Sci China Life Sci (2015) 0.79
Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Nat Protoc (2014) 0.79
High Throughput Screening and Selection Methods for Directed Enzyme Evolution. Ind Eng Chem Res (2014) 0.78
Synthetic biology for therapeutic applications. Mol Pharm (2014) 0.78
Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae. J Biosci Bioeng (2015) 0.78
Directed evolution as a powerful synthetic biology tool. Methods (2012) 0.91