Published in Appl Environ Microbiol on September 30, 2016
Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
Stochastic gene expression in a single cell. Science (2002) 34.54
CRISPR provides acquired resistance against viruses in prokaryotes. Science (2007) 29.74
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell (2013) 24.00
Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods (2009) 22.47
Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol (2011) 17.11
Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A (2012) 16.48
RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol (2013) 15.51
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell (2013) 13.43
Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise. Nature (2006) 12.92
Control of stochasticity in eukaryotic gene expression. Science (2004) 12.85
Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science (2014) 10.43
TAL effectors: customizable proteins for DNA targeting. Science (2011) 9.47
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res (2013) 7.77
CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet (2010) 7.47
The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats. BMC Bioinformatics (2007) 7.34
Programming cells by multiplex genome engineering and accelerated evolution. Nature (2009) 6.64
Carbon catabolite repression in bacteria: many ways to make the most out of nutrients. Nat Rev Microbiol (2008) 6.24
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature (2014) 5.70
CRISPR-based adaptive and heritable immunity in prokaryotes. Trends Biochem Sci (2009) 5.54
The ClosTron: a universal gene knock-out system for the genus Clostridium. J Microbiol Methods (2007) 4.20
In vivo and in vitro transcription of the Clostridium pasteurianum ferredoxin gene. Evidence for "extended" promoter elements in gram-positive organisms. J Biol Chem (1986) 3.94
An updated evolutionary classification of CRISPR-Cas systems. Nat Rev Microbiol (2015) 3.74
The ClosTron: Mutagenesis in Clostridium refined and streamlined. J Microbiol Methods (2009) 3.16
Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila. Genetics (2014) 3.07
Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets. Nat Biotechnol (2005) 2.95
In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol (1993) 2.70
A modular system for Clostridium shuttle plasmids. J Microbiol Methods (2009) 2.49
Genetic compensation induced by deleterious mutations but not gene knockdowns. Nature (2015) 2.40
Use of computer-designed group II introns to disrupt Escherichia coli DExH/D-box protein and DNA helicase genes. J Mol Biol (2004) 2.18
Reporter proteins for in vivo fluorescence without oxygen. Nat Biotechnol (2007) 2.11
Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824. J Bacteriol (2002) 2.07
Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria. Nat Biotechnol (2001) 2.06
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res (2013) 1.83
Global transcription machinery engineering: a new approach for improving cellular phenotype. Metab Eng (2007) 1.81
Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824. Microbiology (1996) 1.78
New protein kinase and protein phosphatase families mediate signal transduction in bacterial catabolite repression. Proc Natl Acad Sci U S A (1998) 1.75
Engineering solventogenic clostridia. Curr Opin Biotechnol (2008) 1.72
Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production. Appl Environ Microbiol (2012) 1.70
Phenotypic alteration of eukaryotic cells using randomized libraries of artificial transcription factors. Nat Biotechnol (2003) 1.65
Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications. Curr Opin Biotechnol (2011) 1.60
Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum. Appl Environ Microbiol (1999) 1.50
Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker. Nucleic Acids Res (2012) 1.47
Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia. Biotechnol Biofuels (2014) 1.47
Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Cell Res (2007) 1.45
Design of antisense RNA constructs for downregulation of the acetone formation pathway of Clostridium acetobutylicum. J Bacteriol (2003) 1.43
Purification and characterization of glpX-encoded fructose 1, 6-bisphosphatase, a new enzyme of the glycerol 3-phosphate regulon of Escherichia coli. J Bacteriol (2000) 1.36
Combinatorial engineering of microbes for optimizing cellular phenotype. Curr Opin Chem Biol (2008) 1.31
Hitting bacteria at the heart of the central dogma: sequence-specific inhibition. Microb Cell Fact (2007) 1.22
Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration. Appl Environ Microbiol (2012) 1.20
Successful transient expression of Cas9 and single guide RNA genes in Chlamydomonas reinhardtii. Eukaryot Cell (2014) 1.19
Transcriptional organization of the Clostridium acetobutylicum genome. Nucleic Acids Res (2004) 1.16
Engineering clostridium strain to accept unmethylated DNA. PLoS One (2010) 1.11
Expression of Clostridium acetobutylicum ATCC 824 genes in Escherichia coli for acetone production and acetate detoxification. Appl Environ Microbiol (1998) 1.05
Development and application of a method for counterselectable in-frame deletion in Clostridium perfringens. Appl Environ Microbiol (2010) 1.05
Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase. Appl Environ Microbiol (2015) 1.01
Gene silencing by CRISPR interference in mycobacteria. Nat Commun (2015) 1.00
Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum. Biotechnol Biofuels (2013) 0.95
Markerless chromosomal gene deletion in Clostridium beijerinckii using CRISPR/Cas9 system. J Biotechnol (2015) 0.94
Disruption of a toxin gene by introduction of a foreign gene into the chromosome of Clostridium perfringens using targetron-induced mutagenesis. Plasmid (2007) 0.93
Analysis of the elements of catabolite repression in Clostridium acetobutylicum ATCC 824. J Mol Microbiol Biotechnol (2003) 0.91
Engineering butanol-tolerance in escherichia coli with artificial transcription factor libraries. Biotechnol Bioeng (2010) 0.91
Technical guide for genetic advancement of underdeveloped and intractable Clostridium. Biotechnol Adv (2014) 0.90
HPr kinase/phosphorylase, the sensor enzyme of catabolite repression in Gram-positive bacteria: structural aspects of the enzyme and the complex with its protein substrate. J Bacteriol (2003) 0.87
Restriction for gene insertion within the Lactococcus lactis Ll.LtrB group II intron. RNA (2006) 0.87
Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme. Biotechnol Biofuels (2014) 0.84
Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis. Appl Environ Microbiol (2016) 0.81
Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes. Curr Opin Biotechnol (2014) 0.81
Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium. Sci Rep (2016) 0.80
A functional recT gene for recombineering of Clostridium. J Biotechnol (2013) 0.77
Whole genome sequencing reveals a novel CRISPR system in industrial Clostridium acetobutylicum. J Ind Microbiol Biotechnol (2014) 0.77
Elimination of carbon catabolite repression in Clostridium acetobutylicum--a journey toward simultaneous use of xylose and glucose. Appl Microbiol Biotechnol (2015) 0.76
Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli. Appl Environ Microbiol (2015) 1.07
Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis. Appl Environ Microbiol (2016) 0.81
Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium. Sci Rep (2016) 0.80
Improved Draft Genome Sequence of Clostridium pasteurianum Strain ATCC 6013 (DSM 525) Using a Hybrid Next-Generation Sequencing Approach. Genome Announc (2014) 0.79
Disruption of the Reductive 1,3-Propanediol Pathway Triggers Production of 1,2-Propanediol for Sustained Glycerol Fermentation by Clostridium pasteurianum. Appl Environ Microbiol (2016) 0.78
Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum. Sci Rep (2016) 0.77
Engineering of cell membrane to enhance heterologous production of hyaluronic acid in Bacillus subtilis. Biotechnol Bioeng (2017) 0.75