Decoupling the effects of stiffness and fiber density on cellular behaviors via an interpenetrating network of gelatin-methacrylate and collagen.

PubWeight™: 0.75‹?›

🔗 View Article (PMID 28683337)

Published in Biomaterials on June 29, 2017

Authors

Anthony J Berger1, Kelsey M Linsmeier1, Pamela K Kreeger2, Kristyn S Masters3

Author Affiliations

1: Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, United States.
2: Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, United States; Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States; Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States. Electronic address: kreeger@wisc.edu.
3: Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, United States; Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States; Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States; Department of Materials Science and Engineering, University of Wisconsin-Madison, Madison, WI, United States. Electronic address: kmasters@wisc.edu.

Articles cited by this

Hallmarks of cancer: the next generation. Cell (2011) 140.01

Fiji: an open-source platform for biological-image analysis. Nat Methods (2012) 56.92

Matrix elasticity directs stem cell lineage specification. Cell (2006) 43.00

Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci U S A (1997) 19.46

Tensional homeostasis and the malignant phenotype. Cancer Cell (2005) 19.30

Cell movement is guided by the rigidity of the substrate. Biophys J (2000) 13.84

Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. Cell Motil Cytoskeleton (2005) 10.20

Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments. J Cell Biol (2004) 7.38

Force sensing by mechanical extension of the Src family kinase substrate p130Cas. Cell (2006) 7.35

Basement membranes: structure, assembly and role in tumour angiogenesis. Nat Rev Cancer (2003) 6.65

Collagen density promotes mammary tumor initiation and progression. BMC Med (2008) 5.13

Aligned collagen is a prognostic signature for survival in human breast carcinoma. Am J Pathol (2011) 3.70

Matrix density-induced mechanoregulation of breast cell phenotype, signaling and gene expression through a FAK-ERK linkage. Oncogene (2009) 3.17

Force fluctuations within focal adhesions mediate ECM-rigidity sensing to guide directed cell migration. Cell (2012) 2.87

A cancer cell metalloprotease triad regulates the basement membrane transmigration program. Genes Dev (2006) 2.78

Photoinitiated polymerization of PEG-diacrylate with lithium phenyl-2,4,6-trimethylbenzoylphosphinate: polymerization rate and cytocompatibility. Biomaterials (2009) 2.65

The effect of matrix density on the regulation of 3-D capillary morphogenesis. Biophys J (2007) 2.37

3D reconstruction of histological sections: Application to mammary gland tissue. Microsc Res Tech (2010) 2.26

The use of poly(ethylene glycol) hydrogels to investigate the impact of ECM chemistry and mechanics on smooth muscle cells. Biomaterials (2006) 2.23

Non-linear elasticity of extracellular matrices enables contractile cells to communicate local position and orientation. PLoS One (2009) 2.06

Matrix rigidity regulates cancer cell growth and cellular phenotype. PLoS One (2010) 1.70

Functional Human Vascular Network Generated in Photocrosslinkable Gelatin Methacrylate Hydrogels. Adv Funct Mater (2012) 1.63

Biased cell migration of fibroblasts exhibiting contact guidance in oriented collagen gels. Ann Biomed Eng (1995) 1.58

Guiding cell migration in 3D: a collagen matrix with graded directional stiffness. Cell Motil Cytoskeleton (2009) 1.49

MT1-MMP- and Cdc42-dependent signaling co-regulate cell invasion and tunnel formation in 3D collagen matrices. J Cell Sci (2009) 1.33

Changes in extracellular matrix (ECM) and ECM-associated proteins in the metastatic progression of prostate cancer. Reprod Biol Endocrinol (2004) 1.29

Matrix density mediates polarization and lumen formation of endothelial sprouts in VEGF gradients. Lab Chip (2010) 1.25

Effects of the mechanical properties of collagen gel on the in vitro formation of microvessel networks by endothelial cells. Tissue Eng (2007) 1.24

Biophysical control of invasive tumor cell behavior by extracellular matrix microarchitecture. Biomaterials (2012) 1.24

Tuning three-dimensional collagen matrix stiffness independently of collagen concentration modulates endothelial cell behavior. Acta Biomater (2012) 1.22

The role of changes in extracellular matrix of cartilage in the presence of inflammation on the pathology of osteoarthritis. Biomed Res Int (2013) 1.18

Stiff collagen matrices increase tumorigenic prolactin signaling in breast cancer cells. J Biol Chem (2013) 1.15

Human breast cancer invasion and aggression correlates with ECM stiffening and immune cell infiltration. Integr Biol (Camb) (2015) 1.13

Dynamic stiffening of poly(ethylene glycol)-based hydrogels to direct valvular interstitial cell phenotype in a three-dimensional environment. Biomaterials (2015) 1.08

Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol (2015) 1.07

Automated quantification of aligned collagen for human breast carcinoma prognosis. J Pathol Inform (2014) 1.03

Biomimetic hydrogels for chondrogenic differentiation of human mesenchymal stem cells to neocartilage. Biomaterials (2010) 1.01

Matrix rigidity regulates cancer cell growth by modulating cellular metabolism and protein synthesis. PLoS One (2012) 0.99

Bone regeneration using an alpha 2 beta 1 integrin-specific hydrogel as a BMP-2 delivery vehicle. Biomaterials (2014) 0.99

A cell-instructive hydrogel to regulate malignancy of 3D tumor spheroids with matrix rigidity. Biomaterials (2011) 0.99

Cell-mediated fibre recruitment drives extracellular matrix mechanosensing in engineered fibrillar microenvironments. Nat Mater (2015) 0.99

Extracellular matrix density regulates the rate of neovessel growth and branching in sprouting angiogenesis. PLoS One (2014) 0.95

Modelling cell migration strategies in the extracellular matrix. J Math Biol (2008) 0.93

Dynamic changes in myocardial matrix and relevance to disease: translational perspectives. Circ Res (2014) 0.92

Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration. Curr Protoc Cell Biol (2010) 0.90

Glycated collagen alters endothelial cell actin alignment and nitric oxide release in response to fluid shear stress. J Biomech (2011) 0.90

Functionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms. Nat Protoc (2016) 0.88

The spatial-temporal characteristics of type I collagen-based extracellular matrix. Soft Matter (2014) 0.84

Endothelial cell spheroids as a versatile tool to study angiogenesis in vitro. FASEB J (2015) 0.83

Tumor Cell Invasion Can Be Blocked by Modulators of Collagen Fibril Alignment That Control Assembly of the Extracellular Matrix. Cancer Res (2016) 0.83

Angiogenic responses are enhanced in mechanically and microscopically characterized, microbial transglutaminase crosslinked collagen matrices with increased stiffness. Acta Biomater (2013) 0.83

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting. J Vis Exp (2015) 0.83

In vitro microtumors provide a physiologically predictive tool for breast cancer therapeutic screening. PLoS One (2015) 0.83

The Mechanobiology of Aging. Annu Rev Biomed Eng (2015) 0.81

Fibrous nonlinear elasticity enables positive mechanical feedback between cells and ECMs. Proc Natl Acad Sci U S A (2016) 0.80

Characterization of methacrylated type-I collagen as a dynamic, photoactive hydrogel. Biointerphases (2012) 0.80

Colorful protein-based fluorescent probes for collagen imaging. PLoS One (2014) 0.79

Tunable collagen I hydrogels for engineered physiological tissue micro-environments. PLoS One (2015) 0.77

Stiffened Extracellular Matrix and Signaling from Stromal Fibroblasts via Osteoprotegerin Regulate Tumor Cell Invasion in a 3-D Tumor in Situ Model. Cancer Microenviron (2016) 0.75