Preparation of interstitial lung cells by enzymatic digestion of tissue slices: preliminary characterization by morphology and performance in functional assays.

PubWeight™: 2.02‹?› | Rank: Top 2%

🔗 View Article (PMC 1454850)

Published in Immunology on January 01, 1985

Authors

P G Holt, A Degebrodt, T Venaille, C O'Leary, K Krska, J Flexman, H Farrell, G Shellam, P Young, J Penhale

Articles citing this

MHC class II antigen-bearing dendritic cells in pulmonary tissues of the rat. Regulation of antigen presentation activity by endogenous macrophage populations. J Exp Med (1988) 3.21

Host cellular immune response to pneumococcal lung infection in mice. Infect Immun (2000) 2.74

Down-regulation of immune responses in the lower respiratory tract: the role of alveolar macrophages. Clin Exp Immunol (1986) 2.43

T cell activation by antigen-presenting cells from lung tissue digests: suppression by endogenous macrophages. Clin Exp Immunol (1985) 2.16

Immune and inflammatory function in cigarette smokers. Thorax (1987) 2.07

Localization of T cells, macrophages and dendritic cells in rat respiratory tract tissue: implications for immune function studies. Immunology (1987) 1.73

Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells. J Exp Med (1990) 1.65

Novel features of the respiratory tract T-cell response to influenza virus infection: lung T cells increase expression of gamma interferon mRNA in vivo and maintain high levels of mRNA expression for interleukin-5 (IL-5) and IL-10. J Virol (1994) 1.62

Extraction of immune and inflammatory cells from human lung parenchyma: evaluation of an enzymatic digestion procedure. Clin Exp Immunol (1986) 1.57

Pulmonary inflammation induced by repeated inhalations of beta(1,3)-D-glucan and endotoxin. Int J Exp Pathol (1994) 1.50

Control of murine cytomegalovirus in the lungs: relative but not absolute immunodominance of the immediate-early 1 nonapeptide during the antiviral cytolytic T-lymphocyte response in pulmonary infiltrates. J Virol (1998) 1.38

Inflammatory response following intranasal infection with Mycobacterium avium complex: role of T-cell subsets and gamma interferon. Infect Immun (1995) 1.34

Development of the airway intraepithelial dendritic cell network in the rat from class II major histocompatibility (Ia)-negative precursors: differential regulation of Ia expression at different levels of the respiratory tract. J Exp Med (1994) 1.28

Regulation of T-cell function in lung tissue by pulmonary alveolar macrophages. Immunology (1993) 1.27

Endogenous interleukin-12 is involved in resistance of mice to Mycobacterium avium complex infection. Infect Immun (1995) 1.27

Limiting-dilution analysis of T cells extracted from solid human lung tissue: comparison of precursor frequencies for proliferative responses and lymphokine production between lung and blood T cells from individual donors. Immunology (1988) 1.21

Inhalation of (1-->3)-beta-D-glucan causes airway eosinophilia. Mediators Inflamm (2001) 1.19

Lung lymphocyte elimination by apoptosis in the murine response to intratracheal particulate antigen. J Clin Invest (1997) 1.05

Blocking macrophage leukotriene b4 prevents endothelial injury and reverses pulmonary hypertension. Sci Transl Med (2013) 1.05

(1-->3)-beta-D-glucan and endotoxin modulate immune response to inhaled allergen. Mediators Inflamm (1998) 1.00

FcR+/- subsets of Ia+ pulmonary dendritic cells in the rat display differences in their abilities to provide accessory co-stimulation for naive (OX-22+) and sensitized (OX-22-) T cells. Am J Pathol (1993) 0.98

Control of cytomegalovirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rests primarily on recipient-derived CD8 T cells. J Virol (1998) 0.96

Regulation of T-cell proliferative responses by cells from solid lung tissue of M. tuberculosis-infected mice. Immunology (1991) 0.93

Selective attrition of non-recirculating T cells during normal passage through the lung vascular bed. Immunology (1990) 0.92

Low-molecular-weight heparin reduces hyperoxia-augmented ventilator-induced lung injury via serine/threonine kinase-protein kinase B. Respir Res (2011) 0.90

The surface phenotypic characterization of lung macrophages in C3H/HeJ mice. Immunology (1991) 0.89

A clonal analysis of lung T cells derived by bronchoalveolar lavage of healthy individuals. Immunology (1992) 0.88

Response of lung gammadelta T cells to experimental sepsis in mice. Immunology (2004) 0.88

Cytochrome c oxidase subunit 4 isoform 2-knockout mice show reduced enzyme activity, airway hyporeactivity, and lung pathology. FASEB J (2012) 0.87

Mechanisms, measurement, and significance of lung macrophage function. Environ Health Perspect (1992) 0.82

Activity of lung neutrophils and matrix metalloproteinases in cyclophosphamide-treated mice with experimental sepsis. Int J Exp Pathol (2004) 0.79

Recurring BALB/c mouse lung inflammatory responses to episodic allergen exposure. J Toxicol Environ Health A (2013) 0.77

Selected new developments in asbestos immunotoxicity. Environ Health Perspect (1998) 0.77

Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody. Proc Natl Acad Sci U S A (2017) 0.75

Articles cited by this

A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol (1973) 45.44

Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell (1977) 8.46

Two subsets of rat T lymphocytes defined with monoclonal antibodies. Eur J Immunol (1980) 5.98

Origin, Kinetics, and characteristics of pulmonary macrophages in the normal steady state. J Exp Med (1979) 3.57

Cell number and cell characteristics of the normal human lung. Am Rev Respir Dis (1982) 2.54

The origin, kinetics, and characteristics of the Kupffer cells in the normal steady state. J Exp Med (1978) 2.29

Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies. Immunology (1982) 2.15

Natural killer (NK) cell activity in the rat. I. Isolation and characterization of the effector cells. J Immunol (1981) 2.12

An enzymatic method for preparation of dissociated murine Peyer's patch cells enriched for macrophages. J Immunol Methods (1982) 2.06

Alveolar macrophages. I. A simple technique for the preparation of high numbers of viable alveolar macrophages from small laboratory animals. J Immunol Methods (1979) 1.98

Pathways of clearance in mouse lungs exposed to iron oxide aerosols. Anat Rec (1975) 1.95

The accessory cell function of human alveolar macrophages in specific T cell proliferation. J Immunol (1984) 1.81

The lymphocyte surface. I. Relation between Fc receptors, C'3 receptors and surface immunoglobulin. Proc R Soc Lond B Biol Sci (1974) 1.80

Natural killer cells in mouse lung: surface phenotype, target preference, and response to local influenza virus infection. J Immunol (1983) 1.56

Release of platelet-activating factor (PAF-acether) from alveolar macrophages by the calcium ionophore A23187 and phagocytosis. Eur J Clin Invest (1980) 1.50

Lymphocyte studies in asymptomatic cigarette smokers. A comparison between lung and peripheral blood. Am Rev Respir Dis (1977) 1.41

Lung cell reactions after inhalation of bacterial lipopolysaccharides. Eur J Respir Dis (1982) 1.38

Alveolar macrophages. III. Studies on the mechanisms of inhibition of T-cell proliferation. Immunology (1979) 1.29

Alveolar macrophages. VI. Regulation of alveolar macrophage-mediated suppression of lymphocyte proliferation by a putative T cell. Immunology (1981) 1.11

Augmentation of cell-mediated cytotoxicity to a rat lymphoma. I. Stimulation of non-T-cell cytotoxicity in vivo by tumour cells. Int J Cancer (1979) 1.10

Alveolar macrophages. V. Comparative studies on the antigen presentation activity of guinea-pig and rat alveolar macrophages. Immunology (1980) 1.09

Alveolitis induced by influenza virus. Am Rev Respir Dis (1983) 1.01

The capacity of murine alveolar macrophages to stimulate antigen-dependent T-lymphocyte activation and proliferation. Cell Immunol (1983) 0.99

Alveolar macrophages: functional heterogeneity within macrophage populations from rat lung. Aust J Exp Biol Med Sci (1982) 0.99

Target-effector interactions in the rat natural killer cell system. I. The measurement of cytotoxicity at the single cell level. Eur J Immunol (1982) 0.92

Procoagulant activity of rabbit alveolar macrophages. Am Rev Respir Dis (1983) 0.90

Characterization of a postlavage, in situ pulmonary macrophage population. J Cell Physiol (1982) 0.85

Articles by these authors

Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. Science (1998) 18.23

beta Blockade after myocardial infarction: systematic review and meta regression analysis. BMJ (1999) 5.66

Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted. J Virol (1992) 5.18

A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J Immunol Methods (1983) 4.39

Development of allergen-specific T-cell memory in atopic and normal children. Lancet (1999) 4.34

Roles for Nkx3.1 in prostate development and cancer. Genes Dev (1999) 4.30

The natural history of Hendra and Nipah viruses. Microbes Infect (2001) 3.77

A Polymorphism* in the 5' flanking region of the CD14 gene is associated with circulating soluble CD14 levels and with total serum immunoglobulin E. Am J Respir Cell Mol Biol (1999) 3.42

A comparison of the reliability of self-reported drug use and sexual behaviors using computer-assisted versus face-to-face interviewing. AIDS Educ Prev (2000) 3.02

Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity. J Exp Med (1998) 2.85

Midwifery and body fluid contamination. BMJ (1992) 2.81

Structural basis for activation of the titin kinase domain during myofibrillogenesis. Nature (1998) 2.80

Prediction of the progression of joint space narrowing in osteoarthritis of the knee by bone scintigraphy. Ann Rheum Dis (1993) 2.78

Regulation of IgE responses to inhaled antigen in mice by antigen-specific gamma delta T cells. Science (1994) 2.66

Stromal estrogen receptors mediate mitogenic effects of estradiol on uterine epithelium. Proc Natl Acad Sci U S A (1997) 2.54

Transplacental priming of the human immune system to environmental allergens: universal skewing of initial T cell responses toward the Th2 cytokine profile. J Immunol (1998) 2.53

Rapid dendritic cell recruitment is a hallmark of the acute inflammatory response at mucosal surfaces. J Exp Med (1994) 2.52

Studies on the density, distribution, and surface phenotype of intraepithelial class II major histocompatibility complex antigen (Ia)-bearing dendritic cells (DC) in the conducting airways. J Exp Med (1991) 2.51

A mouse cytomegalovirus glycoprotein retains MHC class I complexes in the ERGIC/cis-Golgi compartments. Immunity (1997) 2.49

A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat. Genome Res (1999) 2.49

Down-regulation of immune responses in the lower respiratory tract: the role of alveolar macrophages. Clin Exp Immunol (1986) 2.43

An immunoepidemiological approach to asthma: identification of in-vitro T-cell response patterns associated with different wheezing phenotypes in children. Lancet (2005) 2.39

Brown adipose tissue in the parametrial fat pad of the mouse. FEBS Lett (1984) 2.36

Association between breast feeding and asthma in 6 year old children: findings of a prospective birth cohort study. BMJ (1999) 2.34

Effect of cigarette smoking on primary and secondary humoral responses of mice. Nature (1973) 2.27

Macrophage-derived nitric oxide regulates T cell activation via reversible disruption of the Jak3/STAT5 signaling pathway. J Immunol (1998) 2.25

Vitamin D and atopy and asthma phenotypes in children: a longitudinal cohort study. Eur Respir J (2011) 2.21

Dendritic cells are recruited into the airway epithelium during the inflammatory response to a broad spectrum of stimuli. J Exp Med (1996) 2.19

T cell activation by antigen-presenting cells from lung tissue digests: suppression by endogenous macrophages. Clin Exp Immunol (1985) 2.16

The endurance shuttle walking test: a responsive measure in pulmonary rehabilitation for COPD patients. Chron Respir Dis (2006) 2.09

Association between antenatal cytokine production and the development of atopy and asthma at age 6 years. Lancet (2003) 2.09

Immune and inflammatory function in cigarette smokers. Thorax (1987) 2.07

Signaling through the stromal epidermal growth factor receptor is necessary for mammary ductal development. Development (1999) 2.04

Inhibition of the immunosuppressive activity of resident pulmonary alveolar macrophages by granulocyte/macrophage colony-stimulating factor. J Exp Med (1993) 2.03

The natural immune response to inhaled soluble protein antigens involves major histocompatibility complex (MHC) class I-restricted CD8+ T cell-mediated but MHC class II-restricted CD4+ T cell-dependent immune deviation resulting in selective suppression of immunoglobulin E production. J Exp Med (1993) 2.02

Inhibition of specific IgE responses in mice by pre-exposure to inhaled antigen. Immunology (1981) 2.01

Ambulance-user analysis in an accident and emergency department. Ir Med J (1987) 1.99

A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface. EMBO J (1997) 1.98

A validation study on the use of intra-operative video recording as an objective assessment tool for core ENT surgery. Clin Otolaryngol (2014) 1.98

Alveolar macrophages. I. A simple technique for the preparation of high numbers of viable alveolar macrophages from small laboratory animals. J Immunol Methods (1979) 1.98

Are accelerometers a useful tool for measuring disease activity in children with eczema? Validity, responsiveness to change, and acceptability of use in a clinical trial setting. Br J Dermatol (2012) 1.97

Benefits of pravastatin on cardiovascular events and mortality in older patients with coronary heart disease are equal to or exceed those seen in younger patients: Results from the LIPID trial. Ann Intern Med (2001) 1.97

Characterization of sheep afferent lymph dendritic cells and their role in antigen carriage. J Exp Med (1989) 1.97

Role of microbial burden in aetiology of allergy and asthma. Lancet (1999) 1.92

Characterization of a novel TNF-like ligand and recently described TNF ligand and TNF receptor superfamily genes and their constitutive and inducible expression in hematopoietic and non-hematopoietic cells. Gene (1997) 1.88

Crystal structure of the alpha-actinin rod reveals an extensive torsional twist. Structure (2001) 1.85

Structure of the alpha-actinin rod: molecular basis for cross-linking of actin filaments. Cell (1999) 1.80

Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation. Clin Exp Allergy (2007) 1.79

TH2-polarized immunological memory to inhalant allergens in atopics is established during infancy and early childhood. Clin Exp Allergy (1997) 1.77

Giant axonal neuropathy (GAN): case report and two novel mutations in the gigaxonin gene. Neurology (2002) 1.77

Allergic respiratory disease: strategic targets for primary prevention during childhood. Thorax (1997) 1.76

Induction of IgE-secreting cells and IgE isotype-specific suppressor T cells in the respiratory lymph nodes of rats in response to antigen inhalation. Cell Immunol (1985) 1.73

forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster. Genetics (1994) 1.73

Elucidation of a role for stromal steroid hormone receptors in mammary gland growth and development using tissue recombinants. J Mammary Gland Biol Neoplasia (1997) 1.73

Comorbidity of migraine and restless legs syndrome--a case-control study. Cephalalgia (2007) 1.71

Systematic review of topical treatments for fungal infections of the skin and nails of the feet. BMJ (1999) 1.70

Modification of the inflammatory response to allergen challenge after exposure to bacterial lipopolysaccharide. Am J Respir Cell Mol Biol (2000) 1.70

The hydrophobic effect contributes to polyubiquitin chain recognition. Biochemistry (1998) 1.68

Association between early sexual abuse and adult HIV-risky sexual behaviors among community-recruited women. Child Abuse Negl (2001) 1.66

Febrile respiratory illnesses in infancy and atopy are risk factors for persistent asthma and wheeze. Eur Respir J (2011) 1.65

Stromal progesterone receptors mediate the inhibitory effects of progesterone on estrogen-induced uterine epithelial cell deoxyribonucleic acid synthesis. Endocrinology (1998) 1.64

Maternal fish oil supplementation in pregnancy reduces interleukin-13 levels in cord blood of infants at high risk of atopy. Clin Exp Allergy (2003) 1.61

Breast feeding and respiratory morbidity in infancy: a birth cohort study. Arch Dis Child (2003) 1.60

Regulation of immune response to inhaled antigen by alveolar macrophages: differential effects of in vivo alveolar macrophage elimination on the induction of tolerance vs. immunity. Eur J Immunol (1991) 1.59

Th2-associated immunity to bacteria in teenagers and susceptibility to asthma. Eur Respir J (2010) 1.58

Extraction of immune and inflammatory cells from human lung parenchyma: evaluation of an enzymatic digestion procedure. Clin Exp Immunol (1986) 1.57

Selective inhibition of T cell proliferation but not expression of effector function by human alveolar macrophages. Thorax (1997) 1.56

Environmentally induced changes in immunological function: acute and chronic effects of inhalation of tobacco smoke and other atmospheric contaminants in man and experimental animals. Bacteriol Rev (1977) 1.51

Effect of meal viscosity and nutrients on satiety, intragastric dilution, and emptying assessed by MRI. Am J Physiol Gastrointest Liver Physiol (2001) 1.50

Mechanical and constitutional risk factors for symptomatic knee osteoarthritis: differences between medial tibiofemoral and patellofemoral disease. J Rheumatol (1994) 1.48

Functional characteristics of the veiled cells in afferent lymph from the rat intestine. Immunology (1986) 1.48

Increased prevalence of obstructive sleep apnoea in patients with Charcot-Marie-Tooth disease: a case control study. J Neurol Neurosurg Psychiatry (2008) 1.47

Rapid dendritic cell recruitment to the bronchial mucosa of patients with atopic asthma in response to local allergen challenge. Thorax (2001) 1.47

A multicenter evaluation of total intravenous anesthesia with remifentanil and propofol for elective inpatient surgery. Anesth Analg (1996) 1.44

Airways inflammation, atopy, and (1--> 3)-beta-D-glucan exposures in two schools. Am J Respir Crit Care Med (1998) 1.43

A randomized trial of Evidence-based OutReach (EBOR): rationale and design. Control Clin Trials (1999) 1.42

Heterogeneity in diphtheria-tetanus-acellular pertussis vaccine-specific cellular immunity during infancy: relationship to variations in the kinetics of postnatal maturation of systemic th1 function. J Infect Dis (2001) 1.42

Fission yeast cdc25 is a cell-cycle regulated protein. Biochem Biophys Res Commun (1990) 1.41

Extracts from "Clinical evidence": Athlete's foot and fungally infected toenails. BMJ (2001) 1.41

Alveolar macrophages. II. Inhibition of lymphocyte proliferation by purified macrophages from rat lung. Immunology (1979) 1.40

Efficacy of camptothecin in progressive multifocal leucoencephalopathy. Lancet (1997) 1.40

Maternal smoking in pregnancy alters neonatal cytokine responses. Allergy (2003) 1.40

Hepatitis C virus-associated hepatitis following treatment of HIV-infected patients with HIV protease inhibitors: an immune restoration disease? AIDS (1998) 1.38

Nipple pain. J Obstet Gynecol Neonatal Nurs (1994) 1.38

Plasma progesterone concentrations in llamas. Vet Rec (1990) 1.38

A first exon-encoded domain of E1A sufficient for posttranslational modification, nuclear-localization, and induction of adenovirus E3 promoter expression in Xenopus oocytes. Proc Natl Acad Sci U S A (1985) 1.36

Cellular and humoral immune response in progressive multifocal leukoencephalopathy. Ann Neurol (2001) 1.35

The effects of respiratory infections, atopy, and breastfeeding on childhood asthma. Eur Respir J (2002) 1.35

Sex hormone-induced carcinogenesis in Rb-deficient prostate tissue. Cancer Res (2000) 1.35

Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion. Immunology (1995) 1.34

pKa values and the pH dependent stability of the N-terminal domain of L9 as probes of electrostatic interactions in the denatured state. Differentiation between local and nonlocal interactions. Biochemistry (1999) 1.34

Acute myelogenous leukemia with leukemia cutis. Eighteen cases seen between 1969 and 1986. Cancer (1989) 1.33

Mesenchymal reprogramming of adult human epithelial differentiation. Differentiation (1999) 1.32

cdc2 and the regulation of mitosis: six interacting mcs genes. Genetics (1989) 1.32

The murine cytomegalovirus chemokine homolog, m131/129, is a determinant of viral pathogenicity. J Virol (1999) 1.32

Keratinocyte growth factor functions in epithelial induction during seminal vesicle development. Proc Natl Acad Sci U S A (1994) 1.31