Published in Anal Chem on February 01, 1970
Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci U S A (1968) 10.63
The use of the avidin-biotin complex as a tool in molecular biology. Methods Biochem Anal (1980) 6.48
Antibodies against a synthetic peptide as a probe for the kinase activity of the avian EGF receptor and v-erbB protein. Cell (1985) 4.02
CTL induction by a tumour-associated antigen octapeptide derived from a murine lung carcinoma. Nature (1994) 3.70
Three-dimensional structures of avidin and the avidin-biotin complex. Proc Natl Acad Sci U S A (1993) 3.02
Distribution of end-to-end distances of oligopeptides in solution as estimated by energy transfer. Proc Natl Acad Sci U S A (1975) 2.50
Affinity chromatography of bovine pancreatic ribonuclease A. Eur J Biochem (1969) 2.11
A 25-kD inhibitor of actin polymerization is a low molecular mass heat shock protein. J Cell Biol (1991) 2.08
All-D-magainin: chirality, antimicrobial activity and proteolytic resistance. FEBS Lett (1990) 2.05
Selective oxidation of methionine residues in proteins. Biochemistry (1975) 2.03
Regression of established murine carcinoma metastases following vaccination with tumour-associated antigen peptides. Nat Med (1995) 1.99
Introduction to avidin-biotin technology. Methods Enzymol (1990) 1.96
The binding site of acetylcholine receptor as visualized in the X-Ray structure of a complex between alpha-bungarotoxin and a mimotope peptide. Neuron (2001) 1.96
Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 1. General introduction. J Biol Chem (1976) 1.91
Protein biotinylation. Methods Enzymol (1990) 1.89
Covalent coupling of nucleotides to agarose for affinity chromatography. Biochim Biophys Acta (1973) 1.84
A general method for the specific isolation of peptides containing modified residues, using insoluble antibody columns. Biochemistry (1971) 1.70
Irreversible inhibition of biotin transport in yeast by biotinyl-p-nitrophenyl ester. Proc Natl Acad Sci U S A (1971) 1.66
Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Appl Environ Microbiol (1995) 1.57
The avidin-biotin complex in affinity cytochemistry. Methods Enzymol (1979) 1.53
A new approach (cyano-transfer) for cyanogen bromide activation of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives. Biochem Biophys Res Commun (1982) 1.52
The covalent binding of daunomycin and adriamycin to antibodies, with retention of both drug and antibody activities. Cancer Res (1975) 1.48
Cyanylation of sulfhydryl groups by 2-nitro-5-thiocyanobenzoic acid. High-yield modification and cleavage of peptides at cysteine residues. Biochemistry (1974) 1.44
Selective chemical cleavage of tryptophanyl peptide bonds by oxidative chlorination with N-chlorosuccinimide. Biochemistry (1976) 1.43
Single-step purification of avidine from egg white by affinity chromatography on biocytin-Sepharose columns. Biochem Biophys Res Commun (1968) 1.43
Avidin-biotin technology ten years on: has it lived up to its expectations? Trends Biochem Sci (1989) 1.42
Antigenicity and immunogenicity of an intracellular delivery system of major histocompatibility complex class I epitopes that bypasses proteasome processing. J Immunother (2001) 1.41
Affinity cytochemistry: the localization of lectin and antibody receptors on erythrocytes via the avidin-biotin complex. FEBS Lett (1976) 1.41
Enhancement of phagocytosis - a newly found activity of substance P residing in its N-terminal tetrapeptide sequence. Biochem Biophys Res Commun (1980) 1.39
Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining. Anal Biochem (1988) 1.38
Insolubilized biotin for the purification of avidin. Methods Enzymol (1974) 1.36
Tuftsin (an Ig-associated tetrapeptide) triggers the immunogenic function of macrophages: implications for activation of programmed cells. Proc Natl Acad Sci U S A (1978) 1.33
Letter: Levulinic esters. An alcohol protecting group applicable to some nucleosides. J Am Chem Soc (1975) 1.31
A sensitive enzyme assay for biotin, avidin, and streptavidin. Anal Biochem (1986) 1.29
Structure-function studies of polymyxin B nonapeptide: implications to sensitization of gram-negative bacteria. J Med Chem (2000) 1.25
Augmentation of the antibacterial activity of magainin by positive-charge chain extension. Antimicrob Agents Chemother (1992) 1.25
Antibodies to synthetic peptides as probes for the binding site on the alpha subunit of the acetylcholine receptor. Proc Natl Acad Sci U S A (1985) 1.25
A general method for the isolation of labelled peptides from affinity-labelled proteins. Biochem Biophys Res Commun (1970) 1.24
Application of avidin-biotin technology to affinity-based separations. J Chromatogr (1990) 1.22
Biotin binding to avidin. Oligosaccharide side chain not required for ligand association. Biochem J (1987) 1.22
Applications of avidin-biotin technology: literature survey. Methods Enzymol (1990) 1.20
The use of carbodiimides in the preparation of immunizing conjugates. Methods Enzymol (1980) 1.20
Studies on the biotin-binding site of streptavidin. Tryptophan residues involved in the active site. Biochem J (1988) 1.18
Sodium dodecyl sulfate-polyacrylamide gel electrophoretic method for assessing the quaternary state and comparative thermostability of avidin and streptavidin. Electrophoresis (1996) 1.18
Affinity chromatography. Methods Enzymol (1984) 1.18
The mode of action of allicin: its ready permeability through phospholipid membranes may contribute to its biological activity. Biochim Biophys Acta (2000) 1.17
The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins. Biochim Biophys Acta (1998) 1.17
An electrophilic mechanism in the chymotrypsin-catalyzed hydrolysis of anilide substrates. J Am Chem Soc (1965) 1.16
Preparation of ferritin-avidin conjugates by reductive alkylation for use in electron microscopic cytochemistry. J Histochem Cytochem (1976) 1.16
N-terminal modifications of Polymyxin B nonapeptide and their effect on antibacterial activity. Peptides (2001) 1.14
Tryptophan residues of cholera toxin and its A and B protomers. Intrinsic fluorescence and solute quenching upon interacting with the ganglioside GM1, oligo-GM1, or dansylated oligo-GM1. J Biol Chem (1981) 1.14
The quaternary structure of streptavidin in urea. J Biol Chem (1991) 1.14
Reversibility of biotin-binding by selective modification of tyrosine in avidin. Biochem J (1996) 1.14
Studies on the biotin-binding site of avidin. Tryptophan residues involved in the active site. Biochem J (1988) 1.13
MHC class I-restricted epitope spreading in the context of tumor rejection following vaccination with a single immunodominant CTL epitope. Eur J Immunol (1999) 1.13
Biotin induces tetramerization of a recombinant monomeric avidin. A model for protein-protein interactions. J Biol Chem (2000) 1.11
Haloacetyl derivatives. Methods Enzymol (1977) 1.11
Characterization of an inhibitor of actin polymerization in vinculin-rich fraction of turkey gizzard smooth muscle. Eur J Biochem (1988) 1.11
An improved method for the single-step purification of streptavidin. J Biochem Biophys Methods (1986) 1.11
Biocytin hydrazide--a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology. Anal Biochem (1988) 1.10
Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain. FEBS Lett (1999) 1.10
Studies on the biotin-binding site of avidin. Lysine residues involved in the active site. Biochem J (1987) 1.10
Structure-activity relationships of 4-(phenylethynyl)-6-phenyl-1,4-dihydropyridines as highly selective A3 adenosine receptor antagonists. J Med Chem (1997) 1.09
An antagonist to vasoactive intestinal peptide affects cellular functions in the central nervous system. J Pharmacol Exp Ther (1991) 1.09
Tissue distribution of avidin and streptavidin injected to mice. Effect of avidin carbohydrate, streptavidin truncation and exogenous biotin. Eur J Biochem (1990) 1.09
Structure-function studies of cholera toxin and its A and B protomers. Modification of tryptophan residues. J Biol Chem (1981) 1.09
A beta -hairpin structure in a 13-mer peptide that binds alpha -bungarotoxin with high affinity and neutralizes its toxicity. Proc Natl Acad Sci U S A (2001) 1.07
Recombinant NeutraLite avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties. FEBS Lett (2000) 1.06
Selective cyanylation of sulfhydryl groups. J Am Chem Soc (1970) 1.06
Tuftsin and some analogs: synthesis and interaction with human polymorphonuclear leukocytes. Biochim Biophys Acta (1977) 1.06
Analysis of ligand binding to the synthetic dodecapeptide 185-196 of the acetylcholine receptor alpha subunit. Proc Natl Acad Sci U S A (1986) 1.05
Decreased tuftsin concentrations in patients who have undergone splenectomy. Br Med J (1977) 1.05
The functional association of polymyxin B with bacterial lipopolysaccharide is stereospecific: studies on polymyxin B nonapeptide. Biochemistry (2000) 1.05
Foreword and introduction to the book (strept)avidin-biotin system. Biomol Eng (1999) 1.05
Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site. Biochem J (1990) 1.05
Mapping of Escherichia coli ribosomal components involved in peptidyl transferase activity. Proc Natl Acad Sci U S A (1973) 1.04
Direct visualization of redistribution and capping of fluorescent gangliosides on lymphocytes. J Cell Biol (1984) 1.04
The mode of adsorption of proteins to aliphatic and aromatic amines coupled to cyanogen bromide-activated agarose. Biochim Biophys Acta (1974) 1.04
Activity-dependent neurotrophic factor: structure-activity relationships of femtomolar-acting peptides. J Pharmacol Exp Ther (1998) 1.04
Postsecretory modifications of streptavidin. Biochem J (1989) 1.03
Biotin-containing reagents. Methods Enzymol (1990) 1.03
Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos. Nature (1993) 1.03
Structure-function studies of amphiphilic antibacterial peptides. J Med Chem (1993) 1.02
Studies on the biotin-binding site of avidin. Minimized fragments that bind biotin. Biochem J (1991) 1.02
The action of staphylococcal nuclease on synthetic substrates. Biochemistry (1969) 1.02
Insulin-like effects of vanadium: basic and clinical implications. J Inorg Biochem (2000) 1.01
Effect of purified allicin, the major ingredient of freshly crushed garlic, on cancer cell proliferation. Nutr Cancer (2000) 1.01
Monofunctional substrates of polynucleotide phosphorylase. The monoaddition of 2'(3')-O-isovaleryl-nucleoside diphosphate to an initiator oligonucleotide. Eur J Biochem (1971) 1.01
Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem Biophys Res Commun (1987) 1.00
Streptavidin contains an RYD sequence which mimics the RGD receptor domain of fibronectin. Biochem Biophys Res Commun (1990) 1.00
Superactive lipophilic peptides discriminate multiple vasoactive intestinal peptide receptors. J Pharmacol Exp Ther (1995) 1.00
Learning impairment following intracerebral administration of the HIV envelope protein gp120 or a VIP antagonist. Brain Res (1992) 1.00
Selective cleavage and modification of peptides and proteins. Adv Protein Chem (1970) 1.00
Vasoactive intestinal peptide potentiates sexual behavior: inhibition by novel antagonist. Endocrinology (1989) 1.00
A peptide composed of tandem analogs of two myasthenogenic T cell epitopes interferes with specific autoimmune responses. Proc Natl Acad Sci U S A (1997) 0.99
Prevention of experimental antiphospholipid syndrome and endothelial cell activation by synthetic peptides. Proc Natl Acad Sci U S A (1999) 0.99
Neuroprotective strategy for Alzheimer disease: intranasal administration of a fatty neuropeptide. Proc Natl Acad Sci U S A (1996) 0.99
The reaction of derivatives of tryptophan, tryptamine, and other indoles with 2-hydroxy-5-nitrobenzyl bromide (Koshland's reagent). J Am Chem Soc (1968) 0.99
A spectrophotometric assay for allicin and alliinase (Alliin lyase) activity: reaction of 2-nitro-5-thiobenzoate with thiosulfinates. Anal Biochem (1998) 0.99
Novel breast-tumor-associated MUC1-derived peptides: characterization in Db-/- x beta2 microglobulin (beta2m) null mice transgenic for a chimeric HLA-A2.1/Db-beta2 microglobulin single chain. Int J Cancer (2000) 0.99