Adenylate cyclase assay with [alpha-32P]ATP as substrate. A simple modification for lowering blanks.

PubWeight™: 0.75‹?›

🔗 View Article (PMID 626360)

Published in Anal Biochem on January 01, 1978

Authors

R Counis, S Mongongu

Articles by these authors

Expression of gonadotropin-releasing hormone (GnRH) receptor gene is altered by GnRH agonist desensitization in a manner similar to that of gonadotropin beta-subunit genes in normal and castrated rat pituitary. Endocrinology (1995) 0.97

Resistance of hypogonadic patients with mutated GnRH receptor genes to pulsatile GnRH administration. J Clin Endocrinol Metab (1999) 0.96

Pituitary adenylate cyclase-activating polypeptide and cyclic adenosine 3',5'-monophosphate stimulate the promoter activity of the rat gonadotropin-releasing hormone receptor gene via a bipartite response element in gonadotrope-derived cells. J Biol Chem (2001) 0.86

Fetal expression of GnRH and GnRH receptor genes in rat testis and ovary. J Endocrinol (1998) 0.86

The genes for gonadotropin-releasing hormone and its receptor are expressed in human breast with fibrocystic disease and cancer. Int J Cancer (1997) 0.85

Proximal cis-acting elements, including steroidogenic factor 1, mediate the efficiency of a distal enhancer in the promoter of the rat gonadotropin-releasing hormone receptor gene. Mol Endocrinol (2001) 0.84

Cyclic adenosine monophosphate and phorbol ester, like gonadotropin-releasing hormone, stimulate the biosynthesis of luteinizing hormone polypeptide chains in a nonadditive manner. Mol Endocrinol (1989) 0.84

Gonadotropin-releasing hormone and pituitary adenylate cyclase-activating polypeptide affect levels of cyclic adenosine 3',5'-monophosphate-dependent protein kinase A (PKA) subunits in the clonal gonadotrope alphaT3-1 cells: evidence for cross-talk between PKA and protein kinase C pathways. Endocrinology (1997) 0.84

A new compound heterozygous mutation of the gonadotropin-releasing hormone receptor (L314X, Q106R) in a woman with complete hypogonadotropic hypogonadism: chronic estrogen administration amplifies the gonadotropin defect. J Clin Endocrinol Metab (2000) 0.82

Temperature dependence of adenylate cyclase activity from rat white adipocytes. Mol Cell Endocrinol (1977) 0.81

[3H]-Thyroliberin (TRH) binding to nuclei isolated from a pituitary clonal cell line (GH3). Neuroendocrinology (1977) 0.80

Evidence that gonadotropin-releasing hormone stimulates gene expression and levels of active nitric oxide synthase type I in pituitary gonadotrophs, a process altered by desensitization and, indirectly, by gonadal steroids. Endocrinology (1998) 0.80

Effect of gonadectomy on pituitary levels of mRNA encoding gonadotropin subunits and secretion of luteinizing hormone. Mol Cell Endocrinol (1984) 0.79

Cell-free translation of the rat pituitary messenger RNA coding for the precursors of alpha and beta subunits of lutropin. FEBS Lett (1981) 0.79

Changes in LHbeta-gene and FSHbeta-gene expression in the ram pars tuberalis according to season and castration. Cell Tissue Res (1995) 0.79

Localization of luteinizing hormone beta-mRNA by in situ hybridization in the sheep pars tuberalis. Cell Tissue Res (1992) 0.79

Synergistic effects of progesterone and oestradiol on rat LH subunit mRNA. J Mol Endocrinol (1990) 0.79

Tissue-specific pattern of variant transcripts of the human gonadotropin-releasing hormone receptor gene. Eur J Endocrinol (1999) 0.78

Estradiol negatively regulates secretogranin II and chromogranin A messenger ribonucleic acid levels in the female rat pituitary but not in the adrenal. Endocrinology (1991) 0.78

GnRH and PACAP action in gonadotropes: cross-talk between phosphoinositidase C and adenylyl cyclase mediated signaling pathways. Trends Endocrinol Metab (1996) 0.78

Nucleotide sequence analysis of mRNAs predicts that rat pituitary and gonadal gonadotropin-releasing hormone receptor proteins have identical primary structure. Biochem Biophys Res Commun (1994) 0.78

Norepinephrine control of endogenous polypeptide phosphorylation in white fat cells. FEBS Lett (1978) 0.78

Estradiol regulates mRNAs encoding precursors to rat lutropin (LH) and follitropin (FSH) subunits. Biochem Biophys Res Commun (1983) 0.78

LH down-regulates gonadotropin-releasing hormone (GnRH) receptor, but not GnRH, mRNA levels in the rat testis. J Endocrinol (1999) 0.78

Functional importance of transmembrane helix 6 Trp(279) and exoloop 3 Val(299) of rat gonadotropin-releasing hormone receptor. Mol Pharmacol (2000) 0.78

Cloning and sequence analysis of the cDNA for the precursor of the beta subunit of ovine luteinizing hormone. Nucleic Acids Res (1990) 0.78

Isolation and characterization of a rat nitric oxide synthase type I gene promoter that confers expression and regulation in pituitary gonadotrope cells. Endocrinology (2001) 0.77

Multiple elements in the distal part of the 1.2 kb 5'-flanking region of the rat GnRH receptor gene regulate gonadotrope-specific expression conferred by proximal domain. Mol Cell Endocrinol (1998) 0.77

[Ambivalent effect of thyroxine on the expression of gonadotropin genes in normal and orchidectomized rats]. Pathol Biol (Paris) (1988) 0.77

Gonadotropin-releasing hormone stimulates the synthesis of the polypeptide chains of luteinizing hormone. Endocrinology (1986) 0.77

GnRH-dependent up-regulation of nitric oxide synthase I level in pituitary gonadotrophs mediates cGMP elevation during rat proestrus. Mol Cell Endocrinol (1998) 0.77

Cloning and sequence analysis of the cDNA for the pituitary glycoprotein hormone alpha-subunit of the European eel. Mol Cell Endocrinol (1990) 0.77

Differential stability of mRNAs coding for alpha and gonadotropin beta subunits in cultured rat pituitary cells. Mol Cell Endocrinol (1992) 0.76

Rat gonadotropin-releasing hormone receptor expressed in insect cells induces activation of adenylyl cyclase. Mol Cell Endocrinol (1997) 0.76

Sex steroid-binding protein: identification and comparison of the primary product following cell-free translation of human and monkey (Macaca fascicularis) liver RNA. J Steroid Biochem (1989) 0.76

Effect of testosterone on regulation of the level of sex steroid-binding protein mRNA in monkey (Macaca fascicularis) liver. J Mol Endocrinol (1990) 0.76

[Antilipolytic role of tetracycline. Inhibition of adenylate cyclase in vitro]. Eur J Biochem (1973) 0.76

Modulation of regulatory and catalytic subunit levels of cAMP-dependent protein kinase A in anterior pituitary cells in response to direct activation of protein kinases A and C or after GnRH stimulation. Neuroendocrinology (1995) 0.76

Testosterone inhibits the basal and gonadotropin-releasing hormone-stimulated synthesis and release of newly synthesized alpha- and lutropin (LH) beta-subunit but not release of stored LH in cultured rat pituitary cells. Biochim Biophys Acta (1996) 0.76

Mutations of the GnRH receptor gene: a new cause of autosomal-recessive hypogonadotropic hypogonadism. Arch Med Res (2000) 0.76

Subregional mapping of the human gonadotropin-releasing hormone receptor (GnRH-R) gene to 4q between the markers D4S392 and D4S409. Hum Genet (1995) 0.76

Critical implication of transmembrane Phe310, possibly in conjunction with Trp279, in the rat gonadotropin-releasing hormone receptor activation. Biochem Pharmacol (2001) 0.76

Molecular cloning and sequence analysis of the cDNA for the putative beta subunit of the type-II gonadotrophin from the European eel. J Mol Endocrinol (1990) 0.75

[Cyclic AMP: role in the synthesis and release of luteinizing hormone]. Pathol Biol (Paris) (1990) 0.75

Detection of HIV-1 infections by PCR: evaluation in a seropositive subject population. Mol Cell Probes (1992) 0.75

[Hormonal regulation of the expression of genes coding for pituitary gonadotropins]. Ann Endocrinol (Paris) (1986) 0.75

[Stimulation of LH gene expression by GnRH. Role of protein kinases A and C]. Pathol Biol (Paris) (1989) 0.75

Molecular mechanisms involved in the hormonal regulation of prolactin and gonadotropins biosynthesis in the pituitary gland. Endokrynol Pol (1985) 0.75

Insect cells infected with a recombinant baculovirus express both O- and N-glycosylated forms of the rat glycoprotein hormone alpha-subunit. J Mol Endocrinol (1996) 0.75

[Relative non-masking of noradrenaline receptors by linoleic acid-rich phospholipids in fat cell membranes. I. Lipase activity]. Biochim Biophys Acta (1972) 0.75

The effect of dietary protein restriction on the secretion of LH and FSH in pre-pubertal female lambs. Anim Reprod Sci (2003) 0.75

[Focus on the biogenesis of hypophyseal gonadotropins]. J Steroid Biochem (1989) 0.75

[Possible unmasking of noradrenaline receptors by linoleic acid-rich phospholipids in fat cell membranes. II. Adenylate cyclase and phosphodiesterase activities]. Biochim Biophys Acta (1973) 0.75

Messenger ribonucleic acids for alpha- and beta-isoforms of cyclic adenosine 3',5'-monophosphate-dependent protein kinase subunits present in the anterior pituitary: regulation of RII beta and C alpha gene expression by the cyclic nucleotide and phorbol ester. Endocrinology (1993) 0.75

Characterization of the precursors of alpha and beta subunits of follitropin following cell-free translation of rat and ovine pituitary mRNAs. Biochem Biophys Res Commun (1982) 0.75

[Role of gonadal steroids in the regulation of messengers coding for pituitary gonadotropins]. Pathol Biol (Paris) (1984) 0.75

Several intermediate forms in the processing of rat lutropin subunits as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis. J Chromatogr (1988) 0.75

Glycosylation process in gonadotrophs: a quantitative electron microscope autoradiographic study with labeled glucosamine. Biol Cell (1987) 0.75

Synthesis of gonadotrophins and its regulation in the pituitary gland. Hum Reprod (1988) 0.75

Modulation of luteinizing hormone subunit gene expression by intracerebroventricular microinjection of gonadotropin-releasing hormone or beta-endorphin in female rats. Biochim Biophys Acta (2000) 0.75

Rat placental mRNA directs the synthesis of a polypeptide immunologically related to alpha-subunit of glycoprotein hormones. Mol Cell Endocrinol (1987) 0.75

Detection of a lag phase in gonadotropin-releasing hormone stimulated synthesis of lutropin peptide chains in cultured rat anterior pituitary cells. Biochem Biophys Res Commun (1988) 0.75

Biosynthesis of gonadotropin releasing hormone (GnRH): present status. Psychoneuroendocrinology (1983) 0.75

International symposium on G protein-coupled receptors. Biol Cell (2004) 0.75

[Adenylcyclase activity during fetal and neonatal development of brown adipose tissue. Drawback and criticism of measurement methods]. Bull Soc Chim Biol (Paris) (1970) 0.75

Identification of the primary translation product of the sex steroid-binding protein from monkey liver mRNA in a cell-free system. Biochimie (1988) 0.75

Estradiol has inverse effects on pituitary glycoprotein hormone alpha-subunit messenger ribonucleic acid in the immature European eel and the gonadectomized rat. Endocrinology (1987) 0.75

The GnRH receptor gene is preferentially expressed in functioning gonadotroph adenomas and displays a Mae III polymorphism site. Clin Endocrinol (Oxf) (1998) 0.75

Cyclic AMP enhances gene expression, synthesis and release of newly synthesized alpha and luteinizing hormone beta subunits in cultured rat anterior pituitary cells. Neurochem Int (1989) 0.75

[Adenylate cyclase/Lipase. Hormone receptor induction]. Biochimie (1976) 0.75

[A micromethod allowing the preparation and translation of messenger ribonucleic acid from adenohypophysis cells in culture]. C R Seances Acad Sci III (1981) 0.75

[Sexual cycle period and resistance of mice to acute experimental hypoxia]. C R Seances Soc Biol Fil (1977) 0.75

[Fetal and neonatal adenylcyclase of adipose tissue. Relation to the essential fatty acid proportion of exogenous lipids]. J Physiol (Paris) (1971) 0.75