Published in Methods Enzymol on January 01, 1983
A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. Mol Cell Biol (1987) 5.66
Defining the sequence specificity of DNA-binding proteins by selecting binding sites from random-sequence oligonucleotides: analysis of yeast GCN4 protein. Mol Cell Biol (1989) 5.23
GCN4 protein, a positive transcription factor in yeast, binds general control promoters at all 5' TGACTC 3' sequences. Proc Natl Acad Sci U S A (1986) 3.68
Expression and characterization of the human c-myc DNA-binding protein. Mol Cell Biol (1985) 3.24
Yeast TATA-box transcription factor gene. Proc Natl Acad Sci U S A (1989) 3.03
Translational efficiency of the Escherichia coli adenylate cyclase gene: mutating the UUG initiation codon to GUG or AUG results in increased gene expression. Proc Natl Acad Sci U S A (1985) 2.86
In vitro transcriptional activation, dimerization, and DNA-binding specificity of the Epstein-Barr virus Zta protein. J Virol (1990) 2.84
Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho. Proc Natl Acad Sci U S A (1985) 2.70
Nucleotide sequence and expression of the gene encoding the EcoRII modification enzyme. Nucleic Acids Res (1987) 2.68
Structure of two pectate lyase genes from Erwinia chrysanthemi EC16 and their high-level expression in Escherichia coli. J Bacteriol (1986) 2.30
Pentapeptide nuclear localization signal in adenovirus E1a. Mol Cell Biol (1987) 2.30
Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli. Proc Natl Acad Sci U S A (1983) 2.19
Intron mobility in phage T4 is dependent upon a distinctive class of endonucleases and independent of DNA sequences encoding the intron core: mechanistic and evolutionary implications. Nucleic Acids Res (1990) 2.19
Effects of a minor isoleucyl tRNA on heterologous protein translation in Escherichia coli. J Bacteriol (1995) 2.14
Functions of purified E1A protein microinjected into mammalian cells. Proc Natl Acad Sci U S A (1984) 1.92
Synthesis of the complete trans-activation gene product of human T-lymphotropic virus type III in Escherichia coli: demonstration of immunogenicity in vivo and expression in vitro. Proc Natl Acad Sci U S A (1986) 1.90
Purification and characterization of human H-ras proteins expressed in Escherichia coli. Mol Cell Biol (1985) 1.77
Genetic selection for genes encoding sequence-specific DNA-binding proteins. Proc Natl Acad Sci U S A (1989) 1.75
Nucleotide sequence of the argR gene of Escherichia coli K-12 and isolation of its product, the arginine repressor. Proc Natl Acad Sci U S A (1987) 1.67
Interpathway regulation of the TRP4 gene of yeast. EMBO J (1989) 1.65
Cloning, expression in Escherichia coli, and reconstitution of human myoglobin. Proc Natl Acad Sci U S A (1985) 1.59
Expression of influenza virus NS2 nonstructural protein in bacteria and localization of NS2 in infected eucaryotic cells. J Virol (1985) 1.55
Mapping of functional domains in adenovirus E1A proteins. Proc Natl Acad Sci U S A (1985) 1.53
Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res. Proc Natl Acad Sci U S A (1986) 1.52
Transcription mapping of the Escherichia coli chromosome by electron microscopy. J Bacteriol (1989) 1.50
Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products. Nucleic Acids Res (1989) 1.46
DNA and amino acid sequence analysis of structural and immunity genes of colicins Ia and Ib. J Bacteriol (1986) 1.45
The precursor of beta-lactamase: purification, properties and folding kinetics. EMBO J (1989) 1.37
Influenza virus hemagglutinin-specific cytotoxic T cell response induced by polypeptide produced in Escherichia coli. J Exp Med (1985) 1.37
Expression of the art gene protein of human T-lymphotropic virus type III (HTLV-III/LAV) in bacteria. J Virol (1987) 1.28
Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Pseudomonas putida. J Bacteriol (1990) 1.23
Cloning, nucleotide sequence, and overexpression of the bacteriophage T4 regA gene. Proc Natl Acad Sci U S A (1985) 1.19
Characterization of the restriction site of a prokaryotic intron-encoded endonuclease. Proc Natl Acad Sci U S A (1990) 1.17
Bacteriophage T4 regA protein binds to the Shine-Dalgarno region of gene 44 mRNA. Nucleic Acids Res (1989) 1.16
Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase. J Bacteriol (1986) 1.15
Transcriptional regulatory elements stimulate recombination in extrachromosomal substrates carrying immunoglobulin switch-region sequences. Proc Natl Acad Sci U S A (1992) 1.12
Fabry disease: isolation of a cDNA clone encoding human alpha-galactosidase A. Proc Natl Acad Sci U S A (1985) 1.11
Protein-protein interactions directing resolvase site-specific recombination: a structure-function analysis. EMBO J (1993) 1.10
Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway. Proc Natl Acad Sci U S A (1986) 1.09
Characterisation of the DNA-binding profile of barley HvCBF1 using an enzymatic method for rapid, quantitative and high-throughput analysis of the DNA-binding activity. Nucleic Acids Res (2002) 1.05
Efficient expression of the yeast metallothionein gene in Escherichia coli. J Bacteriol (1988) 1.05
Immunochemical dissection of the Ultrabithorax homeoprotein family in Drosophila melanogaster. Proc Natl Acad Sci U S A (1991) 1.04
Bacteriophage P2 ogr and P4 delta genes act independently and are essential for P4 multiplication. J Bacteriol (1990) 1.04
Nucleotide sequence and protein overproduction of bacteriophage T4 thioredoxin. J Virol (1986) 1.02
Intron homing with limited exon homology. Illegitimate double-strand-break repair in intron acquisition by phage t4. Genetics (1999) 0.96
Initiation of plasmid R1 replication in vitro is independent of transcription by host RNA polymerase. Nucleic Acids Res (1984) 0.96
Self-assembly of proglycinin and hybrid proglycinin synthesized in vitro from cDNA. Proc Natl Acad Sci U S A (1987) 0.94
Cloning of T4 gene 32 and expression of the wild-type protein under lambda promoter PL regulation in Escherichia coli. Proc Natl Acad Sci U S A (1986) 0.93
Aggregatibacter actinomycetemcomitans leukotoxin is post-translationally modified by addition of either saturated or hydroxylated fatty acyl chains. Mol Oral Microbiol (2011) 0.91
Blocking of passive sensitization of human mast cells and basophil granulocytes with IgE antibodies by a recombinant human epsilon-chain fragment of 76 amino acids. Proc Natl Acad Sci U S A (1989) 0.90
Processing of Escherichia coli 16S rRNA with bacteriophage lambda leader sequences. J Bacteriol (1987) 0.90
Expression of the cloned gene for enterotoxin STb of Escherichia coli. Infect Immun (1990) 0.90
The control of lambda DNA terminase synthesis. Nucleic Acids Res (1987) 0.88
Secretion into the culture medium of a foreign gene product from Escherichia coli: use of the ompF gene for secretion of human beta-endorphin. EMBO J (1985) 0.87
Inhibitory effect of high-level transcription of the bacteriophage lambda nutL region on transcription of rRNA in Escherichia coli. J Bacteriol (1985) 0.87
F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems. J Bacteriol (1985) 0.86
Adenovirus type 12 E1A protein expressed in Escherichia coli is functional upon transfer by microinjection or protoplast fusion into mammalian cells. J Virol (1986) 0.85
Selection for mutations in the PR promoter of bacteriophage lambda. Nucleic Acids Res (1990) 0.79
RegA proteins from phage T4 and RB69 have conserved helix-loop groove RNA binding motifs but different RNA binding specificities. Nucleic Acids Res (2001) 0.77
Improved genetically modified Escherichia coli strain for prescreening antineoplastic agents. Antimicrob Agents Chemother (1984) 0.75
Re-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategies. Nucleic Acids Res (1993) 0.75
Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet (1979) 54.83
Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells. Cell (1982) 9.27
A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif Anal (1981) 8.21
Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science (2001) 5.40
The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda. Nature (1978) 5.38
Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. Nat Genet (1995) 5.02
Crystal structure of an HIV-binding recombinant fragment of human CD4. Nature (1990) 4.81
The product of ras is a GTPase and the T24 oncogenic mutant is deficient in this activity. Nature (1984) 4.61
Purified lambda regulatory protein cII positively activates promoters for lysogenic development. Nature (1981) 4.60
Termination of transcription in E. coli. Cell (1983) 4.42
Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc Natl Acad Sci U S A (1982) 4.25
Ferritin: a cytoprotective antioxidant strategem of endothelium. J Biol Chem (1992) 4.22
Clinical and immunologic criteria for the diagnosis of allergic bronchopulmonary aspergillosis. Ann Intern Med (1977) 4.14
Efficient translation of prokaryotic mRNAs in a eukaryotic cell-free system requires addition of a cap structure. Nature (1979) 4.11
Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL. J Biol Chem (1998) 3.88
Microinjection of the oncogene form of the human H-ras (T-24) protein results in rapid proliferation of quiescent cells. Cell (1984) 3.81
Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor. Proc Natl Acad Sci U S A (1987) 3.72
Termination of transcription in bacteriophage lambda. Heterogeneous, 3'-terminal oligo-adenylate additions and the effects of rho factor. J Biol Chem (1975) 3.71
Surveillance and prevention of residential-fire injuries. N Engl J Med (1996) 3.60
Translational coupling at an intercistronic boundary of the Escherichia coli galactose operon. Cell (1982) 3.56
Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice. Proc Natl Acad Sci U S A (1989) 3.55
A genomic analysis of two-component signal transduction in Streptococcus pneumoniae. Mol Microbiol (2000) 3.50
Highly reiterated sequences of SIMIANSIMIANSIMIANSIMIANSIMIAN. Science (1978) 3.29
Expression and characterization of the human c-myc DNA-binding protein. Mol Cell Biol (1985) 3.24
Constitutive function of a positively regulated promoter reveals new sequences essential for activity. J Biol Chem (1987) 3.23
Role of adherence in growth of Acinetobacter calcoaceticus RAG-1 on hexadecane. J Bacteriol (1981) 2.96
gridlock, an HLH gene required for assembly of the aorta in zebrafish. Science (2000) 2.90
Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles. Proc Natl Acad Sci U S A (1978) 2.82
Sequence of Cro gene of bacteriophage lambda. Nature (1977) 2.78
Stability of curcumin in buffer solutions and characterization of its degradation products. J Pharm Biomed Anal (1997) 2.77
Copper metallothionein of yeast, structure of the gene, and regulation of expression. Proc Natl Acad Sci U S A (1984) 2.74
Adherence of Acinetobacter calcoaceticus RAG-1 to human epithelial cells and to hexadecane. Infect Immun (1981) 2.74
Bacterial adherence to polystyrene: a replica method of screening for bacterial hydrophobicity. Appl Environ Microbiol (1981) 2.73
Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho. Proc Natl Acad Sci U S A (1985) 2.70
E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA. Mol Cell Biol (1985) 2.68
Endocytosis in Chang liver cells. Quantitation by sucrose- 3 H uptake and inhibition by cytochalasin B. J Cell Biol (1971) 2.63
A rho-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli. Nature (1978) 2.58
Affecting gene expression by altering the length and sequence of the 5' leader. Proc Natl Acad Sci U S A (1984) 2.58
Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli. Proc Natl Acad Sci U S A (1977) 2.57
Microinjected c-myc as a competence factor. Science (1985) 2.56
Evolution of two-component signal transduction. Mol Biol Evol (2000) 2.55
Envelope glycoproteins from biologically diverse isolates of immunodeficiency viruses have widely different affinities for CD4. Proc Natl Acad Sci U S A (1991) 2.54
Identification, evolution, and essentiality of the mevalonate pathway for isopentenyl diphosphate biosynthesis in gram-positive cocci. J Bacteriol (2000) 2.50
Overexpression of Bcl-2 attenuates apoptosis and protects against myocardial I/R injury in transgenic mice. Am J Physiol Heart Circ Physiol (2001) 2.38
Adenovirus E1a gene product expressed at high levels in Escherichia coli is functional. Science (1984) 2.38
Structure and function of the cy control region of bacteriophage lambda. J Mol Biol (1980) 2.37
A promoter of pBR322 activated by cAMP receptor protein. Nucleic Acids Res (1981) 2.37
Pentapeptide nuclear localization signal in adenovirus E1a. Mol Cell Biol (1987) 2.30
The influence of age, diagnosis, and gender on proper use of metered-dose inhalers. Am J Respir Crit Care Med (1994) 2.23
Promoter for the establishment of repressor synthesis in bacteriophage lambda. Proc Natl Acad Sci U S A (1980) 2.23
Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli. Proc Natl Acad Sci U S A (1983) 2.19
Red cells from glutathione peroxidase-1-deficient mice have nearly normal defenses against exogenous peroxides. Blood (2000) 2.19
Removal of a terminator structure by RNA processing regulates int gene expression. J Mol Biol (1984) 2.13
Bacteriophage lambda protein cII binds promoters on the opposite face of the DNA helix from RNA polymerase. Nature (1983) 2.13
Nucleotide sequences of the 5' and 3' termini of bacteriophage T7 early messenger RNAs synthesized in vivo: evidence for sequence specificity in RNA processing. J Mol Biol (1974) 2.10
The protective role of manganese superoxide dismutase against adriamycin-induced acute cardiac toxicity in transgenic mice. J Clin Invest (1996) 2.07
Interaction between FKBP12-rapamycin and TOR involves a conserved serine residue. J Biol Chem (1994) 2.04
The effects of a 5-lipoxygenase inhibitor on asthma induced by cold, dry air. N Engl J Med (1990) 2.03
Isolation of type 5 adenovirus mutants with a cold-sensitive host range phenotype: genetic evidence of an adenovirus transformation maintenance function. Virology (1982) 1.99
The expression in yeast of the Escherichia coli galK gene on CYC1::galK fusion plasmids. Gene (1983) 1.98
Characterization of short tandem repeats from thirty-one human telomeres. Genome Res (1997) 1.96
Functions of purified E1A protein microinjected into mammalian cells. Proc Natl Acad Sci U S A (1984) 1.92
Synthesis of the complete trans-activation gene product of human T-lymphotropic virus type III in Escherichia coli: demonstration of immunogenicity in vivo and expression in vitro. Proc Natl Acad Sci U S A (1986) 1.90
Regulated antisense RNA eliminates alpha-toxin virulence in Staphylococcus aureus infection. J Bacteriol (1999) 1.88
Differential translation efficiency explains discoordinate expression of the galactose operon. Cell (1981) 1.87
Microarray-based identification of htrA, a Streptococcus pneumoniae gene that is regulated by the CiaRH two-component system and contributes to nasopharyngeal colonization. Infect Immun (2002) 1.87
Activation of human immunodeficiency virus type 1 by DNA damage in human cells. Nature (1988) 1.85
The 289-amino acid E1A protein of adenovirus binds zinc in a region that is important for trans-activation. Proc Natl Acad Sci U S A (1988) 1.84
Two promoters, one inducible and one constitutive, control transcription of the Streptomyces lividans galactose operon. Proc Natl Acad Sci U S A (1987) 1.79
Purification and characterization of human H-ras proteins expressed in Escherichia coli. Mol Cell Biol (1985) 1.77
Impaired nuclear transport of a human variant tRNAiMet. Nature (1982) 1.75
Overexpression of MnSOD protects against myocardial ischemia/reperfusion injury in transgenic mice. J Mol Cell Cardiol (1998) 1.72
Inhibition of bacterial adherence to hydrocarbons and epithelial cells by emulsan. Infect Immun (1983) 1.71
Trimellitic anhydride-induced airway syndromes: clinical and immunologic studies. J Allergy Clin Immunol (1977) 1.71
Microbicides--evaluating multiple formulations of C31G. Contraception (2002) 1.67
Regulation of sodium-calcium exchange and mitochondrial energetics by Bcl-2 in the heart of transgenic mice. J Mol Cell Cardiol (2001) 1.65
Studying promoters and terminators by gene fusion. Science (1983) 1.64
Serum IgE and IgG antibody activity against Aspergillus fumigatus as a diagnostic aid in allergic bronchopulmonary aspergillosis. Am Rev Respir Dis (1978) 1.62
Envelope proteins from clinical isolates of human immunodeficiency virus type 1 that are refractory to neutralization by soluble CD4 possess high affinity for the CD4 receptor. Proc Natl Acad Sci U S A (1991) 1.62
Determination of nucleotide sequences beyond the sites of transcriptional termination. Proc Natl Acad Sci U S A (1976) 1.61
Human immunodeficiency virus 1 protease expressed in Escherichia coli behaves as a dimeric aspartic protease. Proc Natl Acad Sci U S A (1989) 1.60
A physical map of the human Y chromosome. Nature (2001) 1.58
T lymphocyte lines producing or vaccinating against autoimmune encephalomyelitis (EAE). Functional activation induces peanut agglutinin receptors and accumulation in the brain and thymus of line cells. Eur J Immunol (1983) 1.58
Regulated expression at high copy number allows production of a growth-inhibitory oncogene product in Drosophila Schneider cells. Genes Dev (1989) 1.56
Regulated gene expression in Staphylococcus aureus for identifying conditional lethal phenotypes and antibiotic mode of action. Gene (2000) 1.55
Characterization of Streptomyces promoter sequences using the Escherichia coli galactokinase gene. Gene (1985) 1.55
The srhSR gene pair from Staphylococcus aureus: genomic and proteomic approaches to the identification and characterization of gene function. Biochemistry (2001) 1.54
Isolation and sequence determination of the 3'-terminal regions of isotopically labelled RNA molecules. Nucleic Acids Res (1974) 1.53
Structure of simian virus 40 recombinants that contain both host and viral DNA sequences. I. The structure of variant CVPS/1/P2 (EcoRI res). J Biol Chem (1979) 1.53
Mitochondrial superoxide production in kainate-induced hippocampal damage. Neuroscience (2000) 1.53
Mapping of functional domains in adenovirus E1A proteins. Proc Natl Acad Sci U S A (1985) 1.53
Control of transcription termination: a rho-dependent termination site in bacteriophage lambda. J Mol Biol (1980) 1.51
Studies on the interactions of nucleotides, polynucleotides, and nucleic acids with dihydroxyboryl-substituted celluloses. Biochemistry (1972) 1.51
Nucleotide sequence surrounding a ribonuclease III processing site in bacteriophage T7 RNA. Proc Natl Acad Sci U S A (1977) 1.50
Structure of simian virus 40 recombinants that contain both host and viral DNA sequences. II. The structure of variant 1103 and its comparison to variant CVPS/1P2 (EcoRI res). J Biol Chem (1979) 1.49
Results of a gender-climate and work-environment survey at a midwestern academic health center. Acad Med (2000) 1.48
Differential activation of the mouse beta-globin promoter by enhancers. Mol Cell Biol (1983) 1.47
Left atrial ball thrombus in nonrheumatic atrial fibrillation diagnosed by transesophageal echocardiography. Am Heart J (1992) 1.46
From the Surgeon General, US Public Health Service. JAMA (1992) 1.45