Published in Methods Enzymol on January 01, 1983
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics (1989) 105.30
Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiol Mol Biol Rev (1999) 19.25
Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast. Proc Natl Acad Sci U S A (1985) 13.43
Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes. Mol Cell Biol (1998) 10.34
Isolation, characterization, and inactivation of the APA1 gene encoding yeast diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase. J Bacteriol (1989) 9.34
Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev (2002) 8.50
Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein. Mol Cell Biol (1987) 7.38
Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae. Mol Cell Biol (1996) 7.33
The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. Mol Cell Biol (1994) 7.31
The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). EMBO J (1996) 6.66
Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J Cell Biol (1992) 6.37
The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog. EMBO J (1988) 6.09
Disruption of mitotic spindle orientation in a yeast dynein mutant. Proc Natl Acad Sci U S A (1993) 5.94
Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol Cell Biol (1987) 5.84
Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1986) 5.75
Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A (1986) 5.71
Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information. Mol Cell Biol (1988) 5.42
hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures. Mol Cell Biol (1989) 5.37
Protein interaction cloning in yeast: identification of mammalian proteins that react with the leucine zipper of Jun. Proc Natl Acad Sci U S A (1992) 5.28
Kinesin-related proteins required for assembly of the mitotic spindle. J Cell Biol (1992) 5.26
Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae. Mol Cell Biol (1990) 5.25
Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins. EMBO J (1990) 5.10
A family of cyclin homologs that control the G1 phase in yeast. Proc Natl Acad Sci U S A (1989) 5.09
DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae. Mol Cell Biol (1988) 5.08
Four mating-type genes control sexual differentiation in the fission yeast. EMBO J (1988) 5.00
Two alternative pathways of double-strand break repair that are kinetically separable and independently modulated. Mol Cell Biol (1992) 4.97
NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae. J Cell Biol (1993) 4.96
A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability. EMBO J (1989) 4.91
High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc Natl Acad Sci U S A (2010) 4.78
Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant. Mol Biol Cell (1996) 4.71
Genome engineering with zinc-finger nucleases. Genetics (2011) 4.66
Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics (1990) 4.63
SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae. Genetics (1996) 4.62
The recognition component of the N-end rule pathway. EMBO J (1990) 4.55
Cloning and characterization of four SIR genes of Saccharomyces cerevisiae. Mol Cell Biol (1986) 4.53
Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication. Proc Natl Acad Sci U S A (1996) 4.49
Instability of simple sequence DNA in Saccharomyces cerevisiae. Mol Cell Biol (1992) 4.46
The product of the mei3+ gene, expressed under control of the mating-type locus, induces meiosis and sporulation in fission yeast. EMBO J (1987) 4.45
Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes from Saccharomyces cerevisiae. EMBO J (1985) 4.37
A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site. EMBO J (1988) 4.34
Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Mol Cell Biol (1986) 4.33
Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae. Genetics (1996) 4.33
The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenance. Mol Cell Biol (1999) 4.32
GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway. Mol Cell Biol (1994) 4.30
Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J (1993) 4.27
Evidence that the MIF2 gene of Saccharomyces cerevisiae encodes a centromere protein with homology to the mammalian centromere protein CENP-C. Mol Biol Cell (1995) 4.23
PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors. Mol Cell Biol (1986) 4.23
Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment. J Cell Biol (1992) 4.20
Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae. Mol Cell Biol (1987) 4.17
Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J (1993) 4.10
Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells. EMBO J (1989) 4.10
Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol (1998) 4.08
Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast. EMBO J (1989) 4.08
The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal. J Cell Biol (1986) 4.07
Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a factor: gene sequence and implications for the structure of the presumed receptor. Proc Natl Acad Sci U S A (1986) 4.07
Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins. Mol Cell Biol (1992) 4.04
Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol (1993) 4.03
Dominant mutations in a gene encoding a putative protein kinase (BCK1) bypass the requirement for a Saccharomyces cerevisiae protein kinase C homolog. Mol Cell Biol (1992) 4.02
Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences. Mol Cell Biol (1988) 4.02
The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene. Genetics (1989) 4.00
Physical mapping of large DNA by chromosome fragmentation. Proc Natl Acad Sci U S A (1988) 3.95
A low copy number central sequence with strict symmetry and unusual chromatin structure in fission yeast centromere. Mol Biol Cell (1992) 3.94
The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. EMBO J (1992) 3.91
Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Mol Cell Biol (1999) 3.90
Identification of silencer binding proteins from yeast: possible roles in SIR control and DNA replication. EMBO J (1987) 3.86
Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y. J Cell Biol (1986) 3.85
Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. EMBO J (1994) 3.83
Effect of the laccase gene CNLAC1, on virulence of Cryptococcus neoformans. J Exp Med (1996) 3.76
Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae. Mol Cell Biol (1987) 3.75
Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product. Mol Cell Biol (1988) 3.74
The yeast homologue of U3 snRNA. EMBO J (1987) 3.74
GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast. EMBO J (1992) 3.72
Double targeted gene replacement for creating null mutants. Proc Natl Acad Sci U S A (1991) 3.72
A spacer protein in the Saccharomyces cerevisiae spindle poly body whose transcript is cell cycle-regulated. J Cell Biol (1993) 3.71
Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 3.70
Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species. Proc Natl Acad Sci U S A (1989) 3.70
Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily. Mol Cell Biol (1991) 3.68
CPF1, a yeast protein which functions in centromeres and promoters. EMBO J (1990) 3.65
Nuclease hypersensitive regions with adjacent positioned nucleosomes mark the gene boundaries of the PHO5/PHO3 locus in yeast. EMBO J (1986) 3.63
The a-factor pheromone of Saccharomyces cerevisiae is essential for mating. Mol Cell Biol (1988) 3.62
Meiosis in asynaptic yeast. Genetics (1990) 3.60
Molecular analysis of SSN6, a gene functionally related to the SNF1 protein kinase of Saccharomyces cerevisiae. Mol Cell Biol (1987) 3.57
A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1. EMBO J (1992) 3.57
Pathway correcting DNA replication errors in Saccharomyces cerevisiae. EMBO J (1993) 3.57
The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex. J Cell Biol (1989) 3.55
MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C. Mol Cell Biol (1993) 3.54
Expansions and contractions of the genetic map relative to the physical map of yeast chromosome III. Mol Cell Biol (1988) 3.53
The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases. Mol Cell Biol (1991) 3.52
HOP1: a yeast meiotic pairing gene. Genetics (1989) 3.51
Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1987) 3.50
Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease. Proc Natl Acad Sci U S A (1989) 3.49
Essential functional interactions of SAGA, a Saccharomyces cerevisiae complex of Spt, Ada, and Gcn5 proteins, with the Snf/Swi and Srb/mediator complexes. Genetics (1997) 3.45
Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity. Proc Natl Acad Sci U S A (1991) 3.44
Effect of mutations in genes affecting homologous recombination on restriction enzyme-mediated and illegitimate recombination in Saccharomyces cerevisiae. Mol Cell Biol (1994) 3.41
Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein. Mol Cell Biol (1993) 3.39
Characterization of RAD9 of Saccharomyces cerevisiae and evidence that its function acts posttranslationally in cell cycle arrest after DNA damage. Mol Cell Biol (1990) 3.38
Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4. Genes Dev (1999) 3.37
A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria. Proc Natl Acad Sci U S A (1991) 3.37
A unique pathway of double-strand break repair operates in tandemly repeated genes. Mol Cell Biol (1991) 3.36
The yeast Ca(2+)-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. Mol Biol Cell (1992) 3.35
The double-strand-break repair model for recombination. Cell (1983) 31.21
Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci U S A (1981) 26.54
Genetic applications of yeast transformation with linear and gapped plasmids. Methods Enzymol (1983) 8.96
Genes affecting the expression of cytochrome c in yeast: genetic mapping and genetic interactions. Genetics (1980) 3.63
The effect of ochre suppression on meiosis and ascospore formation in Saccharomyces. Genetics (1977) 2.24
Synthetic adaptors for cloning DNA. Methods Enzymol (1979) 2.14
Electrocardiographic criteria for tricyclic antidepressant cardiotoxicity. Am J Cardiol (1986) 1.56
A genetic fine structure analysis of the suppressor 3 locus in Saccharomyces. Genetics (1977) 1.45
Assessment of suicide potential by nonpsychiatrists using the SAD PERSONS score. J Emerg Med (1988) 1.43
Anaphylactic reaction to zomepirac. Ann Allergy (1982) 1.39
Dependence on mating type for the overproduction of iso-2-cytochrome c in the yeast mutant CYC7-H2. Genetics (1980) 1.17
Colchicine overdose: report of two cases and review of the literature. Ann Emerg Med (1981) 1.15
Myocardial contusion. JAMA (1983) 1.07
Assessing severity of adult asthma and need for hospitalization. Ann Emerg Med (1985) 1.01
Varicella pneumonia in adults: a spectrum of disease. Ann Emerg Med (1986) 0.86
Tetanus. Prevention and treatment. JAMA (1978) 0.83
Myocardial contusion diagnosed by first-pass radionuclide angiography. Am J Emerg Med (1986) 0.83
Respiratory-related activity of upper airway muscles in anesthetized rabbit. J Appl Physiol Respir Environ Exerc Physiol (1983) 0.83
Evaluation of patients with chest pain and nondiagnostic ECG using thallium-201 myocardial planar imaging and technetium-99m first-pass radionuclide angiography in the emergency department. Ann Emerg Med (1992) 0.82
Condylar guidance settings on articulators from protrusive records. J Prosthet Dent (1972) 0.81
Appendicitis: evaluation by Tc-99m leukocyte scan. Ann Emerg Med (1988) 0.79
Genetics society of america program and abstracts. Genetics (1989) 0.77
Emergency medicine-important advances in clinical medicine: naloxone-new uses? West J Med (1983) 0.75
Effect of respiratory alkalosis in tricyclic antidepressant overdose. West J Med (1983) 0.75
Esophageal electrocardiography in acute cardiac care. Efficacy and diagnostic value of a new technique. Am J Med (1987) 0.75
Hemodynamic effects of pneumatic external counterpressure in canine hemorrhagic shock. Ann Emerg Med (1983) 0.75
Emergency management of poisoning and overdose. Compr Ther (1979) 0.75
Cardiac conduction and rhythm disturbances following suicidal ingestion of mesoridazine. Ann Emerg Med (1981) 0.75
Futility of skull radiography for nontraumatic conditions. Ann Emerg Med (1982) 0.75
Use of naloxone during cardiac arrest and CPR: potential adjunct for postcountershock electrical-mechanical dissociation. Ann Emerg Med (1985) 0.75
Syphilis and pelvic inflammatory disease. JACEP (1978) 0.75
Letter: Status asthmaticus. JAMA (1975) 0.75
Tetanus toxoid boosters. JAMA (1978) 0.75
Intravenous theophylline therapy in asthma: a clinical update. Ann Emerg Med (1980) 0.75
NMB usage survey. Ann Emerg Med (1986) 0.75
Nomograms for predicted FEV1 and FVC in children, adolescents, and adults. Mt Sinai J Med (1985) 0.75
Regarding the remote practice of allergy. J Allergy Clin Immunol (1988) 0.75
Pioneers in the dental laboratory field. Bull Hist Dent (1969) 0.75