In vitro assembly of homopolymer and copolymer filaments from intermediate filament subunits of muscle and fibroblastic cells.

PubWeight™: 2.02‹?› | Rank: Top 2%

🔗 View Article (PMC 319637)

Published in Proc Natl Acad Sci U S A on June 01, 1981

Authors

P M Steinert, W W Idler, F Cabral, M M Gottesman, R D Goldman

Articles citing this

Viscoelastic properties of vimentin compared with other filamentous biopolymer networks. J Cell Biol (1991) 3.86

Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. FASEB J (2010) 3.05

The expression of mutant epidermal keratin cDNAs transfected in simple epithelial and squamous cell carcinoma lines. J Cell Biol (1987) 2.61

Characterization of a class of cationic proteins that specifically interact with intermediate filaments. Proc Natl Acad Sci U S A (1981) 2.15

The role of keratin subfamilies and keratin pairs in the formation of human epidermal intermediate filaments. J Cell Biol (1986) 2.04

Posttranslational regulation of keratins: degradation of mouse and human keratins 18 and 8. Mol Cell Biol (1989) 2.00

Expression of mutant keratin cDNAs in epithelial cells reveals possible mechanisms for initiation and assembly of intermediate filaments. J Cell Biol (1989) 1.89

The function of intermediate filaments in cell shape and cytoskeletal integrity. J Cell Biol (1996) 1.82

Primary and secondary structure of hamster vimentin predicted from the nucleotide sequence. Proc Natl Acad Sci U S A (1983) 1.75

Heteropolymer filaments of vimentin and desmin in vascular smooth muscle tissue and cultured baby hamster kidney cells demonstrated by chemical crosslinking. Proc Natl Acad Sci U S A (1982) 1.68

Isolation and characterization of keratin-like proteins from cultured cells with fibroblastic morphology. J Cell Biol (1984) 1.40

Structure of fibroblastic intermediate filaments: analysis of scanning transmission electron microscopy. Proc Natl Acad Sci U S A (1982) 1.36

A 300,000-mol-wt intermediate filament-associated protein in baby hamster kidney (BHK-21) cells. J Cell Biol (1985) 1.34

Vimentin filaments are assembled from a soluble precursor in avian erythroid cells. J Cell Biol (1983) 1.34

Proteolysis of vimentin and desmin by the Ca2+-activated proteinase specific for these intermediate filament proteins. Mol Cell Biol (1983) 1.31

The binding of vimentin to human erythrocyte membranes: a model system for the study of intermediate filament-membrane interactions. J Cell Biol (1985) 1.27

Amino acid sequence data on glial fibrillary acidic protein (GFA); implications for the subdivision of intermediate filaments into epithelial and non-epithelial members. EMBO J (1983) 1.24

Epidermal keratin filaments assembled in vitro have masses-per-unit-length that scale according to average subunit mass: structural basis for homologous packing of subunits in intermediate filaments. J Cell Biol (1983) 1.17

Intermediate filaments. J Cell Biol (1984) 1.14

Identification of glial filament protein and vimentin in the same intermediate filament system in human glioma cells. Proc Natl Acad Sci U S A (1984) 1.14

Expression of NF-L and NF-M in fibroblasts reveals coassembly of neurofilament and vimentin subunits. J Cell Biol (1989) 1.10

Cyclic AMP-modulated phosphorylation of intermediate filament proteins in cultured avian myogenic cells. Mol Cell Biol (1982) 1.08

Vimentin and keratin intermediate filament systems in cultured PtK2 epithelial cells are interrelated. EMBO J (1982) 1.07

Detection of desmin-containing intermediate filaments in cultured muscle and nonmuscle cells by immunoelectron microscopy. J Cell Biol (1983) 1.05

Distributions of vimentin and desmin in developing chick myotubes in vivo. II. Immunoelectron microscopic study. J Cell Biol (1985) 1.04

Variable expression of desmin in rat glomerular epithelial cells. Am J Pathol (1990) 1.03

Characterization of bovine keratin genes: similarities of exon patterns in genes coding for different keratins. EMBO J (1984) 1.01

Analysis of desmin and vimentin phosphopeptides in cultured avian myogenic cells and their modulation by 8-bromo-adenosine 3',5'-cyclic monophosphate. Proc Natl Acad Sci U S A (1982) 1.01

Human upper epidermal cytoplasmic antibodies are directed against keratin intermediate filament proteins. J Clin Invest (1983) 0.96

Human epithelial cell intermediate filaments: isolation, purification, and characterization. J Cell Biol (1984) 0.90

Canavanine inhibits vimentin assembly but not its synthesis in chicken embryo erythroid cells. J Cell Biol (1983) 0.90

Expression of transfected vimentin genes in differentiating murine erythroleukemia cells reveals divergent cis-acting regulation of avian and mammalian vimentin sequences. Mol Cell Biol (1987) 0.89

Sorting out IF networks: consequences of domain swapping on IF recognition and assembly. J Cell Biol (1991) 0.88

Differential organization of desmin and vimentin in muscle is due to differences in their head domains. J Cell Biol (1994) 0.87

Dynamics of the bacterial intermediate filament crescentin in vitro and in vivo. PLoS One (2010) 0.86

Determination of the critical concentration required for desmin assembly. Biochem J (1990) 0.86

Tissue-specific expression of a vimentin--desmin hybrid gene in transgenic mice. EMBO J (1988) 0.85

Oxidation of thiol in the vimentin cytoskeleton. Biochem J (1991) 0.81

Intermediate Filaments at the Junction of Mechanotransduction, Migration, and Development. Front Cell Dev Biol (2017) 0.75

Articles cited by this

Intermediate filaments as mechanical integrators of cellular space. Nature (1980) 9.98

Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci U S A (1978) 6.28

A simple colorimetric method for determination of protein. Anal Biochem (1969) 4.87

10 nm filaments in normal and transformed cells. Cell (1978) 4.77

Self-assembly of bovine epidermal keratin filaments in vitro. J Mol Biol (1976) 4.43

Studies on the function and composition of the 10-NM(100-A) filaments of vertebrate smooth muscle. J Cell Sci (1977) 4.13

Immunological characterization of the subunit of the 100 A filaments from muscle cells. Proc Natl Acad Sci U S A (1976) 3.99

Keratin cytoskeletons in epithelial cells of internal organs. Proc Natl Acad Sci U S A (1979) 3.45

HeLa cells contain intermediate-sized filaments of the prekeratin type. Exp Cell Res (1979) 2.98

Coexistence of desmin and the fibroblastic intermediate filament subunit in muscle and nonmuscle cells: identification and comparative peptide analysis. Proc Natl Acad Sci U S A (1979) 2.91

The expression of keratin genes in epidermis and cultured epidermal cells. Cell (1978) 2.87

The synthesis and distribution of desmin and vimentin during myogenesis in vitro. Cell (1980) 2.84

Comparison of the genomic organization of Kirsten and Harvey sarcoma viruses. J Virol (1978) 2.66

A micromethod for complete removal of dodecyl sulfate from proteins by ion-pair extraction. Anal Biochem (1979) 2.57

Intermediate filaments of baby hamster kidney (BHK-21) cells and bovine epidermal keratinocytes have similar ultrastructures and subunit domain structures. Proc Natl Acad Sci U S A (1980) 2.45

In vitro assembly of intermediate filaments from baby hamster kidney (BHK-21) cells. Proc Natl Acad Sci U S A (1979) 2.44

Structure of the three-chain unit of the bovine epidermal keratin filament. J Mol Biol (1978) 2.25

X-ray diffraction pattern of axoplasm. Biochim Biophys Acta (1972) 2.24

Neurofilament disguise, destruction and discipline. Nature (1975) 2.19

The polypeptide composition of bovine epidermal alpha-keratin. Biochem J (1975) 2.11

Ten-nanometer filaments of hamster BHK-21 cells and epidermal keratin filaments have similar structures. Proc Natl Acad Sci U S A (1978) 2.01

Analysis of products of mitochondrial protein synthesis in yeast: genetic and biochemical aspects. Methods Enzymol (1979) 1.96

Formation of 100 A filaments from purified glial fibrillary acidic protein in vitro. J Mol Biol (1979) 1.85

Direct demonstration of the presence of two immunologically distinct intermediate-sized filament systems in the same cell by double immunofluorescence microscopy. Vimentin and cytokeratin fibers in cultured epithelial cells. Exp Cell Res (1980) 1.77

Simultaneous expression of two different types of intermediate sized filaments in mouse keratinocytes proliferating in vitro. Differentiation (1979) 1.68

Cytoplasmic fibers in mammalian cells: cytoskeletal and contractile elements. Annu Rev Physiol (1979) 1.63

The extraction and characterization of bovine epidermal alpha-keratin. Biochem J (1975) 1.58

Desmin from avian smooth muscle. Purification and partial characterization. J Biol Chem (1980) 1.49

Phosphorylation of the 10-nm filament protein from Chinese hamster ovary cells. J Biol Chem (1979) 1.46

The detection of smooth muscle desmin-like protein in BHK21/C13 fibroblasts. J Biol Chem (1979) 1.45

A SUGGESTED NOMENCLATURE FOR FINE-STRUCTURAL COMPONENTS OF KERATIN AND KERATIN-LIKE PRODUCTS OF CELLS. Nature (1964) 1.36

Immunological and immuno-fluorescent studies on keratin of the hair follicle. J Cell Sci (1970) 1.25

In vitro reassembly of squid brain intermediate filaments (neurofilaments): purification by assembly-disassembly. Science (1980) 1.21

Characterization of the keratin filament subunits unique to bovine snout epidermis. Biochem J (1980) 1.17

Intermediate filaments from Chinese hamster ovary cells contain a single protein. Comparison with more complex systems from baby hamster kidney and mouse epidermal cells. J Biol Chem (1981) 1.16

Mammalian tyrosinase. Structural and functional interraltionship of isozymes. Biochim Biophys Acta (1978) 1.13

Ultrastructural localization to 10 nm filaments of an insoluble 58K protein in cultured fibroblasts. J Histochem Cytochem (1980) 1.04

Constitutive aggregates of intermediate-sized filaments of the vimentin and cytokeratin type in cultured hepatoma cells and their dispersal by butyrate. Exp Cell Res (1980) 1.00

Articles by these authors

Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell (1986) 8.53

Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues. Proc Natl Acad Sci U S A (1987) 7.38

The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine. J Cell Biol (1971) 6.96

Biochemical, cellular, and pharmacological aspects of the multidrug transporter. Annu Rev Pharmacol Toxicol (1999) 6.62

Expression of a multidrug-resistance gene in human tumors and tissues. Proc Natl Acad Sci U S A (1987) 5.65

Genetics and function of DNA ligase in Escherichia coli. J Mol Biol (1973) 4.52

Self-assembly of bovine epidermal keratin filaments in vitro. J Mol Biol (1976) 4.43

Expression of a full-length cDNA for the human "MDR1" gene confers resistance to colchicine, doxorubicin, and vinblastine. Proc Natl Acad Sci U S A (1987) 4.43

Biochemical and immunological analysis of rapidly purified 10-nm filaments from baby hamster kidney (BHK-21) cells. J Cell Biol (1978) 4.26

The distribution of actin in non-muscle cells. The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells. Exp Cell Res (1975) 3.59

Complete amino acid sequence of a mouse epidermal keratin subunit and implications for the structure of intermediate filaments. Nature (1983) 3.51

Modulation of activity of the promoter of the human MDR1 gene by Ras and p53. Science (1992) 3.41

Dynamic disruptions in nuclear envelope architecture and integrity induced by HIV-1 Vpr. Science (2001) 3.37

The structure of the major cell processes of isolated BHK21 fibroblasts. Exp Cell Res (1969) 3.27

Isolation and genetic characterization of human KB cell lines resistant to multiple drugs. Somat Cell Mol Genet (1985) 3.23

Human multidrug-resistant cell lines: increased mdr1 expression can precede gene amplification. Science (1986) 3.22

Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in vitro. J Cell Biol (1989) 3.21

Isolation of human mdr DNA sequences amplified in multidrug-resistant KB carcinoma cells. Proc Natl Acad Sci U S A (1986) 3.19

The molecular biology of intermediate filaments. Cell (1985) 3.17

Reduced drug accumulation in multiply drug-resistant human KB carcinoma cell lines. Cancer Res (1985) 3.15

The human multidrug resistance (mdr1) gene. cDNA cloning and transcription initiation. J Biol Chem (1987) 3.13

Nuclear lamins A and B1: different pathways of assembly during nuclear envelope formation in living cells. J Cell Biol (2000) 3.06

The occurrence of microvilli during spreading and growth of BHK21-C13 fibroblasts. Exp Cell Res (1970) 2.97

Multiple drug-resistant human KB carcinoma cells independently selected for high-level resistance to colchicine, adriamycin, or vinblastine show changes in expression of specific proteins. J Biol Chem (1986) 2.93

Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution. Proc Natl Acad Sci U S A (1995) 2.89

HIV-1 protease inhibitors are substrates for the MDR1 multidrug transporter. Biochemistry (1998) 2.87

The use of heavy meromyosin binding as an ultrastructural cytochemical method for localizing and determining the possible functions of actin-like microfilaments in nonmuscle cells. J Histochem Cytochem (1975) 2.84

Functions of cytoplasmic fibers in intracellular movements in BHK-21 cells. J Cell Biol (1978) 2.74

Is the multidrug transporter a flippase? Trends Biochem Sci (1992) 2.68

Characterization of bacteriophage lambda reverse as an Escherichia coli phage carrying a unique set of host-derived recombination functions. J Mol Biol (1974) 2.64

Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells. Proc Natl Acad Sci U S A (1977) 2.56

Fluorescent cellular indicators are extruded by the multidrug resistance protein. J Biol Chem (1993) 2.52

The localization of actin-like fibers in cultured neuroblastoma cells as revealed by heavy meromyosin binding. J Cell Biol (1973) 2.50

Intermediate filaments of baby hamster kidney (BHK-21) cells and bovine epidermal keratinocytes have similar ultrastructures and subunit domain structures. Proc Natl Acad Sci U S A (1980) 2.45

Rapid movements of vimentin on microtubule tracks: kinesin-dependent assembly of intermediate filament networks. J Cell Biol (1998) 2.44

In vitro assembly of intermediate filaments from baby hamster kidney (BHK-21) cells. Proc Natl Acad Sci U S A (1979) 2.44

ATP-dependent transport of vinblastine in vesicles from human multidrug-resistant cells. Proc Natl Acad Sci U S A (1988) 2.44

alpha-Galactosidase A deficient mice: a model of Fabry disease. Proc Natl Acad Sci U S A (1997) 2.43

Disruption of nuclear lamin organization alters the distribution of replication factors and inhibits DNA synthesis. J Cell Biol (1997) 2.41

Bricks and mortar of the epidermal barrier. Exp Mol Med (1999) 2.37

Motile properties of vimentin intermediate filament networks in living cells. J Cell Biol (1998) 2.33

A rapid procedure for preparing fluorescein-labeled specific antibodies from whole antiserum: its use in analyzing cytoskeletal architecture. J Cell Biol (1983) 2.32

Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. Proc Natl Acad Sci U S A (1996) 2.31

Localization of multidrug resistance-associated DNA sequences to human chromosome 7. Somat Cell Mol Genet (1986) 2.27

Identification of a major keratinocyte cell envelope protein, loricrin. Cell (1990) 2.25

Structure of the three-chain unit of the bovine epidermal keratin filament. J Mol Biol (1978) 2.25

Dynamic properties of nuclear lamins: lamin B is associated with sites of DNA replication. J Cell Biol (1994) 2.20

Characterization of a class of cationic proteins that specifically interact with intermediate filaments. Proc Natl Acad Sci U S A (1981) 2.15

Structure of the human desmoplakins. Implications for function in the desmosomal plaque. J Biol Chem (1990) 2.13

Single cell analysis of daunomycin uptake and efflux in multidrug-resistant and -sensitive KB cells: effects of verapamil and other drugs. Cancer Res (1986) 2.12

Preservation of normal behavior by enucleated cells in culture. Proc Natl Acad Sci U S A (1973) 2.12

The polypeptide composition of bovine epidermal alpha-keratin. Biochem J (1975) 2.11

Evidence for two nonidentical drug-interaction sites in the human P-glycoprotein. Proc Natl Acad Sci U S A (1997) 2.10

Membrane vesicles from multidrug-resistant human cancer cells contain a specific 150- to 170-kDa protein detected by photoaffinity labeling. Proc Natl Acad Sci U S A (1986) 2.08

Intermediate filament reorganization during mitosis is mediated by p34cdc2 phosphorylation of vimentin. Cell (1990) 2.08

Protein unfolding by peptidylarginine deiminase. Substrate specificity and structural relationships of the natural substrates trichohyalin and filaggrin. J Biol Chem (1996) 2.05

Ten-nanometer filaments of hamster BHK-21 cells and epidermal keratin filaments have similar structures. Proc Natl Acad Sci U S A (1978) 2.01

Certain calcium channel blockers bind specifically to multidrug-resistant human KB carcinoma membrane vesicles and inhibit drug binding to P-glycoprotein. J Biol Chem (1987) 1.99

Mutations in the gene for transglutaminase 1 in autosomal recessive lamellar ichthyosis. Nat Genet (1995) 1.97

Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem (1984) 1.96

Assembly of stratum corneum basic protein and keratin filaments in macrofibrils. Nature (1978) 1.96

Acquisition of a determinant for chloramphenicol resistance by coliphage lambda. Proc Natl Acad Sci U S A (1975) 1.93

Resistance to antimitotic drugs in Chinese hamster ovary cells correlates with changes in the level of polymerized tubulin. J Biol Chem (1991) 1.92

Characterization of human loricrin. Structure and function of a new class of epidermal cell envelope proteins. J Biol Chem (1991) 1.91

Cytoplasmic transport in keratocytes: direct visualization of particle translocation along microtubules. Cell Motil (1983) 1.90

Intermediate filaments: molecular architecture, assembly, dynamics and polymorphism. Q Rev Biophys (1999) 1.89

Immunohistochemical localization in normal tissues of different epitopes in the multidrug transport protein P170: evidence for localization in brain capillaries and crossreactivity of one antibody with a muscle protein. J Histochem Cytochem (1989) 1.89

Organization, structure, and polymorphisms of the human profilaggrin gene. Biochemistry (1990) 1.89

Multidrug resistance of DNA-mediated transformants is linked to transfer of the human mdr1 gene. Mol Cell Biol (1986) 1.89

CHO mutants resistant to colchicine, colcemid or griseofulvin have an altered beta-tubulin. Cell (1980) 1.88

The dynamic properties and possible functions of nuclear lamins. Int Rev Cytol (1995) 1.87

Photosensitized labeling of a functional multidrug transporter in living drug-resistant tumor cells. J Biol Chem (1990) 1.86

Intermediate filaments and the initiation of desmosome assembly. J Cell Biol (1985) 1.84

Dynamic aspects of the supramolecular organization of intermediate filament networks in cultured epidermal cells. Cell Motil (1982) 1.83

The function of intermediate filaments in cell shape and cytoskeletal integrity. J Cell Biol (1996) 1.82

Intermediate filaments: conformity and diversity of expression and structure. Annu Rev Cell Biol (1985) 1.82

Structure of a gene for the human epidermal 67-kDa keratin. Proc Natl Acad Sci U S A (1985) 1.82

Localization and distribution of actin fibers in normal transformed and revertant cells. Cold Spring Harb Symp Quant Biol (1975) 1.82

Birefringent filamentous organelle in BHK-21 cells and its possible role in cell spreading and motility. Science (1970) 1.78

Rapid displacement of vimentin intermediate filaments in living endothelial cells exposed to flow. Circ Res (2000) 1.77

Sjögren-Larsson syndrome is caused by mutations in the fatty aldehyde dehydrogenase gene. Nat Genet (1996) 1.77

Imaging the function of P-glycoprotein with radiotracers: pharmacokinetics and in vivo applications. Clin Pharmacol Ther (2009) 1.77

Human P-glycoprotein exhibits reduced affinity for substrates during a catalytic transition state. Biochemistry (1998) 1.75

Increased vinblastine binding to membrane vesicles from multidrug-resistant KB cells. J Biol Chem (1986) 1.74

The relationship between intermediate filaments and microfilaments before and during the formation of desmosomes and adherens-type junctions in mouse epidermal keratinocytes. J Cell Biol (1987) 1.74

The mdr1 gene, responsible for multidrug-resistance, codes for P-glycoprotein. Biochem Biophys Res Commun (1986) 1.71

Partial purification and reconstitution of the human multidrug-resistance pump: characterization of the drug-stimulatable ATP hydrolysis. Proc Natl Acad Sci U S A (1992) 1.70

Fibrillar systems in cell motility. Ciba Found Symp (1973) 1.70

Microspike-mediated particle transport towards the cell body during early spreading of 3T3 cells. Exp Cell Res (1976) 1.68

Insights into the dynamic properties of keratin intermediate filaments in living epithelial cells. J Cell Biol (2001) 1.66

Pathway of incorporation of microinjected lamin A into the nuclear envelope. J Cell Biol (1992) 1.66