Synthesis of 2'-modified nucleotides and their incorporation into hammerhead ribozymes.

PubWeight™: 0.85‹?›

🔗 View Article (PMC 307401)

Published in Nucleic Acids Res on November 11, 1995

Authors

L Beigelman1, A Karpeisky, J Matulic-Adamic, P Haeberli, D Sweedler, N Usman

Author Affiliations

1: Department of Chemistry and Biochemistry, Ribozyme Pharmaceuticals Inc., Boulder, CO 80301, USA.

Articles cited by this

Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature (1988) 8.74

A small catalytic oligoribonucleotide. Nature (1987) 8.73

Three-dimensional structure of a hammerhead ribozyme. Nature (1994) 7.14

Ribozymes as potential anti-HIV-1 therapeutic agents. Science (1990) 4.49

Synthesis, deprotection, analysis and purification of RNA and ribozymes. Nucleic Acids Res (1995) 3.59

Chemical synthesis of biologically active oligoribonucleotides using beta-cyanoethyl protected ribonucleoside phosphoramidites. Nucleic Acids Res (1990) 3.58

Kinetic characterization of ribonuclease-resistant 2'-modified hammerhead ribozymes. Science (1991) 3.18

Current concepts in antisense drug design. J Med Chem (1993) 2.28

Relationship between 2'-hydroxyls and magnesium binding in the hammerhead RNA domain: a model for ribozyme catalysis. Biochemistry (1991) 1.75

Chemical modification of hammerhead ribozymes. Catalytic activity and nuclease resistance. J Biol Chem (1995) 1.54

Synthesis of some pyrimidine 2'-amino-2'-deoxynucleosides. J Org Chem (1971) 1.52

Minimum ribonucleotide requirement for catalysis by the RNA hammerhead domain. Biochemistry (1992) 1.52

Nuclease-resistant chimeric ribozymes containing deoxyribonucleotides and phosphorothioate linkages. Nucleic Acids Res (1993) 1.44

Exploiting the chemical synthesis of RNA. Trends Biochem Sci (1992) 1.43

Nuclease resistant ribozymes with high catalytic activity. EMBO J (1992) 1.38

Chemically modified RNA: approaches and applications. FASEB J (1993) 1.20

Synthesis and application of derivatizable oligonucleotides. Nucleic Acids Res (1987) 1.14

High activity and stability of hammerhead ribozymes containing 2'-modified pyrimidine nucleosides and phosphorothioates. J Biol Chem (1994) 1.12

Incorporation of 2'-amido-nucleosides in oligodeoxynucleotides and oligoribonucleotides as a model for 2'-linked conjugates. Nucleic Acids Res (1995) 0.99

A conformational basis for the selective action of ara-adenine. J Theor Biol (1977) 0.86

Trichloroacetyl and trifluoroacetyl as N-blocking groups in nucleoside synthesis with 2-amino sugars. J Org Chem (1967) 0.85

Structure and conformation of nucleosides and nucleotides and their analogs as determined by x-ray diffraction. Ann N Y Acad Sci (1975) 0.84

Activity of the hammerhead ribozyme upon inversion of the stereocenters for the guanosine 2'-hydroxyls. Biochemistry (1994) 0.84

Nucleic acids. I. The synthesis of nucleotides and dinucleoside phosphates containing ara-cytidine. J Med Chem (1967) 0.83

NUCLEOSIDES. XXVI. A FACILE SYNTHESIS OF 2,2' -ANHYDRO-ARABINO PYRIMIDINE NUCLEOSIDES. Tetrahedron Lett (1965) 0.78

Articles by these authors

A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res (1984) 181.98

Synthesis, deprotection, analysis and purification of RNA and ribozymes. Nucleic Acids Res (1995) 3.59

Chemical synthesis of biologically active oligoribonucleotides using beta-cyanoethyl protected ribonucleoside phosphoramidites. Nucleic Acids Res (1990) 3.58

Total chemical synthesis of a 77-nucleotide-long RNA sequence having methionine-acceptance activity. Proc Natl Acad Sci U S A (1988) 3.45

Derivatization of controlled pore glass beads for solid phase oligonucleotide synthesis. Biotechniques (1988) 2.93

A ubiquitous family of putative gap junction molecules. Curr Biol (2000) 2.77

Cortical upper layer neurons derive from the subventricular zone as indicated by Svet1 gene expression. Development (2001) 2.37

Labile exocyclic amine protection of nucleosides in DNA, RNA and oligonucleotide analog synthesis facilitating N-deacylation, minimizing depurination and chain degradation. Biochimie (1993) 2.34

The structural basis of hammerhead ribozyme self-cleavage. Cell (1998) 2.20

The molecular structure of the complex of Hoechst 33258 and the DNA dodecamer d(CGCGAATTCGCG). Nucleic Acids Res (1988) 2.07

Characterization of HIV-1 REV protein: binding stoichiometry and minimal RNA substrate. Nucleic Acids Res (1991) 1.96

Specificity for aminoacylation of an RNA helix: an unpaired, exocyclic amino group in the minor groove. Science (1991) 1.96

RNA hydration: a detailed look. Biochemistry (1996) 1.93

Conformational influence of the ribose 2'-hydroxyl group: crystal structures of DNA-RNA chimeric duplexes. Biochemistry (1993) 1.91

Relationship between 2'-hydroxyls and magnesium binding in the hammerhead RNA domain: a model for ribozyme catalysis. Biochemistry (1991) 1.75

Prevention of guanine modification and chain cleavage during the solid phase synthesis of oligonucleotides using phosphoramidite derivatives. Nucleic Acids Res (1986) 1.67

Anticancer effects of gallic acid isolated from Indonesian herbal medicine, Phaleria macrocarpa (Scheff.) Boerl, on human cancer cell lines. Int J Oncol (2007) 1.55

Chemical modification of hammerhead ribozymes. Catalytic activity and nuclease resistance. J Biol Chem (1995) 1.54

Minimum ribonucleotide requirement for catalysis by the RNA hammerhead domain. Biochemistry (1992) 1.52

Enzymatic aminoacylation of single-stranded RNA with an RNA cofactor. Proc Natl Acad Sci U S A (1991) 1.46

Endoscopic ultrasound miniprobe-guided steroid injection for treatment of refractory esophageal strictures. Endoscopy (1997) 1.46

Mixed DNA/RNA polymers are cleaved by the hammerhead ribozyme. Biochemistry (1990) 1.35

The 2,6-diaminopurine riboside.5-methylisocytidine wobble base pair: an isoenergetic substitution for the study of G.U pairs in RNA. Biochemistry (1994) 1.29

Use of binding energy by an RNA enzyme for catalysis by positioning and substrate destabilization. Proc Natl Acad Sci U S A (1995) 1.27

Ribozyme catalysis from the major groove of group II intron domain 5. Mol Cell (1998) 1.24

RhoA and RhoC proteins promote both cell proliferation and cell invasion of human oesophageal squamous cell carcinoma cell lines in vitro and in vivo. Eur J Cancer (2006) 1.20

Rescue of abasic hammerhead ribozymes by exogenous addition of specific bases. Proc Natl Acad Sci U S A (1996) 1.18

Crystal structures of an A-form duplex with single-adenosine bulges and a conformational basis for site-specific RNA self-cleavage. Chem Biol (1996) 1.14

Quantitating tertiary binding energies of 2' OH groups on the P1 duplex of the Tetrahymena ribozyme: intrinsic binding energy in an RNA enzyme. Biochemistry (1997) 1.09

Crystal structure of an Okazaki fragment at 2-A resolution. Proc Natl Acad Sci U S A (1992) 1.09

Functionalized nucleoside 5'-triphosphates for in vitro selection of new catalytic ribonucleic acids. Bioorg Med Chem Lett (2000) 1.08

The crystal structure of r(CCCCGGGG) in two distinct lattices. Biochemistry (1995) 1.04

Template-directed ligation of peptides to oligonucleotides. Chem Biol (1996) 1.03

Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. Biochem Biophys Res Commun (1997) 1.03

Chemically modified hammerhead ribozymes with improved catalytic rates. Biochemistry (1996) 1.03

Base and sugar requirements for RNA cleavage of essential nucleoside residues in internal loop B of the hairpin ribozyme: implications for secondary structure. Nucleic Acids Res (1996) 1.02

Ribozyme-catalyzed primer extension by trinucleotides: a model for the RNA-catalyzed replication of RNA. Biochemistry (1993) 1.00

Branch-site selection in a group II intron mediated by active recognition of the adenine amino group and steric exclusion of non-adenine functionalities. J Mol Biol (1997) 1.00

Chemical synthesis of oligoribonucleotides containing 2-aminopurine: substrates for the investigation of ribozyme function. J Org Chem (1990) 0.97

A core folding model for catalysis by the hammerhead ribozyme accounts for its extraordinary sensitivity to abasic mutations. Biochemistry (1998) 0.97

Template-directed primer extension catalyzed by the Tetrahymena ribozyme. Mol Cell Biol (1991) 0.96

Synthesis of RNA containing inosine: analysis of the sequence requirements for the 5' splice site of the Tetrahymena group I intron. Nucleic Acids Res (1991) 0.93

Effect of structural modifications on the activity of the leadzyme. Biochemistry (1997) 0.93

Optimizing the cell efficacy of synthetic ribozymes. Site selection and chemical modifications of ribozymes targeting the proto-oncogene c-myb. J Biol Chem (1996) 0.92

Ordered differential display: a simple method for systematic comparison of gene expression profiles. Nucleic Acids Res (1997) 0.91

Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface. Nucleic Acids Res (2000) 0.90

Inhibition of vascular smooth muscle cell proliferation by ribozymes that cleave c-myb mRNA. RNA (1996) 0.90

Functional groups on the cleavage site pyrimidine nucleotide are required for stabilization of the hammerhead transition state. RNA (1997) 0.89

X-ray crystallographic observation of "in-line" and "adjacent" conformations in a bulged self-cleaving RNA/DNA hybrid. RNA (2001) 0.88

Base-modified phosphoramidite analogs of pyrimidine ribonucleosides for RNA structure-activity studies. Methods Enzymol (2000) 0.87

1-Deazaadenosine: synthesis and activity of base-modified hammerhead ribozymes. Nucleic Acids Res (1998) 0.87

The synthesis and functional evaluation of RNA and DNA polymers having the sequence of Escherichia coli tRNA(fMet). Eur J Biochem (1989) 0.87

Structural variation induced by different nucleotides at the cleavage site of the hammerhead ribozyme. Biochemistry (1998) 0.86

Cellular uptake properties of a 2'-amino/2'-O-methyl-modified chimeric hammerhead ribozyme targeted to the epidermal growth factor receptor mRNA. Antisense Nucleic Acid Drug Dev (1997) 0.86

Total chemical synthesis of a ribozyme derived from a group I intron. Proc Natl Acad Sci U S A (1995) 0.86

Nucleosides. CXXXV. Synthesis of some 9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purines and their biological activities. Chem Pharm Bull (Tokyo) (1989) 0.84

Role of adenine functional groups in the recognition of the 3'-splice-site AG during the second step of pre-mRNA splicing. Proc Natl Acad Sci U S A (2000) 0.83

An oligodeoxyribonucleotide that supports catalytic activity in the hammerhead ribozyme domain. Nucleic Acids Res (1995) 0.83

Structure-function relationships in the hammerhead ribozyme probed by base rescue. RNA (1998) 0.83

Acute toxicology and pharmacokinetic assessment of a ribozyme (ANGIOZYME) targeting vascular endothelial growth factor receptor mRNA in the cynomolgus monkey. Antisense Nucleic Acid Drug Dev (2000) 0.82

Angiozyme: a novel angiogenesis inhibitor. Curr Oncol Rep (2001) 0.81

Crystal structure of an RNA duplex containing phenyl-ribonucleotides, hydrophobic isosteres of the natural pyrimidines. RNA (2000) 0.81

Specific recognition of an rU2-N15-rU motif by VP55, the vaccinia virus poly(A) polymerase catalytic subunit. J Biol Chem (1997) 0.81

Fluorinated sugar analogues of potential anti-HIV-1 nucleosides. J Med Chem (1991) 0.81

Inductive interactions regulating body patterning in planarian, revealed by analysis of expression of novel gene scarf. Dev Biol (1998) 0.81

Pharmacokinetics and tolerability of an antiangiogenic ribozyme (ANGIOZYME) in healthy volunteers. J Clin Pharmacol (2000) 0.80

Synthesis and biological activities of a phosphorodithioate analog of 2',5'-oligoadenylate. Nucleic Acids Res (1995) 0.79

Synthesis of modified nucleoside 5'-triphosphates for in vitro selection of catalytic nucleic acids. Nucleosides Nucleotides Nucleic Acids (2001) 0.78

A practical method for the production of RNA and ribozymes. Methods Mol Biol (1997) 0.78

Perceptions of influenza and influenza vaccination in patients attending hospital outpatient clinics. Commun Dis Public Health (2002) 0.78

Investigation of the proposed interdomain ribose zipper in hairpin ribozyme cleavage using 2'-modified nucleosides. Biochemistry (2000) 0.77

New neurogenes in mammals. Dokl Biol Sci (2003) 0.75

Site-specific cross-linking of amino acids in the basic region of human immunodeficiency virus type 1 Tat peptide to chemically modified TAR RNA duplexes. Biochemistry (1998) 0.75

Optical spectrum analysis from zero crossings. Opt Lett (1991) 0.75

Nucleosides. CXLIX. Novel and practical synthesis of alpha-monofluoro- and alpha,alpha-difluorothymidine (F-TDR and F2-TDR). Chem Pharm Bull (Tokyo) (1988) 0.75

Crystallization and preliminary X-ray diffraction analysis of double-helical RNA octamers. Acta Crystallogr D Biol Crystallogr (1995) 0.75

Nucleosides. 139. Synthesis and anticytomegalovirus and antiherpes simplex virus activity of 5'-modified analogues of 2'-fluoroarabinosylpyrimidine nucleosides. J Med Chem (1987) 0.75

Synthesis and anti-HIV-1 activity of 2'-"up"-fluoro analogues of active anti-AIDS nucleosides 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (DDC). J Med Chem (1990) 0.75

Two-dimensional image reconstruction from Fourier coefficients computed directly from zero crossings. Appl Opt (1993) 0.75