Methods for separation and deglycosylation of mucin subunits.

PubWeight™: 1.16‹?› | Rank: Top 10%

🔗 View Article (PMID 7668377)

Published in Anal Biochem on May 01, 1995

Authors

D J Thornton1, M Howard, P L Devine, J K Sheehan

Author Affiliations

1: Division of Biochemistry, School of Biological Sciences, University of Manchester, United Kingdom.

Articles citing this

Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense. FASEB J (2009) 2.07

Heterogeneity of airways mucus: variations in the amounts and glycoforms of the major oligomeric mucins MUC5AC and MUC5B. Biochem J (2002) 2.02

Respiratory mucins: identification of core proteins and glycoforms. Biochem J (1996) 1.50

Assay of mucins in human tear fluid. Exp Eye Res (2007) 1.44

Physical characterization of the MUC5AC mucin: a highly oligomeric glycoprotein whether isolated from cell culture or in vivo from respiratory mucous secretions. Biochem J (2000) 1.28

Physical characterization of a low-charge glycoform of the MUC5B mucin comprising the gel-phase of an asthmatic respiratory mucous plug. Biochem J (1999) 1.16

Deglycosylation of glycoproteins with trifluoromethanesulphonic acid: elucidation of molecular structure and function. Biochem J (2003) 1.16

Molecular organization of the mucins and glycocalyx underlying mucus transport over mucosal surfaces of the airways. Mucosal Immunol (2012) 1.10

Mucin degradation mechanisms by distinct Pseudomonas aeruginosa isolates in vitro. Infect Immun (2003) 1.08

Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function. PLoS One (2014) 0.92

Biosynthesis of the MUC2 mucin: evidence for a slow assembly of fully glycosylated units. Biochem J (1996) 0.89

Unpredictable behaviour of mucins in SDS/polyacrylamide-gel electrophoresis. Biochem J (1995) 0.88

A secretory cell type develops alongside multiciliated cells, ionocytes and goblet cells, and provides a protective, anti-infective function in the frog embryonic mucociliary epidermis. Development (2014) 0.86

Mucins secreted by a transformed cell line derived from human tracheal gland cells. Biochem J (1997) 0.86

Sodium dodecyl sulfate agarose gel electropheresis and electroelution of high molecular weight human salivary mucin. Clin Oral Investig (2005) 0.85

Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts. Virol J (2010) 0.85

Salivary mucin 19 glycoproteins: innate immune functions in Streptococcus mutans-induced caries in mice and evidence for expression in human saliva. J Biol Chem (2014) 0.82

Heterogeneity in the protein cores of mucins isolated from human middle ear effusions: evidence for expression of different mucin gene products. Glycoconj J (1998) 0.81

Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Glycoconj J (1998) 0.79

Reorganisation of the salivary mucin network by dietary components: insights from green tea polyphenols. PLoS One (2014) 0.77

Muc5b is the major polymeric mucin in mucus from thoroughbred horses with and without airway mucus accumulation. PLoS One (2011) 0.77

Ligneous conjunctivitis: biochemical evidence for hypofibrinolysis. Inflammation (2000) 0.77

Articles by these authors

IL-10 inhibits cytokine production by activated macrophages. J Immunol (1991) 13.13

IL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells. J Immunol (1991) 11.04

Identification of a T cell-derived b cell growth factor distinct from interleukin 2. J Exp Med (1982) 9.49

Mutation of unique region of Bruton's tyrosine kinase in immunodeficient XID mice. Science (1993) 5.50

Regulation of B-cell growth and differentiation by soluble factors. Annu Rev Immunol (1983) 5.09

School-based programs to reduce sexual risk behaviors: a review of effectiveness. Public Health Rep (1994) 4.50

The biochemistry, biology, and role of interleukin 2 in the induction of cytotoxic T cell and antibody-forming B cell responses. Immunol Rev (1982) 4.43

Interleukin 10, a novel B cell stimulatory factor: unresponsiveness of X chromosome-linked immunodeficiency B cells. J Exp Med (1990) 4.30

Ly-1 B (B-1) cells are the main source of B cell-derived interleukin 10. Eur J Immunol (1992) 3.39

Association of protein kinase A and protein phosphatase 2B with a common anchoring protein. Science (1995) 3.34

Cyclic ADP-ribose production by CD38 regulates intracellular calcium release, extracellular calcium influx and chemotaxis in neutrophils and is required for bacterial clearance in vivo. Nat Med (2001) 3.33

Rapid serologic diagnosis of dengue virus infection using a commercial capture ELISA that distinguishes primary and secondary infections. Am J Trop Med Hyg (1999) 3.11

Isolation and characterization of human cervical-mucus glycoproteins. Biochem J (1983) 3.09

Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli. J Bacteriol (1985) 2.92

Continuous administration of anti-interleukin 10 antibodies delays onset of autoimmunity in NZB/W F1 mice. J Exp Med (1994) 2.71

Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods. Appl Microbiol (1968) 2.58

Evidence for two distinct classes of murine B cell growth factors with activities in different functional assays. J Exp Med (1983) 2.56

Evaluation of a rapid immunochromatographic test for diagnosis of dengue virus infection. J Clin Microbiol (1998) 2.50

The macromolecular structure of human cervical-mucus glycoproteins. Studies on fragments obtained after reduction of disulphide bridges and after subsequent trypsin digestion. Biochem J (1983) 2.49

Evaluation of a commercial enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody in diagnosis of human leptospiral infection. J Clin Microbiol (1997) 2.43

Comparison of self-collected vaginal, vulvar and urine samples with physician-collected cervical samples for human papillomavirus testing to detect high-grade squamous intraepithelial lesions. CMAJ (2000) 2.36

The earliest T lineage-committed cells depend on IL-7 for Bcl-2 expression and normal cell cycle progression. Immunity (1997) 2.33

Immunoglobulin signal transduction guides the specificity of B cell-T cell interactions and is blocked in tolerant self-reactive B cells. J Exp Med (1994) 2.23

Continuous anti-interleukin 10 antibody administration depletes mice of Ly-1 B cells but not conventional B cells. J Exp Med (1992) 2.15

Mucin gene expression during differentiation of human airway epithelia in vitro. Muc4 and muc5b are strongly induced. Am J Respir Cell Mol Biol (1999) 2.10

Use of recombinant envelope proteins for serological diagnosis of Dengue virus infection in an immunochromatographic assay. Clin Diagn Lab Immunol (2001) 2.09

Electron microscopy of cervical, gastric and bronchial mucus glycoproteins. Biochem J (1986) 1.94

Expression of high affinity receptors for murine interleukin 4 (BSF-1) on hemopoietic and nonhemopoietic cells. J Immunol (1988) 1.94

Long-term culture of normal mouse B lymphocytes. Proc Natl Acad Sci U S A (1981) 1.94

Trauma outcome improves following the advanced trauma life support program in a developing country. J Trauma (1993) 1.91

Evaluation of capture ELISA and rapid immunochromatographic test for the determination of IgM and IgG antibodies produced during dengue infection. Clin Diagn Virol (1998) 1.88

Effect of preventive home visits by a nurse on the outcomes of frail elderly people in the community: a randomized controlled trial. CMAJ (2000) 1.86

Prevalence and risk factors for common vision problems in children: data from the ALSPAC study. Br J Ophthalmol (2008) 1.85

Molecular biology of interleukin 4 and interleukin 5 genes and biology of their products that stimulate B cells, T cells and hemopoietic cells. Immunol Rev (1988) 1.84

Mucous glycoproteins: a gel of a problem. Essays Biochem (1985) 1.82

Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3' to 5' exonuclease activity. J Immunol Methods (2000) 1.78

The conformation of the mucopolysaccharides. Hyaluronates. Biochem J (1972) 1.76

Quantitation of mucus glycoproteins blotted onto nitrocellulose membranes. Anal Biochem (1989) 1.75

Biochemical and physicochemical characterization of mouse B cell growth factor: a lymphokine distinct from interleukin 2. J Immunol (1983) 1.73

Interleukin 2 induces antigen-reactive T cell lines to secrete BCGF-I. J Exp Med (1983) 1.71

Role of interleukin 1 in anti-immunoglobulin-induced B cell proliferation. J Exp Med (1983) 1.69

Macromolecular properties and polymeric structure of mucus glycoproteins. Ciba Found Symp (1984) 1.68

Mucus glycoproteins from 'normal' human tracheobronchial secretion. Biochem J (1990) 1.66

Keratinocyte growth factor induces proliferation of hepatocytes and epithelial cells throughout the rat gastrointestinal tract. J Clin Invest (1994) 1.64

B cell growth and differentiation factors. Immunol Rev (1984) 1.61

Postpartum depression in women receiving public assistance: pilot study of an interpersonal-therapy-oriented group intervention. Am J Psychiatry (2001) 1.58

Identification of two glycoforms of the MUC5B mucin in human respiratory mucus. Evidence for a cysteine-rich sequence repeated within the molecule. J Biol Chem (1997) 1.52

Characterization of mucins from cultured normal human tracheobronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol (2000) 1.52

Respiratory mucins: identification of core proteins and glycoforms. Biochem J (1996) 1.50

AKAP79 inhibits calcineurin through a site distinct from the immunophilin-binding region. J Biol Chem (1998) 1.49

Bleeding vascular malformations of the intestine. Surgery (1978) 1.47

Soluble factors involved in B cell differentiation: identification of two distinct T cell-replacing factors (TRF). J Immunol (1983) 1.46

Comprehensive service programs for school-age pregnant girls. Children (1968) 1.44

Hydrodynamic properties of human cervical-mucus glycoproteins in 6M-guanidinium chloride. Biochem J (1984) 1.42

Mucinophilic and chemotactic properties of Pseudomonas aeruginosa in relation to pulmonary colonization in cystic fibrosis. Infect Immun (1990) 1.39

Evaluation of a commercial capture enzyme-linked immunosorbent assay for detection of immunoglobulin M and G antibodies produced during dengue infection. Clin Diagn Lab Immunol (1998) 1.39

Plasma concentrations of digoxin after oral administration in the fasting and postprandial ste. Br Med J (1971) 1.36

The Eczema Priority Setting Partnership: a collaboration between patients, carers, clinicians and researchers to identify and prioritize important research questions for the treatment of eczema. Br J Dermatol (2013) 1.34

Cyclin D2 is essential for BCR-mediated proliferation and CD5 B cell development. Int Immunol (2000) 1.33

Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Glycobiology (1999) 1.32

Is the macromolecular architecture of cervical, respiratory and gastric mucins the same? Biochem Soc Trans (1984) 1.29

Safe at home? Domestic violence and other homicides among women in New Mexico. Ann Emerg Med (1996) 1.28

Physical characterization of the MUC5AC mucin: a highly oligomeric glycoprotein whether isolated from cell culture or in vivo from respiratory mucous secretions. Biochem J (2000) 1.28

Mucins in airway secretions from healthy and chronic bronchitic subjects. Biochem J (1996) 1.25

Interaction between heparan sulphate chains. I. A gel chromatographic, light-scattering and structural study of aggregating and non-aggregating chains. Biochim Biophys Acta (1980) 1.23

Interleukin 4 is a growth factor for activated thymocytes: possible role in T-cell ontogeny. Proc Natl Acad Sci U S A (1987) 1.22

Comparison of PanBio dengue duo enzyme-linked immunosorbent assay (ELISA) and MRL dengue fever virus immunoglobulin M capture ELISA for diagnosis of dengue virus infections in Southeast Asia. Clin Diagn Lab Immunol (1999) 1.21

The left atrial appendage: our most lethal human attachment! Surgical implications. Eur J Cardiothorac Surg (2000) 1.21

Structure and macromolecular properties of cervical mucus glycoproteins. Symp Soc Exp Biol (1989) 1.20

Analysis of respiratory mucus glycoproteins in asthma: a detailed study from a patient who died in status asthmaticus. Am J Respir Cell Mol Biol (1995) 1.17

Cloning and expression of a cDNA coding for a human monocyte-derived plasminogen activator inhibitor. Proc Natl Acad Sci U S A (1988) 1.17

Structure for hyaluronic acid. Nat New Biol (1972) 1.17

Chlamydial cervicitis: testing the practice guidelines for presumptive diagnosis. CMAJ (1998) 1.17

Expression cloning of a cDNA encoding the murine interleukin 4 receptor based on ligand binding. Proc Natl Acad Sci U S A (1990) 1.17

Modified immunological status of anti-IL-10 treated mice. Cell Immunol (1993) 1.17

Assessment of the cervix after acetic acid wash: inter-rater agreement using photographs. Obstet Gynecol (2002) 1.16

Mucus glycoproteins from cystic fibrotic sputum. Macromolecular properties and structural 'architecture'. Biochem J (1991) 1.16

Physical characterization of a low-charge glycoform of the MUC5B mucin comprising the gel-phase of an asthmatic respiratory mucous plug. Biochem J (1999) 1.16

Evaluation of a capture screening enzyme-linked immunosorbent assay for combined determination of immunoglobulin M and G antibodies produced during Dengue infection. Clin Diagn Lab Immunol (2000) 1.15

Myocardial and skeletal muscle concentrations of digoxin in patients on long-term therapy. Br Med J (1972) 1.14

Identification of two major populations of mucins in respiratory secretions. Am J Respir Crit Care Med (1994) 1.13

Patient choice of treatment for postpartum depression: a pilot study. Arch Womens Ment Health (2006) 1.12

Equilibrium-binding studies of pig laryngeal cartilage proteoglycans with hyaluronate oligosaccharide fractions. Biochem J (1980) 1.12

T-cell hybridoma secreting hemopoietic regulatory molecules: granulocyte-macrophage and eosinophil colony-stimulating factors. Cell (1979) 1.11

Molecular organization of the mucins and glycocalyx underlying mucus transport over mucosal surfaces of the airways. Mucosal Immunol (2012) 1.10

Identification in vitreous and molecular cloning of opticin, a novel member of the family of leucine-rich repeat proteins of the extracellular matrix. J Biol Chem (2000) 1.10

Hyaluronan: polysaccharide chaos to protein organisation. Curr Opin Struct Biol (2001) 1.09

Early diagnosis of scrub typhus with a rapid flow assay using recombinant major outer membrane protein antigen (r56) of Orientia tsutsugamushi. Clin Diagn Lab Immunol (2001) 1.09

Management of Acanthamoeba keratitis. A case report and review of the literature. Ophthalmology (1984) 1.09

Trauma mannequin assessment of management skills of surgical residents after advanced trauma life support training. J Surg Res (2000) 1.09

Mucin expression by transitional cell carcinomas of the bladder. Br J Urol (1994) 1.08

The work-related fatal injury study: numbers, rates and trends of work-related fatal injuries in New Zealand 1985-1994. N Z Med J (2001) 1.08

Activated B cells from patients with common variable immunodeficiency proliferate and synthesize immunoglobulin. J Clin Invest (1993) 1.07