Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains.

PubWeight™: 3.01‹?› | Rank: Top 1%

🔗 View Article (PMC 262614)

Published in J Clin Microbiol on January 01, 1993

Authors

O Olsvik1, J Wahlberg, B Petterson, M Uhlén, T Popovic, I K Wachsmuth, P I Fields

Author Affiliations

1: Division of Bacterial and Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.

Articles citing this

Cholera. Clin Microbiol Rev (1995) 12.33

A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J Clin Microbiol (1998) 4.79

Cholera due to altered El Tor strains of Vibrio cholerae O1 in Bangladesh. J Clin Microbiol (2006) 3.60

Epidemiologic application of a standardized ribotype scheme for Vibrio cholerae O1. J Clin Microbiol (1993) 3.59

Vibrio cholerae O1 hybrid El Tor strains, Asia and Africa. Emerg Infect Dis (2008) 2.53

Comparative genomics of Vibrio cholerae from Haiti, Asia, and Africa. Emerg Infect Dis (2011) 2.50

Magnetic separation techniques in diagnostic microbiology. Clin Microbiol Rev (1994) 2.41

Virulence genes in environmental strains of Vibrio cholerae. Appl Environ Microbiol (2000) 2.21

Characterization of toxigenic Vibrio cholerae from Haiti, 2010-2011. Emerg Infect Dis (2011) 2.08

Vibrio cholerae O139 Bengal. J Clin Microbiol (1994) 2.07

Genetic diversity and population structure of Vibrio cholerae. J Clin Microbiol (1999) 2.00

Detection of RTX toxin gene in Vibrio cholerae by PCR. J Clin Microbiol (2001) 1.84

Incidence, virulence factors, and clonality among clinical strains of non-O1, non-O139 Vibrio cholerae isolates from hospitalized diarrheal patients in Kolkata, India. J Clin Microbiol (2009) 1.82

Evolutionary relationships of pathogenic clones of Vibrio cholerae by sequence analysis of four housekeeping genes. Infect Immun (1999) 1.73

Classical ctxB in Vibrio cholerae O1, Kolkata, India. Emerg Infect Dis (2009) 1.53

Heterogeneity of diphtheria toxin gene, tox, and its regulatory element, dtxR, in Corynebacterium diphtheriae strains causing epidemic diphtheria in Russia and Ukraine. J Clin Microbiol (1996) 1.48

Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence. Proc Natl Acad Sci U S A (2008) 1.31

Cholera toxin - a foe & a friend. Indian J Med Res (2011) 1.31

Etiology of children's diarrhea in Montevideo, Uruguay: associated pathogens and unusual isolates. J Clin Microbiol (2001) 1.25

Development and evaluation of a PCR assay for tracking the emergence and dissemination of Haitian variant ctxB in Vibrio cholerae O1 strains isolated from Kolkata, India. J Clin Microbiol (2012) 1.13

Molecular characterization of high-level-cholera-toxin-producing El Tor variant Vibrio cholerae strains in the Zanzibar Archipelago of Tanzania. J Clin Microbiol (2013) 1.08

Molecular evidence of cholera outbreak caused by a toxigenic Vibrio cholerae O1 El tor variant strain in Kelantan, Malaysia. J Clin Microbiol (2010) 1.06

Cholera epidemiology in Nigeria: an overview. Pan Afr Med J (2012) 1.03

Genetic characteristics of drug-resistant Vibrio cholerae O1 causing endemic cholera in Dhaka, 2006-2011. J Med Microbiol (2012) 1.02

New V. cholerae atypical El Tor variant emerged during the 2006 epidemic outbreak in Angola. BMC Microbiol (2011) 0.99

Genetic traits of Vibrio cholerae O1 Haitian isolates that are absent in contemporary strains from Kolkata, India. PLoS One (2014) 0.98

A reference pan-genome approach to comparative bacterial genomics: identification of novel epidemiological markers in pathogenic Campylobacter. PLoS One (2014) 0.97

Molecular characterization of Vibrio cholerae outbreak strains with altered El Tor biotype from southern India. World J Microbiol Biotechnol (2009) 0.97

Molecular analyses of Vibrio cholerae O1 clinical strains, including new nontoxigenic variants isolated in Mexico during the Cholera epidemic years between 1991 and 2000. J Clin Microbiol (2009) 0.95

A brief review of molecular techniques to assess plant diversity. Int J Mol Sci (2010) 0.94

Vibrio cholerae classical biotype strains reveal distinct signatures in Mexico. J Clin Microbiol (2012) 0.94

Cholera outbreaks (2012) in three districts of Nepal reveal clonal transmission of multi-drug resistant Vibrio cholerae O1. BMC Infect Dis (2014) 0.90

Distribution of virulence-associated genes and genetic relationships in non-O1/O139 Vibrio cholerae aquatic isolates from China. Appl Environ Microbiol (2014) 0.88

Sequence polymorphisms of rfbT among the Vibrio cholerae O1 strains in the Ogawa and Inaba serotype shifts. BMC Microbiol (2013) 0.87

Hybrid El Tor Vibrio cholerae O1, Kuwait. Emerg Infect Dis (2009) 0.86

Synergistic effect of various virulence factors leading to high toxicity of environmental V. cholerae non-O1/ non-O139 isolates lacking ctx gene : comparative study with clinical strains. PLoS One (2013) 0.85

A cholera outbreak of the Vibrio cholerae O1 El Tor variant carrying classical CtxB in northeastern Thailand in 2007. Am J Trop Med Hyg (2010) 0.84

Outbreak-associated Vibrio cholerae genotypes with identical pulsotypes, Malaysia, 2009. Emerg Infect Dis (2012) 0.81

Clonal Dissemination of a Single Vibrio cholerae O1 Biotype El Tor Strain in Sistan-Baluchestan Province of Iran During 2013. Curr Microbiol (2015) 0.80

Towards a structural basis for the relationship between blood group and the severity of El Tor cholera. Angew Chem Int Ed Engl (2012) 0.80

Binding efficiencies of carbohydrate ligands with different genotypes of cholera toxin B: molecular modeling, dynamics and docking simulation studies. J Mol Model (2011) 0.78

Immunization with cholera toxin B subunit induces high-level protection in the suckling mouse model of cholera. PLoS One (2013) 0.78

The seventh pandemic Vibrio cholerae O1 El Tor isolate in China has undergone genetic shifts. J Clin Microbiol (2013) 0.77

Structural inferences for Cholera toxin mutations in Vibrio cholerae. Bioinformation (2011) 0.77

Risk of Vibrio transmission linked to the consumption of crustaceans in coastal towns of Côte d'Ivoire. J Food Prot (2012) 0.77

Vibrio cholerae O1 El Tor variant and emergence of Haitian ctxB variant in the strains isolated from South India. Med Microbiol Immunol (2015) 0.76

Drug response and genetic properties of Vibrio cholerae associated with endemic cholera in north-eastern Thailand, 2003-2011. J Med Microbiol (2013) 0.76

Association of Heavy Rainfall on Genotypic Diversity in V. cholerae Isolates from an Outbreak in India. Int J Microbiol (2011) 0.76

Phenotypic and Genetic Heterogeneity in Vibrio cholerae O139 Isolated from Cholera Cases in Delhi, India during 2001-2006. Front Microbiol (2016) 0.75

Electrophoresis-enhanced detection of deoxyribonucleic acids on a membrane-based lateral flow strip using avian influenza H5 genetic sequence as the model. Sensors (Basel) (2014) 0.75

Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India. PLoS Negl Trop Dis (2017) 0.75

Novel multiple mutations in the topoisomerase gene of Haitian variant Vibrio cholerae O1. Antimicrob Agents Chemother (2014) 0.75

Articles cited by this

Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature (1984) 12.10

Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res (1989) 9.04

Duplication and amplification of toxin genes in Vibrio cholerae. Cell (1983) 8.53

Field trial of oral cholera vaccines in Bangladesh: results from three-year follow-up. Lancet (1990) 4.62

Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J Clin Microbiol (1992) 3.10

Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin. J Biol Chem (1983) 2.20

Cholera toxin: genetic analysis, regulation, and role in pathogenesis. Curr Top Microbiol Immunol (1985) 1.89

Difference between toxigenic Vibrio cholerae O1 from South America and US gulf coast. Lancet (1991) 1.87

Isolation of Latin American epidemic strain of Vibrio cholerae O1 from US Gulf Coast. Lancet (1992) 1.80

Genetic diversity among toxigenic and nontoxigenic Vibrio cholerae O1 isolated from the Western Hemisphere. Epidemiol Infect (1991) 1.61

Toxigenic Vibrio cholerae O1 and cargo ships entering Gulf of Mexico. Lancet (1992) 1.51

Analysis of structure and function of the B subunit of cholera toxin by the use of site-directed mutagenesis. Mol Microbiol (1991) 1.50

Dynamic changes in HIV-1 quasispecies from azidothymidine (AZT)-treated patients. FASEB J (1992) 1.41

Variations in the cytomegalovirus major immediate-early gene found by direct genomic sequencing. J Clin Microbiol (1992) 1.22

Purification of El Tor cholera enterotoxins and comparisons with classical toxin. J Gen Microbiol (1990) 1.16

Study of epitopes of cholera enterotoxin-related enterotoxins by checkerboard immunoblotting. Infect Immun (1990) 1.06

Mapping epitopic regions of cholera toxin B-subunit protein. Mol Immunol (1991) 1.04

Variation in epitopes of the B subunit of El Tor and classical biotype Vibrio cholerae O1 cholera toxin. J Gen Microbiol (1989) 0.96

Articles by these authors

A sequencing method based on real-time pyrophosphate. Science (1998) 21.21

Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent. Proc Natl Acad Sci U S A (1986) 14.31

Real-time DNA sequencing using detection of pyrophosphate release. Anal Biochem (1996) 12.94

Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States. Emerg Infect Dis (2001) 11.73

Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J Clin Microbiol (1983) 10.23

Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res (1989) 9.04

Meningococcal disease. N Engl J Med (2001) 8.50

Solid phase DNA minisequencing by an enzymatic luminometric inorganic pyrophosphate detection assay. Anal Biochem (1993) 7.71

Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea. Infect Immun (1993) 6.72

Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications. J Biol Chem (1984) 5.94

Campylobacter jejuni--an emerging foodborne pathogen. Emerg Infect Dis (1999) 5.83

Isolation of Escherichia coli O157:H7 from dairy cattle associated with two cases of haemolytic uraemic syndrome. Lancet (1986) 4.44

Toward a population genetic analysis of Salmonella: genetic diversity and relationships among strains of serotypes S. choleraesuis, S. derby, S. dublin, S. enteritidis, S. heidelberg, S. infantis, S. newport, and S. typhimurium. Proc Natl Acad Sci U S A (1988) 4.43

A high frequency of sequence alterations is due to formalin fixation of archival specimens. Am J Pathol (1999) 4.43

The changing epidemiology of meningococcal disease in the United States, 1992-1996. J Infect Dis (1999) 4.37

Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis (1988) 4.12

Salmonellosis associated with marijuana: a multistate outbreak traced by plasmid fingerprinting. N Engl J Med (1982) 4.03

The pathogenesis of hemorrhagic colitis caused by Escherichia coli O157:H7 in gnotobiotic piglets. J Infect Dis (1986) 3.96

Infantile diarrhea produced by heat-stable enterotoxigenic Escherichia coli. N Engl J Med (1976) 3.82

Production of Shiga-like toxin by Escherichia coli. J Infect Dis (1986) 3.53

Comparison of plasmid profile analysis, phage typing, and antimicrobial susceptibility testing in characterizing Salmonella typhimurium isolates from outbreaks. J Clin Microbiol (1984) 3.33

Gene discovery in the wood-forming tissues of poplar: analysis of 5, 692 expressed sequence tags. Proc Natl Acad Sci U S A (1998) 3.31

A synthetic IgG-binding domain based on staphylococcal protein A. Protein Eng (1989) 3.31

Molecular characterization of Vibrio cholerae O1 strains by pulsed-field gel electrophoresis. J Clin Microbiol (1994) 3.27

The molecular epidemiology of cholera in Latin America. J Infect Dis (1993) 3.26

Non-O group 1 Vibrio cholerae gastroenteritis in the United States: clinical, epidemiologic, and laboratory characteristics of sporadic cases. Ann Intern Med (1981) 3.25

Single-nucleotide polymorphism analysis by pyrosequencing. Anal Biochem (2000) 3.21

Rapid development of ciprofloxacin resistance in methicillin-susceptible and -resistant Staphylococcus aureus. J Infect Dis (1991) 3.12

Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J Clin Microbiol (1992) 3.10

Discrimination of Campylobacter jejuni isolates by fla gene sequencing. J Clin Microbiol (1997) 3.08

Region X, the cell-wall-attachment part of staphylococcal protein A. Eur J Biochem (1984) 3.00

Affinity purification and characterization of Shiga-like toxin II and production of toxin-specific monoclonal antibodies. Infect Immun (1988) 2.99

Evaluation of 10 methods to distinguish epidemic-associated Campylobacter strains. J Clin Microbiol (1991) 2.97

Gene for staphylococcal protein A. Proc Natl Acad Sci U S A (1983) 2.97

Analysis of eight cases of neonatal meningitis and sepsis due to Enterobacter sakazakii. J Clin Microbiol (1983) 2.96

High-molecular-weight plasmid correlates with Escherichia coli enteroinvasiveness. Infect Immun (1982) 2.95

Properties of Bacillus subtilis 168 derivatives freed of their natural prophages. Gene (1980) 2.91

Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors. EMBO J (1985) 2.91

A transcriptional roadmap to wood formation. Proc Natl Acad Sci U S A (2001) 2.85

Epidemic diarrhea at Crater Lake from enterotoxigenic Escherichia coli. A large waterborne outbreak. Ann Intern Med (1977) 2.80

Identification of the causative agent of granulocytic ehrlichiosis in Swedish dogs and horses by direct solid phase sequencing of PCR products from the 16S rRNA gene. Res Vet Sci (1995) 2.77

Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA. Biotechniques (1991) 2.69

An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res (1983) 2.69

Fit genotypes and escape variants of subgroup III Neisseria meningitidis during three pandemics of epidemic meningitis. Proc Natl Acad Sci U S A (2001) 2.66

Analysis of heterogeneous viral populations by direct DNA sequencing. Biotechniques (1993) 2.65

Virulence factors in Shiga-like toxin-producing Escherichia coli isolated from humans and cattle. J Infect Dis (1992) 2.62

Accurate reconstruction of a known HIV-1 transmission history by phylogenetic tree analysis. Proc Natl Acad Sci U S A (1996) 2.57

Comparison of variable region 3 sequences of human immunodeficiency virus type 1 from infected children with the RNA and DNA sequences of the virus populations of their mothers. Proc Natl Acad Sci U S A (1993) 2.49

Parasitic, bacterial, and viral enteric pathogens associated with diarrhea in the Central African Republic. J Clin Microbiol (1984) 2.46

Unusual verotoxin-producing Escherichia coli associated with hemorrhagic colitis. J Clin Microbiol (1987) 2.44

Magnetic separation techniques in diagnostic microbiology. Clin Microbiol Rev (1994) 2.41

A novel fusion gene, SYT-SSX4, in synovial sarcoma. J Natl Cancer Inst (1999) 2.36

Serotype-specific prevalence of Escherichia coli strains with EPEC adherence factor genes in infants with and without diarrhea in São Paulo, Brazil. J Infect Dis (1989) 2.35

Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain. Nat Biotechnol (1997) 2.21

Structure of the IgG-binding regions of streptococcal protein G. EMBO J (1986) 2.20

Resistance mechanisms of multiply resistant pneumococci: antibiotic degradation studies. Antimicrob Agents Chemother (1979) 2.17

Improved cortisol exposure-time profile and outcome in patients with adrenal insufficiency: a prospective randomized trial of a novel hydrocortisone dual-release formulation. J Clin Endocrinol Metab (2011) 2.16

Campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness. J Clin Microbiol (1991) 2.15

Molecular epidemiology of diphtheria in Russia, 1985-1994. J Infect Dis (1996) 2.12

Biological and molecular characterization of subtype D, G, and A/D recombinant HIV-1 transmissions in Sweden. Virology (1995) 2.07

Polymorphism in Bordetella pertussis pertactin and pertussis toxin virulence factors in the United States, 1935-1999. J Infect Dis (2000) 2.05

Aeromonas intestinal infections in the United States. Ann Intern Med (1986) 2.03

Helicobacter cinaedi-associated bacteremia and cellulitis in immunocompromised patients. Ann Intern Med (1994) 2.01

The refined 2.15 A X-ray crystal structure of human liver cathepsin B: the structural basis for its specificity. EMBO J (1991) 1.99

Neisseria meningitidis serogroup W135 isolates associated with the ET-37 complex. Emerg Infect Dis (2000) 1.97

Current approaches to the laboratory diagnosis of diphtheria. J Infect Dis (2000) 1.95

Specific binding of proinsulin C-peptide to human cell membranes. Proc Natl Acad Sci U S A (1999) 1.91

Analysis of a rape case by direct sequencing of the human immunodeficiency virus type 1 pol and gag genes. J Virol (1994) 1.88

Difference between toxigenic Vibrio cholerae O1 from South America and US gulf coast. Lancet (1991) 1.87

Epidemiologic typing of Staphylococcus aureus by DNA restriction fragment length polymorphisms of rRNA genes: elucidation of the clonal nature of a group of bacteriophage-nontypeable, ciprofloxacin-resistant, methicillin-susceptible S. aureus isolates. J Clin Microbiol (1992) 1.86

Crystal structure of the C2 fragment of streptococcal protein G in complex with the Fc domain of human IgG. Structure (1995) 1.86

Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr Purif (1997) 1.84

Sequence analysis of 16S rRNA from mycoplasmas by direct solid-phase DNA sequencing. Appl Environ Microbiol (1994) 1.81

Molecular epidemiology of Yersinia enterocolitica O:3 infections: use of chromosomal DNA restriction fragment length polymorphisms of rRNA genes. J Clin Microbiol (1991) 1.80

Isolation of Latin American epidemic strain of Vibrio cholerae O1 from US Gulf Coast. Lancet (1992) 1.80

Influence of the 60-megadalton plasmid on adherence of Escherichia coli O157:H7 and genetic derivatives. Infect Immun (1990) 1.80

Restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant Campylobacter species. J Clin Microbiol (1991) 1.77

Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian diphtheria epidemic, 1990 through 1994. J Clin Microbiol (1995) 1.76

Analysis of signals for secretion in the staphylococcal protein A gene. EMBO J (1985) 1.72

Uniformly spaced banding pattern in DNA sequencing gels by use of field-strength gradient. J Biochem Biophys Methods (1984) 1.71

Sotrastaurin, a novel small molecule inhibiting protein-kinase C: randomized phase II study in renal transplant recipients. Am J Transplant (2011) 1.69

Vibriophage VcA-3 as an epidemic strain marker for the U.S. Gulf Coast Vibrio cholerae O1 clone. J Clin Microbiol (1992) 1.68

Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides. Clin Chem (1995) 1.67

Plasmid-mediated properties of a heat-stable enterotoxin-producing Escherichia coli associated with infantile diarrhea. Infect Immun (1976) 1.67

What constitutes cerebral palsy? Dev Med Child Neurol (1998) 1.66

Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group. Int J Syst Bacteriol (1996) 1.64

Overexpression of podocalyxin-like protein is an independent factor of poor prognosis in colorectal cancer. Br J Cancer (2011) 1.64

Restriction fragment length polymorphisms in rRNA operons for subtyping Shigella sonnei. J Clin Microbiol (1991) 1.63

Genotypic and phenotypic characterization of Helicobacter cinaedi and Helicobacter fennelliae strains isolated from humans and animals. J Clin Microbiol (1995) 1.62

Gene fusion vectors based on the gene for staphylococcal protein A. Gene (1983) 1.61

Expression of the gene encoding protein A in Staphylococcus aureus and coagulase-negative staphylococci. J Bacteriol (1984) 1.61

Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. Nucleic Acids Res (1985) 1.61