Published in Protein Eng on September 01, 1994
Image-guided cancer surgery using near-infrared fluorescence. Nat Rev Clin Oncol (2013) 3.01
Properties, production, and applications of camelid single-domain antibody fragments. Appl Microbiol Biotechnol (2007) 2.59
Single-domain antibody fragments with high conformational stability. Protein Sci (2002) 2.31
Potent enzyme inhibitors derived from dromedary heavy-chain antibodies. EMBO J (1998) 2.30
Beta-lactamase inhibitors derived from single-domain antibody fragments elicited in the camelidae. Antimicrob Agents Chemother (2001) 2.24
Structural analysis of the nurse shark (new) antigen receptor (NAR): molecular convergence of NAR and unusual mammalian immunoglobulins. Proc Natl Acad Sci U S A (1998) 1.42
Alpaca (Lama pacos) as a convenient source of recombinant camelid heavy chain antibodies (VHHs). J Immunol Methods (2007) 1.36
Camel heavy-chain antibodies: diverse germline V(H)H and specific mechanisms enlarge the antigen-binding repertoire. EMBO J (2000) 1.28
General strategy for the generation of human antibody variable domains with increased aggregation resistance. Proc Natl Acad Sci U S A (2012) 1.17
A case of convergence: why did a simple alternative to canonical antibodies arise in sharks and camels? PLoS Biol (2011) 1.07
Structural evidence for evolution of shark Ig new antigen receptor variable domain antibodies from a cell-surface receptor. Proc Natl Acad Sci U S A (2004) 1.06
Structural evaluation of EGFR inhibition mechanisms for nanobodies/VHH domains. Structure (2013) 1.01
Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents. PLoS One (2010) 1.01
Affinity transfer by CDR grafting on a nonimmunoglobulin scaffold. Protein Sci (2004) 0.97
Nanobodies and recombinant binders in cell biology. J Cell Biol (2015) 0.96
On the genomics of immunoglobulins in the gray, short-tailed opossum Monodelphis domestica. Immunogenetics (2009) 0.95
Thermostable llama single domain antibodies for detection of botulinum A neurotoxin complex. Anal Chem (2008) 0.95
Nanobodies as novel agents for disease diagnosis and therapy. Int J Nanomedicine (2013) 0.93
Dual beneficial effect of interloop disulfide bond for single domain antibody fragments. J Biol Chem (2011) 0.93
Intraoperative fluorescence delineation of head and neck cancer with a fluorescent anti-epidermal growth factor receptor nanobody. Int J Cancer (2013) 0.92
Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications. Int J Nanomedicine (2016) 0.91
Tumor-penetrating peptide fused EGFR single-domain antibody enhances cancer drug penetration into 3D multicellular spheroids and facilitates effective gastric cancer therapy. J Control Release (2014) 0.91
Flexibility and small pockets at protein-protein interfaces: New insights into druggability. Prog Biophys Mol Biol (2015) 0.90
Cell penetrable humanized-VH/V(H)H that inhibit RNA dependent RNA polymerase (NS5B) of HCV. PLoS One (2012) 0.90
The smart targeting of nanoparticles. Curr Pharm Des (2013) 0.89
Novel antibody derivatives for proteome and high-content analysis. Anal Bioanal Chem (2010) 0.88
Botulinum neurotoxins and botulism: a novel therapeutic approach. Toxins (Basel) (2011) 0.86
Utilizing nanobody technology to target non-immunodominant domains of VAR2CSA. PLoS One (2014) 0.86
Relevance of the diversity among members of the Trypanosoma cruzi trans-sialidase family analyzed with camelids single-domain antibodies. PLoS One (2008) 0.86
Radiolabeled nanobodies as theranostic tools in targeted radionuclide therapy of cancer. Expert Opin Drug Deliv (2014) 0.85
Fluorescent labelling of the actin cytoskeleton in plants using a cameloid antibody. Plant Methods (2014) 0.85
Isolation and epitope mapping of staphylococcal enterotoxin B single-domain antibodies. Sensors (Basel) (2014) 0.84
High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli. Microb Cell Fact (2013) 0.84
Isolation of alpaca anti-idiotypic heavy-chain single-domain antibody for the aflatoxin immunoassay. Anal Chem (2013) 0.84
Heavy-chain only antibodies derived from dromedary are secreted and displayed by mouse B cells. Immunology (2003) 0.83
Current progress and challenges in HIV gene therapy. Future Virol (2011) 0.82
Rugged single domain antibody detection elements for Bacillus anthracis spores and vegetative cells. PLoS One (2012) 0.82
Characterization of Asia 1 sdAb from camels bactrianus (C. bactrianus) and conjugation with quantum dots for imaging FMDV in BHK-21 cells. PLoS One (2013) 0.82
Camelid Ig V genes reveal significant human homology not seen in therapeutic target genes, providing for a powerful therapeutic antibody platform. MAbs (2015) 0.81
Functional inhibition of β-catenin-mediated Wnt signaling by intracellular VHH antibodies. MAbs (2015) 0.80
Evaluation of antidiphtheria toxin nanobodies. Nanotechnol Sci Appl (2010) 0.79
Design and selection of a camelid single-chain antibody yeast two-hybrid library produced de novo for the cap protein of porcine circovirus type 2 (PCV2). PLoS One (2013) 0.79
Affinity transfer to a human protein by CDR3 grafting of camelid VHH. Protein Sci (2011) 0.79
Strategies to stabilize compact folding and minimize aggregation of antibody-based fragments. Adv Biosci Biotechnol (2013) 0.79
Fully Human VH Single Domains That Rival the Stability and Cleft Recognition of Camelid Antibodies. J Biol Chem (2015) 0.78
A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma. Int J Mol Sci (2014) 0.78
Construction of a camelid VHH yeast two-hybrid library and the selection of VHH against haemagglutinin-neuraminidase protein of the Newcastle disease virus. BMC Vet Res (2016) 0.78
Analysis and modeling of the variable region of camelid single-domain antibodies. J Immunol (2011) 0.78
Novel camelid antibody fragments targeting recombinant nucleoprotein of Araucaria hantavirus: a prototype for an early diagnosis of Hantavirus Pulmonary Syndrome. PLoS One (2014) 0.77
Immuno-targeting the multifunctional CD38 using nanobody. Sci Rep (2016) 0.77
Beyond peptides and mAbs--current status and future perspectives for biotherapeutics with novel constructs. J Clin Pharmacol (2015) 0.76
Contributions of conventional and heavy-chain IgG to immunity in fetal, neonatal, and adult alpacas. Clin Vaccine Immunol (2010) 0.76
The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins. Proc Natl Acad Sci U S A (2014) 0.76
Distinct antibody species: structural differences creating therapeutic opportunities. Curr Opin Immunol (2016) 0.76
Antibody Fragments as Probe in Biosensor Development. Sensors (Basel) (2008) 0.75
High-Throughput Ligand Discovery Reveals a Sitewise Gradient of Diversity in Broadly Evolved Hydrophilic Fibronectin Domains. PLoS One (2015) 0.75
Selective function-blocking monoclonal human antibody highlights the important role of membrane type-1 matrix metalloproteinase (MT1-MMP) in metastasis. Oncotarget (2016) 0.75
Comparative Analysis of Immune Repertoires between Bactrian Camel's Conventional and Heavy-Chain Antibodies. PLoS One (2016) 0.75
Blocking of Histamine Release and IgE Binding to FcεRI on Human Basophils by Antibodies Produced in Camels. Allergy Asthma Immunol Res (2015) 0.75
Naturally occurring antibodies devoid of light chains. Nature (1993) 7.35
Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci U S A (1980) 5.47
Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present. Ann Trop Med Parasitol (2003) 3.48
Selection and identification of single domain antibody fragments from camel heavy-chain antibodies. FEBS Lett (1997) 3.18
A VSG expression site-associated gene confers resistance to human serum in Trypanosoma rhodesiense. Cell (1998) 3.08
Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme. Nat Struct Biol (1996) 2.54
HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides. Nature (1992) 2.54
N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res (1979) 2.05
Peroxide sensors for the fission yeast stress-activated mitogen-activated protein kinase pathway. Mol Biol Cell (2001) 1.80
Changes in pneumococcal serotypes and antibiotypes carried by vaccinated and unvaccinated day-care centre attendees in Portugal, a country with widespread use of the seven-valent pneumococcal conjugate vaccine. Clin Microbiol Infect (2009) 1.79
A rabbit with the allotypic phenotype: ala2a3 b4b5b6. J Immunol (1974) 1.77
Reshaping a human antibody to inhibit the interleukin 6-dependent tumor cell growth. Cancer Res (1993) 1.77
Genetic diversity and clonal patterns among antibiotic-susceptible and -resistant Streptococcus pneumoniae colonizing children: day care centers as autonomous epidemiological units. J Clin Microbiol (2000) 1.64
Heterogeneity of cancer cells from a single human colon carcinoma. Am J Med (1981) 1.56
Identification of human genomic clones coding the major histocompatibility antigens HLA-a2 and HLA-B7 by DNA-mediated gene transfer. Proc Natl Acad Sci U S A (1982) 1.39
Carriage of respiratory tract pathogens and molecular epidemiology of Streptococcus pneumoniae colonization in healthy children attending day care centers in Lisbon, Portugal. Microb Drug Resist (1999) 1.38
Two variant surface glycoproteins of Trypanosoma brucei have a conserved C-terminus. Nature (1981) 1.35
The serum resistance-associated (SRA) gene of Trypanosoma brucei rhodesiense encodes a variant surface glycoprotein-like protein. Mol Biochem Parasitol (1994) 1.32
Camel heavy-chain antibodies: diverse germline V(H)H and specific mechanisms enlarge the antigen-binding repertoire. EMBO J (2000) 1.28
Structure and mechanism of a sub-family of enzymes related to N-acetylneuraminate lyase. J Mol Biol (1997) 1.28
A new procedure for examining Golgi impregnated neurons by light and electron microscopy. J Neurocytol (1977) 1.27
Amino acid sequences in the alpha 1 domain and not glycosylation are important in HLA-A2/beta 2-microglobulin association and cell surface expression. Mol Cell Biol (1987) 1.26
DNA-mediated gene transfer without carrier DNA. J Cell Biol (1981) 1.25
The epidermolytic toxins are serine proteases. FEBS Lett (1990) 1.20
A gene expressed only in serum-resistant variants of Trypanosoma brucei rhodesiense. Mol Biochem Parasitol (1989) 1.19
Gene activation and re-expression of a Trypanosoma brucei variant surface glycoprotein. EMBO J (1983) 1.19
Humanization of a mouse monoclonal antibody by CDR-grafting: the importance of framework residues on loop conformation. Protein Eng (1991) 1.18
Trends in drug resistance, serotypes, and molecular types of Streptococcus pneumoniae colonizing preschool-age children attending day care centers in Lisbon, Portugal: a summary of 4 years of annual surveillance. J Clin Microbiol (2005) 1.16
Natural history of drug-resistant clones of Streptococcus pneumoniae colonizing healthy children in Portugal. Microb Drug Resist (2005) 1.14
Is physiologic sympathoadrenal catecholamine release exocytotic in humans? Circulation (1990) 1.14
Three-dimensional structure of 2-amino-3-ketobutyrate CoA ligase from Escherichia coli complexed with a PLP-substrate intermediate: inferred reaction mechanism. Biochemistry (2001) 1.13
Only the serum-resistant bloodstream forms of Trypanosoma brucei rhodesiense express the serum resistance associated (SRA) protein. Ann Soc Belg Med Trop (1992) 1.12
Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J Immunol (1988) 1.12
Chromogranin A. Storage and release in hypertension. Hypertension (1990) 1.12
Analytical and clinical sensitivity of West Nile virus RNA screening and supplemental assays available in 2003. Transfusion (2005) 1.12
Recognition of HLA-A2 and -B7 antigens by cloned cytotoxic T lymphocytes after gene transfer into human and monkey, but not mouse, cells. Proc Natl Acad Sci U S A (1984) 1.11
Different mechanisms account for the suppression of interleukin 2 production and the suppression of interleukin 2 receptor expression in Trypanosoma brucei-infected mice. Eur J Immunol (1989) 1.10
Experimental Trypanosoma brucei infections selectively suppress both interleukin 2 production and interleukin 2 receptor expression. Eur J Immunol (1987) 1.10
The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia. Parasitology (1998) 1.09
Dual role of macrophages in the suppression of interleukin 2 production and interleukin 2 receptor expression in trypanosome-infected mice. Eur J Immunol (1989) 1.09
Somatic cell genetic analysis of HLA-A, B, C and human beta 2-microglobulin expression. Somatic Cell Genet (1982) 1.08
Evidence for kinetoplast and nuclear DNA homogeneity in Trypanosoma evansi isolates. Mol Biochem Parasitol (1990) 1.08
CTL adhesion and antigen recognition are discrete steps in the human CTL-target cell interaction. J Immunol (1987) 1.07
Catecholamine storage vesicle protein expression in genetic hypertension. Blood Press (1999) 1.07
Optimization of primers for cloning libraries of mouse immunoglobulin genes using the polymerase chain reaction. Eur J Immunol (1993) 1.05
T3 monoclonal antibody activation of nonspecific cytolysis: a mechanism of CTL inhibition. J Immunol (1985) 1.03
A card agglutination test (CATT) for veterinary use based on an early VAT RoTat 1/2 of Trypanosoma evansi. Ann Soc Belg Med Trop (1988) 1.02
The specific variable domain of camel heavy-chain antibodies is encoded in the germline. J Mol Biol (1998) 1.02
A role for TNF during African trypanosomiasis: involvement in parasite control, immunosuppression and pathology. Res Immunol (1993) 1.00
Humanization of a mouse antibody against human alpha-4 integrin: a potential therapeutic for the treatment of multiple sclerosis. Hum Antibodies (1997) 1.00
Loss of splice consensus signal is responsible for the removal of the entire C(H)1 domain of the functional camel IGG2A heavy-chain antibodies. Mol Immunol (1999) 0.99
Development of new cloning vectors for the production of immunogenic outer membrane fusion proteins in Escherichia coli. Biotechnology (N Y) (1996) 0.98
Infectivity of Trypanosoma (Trypanozoon) brucei gambiense for baboons (Papio hamadryas, Papio papio). Ann Soc Belg Med Trop (1991) 0.98
Murine tumour necrosis factor plays a protective role during the initial phase of the experimental infection with Trypanosoma brucei brucei. Parasite Immunol (1993) 0.98
An approach to the cloning of cell surface protein genes. Selection by cell sorting of mouse L-cells that express HLA or 4F2 antigens after transformation with total human DNA. Mol Biol Med (1983) 0.98
Structural studies on the PH domains of Db1, Sos1, IRS-1, and beta ARK1 and their differential binding to G beta gamma subunits. Biochemistry (1995) 0.96
Rabbit leukocyte surface antigens defined by monoclonal antibodies. Eur J Immunol (1983) 0.94
Species-restricted recognition of transfected HLA-A2 and HLA-B7 by human CTL clones. J Immunol (1986) 0.92
Trypanosoma (Nannomonas) congolense: identification of two karyotypic groups. EMBO J (1985) 0.92
Identification of virus-like particles in Eimeria stiedae. Mol Biochem Parasitol (1989) 0.92
Cytotoxic T lymphocytes from HLA-A2 transgenic mice specific for HLA-A2 expressed on human cells. J Exp Med (1988) 0.91
Nonrandom allele associations between unlinked protein loci: are the polymorphisms of the immunoglobulin constant regions adaptive? Proc Natl Acad Sci U S A (1987) 0.91
Blood donor screening with cobas s 201/cobas TaqScreen MPX under routine conditions at German Red Cross institutes. Vox Sang (2009) 0.90
Structural analysis of the functional sites of class I HLA antigens. Immunol Rev (1985) 0.90
Endoscopists' estimation of size should not determine surveillance of colonic polyps. Colorectal Dis (2009) 0.89
Efficacy and safety of omapatrilat with hydrochlorothiazide for the treatment of hypertension in subjects nonresponsive to hydrochlorothiazide alone. Am J Hypertens (2001) 0.89
Nephrotic syndrome in bronchogenic carcinoma: report of two cases with immunochemical studies. Clin Nephrol (1975) 0.88
Gene mapping and somatic cell hybrid analysis of the role of human lymphocyte function-associated antigen-3 (LFA-3) in CTL-target cell interactions. J Immunol (1986) 0.87
Inverted repeat structure and homologous sequences in the LD1 amplicons of Leishmania spp. Mol Biochem Parasitol (1994) 0.87
Camel trypanosomosis in Morocco. 2. A pilot disease control trial. Vet Parasitol (2003) 0.87
An improved method for preparing DNA from human brain. J Neurosci Methods (1984) 0.86
Differences in rearrangements of H1 and H5 in chicken erythrocyte chromatin. Biochemistry (1981) 0.86
Evidence for quasi-silent germline genes coding for phylogenetically ancient determinants of the rabbit a locus allotypes. Eur J Immunol (1977) 0.86
Multiple allosteric sites on muscarinic receptors. Life Sci (2001) 0.85
DNA recombination associated with short direct repeats in Leishmania mexicana M379. Mol Biochem Parasitol (1992) 0.85
Cross-linking of nucleosomal histones with monofunctional imidoesters. Nucleic Acids Res (1978) 0.85
Probing HLA-B7 conformational shifts induced by peptide-binding groove mutations and bound peptide with anti-HLA monoclonal antibodies. J Immunol (1996) 0.84
[Canine leishmaniasis in the Rif mountains (Moroccan Mediterranean coast): a seroepidemiological survey]. Parasite (2003) 0.84
Expression in Escherichia coli of the putative N-acetylneuraminate lyase gene (nanA) from Haemophilus influenzae: overproduction, purification, and crystallization. Protein Expr Purif (1998) 0.84
Chromogranin A: localization and stoichiometry in large dense core catecholamine storage vesicles from sympathetic nerve. Brain Res (1991) 0.84
Detection and identification of occult HBV in blood donors in Taiwan using a commercial, multiplex, multi-dye nucleic acid amplification technology screening test. Vox Sang (2013) 0.84
Evidence for widespread asymptomatic Trypanosoma rhodesiense human infection in the Luangwa Valley (Zambia). Trop Med Parasitol (1991) 0.84
Upon the observation of superbeads in chromatin. Nucleic Acids Res (1980) 0.84
Partial control of hepatitis delta virus superinfection by immunisation of woodchucks (Marmota monax) with hepatitis delta antigen expressed by a recombinant vaccinia or baculovirus. J Med Virol (1993) 0.83
Suppression of chromogranin-A release from neuroendocrine sources in man: pharmacological studies. J Clin Endocrinol Metab (1991) 0.83
Cloning and analysis of Trypanosoma (Nannomonas) congolense ILNat 2.1 VSG gene. Mol Biochem Parasitol (1985) 0.83
Rabbit heavy chain haplotypes--allotypic determinants expressed by VH-CH recombinants. Immunogenetics (1982) 0.83
Rheumatoid factor appearance in Micrococcus lysodeikticus immunization and its interference with allotype specific reactions. Z Immunitatsforsch Exp Klin Immunol (1975) 0.83
Bacterial orthologues indicate the malarial plastid gene ycf24 is essential. Protist (2000) 0.83
Homologous peptides from the Fc region of rabbit IgG. Immunochemistry (1973) 0.82
Humanization of a mouse anti-human interleukin-6 receptor antibody comparing two methods for selecting human framework regions. Mol Immunol (1994) 0.82
Chromogranin A epitopes: clues from synthetic peptides and peptide mapping. Neuropeptides (1992) 0.82
Double line phenotypes in rabbit IgG allotypes and their relation to the cis/trans configuration of the IgG markers. J Immunogenet (1977) 0.82
Mutational analysis of two DR alpha residues involved in dimers of HLA-DR molecules. J Immunol (1995) 0.82
Mechanisms underlying trypanosome-elicited immunosuppression. Ann Soc Belg Med Trop (1992) 0.82
Plasticity of gp63 gene organization in Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana. Parasitology (1995) 0.82
Polymerase chain reaction-mediated cloning and in vitro translation of the genes coding for the structural proteins of hog cholera virus. Arch Virol (1993) 0.81
Evidence for genetic diversity in Trypanosoma (Nannomonas) congolense. Parasitology (1986) 0.81