A rapid optimized protocol for downward alkaline Southern blotting of DNA.

PubWeight™: 1.38‹?› | Rank: Top 10%

🔗 View Article (PMID 8373591)

Published in Biotechniques on August 01, 1993

Authors

P A Koetsier1, J Schorr, W Doerfler

Author Affiliations

1: Institute for Genetics, University of Cologne, FRG.

Articles citing this

Defects in nuclear structure and function promote dilated cardiomyopathy in lamin A/C-deficient mice. J Clin Invest (2004) 2.67

Identification of genes involved in DNA replication of the Autographa californica baculovirus. Proc Natl Acad Sci U S A (1994) 2.48

Significance of the expression of the CER6 condensing enzyme for cuticular wax production in Arabidopsis. Plant Physiol (2002) 1.73

Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA. Proc Natl Acad Sci U S A (1997) 1.43

Chromosomal insertion of foreign (adenovirus type 12, plasmid, or bacteriophage lambda) DNA is associated with enhanced methylation of cellular DNA segments. Proc Natl Acad Sci U S A (1995) 1.15

Insertion of foreign DNA into an established mammalian genome can alter the methylation of cellular DNA sequences. J Virol (1999) 1.14

Mutational analysis of the 18-base-pair inverted repeat element at the bovine papillomavirus origin of replication: identification of critical sequences for E1 binding and in vivo replication. J Virol (1995) 1.14

Norfloxacin-induced DNA gyrase cleavage complexes block Escherichia coli replication forks, causing double-stranded breaks in vivo. Mol Microbiol (2005) 1.11

A core subunit of the RNA-processing/degrading exosome specifically influences cuticular wax biosynthesis in Arabidopsis. Plant Cell (2007) 1.08

Two immediate-early pathogen-responsive members of the AtCMPG gene family in Arabidopsis thaliana and the W-box-containing elicitor-response element of AtCMPG1. Proc Natl Acad Sci U S A (2002) 1.04

Transgenic tobacco plants overexpressing the heterologous lea gene Rab16A from rice during high salt and water deficit display enhanced tolerance to salinity stress. Plant Cell Rep (2007) 1.04

Comparative physiological and molecular responses of a common aromatic indica rice cultivar to high salinity with non-aromatic indica rice cultivars. Plant Cell Rep (2008) 1.00

The initiation of de novo methylation of foreign DNA integrated into a mammalian genome is not exclusively targeted by nucleotide sequence. J Virol (1995) 0.94

Methylation mosaicism of 5'-(CGG)(n)-3' repeats in fragile X, premutation and normal individuals. Nucleic Acids Res (2000) 0.91

Down-regulation of Fas gene expression in colon cancer is not a result of allelic loss or gene rearrangement. Br J Cancer (1998) 0.90

Posttranscriptional gene silencing in transgenic sugarcane. Dissection Of homology-dependent virus resistance in a monocot that has a complex polyploid genome Plant Physiol (1999) 0.89

Characterization of the interaction between P143 and LEF-3 from two different baculovirus species: Choristoneura fumiferana nucleopolyhedrovirus LEF-3 can complement Autographa californica nucleopolyhedrovirus LEF-3 in supporting DNA replication. J Virol (2004) 0.89

The structure of adenovirus type 12 DNA integration sites in the hamster cell genome. J Virol (1996) 0.89

Selective sites of adenovirus (foreign) DNA integration into the hamster genome: changes in integration patterns. J Virol (1994) 0.88

Influence of in vitro manipulation on the stability of methylation patterns in the Snurf/Snrpn-imprinting region in mouse embryonic stem cells. Nucleic Acids Res (2004) 0.88

Deletion of the Schizophyllum commune A alpha locus: the roles of A alpha Y and Z mating-type genes. Genetics (1996) 0.86

Late transcripts of adenovirus type 12 DNA are not translated in hamster cells expressing the E1 region of adenovirus type 5. J Virol (1994) 0.83

Epigenetic status of an adenovirus type 12 transgenome upon long-term cultivation in hamster cells. J Virol (2007) 0.82

Overexpression of the adenovirus type 12 (Ad12) pTP or E1A gene facilitates Ad12 DNA replication in nonpermissive BHK21 hamster cells. J Virol (2001) 0.81

Patterns of frog virus 3 DNA methylation and DNA methyltransferase activity in nuclei of infected cells. J Virol (1993) 0.81

The gastrointestinal tract as the portal of entry for foreign macromolecules: fate of DNA and proteins. Mol Genet Genomics (2003) 0.80

A fully 5'-CG-3' but not a 5'-CCGG-3' methylated late frog virus 3 promoter retains activity. J Virol (1995) 0.76

Broad AOX expression in a genetically tractable mouse model does not disturb normal physiology. Dis Model Mech (2016) 0.75

Articles by these authors

(truncated to the top 100)

DNA methylation and gene activity. Annu Rev Biochem (1983) 9.23

Nonproductive infection of baby hamster kidney cells (BHK21) with adenovirus type 12. Virology (1969) 4.23

Methylation of integrated adenovirus type 12 DNA sequences in transformed cells is inversely correlated with viral gene expression. Proc Natl Acad Sci U S A (1980) 3.89

Denaturation pattern of the DNA of adenovirus type 2 as determined by electron microscopy. J Mol Biol (1970) 3.35

Structure and composition of the adenovirus type 2 core. J Virol (1975) 3.21

CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen. J Immunol (1998) 2.93

Patterns of integration of viral DNA sequences in the genomes of adenovirus type 12-transformed hamster cells. Cell (1978) 2.82

Infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus I. Synthesis of intracellular proteins after virus infection. Virology (1979) 2.70

Establishment of de novo DNA methylation patterns. Transcription factor binding and deoxycytidine methylation at CpG and non-CpG sequences in an integrated adenovirus promoter. J Mol Biol (1990) 2.69

DNA methylation in the Alu sequences of diploid and haploid primary human cells. EMBO J (1993) 2.47

Why is CpG suppressed in the genomes of virtually all small eukaryotic viruses but not in those of large eukaryotic viruses? J Virol (1994) 2.45

Infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus II. The viral DNA and the kinetics of its replication. Virology (1979) 2.42

Expression of a cloned adenovirus gene is inhibited by in vitro methylation. Proc Natl Acad Sci U S A (1982) 2.23

DNA methylation--a regulatory signal in eukaryotic gene expression. J Gen Virol (1981) 2.19

DNA methylation in adenovirus, adenovirus-transformed cells, and host cells. Proc Natl Acad Sci U S A (1976) 2.17

Integration of the deoxyribonucleic acid of adenovirus type 12 into the deoxyribonucleic acid of baby hamster kidney cells. J Virol (1970) 2.17

The fate of the DNA of adenovirus type 12 in baby hamster kidney cells. Proc Natl Acad Sci U S A (1968) 2.13

Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. J Virol (1976) 2.12

Interindividual concordance of methylation profiles in human genes for tumor necrosis factors alpha and beta. Proc Natl Acad Sci U S A (1990) 2.03

DNA methylation and viral gene expression in adenovirus-transformed and -infected cells. Nucleic Acids Res (1980) 2.00

Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector. EMBO J (1986) 1.98

Trans effect of the E1 region of adenoviruses on the expression of a prokaryotic gene in mammalian cells: resistance to 5' -CCGG- 3' methylation. Proc Natl Acad Sci U S A (1986) 1.92

A single-tube PCR test for the diagnosis of Angelman and Prader-Willi syndrome based on allelic methylation differences at the SNRPN locus. Eur J Hum Genet (1997) 1.90

The position and orientation of genes in lambda and lambda dg DNA. Cold Spring Harb Symp Quant Biol (1966) 1.87

Integration sites of adenovirus type 12 DNA in transformed hamster cells and hamster tumor cells. J Virol (1980) 1.79

Transcription of overlapping sets of RNAs from the genome of Autographa californica nuclear polyhedrosis virus: a novel method for mapping RNAs. J Virol (1984) 1.78

Expression of the chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters: effect of promoter methylation on gene expression. Proc Natl Acad Sci U S A (1983) 1.76

Incomplete particles of adenovirus. II. Kinetics of formation and polypeptide composition of adenovirus type 2. Virology (1974) 1.74

Adenovirus L1 52- and 55-kilodalton proteins are required for assembly of virions. J Virol (1989) 1.71

Mapping of Early and Late Transcripts Encoded by the Autographa californica Nuclear Polyhedrosis Virus Genome: Is Viral RNA Spliced? J Virol (1984) 1.71

Intracellular forms of adenovirus deoxyribonucleic acid. I. Evidence for a deoxyribonucleic acid-protein complex in baby hamster kidney cells infected with adenovirus type 12. J Virol (1972) 1.68

Genomic sequencing reveals a 5-methylcytosine-free domain in active promoters and the spreading of preimposed methylation patterns. Proc Natl Acad Sci U S A (1989) 1.65

Three size-classes of intracellular adenovirus deoxyribonucleic acid. J Virol (1971) 1.65

Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis. J Virol (1994) 1.61

The role of CpG dinucleotides in DNA vaccines. Trends Microbiol (1998) 1.57

Imprinted segments in the human genome: different DNA methylation patterns in the Prader-Willi/Angelman syndrome region as determined by the genomic sequencing method. Hum Mol Genet (1997) 1.55

Autographa californica nuclear polyhedrosis virus (AcNPV) DNA does not persist in mass cultures of mammalian cells. Virology (1983) 1.50

DNA methylation of three 5' C-C-G-G 3' sites in the promoter and 5' region inactivate the E2a gene of adenovirus type 2. Proc Natl Acad Sci U S A (1984) 1.50

Intracellular forms of adenovirus DNA. 3. Integration of the DNA of adenovirus type 2 into host DNA in productively infected cells. J Virol (1974) 1.48

Adenovirus endonuclease: association with the penton of adenovirus type 2. J Mol Biol (1971) 1.45

Overlapping sets of viral RNAs reflect the array of polypeptides in the EcoRI J and N fragments (map positions 81.2 to 85.0) of the Autographa californica nuclear polyhedrosis virus genome. J Virol (1987) 1.44

Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA. Proc Natl Acad Sci U S A (1997) 1.43

Induction of transcription factor AP-1 by adenovirus E1A protein and cAMP. Genes Dev (1989) 1.42

Expression of viral DNA in adenovirus type 12-transformed cells, in tumor cells, and in revertants. J Virol (1981) 1.41

Uptake and fate of the DNA of adenovirus type 2 in KB cells. Virology (1975) 1.39

Structure of the anchor-domain of myristoylated and non-myristoylated HIV-1 Nef protein. J Mol Biol (1999) 1.39

Uptake, fixation, and expression of foreign DNA in mammalian cells: the organization of integrated adenovirus DNA sequences. Curr Top Microbiol Immunol (1982) 1.39

Reactivation of the methylation-inactivated late E2A promoter of adenovirus type 2 by E1A (13 S) functions. J Mol Biol (1988) 1.38

Temperature-sensitive mutants of human adenovirus type 12. Virology (1971) 1.38

The unmethylated state of the promoter/leader and 5'-regions of integrated adenovirus genes correlates with gene expression. EMBO J (1982) 1.37

DNA methylation and its functional significance: studies on the adenovirus system. Curr Top Microbiol Immunol (1984) 1.37

Incomplete particles of adenovirus. I. Characteristics of the DNA associated with incomplete adenovirions of types 2 and 12. Virology (1974) 1.37

Intracellular forms of adenovirus DNA. V. Viral DNA sequences in hamster cells abortively infected and transformed with human adenovirus type 12. J Virol (1976) 1.36

Selectivity in integration sites of adenoviral DNA. Cold Spring Harb Symp Quant Biol (1980) 1.35

Site of linkage between adenovirus type 12 and cell DNAs in hamster tumour line CLAC3. Nature (1981) 1.34

The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector. Virology (1990) 1.34

An unusual symmetric recombinant between adenovirus type 12 DNA and human cell DNA. Proc Natl Acad Sci U S A (1981) 1.33

Patterns of integration of viral DNA in adenovirus type 2-transformed hamster cells. J Mol Biol (1981) 1.32

Tumor induction by human adenovirus type 12 in hamsters: loss of the viral genome from adenovirus type 12-induced tumor cells is compatible with tumor formation. EMBO J (1982) 1.31

Methylation analysis of the PWS/AS region does not support an enhancer-competition model. Nat Genet (1998) 1.30

The translational map of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome. EMBO J (1982) 1.28

Neurological phenotype in Waardenburg syndrome type 4 correlates with novel SOX10 truncating mutations and expression in developing brain. Am J Hum Genet (2000) 1.26

Transcription of the genome of adenovirus type 12. I. Viral mRNA in abortively infected and transformed cells. J Virol (1975) 1.26

Recombination in hamster cell nuclear extracts between adenovirus type 12 DNA and two hamster preinsertion sequences. EMBO J (1989) 1.25

Shifts in the extent and patterns of DNA methylation upon explanation and subcultivation of adenovirus type 12-induced hamster tumor cells. Virology (1982) 1.25

Morphological revertants of adenovirus type 12-transformed hamster cells. J Gen Virol (1978) 1.23

Transcriptional activities of mammalian genomes at sites of recombination with foreign DNA. J Virol (1987) 1.23

Intracellular forms of adenovirus DNA: integrated form of adenovirus DNA appears early in productive infection. Proc Natl Acad Sci U S A (1976) 1.22

An endonuclease in cells infected with adenovirus and associated with adenovirions. Virology (1972) 1.21

On the mechanism of recombination between adenoviral and cellular DNAs: the structure of junction sites. Curr Top Microbiol Immunol (1984) 1.20

The DNA of adenovirus type 12 and its denaturation pattern. Virology (1972) 1.20

Nucleotide sequence at the site of junction between adenovirus type 12 DNA and repetitive hamster cell DNA in transformed cell line CLAC1. Nucleic Acids Res (1982) 1.20

Gene orientation in bacteriophage lambda as determined from the genetic activities of heteroduplex DNA formed in vitro. J Mol Biol (1968) 1.19

Adenovirus type 12-induced rat tumor cells of neuroepithelial origin: persistence and expression of the viral genome. J Virol (1980) 1.19

Epigenetic and genotype-specific effects on the stability of de novo imposed methylation patterns in transgenic mice. J Biol Chem (2000) 1.17

Integration of viral DNA into the genome of the adenovirus type 2-transformed hamster cell line HE5 without loss or alteration of cellular nucleotides. Nucleic Acids Res (1983) 1.17

Methylation of integrated viral DNA sequences in hamster cells transformed by adenovirus 12. Cold Spring Harb Symp Quant Biol (1980) 1.16

Absence of replication of the DNA of adenovirus type 12 in BHK21 cells. Virology (1970) 1.16

Chromosomal insertion of foreign (adenovirus type 12, plasmid, or bacteriophage lambda) DNA is associated with enhanced methylation of cellular DNA segments. Proc Natl Acad Sci U S A (1995) 1.15

Insertion of foreign DNA into an established mammalian genome can alter the methylation of cellular DNA sequences. J Virol (1999) 1.14

Spreading of DNA methylation across integrated foreign (adenovirus type 12) genomes in mammalian cells. J Virol (1991) 1.14

The cloned EcoRI fragments of Autographa californica nuclear polyhedrosis virus DNA. Gene (1981) 1.12

Loss of viral genomes from hamster tumor cells and nonrandom alterations in patterns of methylation of integrated adenovirus type 12 DNA. J Virol (1983) 1.11

Patch homologies and the integration of adenovirus DNA in mammalian cells. EMBO J (1982) 1.11

Intracellular forms of Adenovirus DNA. II. Isolation in dye-buoyant density gradients of a DNA-RNA complex from KB cells infected with Adenovirus type 2. J Virol (1973) 1.11

In vitro translation of adenovirus type 12-specific mRNA isolated from infected and transformed cells. J Virol (1979) 1.10

The promoter of the late p10 gene in the insect nuclear polyhedrosis virus Autographa californica: activation by viral gene products and sensitivity to DNA methylation. EMBO J (1985) 1.10

Cloned fragments of human adenovirus type-12 DNA. Gene (1981) 1.10

On the fate of orally ingested foreign DNA in mice: chromosomal association and placental transmission to the fetus. Mol Gen Genet (1998) 1.09

Revertants of adenovirus type 12-transformed hamster cell line T637 as tools in the analysis of integration patterns. J Virol (1980) 1.07

Ingested foreign (phage M13) DNA survives transiently in the gastrointestinal tract and enters the bloodstream of mice. Mol Gen Genet (1994) 1.06

Adenovirus type 2 VAI RNA transcription by polymerase III is blocked by sequence-specific methylation. J Virol (1991) 1.06

Sequence-specific methylation in a downstream region of the late E2A promoter of adenovirus type 2 DNA prevents protein binding. J Mol Biol (1989) 1.06

Quantitative determination of 5-methylcytosine in DNA by reverse-phase high-performance liquid chromatography. Anal Biochem (1983) 1.06

Methylation of adenovirus genes in transformed cells and in vitro: influence on the regulation of gene expression? Eur J Cell Biol (1981) 1.06

Rapid protein sequencing by tandem mass spectrometry and cDNA cloning of p20-CGGBP. A novel protein that binds to the unstable triplet repeat 5'-d(CGG)n-3' in the human FMR1 gene. J Biol Chem (1997) 1.05

Foreign DNA integration. Genome-wide perturbations of methylation and transcription in the recipient genomes. J Biol Chem (2001) 1.05

Incomplete particles of adenovirus type 2. III. Viral and cellular DNA sequences in incomplete particles. Virology (1977) 1.05

Cognitive and behavioral profile of fragile X boys: correlations to molecular data. Am J Med Genet (2000) 1.03

Integration of adenovirus DNA into the cellular genome. Cold Spring Harb Symp Quant Biol (1975) 1.03