Expression and purification of mammalian Sarl.

PubWeight™: 1.12‹?› | Rank: Top 10%

🔗 View Article (PMID 8583938)

Published in Methods Enzymol on January 01, 1995

Authors

T Rowe1, W E Balch

Author Affiliations

1: Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.

Articles citing this

Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport. J Cell Biol (1995) 3.57

Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering. J Cell Biol (1998) 3.31

An ordered inheritance strategy for the Golgi apparatus: visualization of mitotic disassembly reveals a role for the mitotic spindle. J Cell Biol (1998) 2.52

The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly. J Cell Biol (2001) 2.41

Localization and recycling of gp27 (hp24gamma3): complex formation with other p24 family members. Mol Biol Cell (1999) 1.60

Endoplasmic reticulum export of glycosyltransferases depends on interaction of a cytoplasmic dibasic motif with Sar1. Mol Biol Cell (2003) 1.41

Activation of phospholipase D by the small GTPase Sar1p is required to support COPII assembly and ER export. EMBO J (2003) 1.31

Sar1 assembly regulates membrane constriction and ER export. J Cell Biol (2010) 1.26

Targeting sequences of UBXD8 and AAM-B reveal that the ER has a direct role in the emergence and regression of lipid droplets. J Cell Sci (2009) 1.20

Spatial separation of Golgi and ER during mitosis protects SREBP from unregulated activation. EMBO J (2008) 1.19

A cascade of ER exit site assembly that is regulated by p125A and lipid signals. J Cell Sci (2014) 0.78

Geranylgeranyl-regulated transport of the prenyltransferase UBIAD1 between membranes of the ER and Golgi. J Lipid Res (2016) 0.75

Articles by these authors

(truncated to the top 100)

Methanogens: reevaluation of a unique biological group. Microbiol Rev (1979) 29.84

The phylogeny of prokaryotes. Science (1980) 24.57

New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol (1976) 15.95

Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine. Cell (1984) 10.74

Classification of methanogenic bacteria by 16S ribosomal RNA characterization. Proc Natl Acad Sci U S A (1977) 5.09

Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science (2000) 4.56

Semi-intact cells permeable to macromolecules: use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex. Cell (1987) 4.39

GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex. J Cell Biol (1992) 4.34

Role for adenosine triphosphate in regulating the assembly and transport of vesicular stomatitis virus G protein trimers. J Cell Biol (1987) 3.84

Dominant inhibitory mutants of ARF1 block endoplasmic reticulum to Golgi transport and trigger disassembly of the Golgi apparatus. J Biol Chem (1994) 3.71

Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein. Nature (1989) 3.62

Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport. J Cell Biol (1995) 3.57

Characterization of protein transport between successive compartments of the Golgi apparatus: asymmetric properties of donor and acceptor activities in a cell-free system. Arch Biochem Biophys (1985) 3.43

The organization of endoplasmic reticulum export complexes. J Cell Biol (1996) 3.32

Mutations in GDI1 are responsible for X-linked non-specific mental retardation. Nat Genet (1998) 3.30

Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus. J Cell Biol (1989) 3.26

Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. J Cell Biol (1991) 3.22

Sequential intermediates in the pathway of intercompartmental transport in a cell-free system. Cell (1984) 3.07

A di-acidic signal required for selective export from the endoplasmic reticulum. Science (1997) 3.05

Specificity and biological distribution of coenzyme M (2-mercaptoethanesulfonic acid). J Bacteriol (1979) 2.95

Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments. J Cell Biol (1994) 2.75

Role for phosphatidylinositol 3-kinase in the sorting and transport of newly synthesized lysosomal enzymes in mammalian cells. J Cell Biol (1995) 2.74

Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering. Traffic (2001) 2.53

ATP-coupled transport of vesicular stomatitis virus G protein between the endoplasmic reticulum and the Golgi. J Biol Chem (1986) 2.45

Morphological analysis of protein transport from the ER to Golgi membranes in digitonin-permeabilized cells: role of the P58 containing compartment. J Cell Biol (1992) 2.44

An ancient divergence among the bacteria. J Mol Evol (1977) 2.44

GTPases: multifunctional molecular switches regulating vesicular traffic. Annu Rev Biochem (1994) 2.44

Cargo selection by the COPII budding machinery during export from the ER. J Cell Biol (1998) 2.44

The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly. J Cell Biol (2001) 2.41

Integration of endoplasmic reticulum signaling in health and disease. Nat Med (1999) 2.35

Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium. J Bacteriol (1979) 2.28

Quality control in the endoplasmic reticulum: folding and misfolding of vesicular stomatitis virus G protein in cells and in vitro. J Cell Biol (1990) 2.22

A GDP-bound of rab1 inhibits protein export from the endoplasmic reticulum and transport between Golgi compartments. J Cell Biol (1994) 2.21

COPII vesicles derived from mammalian endoplasmic reticulum microsomes recruit COPI. J Cell Biol (1996) 2.11

Isoprenoid modification of rab proteins terminating in CC or CXC motifs. Proc Natl Acad Sci U S A (1991) 1.99

Membrane dynamics at the endoplasmic reticulum-Golgi interface. J Cell Biol (1997) 1.96

A Rab1 mutant affecting guanine nucleotide exchange promotes disassembly of the Golgi apparatus. J Cell Biol (1994) 1.91

ATP-coupled transport of vesicular stomatitis virus G protein. Functional boundaries of secretory compartments. J Biol Chem (1986) 1.89

Beta-COP is essential for transport of protein from the endoplasmic reticulum to the Golgi in vitro. J Cell Biol (1993) 1.88

Rab1 and Ca2+ are required for the fusion of carrier vesicles mediating endoplasmic reticulum to Golgi transport. J Cell Biol (1994) 1.83

Synthetic peptides of the Rab effector domain inhibit vesicular transport through the secretory pathway. EMBO J (1990) 1.71

A novel prefusion complex formed during protein transport between Golgi cisternae in a cell-free system. J Biol Chem (1986) 1.68

Coenzyme M: preparation and assay. Methods Enzymol (1980) 1.66

Syntaxin 5 regulates endoplasmic reticulum to Golgi transport. J Biol Chem (1994) 1.63

Differential inhibition of multiple vesicular transport steps between the endoplasmic reticulum and trans Golgi network. J Biol Chem (1993) 1.63

Kinase signaling initiates coat complex II (COPII) recruitment and export from the mammalian endoplasmic reticulum. J Biol Chem (2000) 1.59

Structure and mutational analysis of Rab GDP-dissociation inhibitor. Nature (1996) 1.56

A di-acidic (DXE) code directs concentration of cargo during export from the endoplasmic reticulum. J Biol Chem (1999) 1.56

Multiple GTP-binding proteins regulate vesicular transport from the ER to Golgi membranes. J Cell Biol (1992) 1.52

Sequential transport of protein between the endoplasmic reticulum and successive Golgi compartments in semi-intact cells. J Biol Chem (1991) 1.52

Furin initiates gelsolin familial amyloidosis in the Golgi through a defect in Ca(2+) stabilization. EMBO J (2001) 1.52

Rab2 is essential for the maturation of pre-Golgi intermediates. J Biol Chem (1996) 1.52

Role of vesicle-associated syntaxin 5 in the assembly of pre-Golgi intermediates. Science (1998) 1.50

Evidence for the regulation of exocytic transport by protein phosphorylation. J Cell Biol (1992) 1.48

Crystal structure of Sar1-GDP at 1.7 A resolution and the role of the NH2 terminus in ER export. J Cell Biol (2001) 1.47

Cargo can modulate COPII vesicle formation from the endoplasmic reticulum. J Biol Chem (1999) 1.47

Reconstitution of transport of vesicular stomatitis virus G protein from the endoplasmic reticulum to the Golgi complex using a cell-free system. J Cell Biol (1987) 1.47

Getting into the Golgi. Trends Cell Biol (1998) 1.44

AP-2-containing clathrin coats assemble on mature lysosomes. J Cell Biol (1996) 1.43

Exocytotic fusion is activated by Rab3a peptides. Nature (1992) 1.41

Guanine nucleotide dissociation inhibitor is essential for Rab1 function in budding from the endoplasmic reticulum and transport through the Golgi stack. J Cell Biol (1994) 1.37

The mammalian guanine nucleotide exchange factor mSec12 is essential for activation of the Sar1 GTPase directing endoplasmic reticulum export. Traffic (2001) 1.35

Principles of selective transport: coat complexes hold the key. Trends Cell Biol (1996) 1.34

p53/58 binds COPI and is required for selective transport through the early secretory pathway. J Cell Biol (1997) 1.33

Ricin cytotoxicity is sensitive to recycling between the endoplasmic reticulum and the Golgi complex. J Biol Chem (1995) 1.30

A synthetic peptide of the rab3a effector domain stimulates amylase release from permeabilized pancreatic acini. Proc Natl Acad Sci U S A (1992) 1.29

The glycoprotein that is transported between successive compartments of the Golgi in a cell-free system resides in stacks of cisternae. Cell (1984) 1.29

Structural insights into the function of the Rab GDI superfamily. Trends Biochem Sci (1996) 1.28

Phytanyl-glycerol ethers and squalenes in the archaebacterium Methanobacterium thermoautotrophicum. J Mol Evol (1978) 1.28

Sequence homologies in the N-terminal region of the ribosomal 'A' proteins from Methanobacterium Thermoautotrophicum and Halobacterium cutirubrum. Biochim Biophys Acta (1980) 1.26

Organization of the Rab-GDI/CHM superfamily: the functional basis for choroideremia disease. Traffic (2001) 1.25

Protein sorting by directed maturation of Golgi compartments. Science (1999) 1.23

Mammalian Sly1 regulates syntaxin 5 function in endoplasmic reticulum to Golgi transport. J Biol Chem (1996) 1.22

Sequential intermediates in the transport of protein between the endoplasmic reticulum and the Golgi. J Biol Chem (1990) 1.21

Differential regulation of exocytosis by calcium and CAPS in semi-intact synaptosomes. Neuron (1998) 1.20

Mss4 does not function as an exchange factor for Rab in endoplasmic reticulum to Golgi transport. Mol Biol Cell (1997) 1.13

Stimulation of insulin release from permeabilized HIT-T15 cells by a synthetic peptide corresponding to the effector domain of the small GTP-binding protein rab3. FEBS Lett (1993) 1.08

Synthetic peptides of the effector-binding domain of rab enhance secretion from digitonin-permeabilized chromaffin cells. FEBS Lett (1992) 1.04

Traffic pattern of cystic fibrosis transmembrane regulator through the early exocytic pathway. Traffic (2000) 1.02

Molecular basis for Rab prenylation. J Cell Biol (2000) 1.00

A Rab GTPase is required for homotypic assembly of the endoplasmic reticulum. J Biol Chem (1997) 0.97

Molecular dissection of guanine nucleotide dissociation inhibitor function in vivo. Rab-independent binding to membranes and role of Rab recycling factors. J Biol Chem (1999) 0.96

Selective transport of cargo between the endoplasmic reticulum and Golgi compartments. Histochem Cell Biol (1998) 0.94

Export of protein from the endoplasmic reticulum is regulated by a diacylglycerol/phorbol ester binding protein. J Biol Chem (1994) 0.93

Membrane fusion: timing is everything. Nature (1996) 0.92

A new functional domain of guanine nucleotide dissociation inhibitor (alpha-GDI) involved in Rab recycling. Traffic (2000) 0.90

Transport-coupled oligosaccharide processing in a cell-free system. Methods Enzymol (1983) 0.90

Expression of wild-type and mutant green fluorescent protein-Rab1 for fluorescence microscopy analysis. Methods Enzymol (2001) 0.88

Molecular role for the Rab binding platform of guanine nucleotide dissociation inhibitor in endoplasmic reticulum to Golgi transport. J Biol Chem (1998) 0.85

Proteostasis, an emerging therapeutic paradigm for managing inflammatory airway stress disease. Curr Mol Med (2012) 0.85

Stage-specific assays to study biosynthetic cargo selection and role of SNAREs in export from the endoplasmic reticulum and delivery to the Golgi. Methods (2000) 0.84

Purification of His6-tagged Rab1 proteins using bacterial and insect cell expression systems. Methods Enzymol (1995) 0.83

A new frontier in pharmacology: the endoplasmic reticulum as a regulated export pathway in health and disease. Expert Opin Ther Targets (2001) 0.83

Transport of protein between endoplasmic reticulum and Golgi compartments in semiintact cells. Methods Enzymol (1992) 0.82

Use of two-stage incubations to define sequential intermediates in endoplasmic reticulum to Golgi transport. Methods Enzymol (1992) 0.82

Transient expression of small GTPases to study protein transport along secretory pathway in vivo using recombinant T7 vaccinia virus system. Methods Enzymol (1995) 0.81

Structural basis for Rab function: an overview. Methods Enzymol (2001) 0.80

Membrane fusion. Bridging the gap by AAA ATPases. Nature (1997) 0.80

Purification of properties of dihydroorotase, a zinc-containing metalloenzyme in Clostridium oroticum. J Bacteriol (1976) 0.80

Preparation of semiintact Chinese hamster ovary cells for reconstitution of endoplasmic reticulum-to-Golgi transport in a cell-free system. Methods Cell Biol (1989) 0.79