Published in Electrophoresis on June 01, 1996
Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching. Chem Rev (2012) 1.42
Molecular stretching of long DNA in agarose gel using alternating current electric fields. Biophys J (2002) 0.81
A MEMS differential viscometric sensor for affinity glucose detection in continuous glucose monitoring. J Micromech Microeng (2013) 0.81
Continuous monitoring of glucose in subcutaneous tissue using microfabricated differential affinity sensors. J Diabetes Sci Technol (2012) 0.80
Hydrophobically modified polyacrylamide block copolymers for fast, high-resolution DNA sequencing in microfluidic chips. Electrophoresis (2008) 0.76
Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol (1990) 61.47
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol (1986) 55.34
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol (1977) 33.84
Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A (1986) 32.58
Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol (1973) 30.17
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J Mol Biol (1983) 19.98
Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene (1987) 17.98
Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A (1984) 13.68
Bacteriophage T7. Science (1972) 13.19
Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proc Natl Acad Sci U S A (1986) 8.59
The genetics and physiology of bacteriophage T7. Virology (1969) 7.72
Utilization of bacteriophage T7 late promoters in recombinant plasmids during infection. J Mol Biol (1981) 5.66
Structural genomics: beyond the human genome project. Nat Genet (1999) 5.58
A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein. Cell (1986) 5.53
Inactivation of human immunodeficiency virus type 1 infectivity with preservation of conformational and functional integrity of virion surface proteins. J Virol (1998) 5.05
Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4. J Mol Biol (1981) 4.56
T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease 3. Proc Natl Acad Sci U S A (1973) 4.34
Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proc Natl Acad Sci U S A (1978) 4.01
T7 early RNAs are generated by site-specific cleavages. Proc Natl Acad Sci U S A (1973) 3.83
Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase. J Mol Biol (1984) 3.79
Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J Mol Biol (1975) 3.62
T7-directed protein synthesis. Virology (1969) 3.32
Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications. Nucleic Acids Res (1992) 3.28
The effect of dextran on microvascular thrombosis in an experimental rabbit model. Plast Reconstr Surg (1993) 3.12
Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein. Proc Natl Acad Sci U S A (1978) 3.11
Genetic analysis of non-essential bacteriophage T7 genes. J Mol Biol (1973) 2.88
Physical mapping of the early region of bacteriophage T7 DNA. J Mol Biol (1973) 2.74
T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell (1987) 2.66
Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J Mol Biol (1991) 2.66
Molecular substructure of a viral receptor-recognition protein. The gp17 tail-fiber of bacteriophage T7. J Mol Biol (1988) 2.59
Genetic and physical mapping in the early region of bacteriophage T7 DNA. J Mol Biol (1979) 2.52
Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli. J Bacteriol (1975) 2.50
A complete mapping of the proteins in the small ribosomal subunit of Escherichia coli. Science (1987) 2.43
The structural gene for a T7 endonuclease essential for phage DNA synthesis. Proc Natl Acad Sci U S A (1970) 2.40
Organization and expression of bacteriophage T7 DNA. Cold Spring Harb Symp Quant Biol (1983) 2.35
Containment of simian immunodeficiency virus infection: cellular immune responses and protection from rechallenge following transient postinoculation antiretroviral treatment. J Virol (2000) 2.24
Genetic and physical mapping of the late region of bacteriophage T7 DNA by use of cloned fragments of T7 DNA. J Mol Biol (1981) 2.20
Effects of consecutive AGG codons on translation in Escherichia coli, demonstrated with a versatile codon test system. J Bacteriol (1993) 2.05
Effects of the conformation of single-stranded DNA on renaturation and aggregation. J Mol Biol (1969) 2.00
Effect of RNAase III, cleavage on translation of bacteriophage T7 messenger RNAs. J Mol Biol (1975) 1.88
Gene 3 endonuclease of bacteriophage T7 resolves conformationally branched structures in double-stranded DNA. J Mol Biol (1987) 1.87
DNA sequencing by primer walking with strings of contiguous hexamers. Science (1992) 1.85
Conformational changes of single-stranded DNA. J Mol Biol (1969) 1.84
Signal-mediated import of bacteriophage T7 RNA polymerase into the Saccharomyces cerevisiae nucleus and specific transcription of target genes. Mol Cell Biol (1990) 1.84
Enzymes and sites of genetic recombination: studies with gene-3 endonuclease of phage T7 and with site-affinity mutants of phage lambda. Cold Spring Harb Symp Quant Biol (1984) 1.84
SAMase gene of bacteriophage T3 is responsible for overcoming host restriction. J Virol (1976) 1.82
The transcription termination site at the end of the early region of bacteriophage T7 DNA. Nucleic Acids Res (1980) 1.81
Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli. J Mol Biol (1995) 1.73
Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymers. J Control Release (2003) 1.69
Targeting bacteriophage T7 RNA polymerase to the mammalian cell nucleus. Gene (1988) 1.67
Relationships among different strains of T7 and among T7-related bacteriophages. Virology (1979) 1.61
Micro magnetic resonance angiography of the finger in systemic sclerosis. Rheumatology (Oxford) (2008) 1.58
The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase. Proc Natl Acad Sci U S A (1994) 1.58
Protein kinase induction in Escherichia coli by bacteriophage T7. Proc Natl Acad Sci U S A (1974) 1.54
Large scale purification and biochemical characterization of T7 primase/helicase proteins. Evidence for homodimer and heterodimer formation. J Biol Chem (1992) 1.52
Structural genomics of enzymes involved in sterol/isoprenoid biosynthesis. Proc Natl Acad Sci U S A (2001) 1.46
DNA sequencing using capillary array electrophoresis. Anal Chem (1992) 1.46
Purification of the gene 0.3 protein of bacteriophage T7, an inhibitor of the DNA restriction system of Escherichia coli. J Biol Chem (1981) 1.45
Biochemical analysis of mutant T7 primase/helicase proteins defective in DNA binding, nucleotide hydrolysis, and the coupling of hydrolysis with DNA unwinding. J Biol Chem (1996) 1.44
Inhibition of the type I restriction-modification enzymes EcoB and EcoK by the gene 0.3 protein of bacteriophage T7. J Mol Biol (1985) 1.42
[Correlation between magnetic resonance and disability scales (EDSS and ISS) in multiple sclerosis]. Neurologia (1991) 1.39
Pausing and termination by bacteriophage T7 RNA polymerase. J Mol Biol (1998) 1.32
HIV-1 capsid protein forms spherical (immature-like) and tubular (mature-like) particles in vitro: structure switching by pH-induced conformational changes. Biophys J (2001) 1.32
Integration of two sets of T7 mutants. Virology (1969) 1.32
Survey and mapping of restriction endonuclease cleavage sites in bacteriophage T7 DNA. J Mol Biol (1979) 1.31
Entry of bacteriophage T7 DNA into the cell and escape from host restriction. J Bacteriol (1988) 1.28
adg2-1 represents a missense mutation in the ADPG pyrophosphorylase large subunit gene of Arabidopsis thaliana. Plant J (1997) 1.27
Differentiation of intraplaque versus juxtaluminal hemorrhage/thrombus in advanced human carotid atherosclerotic lesions by in vivo magnetic resonance imaging. Circulation (2004) 1.27
Y chromosome analysis of infertile men and their sons conceived through intracytoplasmic sperm injection: vertical transmission of deletions and rarity of de novo deletions. Fertil Steril (2000) 1.25
High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange. Nucleic Acids Res (1991) 1.25
Histone H1 is located in the interior of the chromatin 30-nm filament. Nature (1994) 1.23
Mechanism of inhibition of bacteriophage T7 RNA polymerase by T7 lysozyme. J Mol Biol (1997) 1.20
Creation of a T7 autogene. Cloning and expression of the gene for bacteriophage T7 RNA polymerase under control of its cognate promoter. J Mol Biol (1991) 1.20
Downregulation and abnormal expression of E-cadherin and beta-catenin in nasopharyngeal carcinoma: close association with advanced disease stage and lymph node metastasis. Hum Pathol (1999) 1.20
Processing transcription, and translation of bacteriophage T7 messenger RNAs. Brookhaven Symp Biol (1975) 1.17
Spherical bilayer vesicles of fullerene-based surfactants in water: a laser light scattering study. Science (2001) 1.16
Picogram detection of stable dye-DNA intercalation complexes with two-color laser-excited confocal fluorescence gel scanner. Methods Enzymol (1993) 1.15
Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity. Anal Biochem (1993) 1.13
Amino acid sequence of the gene 0.3 protein of bacteriophage T7 and nucleotide sequence of its mRNA. J Biol Chem (1981) 1.12
Mutant bacteriophage T7 RNA polymerases with altered termination properties. J Mol Biol (1997) 1.11
Expression of the bisphosphatase domain of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli. Proc Natl Acad Sci U S A (1988) 1.10
Evidence for the thiamine biosynthetic pathway in higher-plant plastids and its developmental regulation. Plant Mol Biol (1995) 1.09
Comparison of the structure of myosin subfragment 1 bound to actin and free in solution. A neutron scattering study using actin made "invisible" by deuteration. J Mol Biol (1988) 1.09
Scanning transmission electron microscopy and small-angle scattering provide evidence that native Escherichia coli ClpP is a tetradecamer with an axial pore. Biochemistry (1995) 1.06
Assembly of T7 capsids from independently expressed and purified head protein and scaffolding protein. J Mol Biol (1996) 1.06
Characterization and in situ localization of a salt-induced tomato peroxidase mRNA. Plant Mol Biol (1994) 1.04
Predictors of surface disruption with MR imaging in asymptomatic carotid artery stenosis. AJNR Am J Neuroradiol (2009) 1.02
Non-steroidal anti-inflammatory drugs inhibit the expression of cytokines and induce HSP70 in human monocytes. Cytokine (1999) 1.01
Identification and mapping of five new genes in bacteriophage T7. J Mol Biol (1981) 1.01
Ligation of hexamers on hexamer templates to produce primers for cycle sequencing or the polymerase chain reaction. Anal Biochem (1995) 0.98
T7 RNA polymerase directed expression of the Escherichia coli rrnB operon. EMBO J (1986) 0.98
Human tau filaments induce microtubule and synapse loss in an in vivo model of neurofibrillary degenerative disease. J Cell Sci (2000) 0.97
Experiemental and clinical studies on muramidase (lysozyme). I. Muramidase activity of normal human blood cells and inflammatory exudates. Acta Haematol (1970) 0.97
Self-association of beta-lactoglobulin A in acid solution. I. Translational diffusion coefficients. Biopolymers (1975) 0.96
Cloning and expression of gene 4 of bacteriophage T7 and creation and analysis of T7 mutants lacking the 4A primase/helicase or the 4B helicase. J Biol Chem (1992) 0.96
The effect of regulatory Ca2+ on the in situ structures of troponin C and troponin I: a neutron scattering study. J Mol Biol (1998) 0.95
Conformations of myosin subfragment 1 ATPase intermediates from neutron and X-ray scattering. J Mol Biol (1996) 0.95
Shoot regeneration and Agrobacterium-mediated transformation of Fragaria vesca L. Plant Cell Rep (1996) 0.94
Cloning of the T7 genome in Escherichia coli: use of recombination between cloned sequences and bacteriophage T7 to identify genes involved in recombination and a clone containing the origin of T7 DNA replication. Cold Spring Harb Symp Quant Biol (1979) 0.94
Resolution of branched DNA substrates by T7 endonuclease I and its inhibition. J Biol Chem (1991) 0.93