A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells.

PubWeight™: 1.26‹?› | Rank: Top 10%

🔗 View Article (PMC 147110)

Published in Nucleic Acids Res on December 01, 1997

Authors

G M Lamm1, P Steinlein, M Cotten, G Christofori

Author Affiliations

1: Research Institute of Molecular Pathology (I. M. P.), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.

Articles citing this

p73 function is inhibited by tumor-derived p53 mutants in mammalian cells. Mol Cell Biol (1999) 2.41

Curcumin treatment abrogates endoplasmic reticulum retention and aggregation-induced apoptosis associated with neuropathy-causing myelin protein zero-truncating mutants. Am J Hum Genet (2005) 1.11

Alternatively spliced products CC3 and TC3 have opposing effects on apoptosis. Mol Cell Biol (2000) 1.05

Interaction between tumor suppressor adenomatous polyposis coli and topoisomerase IIalpha: implication for the G2/M transition. Mol Biol Cell (2008) 0.99

Urokinase receptor-dependent and -independent p56/59(hck) activation state is a molecular switch between myelomonocytic cell motility and adherence. EMBO J (1999) 0.96

An improved non-enzymatic "DNA ladder assay" for more sensitive and early detection of apoptosis. Cytotechnology (2011) 0.86

The BH3 domain of Bcl-x(S) is required for inhibition of the antiapoptotic function of Bcl-x(L). Mol Cell Biol (1999) 0.86

Assessment of GFP expression and viability using the tali image-based cytometer. J Vis Exp (2011) 0.83

PARP targeting counteracts gliomagenesis through induction of mitotic catastrophe and aggravation of deficiency in homologous recombination in PTEN-mutant glioma. Oncotarget (2015) 0.82

Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level. PLoS One (2014) 0.80

Effects of orientin and vitexin from Trollius chinensis on the growth and apoptosis of esophageal cancer EC-109 cells. Oncol Lett (2015) 0.80

Conserved function of caspase-8 in apoptosis during bony fish evolution. Gene (2007) 0.79

Construction of avian adenovirus CELO recombinants in cosmids. J Virol (2001) 0.79

The evolutionary conservation of the core components necessary for the extrinsic apoptotic signaling pathway, in Medaka fish. BMC Genomics (2007) 0.79

Plasma membrane phosphatidylinositol 4,5 bisphosphate is required for internalization of foot-and-mouth disease virus and vesicular stomatitis virus. PLoS One (2012) 0.77

Concentric Magnetic Structures for Magnetophoretic Bead Collection, Cell Trapping and Analysis of Cell Morphological Changes Caused by Local Magnetic Forces. PLoS One (2015) 0.75

Articles cited by this

Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol (1992) 34.17

FACS-optimized mutants of the green fluorescent protein (GFP). Gene (1996) 24.12

Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature (1980) 17.69

A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods (1991) 16.64

Heritable formation of pancreatic beta-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes. Nature (1985) 9.78

Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood (1994) 9.10

Features of apoptotic cells measured by flow cytometry. Cytometry (1992) 6.45

Life, death, and the pursuit of apoptosis. Genes Dev (1996) 6.21

Programmed cell death and the control of cell survival: lessons from the nervous system. Science (1993) 4.63

The molecular biology of apoptosis. Proc Natl Acad Sci U S A (1996) 3.39

Adenovirus E1B 19-kilodalton protein overcomes the cytotoxicity of E1A proteins. J Virol (1991) 2.71

Regulators of cell death. Trends Genet (1995) 2.37

Role of adenovirus E1B proteins in transformation: altered organization of intermediate filaments in transformed cells that express the 19-kilodalton protein. Mol Cell Biol (1990) 2.37

The end of the (cell) line: methods for the study of apoptosis in vitro. Methods Cell Biol (1995) 2.32

Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry (1992) 2.30

The cell-death machine. Curr Biol (1996) 2.18

Role of Ced-3/ICE-family proteases in staurosporine-induced programmed cell death. J Cell Biol (1996) 2.17

Anatomical methods in cell death. Methods Cell Biol (1995) 1.64

Evaluation of glucocorticoid-induced DNA fragmentation in mouse thymocytes by flow cytometry. Cell Prolif (1991) 1.60

Pancreatic beta cells cultured from individual preneoplastic foci in a multistage tumorigenesis pathway: a potentially general technique for isolating physiologically representative cell lines. Mol Cell Biol (1993) 1.34

Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. Gene Ther (1997) 1.29

Quantitation of DNA fragmentation in apoptosis. Nucleic Acids Res (1996) 1.22

An ELISA for detection of apoptosis. Nucleic Acids Res (1997) 1.03

A new approach for the electrophoretic detection of apoptosis. Nucleic Acids Res (1996) 0.88

Articles by these authors

A causal role for E-cadherin in the transition from adenoma to carcinoma. Nature (1998) 8.48

Vascular endothelial growth factor-C-mediated lymphangiogenesis promotes tumour metastasis. EMBO J (2001) 4.70

Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA. EMBO J (1991) 2.94

Transferrin-polycation conjugates as carriers for DNA uptake into cells. Proc Natl Acad Sci U S A (1990) 2.64

N-CAM modulates tumour-cell adhesion to matrix by inducing FGF-receptor signalling. Nat Cell Biol (2001) 2.57

The potential role of podoplanin in tumour invasion. Br J Cancer (2006) 2.20

The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther (1998) 2.19

Betacellulin: a mitogen from pancreatic beta cell tumors. Science (1993) 2.10

Antiangiogenic therapy of transgenic mice impairs de novo tumor growth. Proc Natl Acad Sci U S A (1996) 2.07

Metastasis: cell-autonomous mechanisms versus contributions by the tumor microenvironment. Cell Mol Life Sci (2006) 2.04

Mammalian sprouty-1 and -2 are membrane-anchored phosphoprotein inhibitors of growth factor signaling in endothelial cells. J Cell Biol (2001) 2.02

Transgenic mouse models of tumour angiogenesis: the angiogenic switch, its molecular controls, and prospects for preclinical therapeutic models. Eur J Cancer (1996) 1.92

The glucocorticoid receptor is required for stress erythropoiesis. Genes Dev (1999) 1.92

Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus. Gene Ther (2000) 1.90

Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle. Proc Natl Acad Sci U S A (1992) 1.87

The glucocorticoid receptor cooperates with the erythropoietin receptor and c-Kit to enhance and sustain proliferation of erythroid progenitors in vitro. Blood (1999) 1.87

Adenovirus enhancement of transferrin-polylysine-mediated gene delivery. Proc Natl Acad Sci U S A (1991) 1.85

Conditional and specific NF-kappaB blockade protects pancreatic beta cells from diabetogenic agents. Proc Natl Acad Sci U S A (2006) 1.85

Transferrin-polycation-DNA complexes: the effect of polycations on the structure of the complex and DNA delivery to cells. Proc Natl Acad Sci U S A (1991) 1.80

Cleavage and polyadenylation of messenger RNA precursors in vitro occurs within large and specific 3' processing complexes. EMBO J (1987) 1.78

Fos is an essential component of the mammalian UV response. EMBO J (1995) 1.73

Cell adhesion in tumor invasion and metastasis: loss of the glue is not enough. Biochim Biophys Acta (2001) 1.72

Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. Proc Natl Acad Sci U S A (1992) 1.72

DNA/polyethylenimine transfection particles: influence of ligands, polymer size, and PEGylation on internalization and gene expression. AAPS PharmSci (2001) 1.66

Fibroblast growth factors are required for efficient tumor angiogenesis. Cancer Res (2000) 1.65

Receptor-mediated endocytosis of transferrin-polycation conjugates: an efficient way to introduce DNA into hematopoietic cells. Proc Natl Acad Sci U S A (1990) 1.64

Lipopolysaccharide induces in macrophages the synthesis of the suppressor of cytokine signaling 3 and suppresses signal transduction in response to the activating factor IFN-gamma. J Immunol (1999) 1.61

Noncontact dipole effects on channel permeation. I. Experiments with (5F-indole)Trp13 gramicidin A channels. Biophys J (1998) 1.57

Noncontact dipole effects on channel permeation. III. Anomalous proton conductance effects in gramicidin. Biophys J (2008) 1.54

Independent regulation of the two Pax5 alleles during B-cell development. Nat Genet (1999) 1.54

Tumor suppressor loci on mouse chromosomes 9 and 16 are lost at distinct stages of tumorigenesis in a transgenic model of islet cell carcinoma. Cancer Res (1995) 1.47

High-efficiency gene transfer mediated by adenovirus coupled to DNA-polylysine complexes. Hum Gene Ther (1992) 1.45

Correction of hyperglycemia in diabetic mice transplanted with reversibly immortalized pancreatic beta cells controlled by the tet-on regulatory system. Cell Transplant (2001) 1.39

Reduced expression of neural cell adhesion molecule induces metastatic dissemination of pancreatic beta tumor cells. Nat Med (1999) 1.39

Characterization of early and late endocytic compartments of the transferrin cycle. Transferrin receptor antibody blocks erythroid differentiation by trapping the receptor in the early endosome. J Cell Sci (1992) 1.37

Gene transfer into hepatocytes using asialoglycoprotein receptor mediated endocytosis of DNA complexed with an artificial tetra-antennary galactose ligand. Bioconjug Chem (1993) 1.35

Activation of heat-shock response by an adenovirus is essential for virus replication. Nature (2000) 1.34

Neural cell adhesion molecule (N-CAM) is required for cell type segregation and normal ultrastructure in pancreatic islets. J Cell Biol (1999) 1.34

Conformational trapping in a membrane environment: a regulatory mechanism for protein activity? Proc Natl Acad Sci U S A (1996) 1.30

The complete DNA sequence and genomic organization of the avian adenovirus CELO. J Virol (1996) 1.30

Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. Gene Ther (1997) 1.29

Recent advances in research on multistage tumorigenesis. Br J Cancer (2000) 1.26

The tumor-suppressor function of E-cadherin. Am J Hum Genet (1998) 1.25

Listeria monocytogenes modulates macrophage cytokine responses through STAT serine phosphorylation and the induction of suppressor of cytokine signaling 3. J Immunol (2001) 1.22

Tumor progression induced by the loss of E-cadherin independent of beta-catenin/Tcf-mediated Wnt signaling. Oncogene (2006) 1.22

DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety. Bioconjug Chem (1992) 1.21

T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol (1999) 1.19

Xenopus nucleoplasmin: egg vs. oocyte. Biochemistry (1986) 1.18

Novobiocin precipitates histones at concentrations normally used to inhibit eukaryotic type II topoisomerase. Nucleic Acids Res (1986) 1.17

Mutational analysis of the avian adenovirus CELO, which provides a basis for gene delivery vectors. J Virol (1999) 1.15

Molecular mechanisms of tumor angiogenesis and tumor progression. J Neurooncol (2001) 1.12

Mannose polyethylenimine conjugates for targeted DNA delivery into dendritic cells. J Biol Chem (1999) 1.11

Identification of syntenin as a protein of the apical early endocytic compartment in Madin-Darby canine kidney cells. J Biol Chem (1999) 1.08

Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs. Biochemistry (1986) 1.06

Microtubule-independent motility and nuclear targeting of adenoviruses with fluorescently labeled genomes. J Virol (2001) 1.03

Noncontact dipole effects on channel permeation. IV. Kinetic model of 5F-Trp(13) gramicidin A currents. Biophys J (2001) 1.02

Self-renewal and differentiation of normal avian erythroid progenitor cells: regulatory roles of the TGF alpha/c-ErbB and SCF/c-kit receptors. Cell (1993) 1.01

Molecular cloning of mouse pancreatic islet R-cadherin: differential expression in endocrine and exocrine tissue. Mol Endocrinol (1993) 1.01

Elicitation of a systemic and protective anti-melanoma immune response by an IL-2-based vaccine. Assessment of critical cellular and molecular parameters. J Immunol (1995) 1.00

MHC class II presentation of endogenously expressed antigens by transfected dendritic cells. Gene Ther (2001) 0.99

Delivery of bacterial artificial chromosomes into mammalian cells with psoralen-inactivated adenovirus carrier. Nucleic Acids Res (1997) 0.98

Novobiocin inhibits passive chromatin assembly in vitro. EMBO J (1986) 0.97

Receptor-mediated gene transfer into human T lymphocytes via binding of DNA/CD3 antibody particles to the CD3 T cell receptor complex. Hum Gene Ther (1995) 0.97

Identification of a novel antiapoptotic protein, GAM-1, encoded by the CELO adenovirus. J Virol (1997) 0.96

Direct in vivo gene transfer to airway epithelium employing adenovirus-polylysine-DNA complexes. Hum Gene Ther (1993) 0.96

Two spliceosomes can form simultaneously and independently on synthetic double-intron messenger RNA precursors. EMBO J (1987) 0.94

Efficient gene delivery into human dendritic cells by adenovirus polyethylenimine and mannose polyethylenimine transfection. Hum Gene Ther (1999) 0.94

Rescuing the neonatal brain from hypoxic injury with autologous cord blood. Bone Marrow Transplant (2012) 0.93

A novel way to induce erythroid progenitor self renewal: cooperation of c-Kit with the erythropoietin receptor. Biol Chem (1999) 0.92

Hyperacetylated histones facilitate chromatin assembly in vitro. Nucleic Acids Res (1985) 0.91

In vitro growth of factor-dependent multipotential hematopoietic cells is induced by the nuclear oncoprotein v-Ski. Oncogene (1995) 0.90

Purification of Xenopus egg nucleoplasmin and its use in chromatin assembly in vitro. Methods Enzymol (1989) 0.90

Defining CAR as a cellular receptor for the avian adenovirus CELO using a genetic analysis of the two viral fibre proteins. J Gen Virol (2001) 0.90

Cell biologists sort things out: Analysis and purification of intracellular organelles by flow cytometry. Trends Cell Biol (1997) 0.89

A versatile assay to study cellular uptake of gene transfer complexes by flow cytometry. Biochim Biophys Acta (2000) 0.89

Transferrinfection: a highly efficient way to express gene constructs in eukaryotic cells. Ann N Y Acad Sci (1992) 0.89

Neisseria gonorrhoeae porin modulates phagosome maturation. J Biol Chem (1998) 0.88

Avian hematopoietic cell culture: in vitro model systems to study oncogenic transformation of hematopoietic cells. Methods Enzymol (1995) 0.88

Transformation of chicken bone marrow cells by the v-ski oncogene. Oncogene (1993) 0.87

Somatic gene therapy for cancer: the utility of transferrinfection in generating 'tumor vaccines'. Gene (1993) 0.87

Keratinocyte-specific onset of serine protease BSSP expression in experimental carcinogenesis. J Invest Dermatol (2001) 0.86

Avian erythropoiesis and erythroleukemia: towards understanding the role of the biomolecules involved. Biochim Biophys Acta (1996) 0.85

Impairment of survival factor function potentiates chemotherapy-induced apoptosis in tumor cells. Cancer Res (1998) 0.85

Hybrid proteins between Pseudomonas aeruginosa exotoxin A and poliovirus 2Apro cleave p220 in HeLa cells. J Virol (1996) 0.85

Application of a novel, rapid, and sensitive oligonucleotide ligation assay for detection of cancer-predicting mutations in the precore and basal core promoter of hepatitis B virus. J Clin Microbiol (2008) 0.85

In vivo production of human factor VII in mice after intrasplenic implantation of primary fibroblasts transfected by receptor-mediated, adenovirus-augmented gene delivery. Proc Natl Acad Sci U S A (1994) 0.84

Characterization of CELO virus proteins that modulate the pRb/E2F pathway. J Virol (1999) 0.84

Synthesis and expression of MHC class II molecules in the absence of attached invariant chains by recombinant-interferon-gamma-activated bone-marrow-derived macrophages. Eur J Immunol (1987) 0.83

An unexpected role for p53 in augmenting SV40 large T antigen-mediated tumorigenesis. Biol Chem (1999) 0.83

Amphibian oocytes and sphere organelles: are the U snRNA genes amplified? Chromosoma (1992) 0.82

Insulin-like growth factor II is focally up-regulated and functionally involved as a second signal for oncogene-induced tumorigenesis. Cold Spring Harb Symp Quant Biol (1994) 0.81

Formation of transformed endothelial cells in the absence of VEGFR-2/Flk-1 by Polyoma middle T oncogene. Oncogene (1999) 0.81

High-level expression of various apolipoprotein(a) isoforms by "transferrinfection": the role of kringle IV sequences in the extracellular association with low-density lipoprotein. Biochemistry (1994) 0.80

Hepatocyte growth factor protects hepatoblastoma cells from chemotherapy-induced apoptosis by AKT activation. Int J Oncol (2010) 0.80

Inter- and intramolecular distance measurements by solid-state MAS NMR: determination of gramicidin A channel dimer structure in hydrated phospholipid bilayers. J Biomol NMR (2000) 0.79