Overexpression of human procarboxypeptidase A2 in Pichia pastoris and detailed characterization of its activation pathway.

PubWeight™: 0.83‹?›

🔗 View Article (PMID 9452479)

Published in J Biol Chem on February 06, 1998

Authors

D Reverter1, S Ventura, V Villegas, J Vendrell, F X Avilés

Author Affiliations

1: Departament de Bioquímica i Biologia Molecular, Unitat de Ciències and the Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.

Articles by these authors

Circulating interleukin 6 levels, blood pressure, and insulin sensitivity in apparently healthy men and women. J Clin Endocrinol Metab (2001) 2.22

Identification of novel genes that co-cluster with estrogen receptor alpha in breast tumor biopsy specimens, using a large-scale real-time reverse transcription-PCR approach. Endocr Relat Cancer (2006) 1.96

Prevalence of diabetes mellitus and impaired glucose regulation in Spain: the Di@bet.es Study. Diabetologia (2011) 1.94

An automated classification of the structure of protein loops. J Mol Biol (1997) 1.77

Interleukin-6 gene polymorphism and lipid abnormalities in healthy subjects. J Clin Endocrinol Metab (2000) 1.61

Interleukin-6 gene polymorphism and insulin sensitivity. Diabetes (2000) 1.58

On the sensitivity of MD trajectories to changes in water-protein interaction parameters: the potato carboxypeptidase inhibitor in water as a test case for the GROMOS force field. Proteins (1996) 1.57

Polymorphism of the tumor necrosis factor-alpha receptor 2 gene is associated with obesity, leptin levels, and insulin resistance in young subjects and diet-treated type 2 diabetic patients. Diabetes Care (2000) 1.56

Structure of the transition state in the folding process of human procarboxypeptidase A2 activation domain. J Mol Biol (1998) 1.47

Protein engineering as a strategy to avoid formation of amyloid fibrils. Protein Sci (2000) 1.46

Observation of a centrality-dependent dijet asymmetry in lead-lead collisions at sqrt[S(NN)] =2.76 TeV with the ATLAS detector at the LHC. Phys Rev Lett (2010) 1.41

Metallocarboxypeptidases and their protein inhibitors. Structure, function and biomedical properties. Biochim Biophys Acta (2000) 1.36

Stromal stem cells from adipose tissue and bone marrow of age-matched female donors display distinct immunophenotypic profiles. J Cell Physiol (2011) 1.36

Late-onset hypocalcemia appearing years after thyroid surgery. J Endocrinol Invest (1989) 1.34

Search for new particles in two-jet final states in 7 TeV proton-proton collisions with the ATLAS detector at the LHC. Phys Rev Lett (2010) 1.32

Three-dimensional structure of porcine procarboxypeptidase B: a structural basis of its inactivity. EMBO J (1991) 1.30

Circulating and adipose tissue gene expression of zinc-alpha2-glycoprotein in obesity: its relationship with adipokine and lipolytic gene markers in subcutaneous and visceral fat. J Clin Endocrinol Metab (2009) 1.25

Three-dimensional structure of porcine pancreatic procarboxypeptidase A. A comparison of the A and B zymogens and their determinants for inhibition and activation. J Mol Biol (1992) 1.25

Plasma levels of the soluble fraction of tumor necrosis factor receptor 2 and insulin resistance. Diabetes (1998) 1.23

The three-dimensional structure of human procarboxypeptidase A2. Deciphering the basis of the inhibition, activation and intrinsic activity of the zymogen. EMBO J (1997) 1.21

Obesity and insulin resistance-related changes in the expression of lipogenic and lipolytic genes in morbidly obese subjects. Obes Surg (2010) 1.14

Metabolic endotoxemia and saturated fat contribute to circulating NGAL concentrations in subjects with insulin resistance. Int J Obes (Lond) (2009) 1.14

Inhibition of carboxypeptidase A by excess zinc: analysis of the structural determinants by X-ray crystallography. FEBS Lett (1997) 1.11

Automated classification of antibody complementarity determining region 3 of the heavy chain (H3) loops into canonical forms and its application to protein structure prediction. J Mol Biol (1998) 1.08

The tryptic activation pathway of monomeric procarboxypeptidase A. J Biol Chem (1990) 1.07

Advances in metallo-procarboxypeptidases. Emerging details on the inhibition mechanism and on the activation process. Eur J Biochem (1993) 1.07

The TNF-alpha gene Nco I polymorphism influences the relationship among insulin resistance, percent body fat, and increased serum leptin levels. Diabetes (1997) 1.06

Favourable native-like helical local interactions can accelerate protein folding. Fold Des (1997) 1.06

The three-dimensional structure of the native ternary complex of bovine pancreatic procarboxypeptidase A with proproteinase E and chymotrypsinogen C. EMBO J (1995) 1.06

The autonomic and sensory innervation of the smooth muscle of the prostate gland: a review of pharmacological and histological studies. J Auton Pharmacol (2000) 1.06

Crystal structure of avian carboxypeptidase D domain II: a prototype for the regulatory metallocarboxypeptidase subfamily. EMBO J (1999) 1.05

The severed activation segment of porcine pancreatic procarboxypeptidase A is a powerful inhibitor of the active enzyme. Isolation and characterisation of the activation peptide. Biochim Biophys Acta (1982) 1.04

Stabilization of proteins by rational design of alpha-helix stability using helix/coil transition theory. Fold Des (1996) 1.03

Diabetic neuropathy is associated with activation of the TNF-alpha system in subjects with type 1 diabetes mellitus. Clin Endocrinol (Oxf) (2005) 1.03

Lower heart rate variability is associated with higher plasma concentrations of IL-6 in type 1 diabetes. Eur J Endocrinol (2007) 1.02

Analysis of the activation process of porcine procarboxypeptidase B and determination of the sequence of its activation segment. Biochemistry (1991) 1.02

Evidence for a two-state transition in the folding process of the activation domain of human procarboxypeptidase A2. Biochemistry (1995) 1.01

Primary structure of the activation segment of procarboxypeptidase A from porcine pancreas. Biochem Biophys Res Commun (1986) 1.00

The alpha1A-adrenoceptor gene is required for the alpha1L-adrenoceptor-mediated response in isolated preparations of the mouse prostate. Br J Pharmacol (2008) 1.00

Occupational health and safety status of indigenous and Latino farmworkers in Oregon. J Agric Saf Health (2009) 1.00

The crystal structure of the inhibitor-complexed carboxypeptidase D domain II and the modeling of regulatory carboxypeptidases. J Biol Chem (2001) 0.98

Structure of a novel leech carboxypeptidase inhibitor determined free in solution and in complex with human carboxypeptidase A2. Nat Struct Biol (2000) 0.96

Expression of TWEAK and its receptor Fn14 in human subcutaneous adipose tissue. Relationship with other inflammatory cytokines in obesity. Cytokine (2006) 0.96

Plasma lipid lowering activity of acipimox in patients with type II and type IV hyperlipoproteinemia. Results of a multicenter trial. Atherosclerosis (1988) 0.96

Plasma PTX3 protein levels inversely correlate with insulin secretion and obesity, whereas visceral adipose tissue PTX3 gene expression is increased in obesity. Am J Physiol Endocrinol Metab (2011) 0.95

Neural mechanisms in the cardiovascular responses to acute central hypovolaemia. Clin Exp Pharmacol Physiol (2001) 0.94

The disulfide folding pathway of potato carboxypeptidase inhibitor. J Biol Chem (1994) 0.92

Effect of weight loss induced by gastric bypass on proinflammatory interleukin-18, soluble tumour necrosis factor-alpha receptors, C-reactive protein and adiponectin in morbidly obese patients. Clin Endocrinol (Oxf) (2007) 0.92

Adipose tissue expression of the glycerol channel aquaporin-7 gene is altered in severe obesity but not in type 2 diabetes. J Clin Endocrinol Metab (2007) 0.92

A carboxypeptidase inhibitor from the medical leech Hirudo medicinalis. Isolation, sequence analysis, cDNA cloning, recombinant expression, and characterization. J Biol Chem (1998) 0.91

Pharmacology of neurotransmission to the smooth muscle of the rat and the guinea-pig prostate glands. J Auton Pharmacol (1998) 0.91

Circulating IL-18 concentration is associated with insulin sensitivity and glucose tolerance through increased fat-free mass. Diabetologia (2005) 0.90

Classification of protein disulphide-bridge topologies. J Comput Aided Mol Des (2001) 0.90

Tumour necrosis factor alpha in fat redistribution syndromes associated with combination antiretroviral therapy in HIV-1-infected patients: potential role in subcutaneous adipocyte apoptosis. Eur J Clin Invest (2005) 0.90

Zinc alpha-2 glycoprotein is implicated in dyslipidaemia in HIV-1-infected patients treated with antiretroviral drugs. HIV Med (2012) 0.89

Paired subcutaneous and visceral adipose tissue aquaporin-7 expression in human obesity and type 2 diabetes: differences and similarities between depots. J Clin Endocrinol Metab (2010) 0.89

Smoking, fat mass and activation of the tumor necrosis factor-alpha pathway. Int J Obes Relat Metab Disord (2003) 0.89

Potato carboxypeptidase inhibitor, a T-knot protein, is an epidermal growth factor antagonist that inhibits tumor cell growth. J Biol Chem (1998) 0.88

Relation between human LPIN1, hypoxia and endoplasmic reticulum stress genes in subcutaneous and visceral adipose tissue. Int J Obes (Lond) (2010) 0.88

Protection from inflammatory disease in insulin resistance: the role of mannan-binding lectin. Diabetologia (2006) 0.88

Lower rate of tumor necrosis factor-alpha -863A allele and higher concentration of tumor necrosis factor-alpha receptor 2 in first-degree relatives of subjects with type 2 diabetes. Metabolism (2003) 0.88

Maternal soluble tumour necrosis factor receptor type 2 (sTNFR2) and adiponectin are both related to blood pressure during gestation and infant's birthweight. Clin Endocrinol (Oxf) (2004) 0.87

'TransMem': a neural network implemented in Excel spreadsheets for predicting transmembrane domains of proteins. Comput Appl Biosci (1997) 0.87

Early kinetics of amyloid fibril formation reveals conformational reorganisation of initial aggregates. J Mol Biol (2006) 0.87

Human serum levels of fetal antigen 1 (FA1/Dlk1) increase with obesity, are negatively associated with insulin sensitivity and modulate inflammation in vitro. Int J Obes (Lond) (2008) 0.87

Protein similarities beyond disulphide bridge topology. J Mol Biol (1998) 0.87

The usefulness of HbA1c in postpartum reclassification of gestational diabetes. BJOG (2012) 0.87

Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzyme. J Biol Chem (1999) 0.87

The sequence and conformation of human pancreatic procarboxypeptidase A2. cDNA cloning, sequence analysis, and three-dimensional model. J Biol Chem (1995) 0.87

Crystal structure of an oligomer of proteolytic zymogens: detailed conformational analysis of the bovine ternary complex and implications for their activation. J Mol Biol (1997) 0.87

Plasma visfatin concentrations increase in both hyper and hypothyroid subjects after normalization of thyroid function and are not related to insulin resistance, anthropometric or inflammatory parameters. Clin Endocrinol (Oxf) (2009) 0.86

Pattern and pathways for mercury lifespan bioaccumulation in Carcinus maenas. Mar Pollut Bull (2008) 0.86

Thermodynamic analysis of helix-engineered forms of the activation domain of human procarboxypeptidase A2. Eur J Biochem (2000) 0.86

Comparison between the monomeric and binary-complex forms of procarboxypeptidase A from whole pig pancreas. Biochem J (1981) 0.86

Oligonucleotide microarray analysis of estrogen receptor alpha-positive postmenopausal breast carcinomas: identification of HRPAP20 and TIMELESS as outstanding candidate markers to predict the response to tamoxifen. J Mol Endocrinol (2007) 0.86

Comparison of the NMR solution structure with the X-ray crystal structure of the activation domain from procarboxypeptidase B. J Biomol NMR (1992) 0.86

Isolation and re-association of the subunits from the pro-(carboxypeptidase A)-pro-(proteinase E) binary complex from pgi pancreas. Biochem J (1982) 0.86

Preparative isolation of the two forms of pig pancreatic pro-(carboxypeptidase A) and their monomeric carboxypeptidases A. Biochem J (1985) 0.85

The NMR structure of the activation domain isolated from porcine procarboxypeptidase B. EMBO J (1991) 0.85

Bone mineral mass is associated with interleukin 1 receptor autoantigen and TNF-alpha gene polymorphisms in post-menopausal Mediterranean women. J Endocrinol Invest (2002) 0.85

The tumour necrosis factor (TNF)-alpha system is activated in accordance with pulse pressure in normotensive subjects with type 1 diabetes mellitus. Eur J Endocrinol (2005) 0.85

Urea-gradient gel electrophoresis studies on the association of procarboxypeptidases A and B, proproteinase E, and their tryptic activation products. FEBS Lett (1985) 0.85

Diabetes in AIDS patients. Lancet (1988) 0.84

Effect of the reaction field electrostatic term on the molecular dynamics simulation of the activation domain of procarboxypeptidase B. Protein Eng (2000) 0.84

Genetic variation in promoter (4G/5G) of plasminogen activator inhibitor 1 gene in type 2 diabetes. Absence of relationship with microangiopathy. Diabetes Care (1998) 0.84