Published in Proc Natl Acad Sci U S A on August 18, 1998
Lessons from lactose permease. Annu Rev Biophys Biomol Struct (2006) 3.69
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY. EMBO J (2006) 2.92
Lactose permease and the alternating access mechanism. Biochemistry (2011) 1.62
The alternating access transport mechanism in LacY. J Membr Biol (2010) 1.41
Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (2003) 1.36
Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition. Proc Natl Acad Sci U S A (2011) 1.33
Direct sugar binding to LacY measured by resonance energy transfer. Biochemistry (2006) 1.32
Arg-302 facilitates deprotonation of Glu-325 in the transport mechanism of the lactose permease from Escherichiacoli. Proc Natl Acad Sci U S A (2001) 1.28
Protonation and sugar binding to LacY. Proc Natl Acad Sci U S A (2008) 1.25
Unraveling the mechanism of the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (2000) 1.24
Structure of sugar-bound LacY. Proc Natl Acad Sci U S A (2014) 1.23
Sugar binding and protein conformational changes in lactose permease. Biophys J (2006) 1.20
Residues in the H+ translocation site define the pKa for sugar binding to LacY. Biochemistry (2009) 1.17
Elucidation of substrate binding interactions in a membrane transport protein by mass spectrometry. EMBO J (2003) 1.16
Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane. Proc Natl Acad Sci U S A (2010) 1.11
Sequence alignment and homology threading reveals prokaryotic and eukaryotic proteins similar to lactose permease. J Mol Biol (2006) 1.09
Changing the lactose permease of Escherichia coli into a galactose-specific symporter. Proc Natl Acad Sci U S A (2002) 1.04
Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease. J Mol Biol (2006) 1.04
Conformational flexibility at the substrate binding site in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (1999) 1.00
Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site. Proc Natl Acad Sci U S A (2015) 0.88
Substrate-induced Unlocking of the Inner Gate Determines the Catalytic Efficiency of a Neurotransmitter:Sodium Symporter. J Biol Chem (2015) 0.86
Deduction of consensus binding sequences on proteins that bind IIAGlc of the phosphoenolpyruvate:sugar phosphotransferase system by cysteine scanning mutagenesis of Escherichia coli lactose permease. Proc Natl Acad Sci U S A (1999) 0.85
Inducible L-alanine exporter encoded by the novel gene ygaW (alaE) in Escherichia coli. Appl Environ Microbiol (2011) 0.84
The citrate carrier CitS probed by single-molecule fluorescence spectroscopy. Biophys J (2003) 0.77
A suppressor analysis of residues involved in cation transport in the lactose permease: identification of a coupling sensor. J Membr Biol (2006) 0.77
Galactoside-binding site in LacY. Biochemistry (2014) 0.77
DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A (1977) 790.54
Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene (1989) 60.30
Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli. Proc Natl Acad Sci U S A (1965) 7.81
Structure of the lac carrier protein of Escherichia coli. J Biol Chem (1983) 2.92
The lac carrier protein in Escherichia coli. J Membr Biol (1983) 2.29
Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli. Protein Sci (1993) 1.78
Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli. Proc Natl Acad Sci U S A (1994) 1.68
Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes. Proc Natl Acad Sci U S A (1984) 1.68
Cysteine 148 in the lactose permease of Escherichia coli is a component of a substrate binding site. 2. Site-directed fluorescence studies. Biochemistry (1994) 1.44
Properties and purification of an active biotinylated lactose permease from Escherichia coli. Proc Natl Acad Sci U S A (1993) 1.43
Probing the conformation of the lactose permease of Escherichia coli by in situ site-directed sulfhydryl modification. Biochemistry (1996) 1.39
A molecular mechanism for energy coupling in a membrane transport protein, the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (1997) 1.35
Cysteine-scanning mutagenesis of helix IV and the adjoining loops in the lactose permease of Escherichia coli: Glu126 and Arg144 are essential. off. Biochemistry (1997) 1.33
Exchange, efflux, and substrate binding by cysteine mutants of the lactose permease of Escherichia coli. Biochemistry (1993) 1.30
beta-D-Galactoside transport in Escherichia coli: substrate recognition. Eur J Biochem (1977) 1.30
Cysteine-scanning mutagenesis of putative helix VII in the lactose permease of Escherichia coli. Biochemistry (1994) 1.24
Role of conserved arginine and glutamate residues on the cytosolic surface of glucose transporters for transporter function. Biochemistry (1997) 1.23
Cysteine 148 in the lactose permease of Escherichia coli is a component of a substrate binding site. 1. Site-directed mutagenesis studies. Biochemistry (1994) 1.20
Ligand-induced conformational changes in the lactose permease of Escherichia coli: evidence for two binding sites. Protein Sci (1994) 1.18
Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli. Curr Opin Struct Biol (1997) 1.17
Kinetic analysis of lactose exchange in proteoliposomes reconstituted with purified lac permease. Biochemistry (1991) 1.14
Analysis of the structural specificity of the lactose permease toward sugars. J Biol Chem (1989) 1.09
Use of designed metal-binding sites to study helix proximity in the lactose permease of Escherichia coli. 1. Proximity of helix VII (Asp237 and Asp240) with helices X (Lys319) and XI (Lys358). Biochemistry (1995) 1.08
Fluorescence of native single-Trp mutants in the lactose permease from Escherichia coli: structural properties and evidence for a substrate-induced conformational change. Protein Sci (1995) 1.08
Effects of temperature on the fluorescence intensity and anisotropy decays of staphylococcal nuclease and the less stable nuclease-conA-SG28 mutant. Biochemistry (1991) 0.98
Binding of p-nitrophenyl alpha-D-galactopyranoside to lac permease of Escherichia coli. Biochemistry (1990) 0.86
Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and beta-pancreatic islet cells. Cell (1988) 5.18
The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coli. J Biol Chem (1968) 4.60
Transport across isolated bacterial cytoplasmic membranes. Biochim Biophys Acta (1972) 4.43
Proline uptake by an isolated cytoplasmic membrane preparation of Escherichia coli. Proc Natl Acad Sci U S A (1966) 4.37
Transport. Annu Rev Biochem (1970) 4.09
The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A (1976) 3.91
Mechanisms of active transport in isolated bacterial membrane vesicles. 8. The transport of amino acids by membranes prepared from Escherichia coli. J Biol Chem (1972) 3.65
Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins. FASEB J (1998) 3.41
Membrane potential and active transport in membrane vesicles from Escherichia coli. Biochemistry (1975) 3.36
Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli. J Biol Chem (1971) 3.24
Mechanisms of active transport in isolated membrane vesicles. I. The site of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in Escherichia coli membrane vesicles. J Biol Chem (1971) 2.94
Purification and reconstitution of functional lactose carrier from Escherichia coli. J Biol Chem (1981) 2.93
Structure of the lac carrier protein of Escherichia coli. J Biol Chem (1983) 2.92
Mechanisms of active transport in isolated membrane vesicles. 2. The coupling of reduced phenazine methosulfate to the concentrative uptake of beta-galactosides and amino acids. J Biol Chem (1971) 2.78
Transport in isolated bacterial membrane vesicles. Methods Enzymol (1974) 2.75
Membrane potential and gentamicin uptake in Staphylococcus aureus. Proc Natl Acad Sci U S A (1982) 2.73
The relationship between the electrochemical proton gradient and active transport in Escherichia coli membrane vesicles. Biochemistry (1977) 2.70
Quantitative measurements of membrane potential in Escherichia coli. Biochemistry (1980) 2.66
Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles. J Biol Chem (1975) 2.64
Relationship of a membrane-bound D-(-)-lactic dehydrogenase to amino acid transport in isolated bacterial membrane preparations. Proc Natl Acad Sci U S A (1970) 2.63
Mechanisms of active transport in isolated bacterial membrane vesicles. 18. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone. Arch Biochem Biophys (1974) 2.61
Transport studies in bacterial membrane vesicles. Science (1974) 2.54
Purification, reconstitution, and characterization of the lac permease of Escherichia coli. Methods Enzymol (1986) 2.32
The lac carrier protein in Escherichia coli. J Membr Biol (1983) 2.29
Proline uptake by disrupted membrane preparations from Escherichia coli. Arch Biochem Biophys (1969) 2.29
Mutants of Salmonella typhimurium and Escherichia coli pleiotropically defective in active transport. Proc Natl Acad Sci U S A (1972) 2.22
Beta-galactoside transport in bacterial membrane preparations: energy coupling via membrane-bounded D-lactic dehydrogenase. Proc Natl Acad Sci U S A (1970) 2.22
The electrochemical proton gradient in Escherichia coli membrane vesicles. Biochemistry (1977) 2.08
Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange, and counterflow. Biochemistry (1979) 2.07
Use of a lipophilic cation for determination of membrane potential in neuroblastoma-glioma hybrid cell suspensions. Proc Natl Acad Sci U S A (1979) 2.06
NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain. Biochemistry (1987) 2.04
The use of flow dialysis for determinations of deltapH and active transport. Methods Enzymol (1979) 1.94
Membrane potential changes during mitogenic stimulation of mouse spleen lymphocytes. Proc Natl Acad Sci U S A (1980) 1.90
A phospholipid acts as a chaperone in assembly of a membrane transport protein. J Biol Chem (1996) 1.89
Anaerobic transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A (1973) 1.88
Molecular biology and energetics of membrane transport. J Cell Physiol (1976) 1.86
Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 2. Effect of imposed delata psi, delta pH, and Delta mu H+. Biochemistry (1979) 1.85
Quantitative association between electrical potential across the cytoplasmic membrane and early gentamicin uptake and killing in Staphylococcus aureus. J Bacteriol (1984) 1.84
Regulation of sugar transport in isolated bacterial membrane preparations from Escherichia coli. Proc Natl Acad Sci U S A (1969) 1.82
Climate change and the integrity of science. Science (2010) 1.80
Electrochemical proton gradient in inverted membrane vesicles from Escherichia coli. Biochemistry (1980) 1.78
Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli. Protein Sci (1993) 1.78
Construction of a functional lactose permease devoid of cysteine residues. Biochemistry (1991) 1.77
Glycine uptake in Escherichia coli. II. Glycine uptake, exchange, and metabolism by an isolated membrane preparation. J Biol Chem (1968) 1.76
Sodium-dependent methyl 1-thio-beta-D-galactopyranoside transport in membrane vesicles isolated from Salmonella typhimurium. Biochemistry (1977) 1.75
Preparation, characterization, and properties of monoclonal antibodies against the lac carrier protein from Escherichia coli. Proc Natl Acad Sci U S A (1982) 1.74
Mechanisms of active transport in isolated membrane vesicles. IV. Galactose transport by isolated membrane vesicles from Escherichia coli. J Biol Chem (1972) 1.74
Mechanisms of active transport in isolated bacterial membrane vesicles. Further studies on amino acid transport in Staphylococcus aureus membrane vesicles. J Biol Chem (1974) 1.73
Active transport in Escherichia coli: passage to permease. Annu Rev Biophys Biophys Chem (1986) 1.72
In vivo expression of the lacY gene in two segments leads to functional lac permease. Proc Natl Acad Sci U S A (1990) 1.71
Macrophage membrane potential changes associated with gamma 2b/gamma 1 Fc receptor-ligand binding. Proc Natl Acad Sci U S A (1983) 1.70
Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli. Proc Natl Acad Sci U S A (1994) 1.68
Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes. Proc Natl Acad Sci U S A (1984) 1.68
Molecular biology of active transport: from membrane to molecule to mechanism. Harvey Lect (1989) 1.65
Mouse macrophage Fc receptor for IgG gamma 2b/gamma 1 in artificial and plasma membrane vesicles functions as a ligand-dependent ionophore. Proc Natl Acad Sci U S A (1983) 1.64
Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel. Proc Natl Acad Sci U S A (1998) 1.62
Membrane potential in anaerobically growing Staphylococcus aureus and its relationship to gentamicin uptake. Antimicrob Agents Chemother (1983) 1.62
Reconstitution of active transport in proteoliposomes containing cytochrome o oxidase and lac carrier protein purified from Escherichia coli. Proc Natl Acad Sci U S A (1983) 1.60
Active transport in membrane vesicles from Escherichia coli: the electrochemical proton gradient alters the distribution of the lac carrier between two different kinetic states. Biochemistry (1980) 1.60
Molecular structure of membrane vesicles from Escherichia coli. Proc Natl Acad Sci U S A (1978) 1.60
Mechanisms of active transport in isolated bacterial membrane vesicles. XV. Purification and properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli. J Biol Chem (1973) 1.60
Monoclonal antibodies against the lac carrier protein from Escherichia coli. 2. Binding studies with membrane vesicles and proteoliposomes reconstituted with purified lac carrier protein. Biochemistry (1984) 1.54
Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli. Biochemistry (1993) 1.54
Equilibrium between two forms of the lac carrier protein in energized and nonenergized membrane vesicles from Escherichia coli. Biochemistry (1976) 1.54
Glycine uptake in Escherichia coli. I. Glycine uptake by whole cells of Escherichia coli W+ and a D-serine-resistant. J Biol Chem (1968) 1.54
Energy-dependent binding of dansylgalactosides to the beta-galactoside carrier protein. J Biol Chem (1975) 1.49
Topology of the lac carrier protein in the membrane of Escherichia coli. Proc Natl Acad Sci U S A (1983) 1.48
cys154 Is important for lac permease activity in Escherichia coli. Biochem Biophys Res Commun (1985) 1.48
Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli. Biochemistry (1999) 1.47
pH-dependent changes in proton:substrate stoichiometries during active transport in Escherichia coli membrane vesicles. Biochemistry (1977) 1.47
Active transport in isolated bacterial membrane vesicles. V. The transport of amino acids by membrane vesicles prepared from Staphylococcus aureus. J Biol Chem (1972) 1.47
The role of phosphatidylglycerol in the vectorial phosphorylation of sugar by isolated bacterial membrane preparations. Proc Natl Acad Sci U S A (1970) 1.46
lac permease of Escherichia coli: histidine-322 and glutamic acid-325 may be components of a charge-relay system. Biochemistry (1986) 1.46
Cysteine 148 in the lactose permease of Escherichia coli is a component of a substrate binding site. 2. Site-directed fluorescence studies. Biochemistry (1994) 1.44
Role of the charge pair aspartic acid-237-lysine-358 in the lactose permease of Escherichia coli. Biochemistry (1993) 1.44
Extracellular ATP perturbs transmembrane ion fluxes, elevates cytosolic [Ca2+], and inhibits phagocytosis in mouse macrophages. J Biol Chem (1985) 1.43
Properties and purification of an active biotinylated lactose permease from Escherichia coli. Proc Natl Acad Sci U S A (1993) 1.43
Characterization of site-directed mutants in the lac permease of Escherichia coli. 2. Glutamate-325 replacements. Biochemistry (1989) 1.42
Antigenic architecture of membrane vesicles from Escherichia coli. Biochemistry (1979) 1.40
Purified reconstituted lac carrier protein from Escherichia coli is fully functional. Proc Natl Acad Sci U S A (1984) 1.40
Probing the conformation of the lactose permease of Escherichia coli by in situ site-directed sulfhydryl modification. Biochemistry (1996) 1.39
Mechanisms of active transport in isolated bacterial membrane vesicles. XII. Active transport by a mutant of Escherichia coli uncoupled for oxidative phosphorylation. Arch Biochem Biophys (1973) 1.39
Identification of the epitope for monoclonal antibody 4B1 which uncouples lactose and proton translocation in the lactose permease of Escherichia coli. Biochemistry (1996) 1.38
Functional interactions between putative intramembrane charged residues in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (1992) 1.37
Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. 1. Effect of pH and imposed membrane potential on efflux, exchange, and counterflow. Biochemistry (1983) 1.37
Mechanisms of active transport in isolated bacterial membrane vesicles. IX. The kinetics and specificity of amino acid transport in Staphylococcus aureus membrane vesicles. J Biol Chem (1972) 1.36
Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteins. Proc Natl Acad Sci U S A (1999) 1.35
Purified lac permease and cytochrome o oxidase are functional as monomers. J Biol Chem (1987) 1.35
Functional and immunochemical characterization of a mutant of Escherichia coli energy uncoupled for lactose transport. Biochemistry (1985) 1.34
Effect of diethylpyrocarbonate on lactose/proton symport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A (1979) 1.34
A five-residue sequence near the carboxyl terminus of the polytopic membrane protein lac permease is required for stability within the membrane. Proc Natl Acad Sci U S A (1989) 1.33
Cysteine-scanning mutagenesis of helix IV and the adjoining loops in the lactose permease of Escherichia coli: Glu126 and Arg144 are essential. off. Biochemistry (1997) 1.33
A general method for determining helix packing in membrane proteins in situ: helices I and II are close to helix VII in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (1996) 1.33
Role of proline residues in the structure and function of a membrane transport protein. Biochemistry (1991) 1.32
Immunochemical analysis of membrane vesicles from Escherichia coli. Biochemistry (1979) 1.31
Cytochrome o type oxidase from Escherichia coli. Characterization of the enzyme and mechanism of electrochemical proton gradient generation. Biochemistry (1984) 1.30
Exchange, efflux, and substrate binding by cysteine mutants of the lactose permease of Escherichia coli. Biochemistry (1993) 1.30
Monoclonal antibodies against the lac carrier protein from Escherichia coli. 1. Functional studies. Biochemistry (1984) 1.30
Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helices IV and V that contain the major determinants for substrate binding. Biochemistry (2001) 1.29
Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. 2. Deuterium solvent isotope effects. Biochemistry (1983) 1.28