Two heads of myosin are better than one for generating force and motion.

PubWeight™: 1.53‹?› | Rank: Top 4%

🔗 View Article (PMC 16344)

Published in Proc Natl Acad Sci U S A on April 13, 1999

Authors

M J Tyska1, D E Dupuis, W H Guilford, J B Patlak, G S Waller, K M Trybus, D M Warshaw, S Lowey

Author Affiliations

1: Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA.

Articles citing this

Stretchy proteins on stretchy substrates: the important elements of integrin-mediated rigidity sensing. Dev Cell (2010) 2.65

Mechanics of actomyosin bonds in different nucleotide states are tuned to muscle contraction. Proc Natl Acad Sci U S A (2006) 2.25

Myosin V exhibits a high duty cycle and large unitary displacement. J Cell Biol (2001) 1.95

Force generation in single conventional actomyosin complexes under high dynamic load. Biophys J (2005) 1.90

Kinetic differences at the single molecule level account for the functional diversity of rabbit cardiac myosin isoforms. J Physiol (1999) 1.43

Hidden-Markov methods for the analysis of single-molecule actomyosin displacement data: the variance-Hidden-Markov method. Biophys J (2001) 1.36

A mutant heterodimeric myosin with one inactive head generates maximal displacement. J Cell Biol (2003) 1.26

Tumor cell migration in complex microenvironments. Cell Mol Life Sci (2012) 1.16

Single-myosin crossbridge interactions with actin filaments regulated by troponin-tropomyosin. Proc Natl Acad Sci U S A (2005) 1.16

Quantifying DNA melting transitions using single-molecule force spectroscopy. J Phys Condens Matter (2009) 1.15

A point mutation in the regulatory light chain reduces the step size of skeletal muscle myosin. Proc Natl Acad Sci U S A (2004) 1.15

Actomyosin-ADP states, interhead cooperativity, and the force-velocity relation of skeletal muscle. Biophys J (2010) 1.14

Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions. PLoS One (2010) 1.13

Thick filament length and isoform composition determine self-organized contractile units in actomyosin bundles. Biophys J (2013) 1.06

The mechanism of the resistance to stretch of isometrically contracting single muscle fibres. J Physiol (2009) 1.06

Coupled myosin VI motors facilitate unidirectional movement on an F-actin network. J Cell Biol (2009) 1.06

Kinetic characterization of nonmuscle myosin IIb at the single molecule level. J Biol Chem (2012) 1.02

Phosphorylation of a single head of smooth muscle myosin activates the whole molecule. Biochemistry (2006) 0.97

Smooth muscle heavy meromyosin phosphorylated on one of its two heads supports force and motion. J Biol Chem (2009) 0.97

Biophysics of substrate interaction: influence on neural motility, differentiation, and repair. Dev Neurobiol (2011) 0.94

Coiled-coil unwinding at the smooth muscle myosin head-rod junction is required for optimal mechanical performance. Biophys J (2001) 0.90

Mouse macrophages completely lacking Rho subfamily GTPases (RhoA, RhoB, and RhoC) have severe lamellipodial retraction defects, but robust chemotactic navigation and altered motility. J Biol Chem (2014) 0.88

The contributions of filaments and cross-bridges to sarcomere compliance in skeletal muscle. J Physiol (2014) 0.88

The energetics of allosteric regulation of ADP release from myosin heads. Phys Chem Chem Phys (2009) 0.87

Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte. Biophys J (2014) 0.86

Using fluorescent myosin to directly visualize cooperative activation of thin filaments. J Biol Chem (2014) 0.85

Force-producing ADP state of myosin bound to actin. Proc Natl Acad Sci U S A (2016) 0.84

Direct observation of phosphate inhibiting the force-generating capacity of a miniensemble of Myosin molecules. Biophys J (2013) 0.83

Structural changes in isometrically contracting insect flight muscle trapped following a mechanical perturbation. PLoS One (2012) 0.83

Nonlinear cross-bridge elasticity and post-power-stroke events in fast skeletal muscle actomyosin. Biophys J (2013) 0.83

Integrin Molecular Tension within Motile Focal Adhesions. Biophys J (2015) 0.83

Reconciling the working strokes of a single head of skeletal muscle myosin estimated from laser-trap experiments and crystal structures. Proc Natl Acad Sci U S A (2006) 0.81

Direct observation of tropomyosin binding to actin filaments. Cytoskeleton (Hoboken) (2015) 0.81

The kinetics underlying the velocity of smooth muscle myosin filament sliding on actin filaments in vitro. J Biol Chem (2014) 0.80

Ultraslow myosin molecular motors of placental contractile stem villi in humans. PLoS One (2014) 0.79

Loop 2 of myosin is a force-dependent inhibitor of the rigor bond. J Muscle Res Cell Motil (2014) 0.79

How single molecule detection measures the dynamic actions of life. HFSP J (2007) 0.79

Modeling smooth muscle myosin's two heads: long-lived enzymatic roles and phosphorylation-dependent equilibria. Biophys J (2010) 0.79

Poorly understood aspects of striated muscle contraction. Biomed Res Int (2015) 0.78

Magnesium modulates actin binding and ADP release in myosin motors. J Biol Chem (2014) 0.77

Antagonistic Behaviors of NMY-1 and NMY-2 Maintain Ring Channels in the C. elegans Gonad. Biophys J (2016) 0.77

Mechanical characterization of one-headed myosin-V using optical tweezers. PLoS One (2010) 0.77

Gene transfer, expression, and sarcomeric incorporation of a headless myosin molecule in cardiac myocytes: evidence for a reserve in myofilament motor function. Am J Physiol Heart Circ Physiol (2010) 0.76

Statistical Mechanics of the Human Placenta: A Stationary State of a Near-Equilibrium System in a Linear Regime. PLoS One (2015) 0.76

Fluctuation of actin sliding over myosin thick filaments in vitro. Biophysics (Nagoya-shi) (2005) 0.75

Longitudinal distortions and transversal fluctuations of an actin filament sliding on Myosin molecules. J Biol Phys (2002) 0.75

Actomyosin interaction at low ATP concentrations. Eur Biophys J (2016) 0.75

Articles cited by this

Muscle structure and theories of contraction. Prog Biophys Biophys Chem (1957) 32.09

Proposed mechanism of force generation in striated muscle. Nature (1971) 21.45

Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature (1994) 13.73

Movement and force produced by a single myosin head. Nature (1995) 4.92

Cross-bridge model of muscle contraction. Quantitative analysis. Biophys J (1980) 4.91

Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin. Cell (1998) 3.79

Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins. J Biol Chem (1990) 3.48

Processivity of the motor protein kinesin requires two heads. J Cell Biol (1998) 3.36

Substructure of the myosin molecule. IV. Interactions of myosin and its subfragments with adenosine triphosphate and F-actin. J Mol Biol (1973) 3.07

Actomyosin interaction in striated muscle. Physiol Rev (1997) 2.52

Sliding movement of single actin filaments on one-headed myosin filaments. Nature (1987) 2.34

The stiffness of rabbit skeletal actomyosin cross-bridges determined with an optical tweezers transducer. Biophys J (1998) 2.32

Smooth muscle and skeletal muscle myosins produce similar unitary forces and displacements in the laser trap. Biophys J (1997) 2.28

Smooth and skeletal muscle myosin both exhibit low duty cycles at zero load in vitro. J Biol Chem (1993) 2.27

Actin filament mechanics in the laser trap. J Muscle Res Cell Motil (1997) 1.97

Spare the rod, spoil the regulation: necessity for a myosin rod. Proc Natl Acad Sci U S A (1997) 1.94

Detection of single-molecule interactions using correlated thermal diffusion. Proc Natl Acad Sci U S A (1997) 1.87

The movement of kinesin along microtubules. Annu Rev Physiol (1996) 1.84

Conservation within the myosin motor domain: implications for structure and function. Structure (1996) 1.73

Measuring kinetics of complex single ion channel data using mean-variance histograms. Biophys J (1993) 1.70

Electron microscopy of thin filaments decorated with a Ca2+-regulated myosin. J Mol Biol (1980) 1.64

A 7-amino-acid insert in the heavy chain nucleotide binding loop alters the kinetics of smooth muscle myosin in the laser trap. J Muscle Res Cell Motil (1998) 1.61

Two heads are required for phosphorylation-dependent regulation of smooth muscle myosin. J Biol Chem (1995) 1.52

Substructure of the myosin molecule as visualized by electron microscopy. Proc Natl Acad Sci U S A (1967) 1.48

Interaction of myosin subfragments with F-actin. Biochemistry (1978) 1.43

Regulation of expressed truncated smooth muscle myosins. Role of the essential light chain and tail length. J Biol Chem (1994) 1.35

Substructure of the myosin molecule. 3. Preparation of single-headed derivatives of myosin. J Mol Biol (1973) 1.33

Orientation dependence of displacements by a single one-headed myosin relative to the actin filament. Biophys J (1998) 1.22

One-headed kinesin derivatives move by a nonprocessive, low-duty ratio mechanism unlike that of two-headed kinesin. Biochemistry (1998) 1.16

Cooperativity between the two heads of rabbit skeletal muscle heavy meromyosin in binding to actin. Biophys J (1998) 1.15

Heavy meromyosin binds actin with negative cooperativity. Biochemistry (1978) 1.11

Single-headed myosin II acts as a dominant negative mutation in Dictyostelium. Proc Natl Acad Sci U S A (1995) 1.10

Generation of force by single-headed myosin. J Mol Biol (1978) 1.09

Leading the procession: new insights into kinesin motors. J Cell Biol (1998) 1.02

Catalytic consequences of oligomeric organization: kinetic evidence for "tethered" acto-heavy meromyosin at low ATP concentrations. Proc Natl Acad Sci U S A (1984) 0.99

Dynamic behaviour of the head-tail junction of myosin. J Mol Biol (1996) 0.92

Single-headed scallop myosin and regulation. J Biol Chem (1996) 0.83

Articles by these authors

Quantitative comparison of algorithms for tracking single fluorescent particles. Biophys J (2001) 7.71

Substructure of the myosin molecule. I. Subfragments of myosin by enzymic degradation. J Mol Biol (1969) 6.44

Preparation of myosin and its subfragments from rabbit skeletal muscle. Methods Enzymol (1982) 6.29

Calcium channels, potassium channels, and voltage dependence of arterial smooth muscle tone. Am J Physiol (1990) 4.71

Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state. Cell (1998) 4.64

Substructure of the myosin molecule. IV. Interactions of myosin and its subfragments with adenosine triphosphate and F-actin. J Mol Biol (1973) 3.07

Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2. Proc Natl Acad Sci U S A (2001) 2.93

Kinetic studies of the cooperative binding of subfragment 1 to regulated actin. Proc Natl Acad Sci U S A (1980) 2.74

Single glutamate-activated channels in locust muscle. Nature (1979) 2.65

Slow currents through single sodium channels of the adult rat heart. J Gen Physiol (1985) 2.58

A bent monomeric conformation of myosin from smooth muscle. Proc Natl Acad Sci U S A (1982) 2.51

Smooth muscle myosin cross-bridge interactions modulate actin filament sliding velocity in vitro. J Cell Biol (1990) 2.51

Molecular mechanics of cardiac myosin-binding protein C in native thick filaments. Science (2012) 2.48

Functional coupling of ryanodine receptors to KCa channels in smooth muscle cells from rat cerebral arteries. J Gen Physiol (1999) 2.42

Single-molecule mechanics of R403Q cardiac myosin isolated from the mouse model of familial hypertrophic cardiomyopathy. Circ Res (2000) 2.35

Conformational states of smooth muscle myosin. Effects of light chain phosphorylation and ionic strength. J Biol Chem (1984) 2.29

Skeletal muscle myosin light chains are essential for physiological speeds of shortening. Nature (1993) 2.29

Smooth muscle and skeletal muscle myosins produce similar unitary forces and displacements in the laser trap. Biophys J (1997) 2.28

Smooth and skeletal muscle myosin both exhibit low duty cycles at zero load in vitro. J Biol Chem (1993) 2.27

Light chains from fast and slow muscle myosins. Nature (1971) 2.25

Substructure of the myosin molecule. II. The light chains of myosin. J Mol Biol (1971) 2.12

Myosin conformational states determined by single fluorophore polarization. Proc Natl Acad Sci U S A (1998) 2.07

Two modes of gating during late Na+ channel currents in frog sartorius muscle. J Gen Physiol (1986) 2.04

Actin filament mechanics in the laser trap. J Muscle Res Cell Motil (1997) 1.97

Evidence for cleft closure in actomyosin upon ADP release. Nat Struct Biol (2000) 1.97

Myosin V exhibits a high duty cycle and large unitary displacement. J Cell Biol (2001) 1.95

Distribution of myosin isoenzymes among skeletal muscle fiber types. J Cell Biol (1979) 1.92

Ca2+ currents in cerebral artery smooth muscle cells of rat at physiological Ca2+ concentrations. J Gen Physiol (1996) 1.90

Smooth, cardiac and skeletal muscle myosin force and motion generation assessed by cross-bridge mechanical interactions in vitro. J Muscle Res Cell Motil (1994) 1.85

Hypertrophic and dilated cardiomyopathy mutations differentially affect the molecular force generation of mouse alpha-cardiac myosin in the laser trap assay. Am J Physiol Heart Circ Physiol (2007) 1.82

Transient kinetics of adenosine 5'-diphosphate and adenosine 5'-(beta, gamma-imidotriphosphate) binding to subfragment 1 and actosubfragment 1. Biochemistry (1982) 1.77

Charge replacement near the phosphorylatable serine of the myosin regulatory light chain mimics aspects of phosphorylation. Proc Natl Acad Sci U S A (1994) 1.67

Computer-assisted tracking of actin filament motility. Anal Biochem (1992) 1.65

Monoclonal antibodies localize changes on myosin heavy chain isozymes during avian myogenesis. Cell (1983) 1.65

Distribution and properties of myosin isozymes in developing avian and mammalian skeletal muscle fibers. J Cell Biol (1982) 1.64

The light chain binding domain of expressed smooth muscle heavy meromyosin acts as a mechanical lever. J Biol Chem (2000) 1.64

Stereoselective actions of thiadiazinones on canine cardiac myocytes and myofilaments. Circ Res (1993) 1.61

A 7-amino-acid insert in the heavy chain nucleotide binding loop alters the kinetics of smooth muscle myosin in the laser trap. J Muscle Res Cell Motil (1998) 1.61

Genome-wide evaluation and discovery of vertebrate A-to-I RNA editing sites. Biochem Biophys Res Commun (2011) 1.55

The essential light chain is required for full force production by skeletal muscle myosin. Proc Natl Acad Sci U S A (1994) 1.52

Cardiac V1 and V3 myosins differ in their hydrolytic and mechanical activities in vitro. Circ Res (1995) 1.52

Substructure of the myosin molecule as visualized by electron microscopy. Proc Natl Acad Sci U S A (1967) 1.48

Function of skeletal muscle myosin heavy and light chain isoforms by an in vitro motility assay. J Biol Chem (1993) 1.47

Opentime heterogeneity during bursting of sodium channels in frog skeletal muscle. Biophys J (1986) 1.46

R403Q and L908V mutant beta-cardiac myosin from patients with familial hypertrophic cardiomyopathy exhibit enhanced mechanical performance at the single molecule level. J Muscle Res Cell Motil (2000) 1.44

Kinetic differences at the single molecule level account for the functional diversity of rabbit cardiac myosin isoforms. J Physiol (1999) 1.43

Interaction of myosin subfragments with F-actin. Biochemistry (1978) 1.43

Mechanical properties and myosin light chain composition of skinned muscle fibres from adult and new-born rabbits. J Physiol (1981) 1.42

Fast and slow myosin in developing muscle fibres. Nature (1978) 1.38

Polymorphism of myosin among skeletal muscle fiber types. J Cell Biol (1977) 1.36

Substructure of the myosin molecule. 3. Preparation of single-headed derivatives of myosin. J Mol Biol (1973) 1.33

Visualization of head-head interactions in the inhibited state of smooth muscle myosin. J Cell Biol (1999) 1.31

The active tension-length curve of vascular smooth muscle related to its cellular components. J Gen Physiol (1979) 1.28

Cross-bridge elasticity in single smooth muscle cells. J Gen Physiol (1983) 1.28

Probing myosin head structure with monoclonal antibodies. J Mol Biol (1986) 1.27

Enhanced force generation by smooth muscle myosin in vitro. Proc Natl Acad Sci U S A (1994) 1.27

Optical rotatory dispersion studies of rabbit gamma-G-immunoglobulin and its papain fragments. J Biol Chem (1966) 1.22

Single calcium channel currents of arterial smooth muscle at physiological calcium concentrations. Am J Physiol (1992) 1.22

Mechanism of smooth muscle myosin phosphorylation. J Biol Chem (1985) 1.21

Two conserved lysines at the 50/20-kDa junction of myosin are necessary for triggering actin activation. J Biol Chem (2000) 1.21

Functional consequences of mutations in the smooth muscle myosin heavy chain at sites implicated in familial hypertrophic cardiomyopathy. J Biol Chem (2000) 1.20

M-protein from chicken pectoralis muscle: isolation and characterization. J Mol Biol (1977) 1.20

Equilibrium binding of adenosine diphosphate to myosin. Biochemistry (1969) 1.17

Chimeric substitutions of the actin-binding loop activate dephosphorylated but not phosphorylated smooth muscle heavy meromyosin. J Biol Chem (1995) 1.16

Effects of MgATP, MgADP, and Pi on actin movement by smooth muscle myosin. J Biol Chem (1991) 1.16

Assembly of smooth muscle myosin minifilaments: effects of phosphorylation and nucleotide binding. J Cell Biol (1987) 1.16

Orientation changes of fluorescent probes at five sites on the myosin regulatory light chain during contraction of single skeletal muscle fibres. J Mol Biol (1998) 1.16

An insert in the motor domain determines the functional properties of expressed smooth muscle myosin isoforms. J Muscle Res Cell Motil (1997) 1.15

An immunochemical approach to the structure of myosin and the thick filament. J Mol Biol (1972) 1.15

Coupling of ATPase activity and motility in smooth muscle myosin is mediated by the regulatory light chain. J Cell Biol (1994) 1.14

The regulatory light chain is required for folding of smooth muscle myosin. J Biol Chem (1988) 1.13

Proteolytic degradation of myosin and the meromyosins by a water-insoluble polyanionic derivative of trypsin: properties of a helical subunit isolated from heavy meromyosin. J Mol Biol (1967) 1.09

Polymerization of myosin from smooth muscle of the calf aorta. Biochemistry (1981) 1.09

Tropomyosin directly modulates actomyosin mechanical performance at the level of a single actin filament. Proc Natl Acad Sci U S A (1999) 1.07

Mechanical and morphological properties of arterial resistance vessels in young and old spontaneously hypertensive rats. Circ Res (1979) 1.07

Smooth muscle myosin heavy chain isoforms and their role in muscle physiology. Microsc Res Tech (2000) 1.06

Loop I can modulate ADP affinity, ATPase activity, and motility of different scallop myosins. Transient kinetic analysis of S1 isoforms. Biochemistry (1998) 1.06

A minimal motor domain from chicken skeletal muscle myosin. J Biol Chem (1995) 1.05

Assembly of myosin. J Mol Biol (1971) 1.05

Myosin isozymes in avian skeletal muscles. I. Sequential expression of myosin isozymes in developing chicken pectoralis muscles. J Muscle Res Cell Motil (1983) 1.04