Quantitative comparison of algorithms for tracking single fluorescent particles.

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Published in Biophys J on October 01, 2001

Authors

M K Cheezum1, W F Walker, W H Guilford

Author Affiliations

1: Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA.

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Adrenal response to cardiac surgery. Proc R Soc Med (1965) 0.76

A Disordered Eating Response Team's Effect on Nutrition Practices in College Athletes. J Athl Train (1995) 0.76

Acceptable level of aerobic power for patients undergoing elective surgery [proceedings]. J Physiol (1978) 0.76

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Changes in human colonic mucosal-submucosal blood flow after body surface cooling. Gut (1981) 0.75

Surgical intensive care. Ann R Coll Surg Engl (1973) 0.75

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EIGHTY-SIX CITIES STUDIED BY OBJECTIVE STANDARDS. Am J Public Health (N Y) (1925) 0.75

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Annual Health Department Reports : Report of the Sub-Committee. Am J Public Health Nations Health (1930) 0.75

Sibling incest. Child Abuse Negl (1988) 0.75

CURRENT IMMUNIZATION PRACTICE IN DIPHTHERIA, SCARLET FEVER AND MEASLES. Am J Public Health (N Y) (1926) 0.75

Symposium on safe surgery. The maintenace of homeostasis. Acid-base balance. Br J Surg (1967) 0.75

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Relations Between Health Departments and Hospitals. Am J Public Health Nations Health (1928) 0.75

Tissue pH measurement [proceedings]. Biorheology (1978) 0.75

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Letter: Juvenile hormone synergists: a possible case of hasty conclusion? Science (1974) 0.75

Atherosclerosis. Physiotherapy (1972) 0.75

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Further experience in the healing rate of lower limb amputations. Eur J Vasc Surg (1988) 0.75

Administrative Problems in a Delivery Nursing Service. Am J Public Health Nations Health (1939) 0.75

Nutrition after injury. World Rev Nutr Diet (1974) 0.75

Cirsoid aneurysm of the scalp. J R Coll Surg Edinb (1982) 0.75

Effects of vaporous cavitation near prosthetic surfaces. Trans Am Soc Artif Intern Organs (1981) 0.75

Current status of thermography in peripheral vascular disease. J Cardiovasc Surg (Torino) (1976) 0.75

Rôle of Vital Statistics in Medical Science. Am J Public Health Nations Health (1937) 0.75

Arterial systolic pressures in critical ischemia. Ann Vasc Surg (1986) 0.75

Acid-base problems in surgery. World J Surg (1983) 0.75

Tissue viability--methods of measurement [proceedings]. Biorheology (1978) 0.75

Cellular and molecular studies in muscle and cultures from patients with multiple mitochondrial DNA deletions. J Neurol Sci (1999) 0.75

In vitro evaluation of U.S. and U.S.S.R. artificial hearts. Artif Organs (1982) 0.75

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The New Appraisal Form for Local Health Work. Am J Public Health Nations Health (1939) 0.75

pO2 and pH micro-electrode studies in surgical patients [proceedings]. Arzneimittelforschung (1978) 0.75

Metabolism after surgery in the elderly. Proc Nutr Soc (1971) 0.75

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