Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.

PubWeight™: 1.58‹?› | Rank: Top 4%

🔗 View Article (PMC 93859)

Published in J Bacteriol on June 01, 1999

Authors

T Asai1, C Condon, J Voulgaris, D Zaporojets, B Shen, M Al-Omar, C Squires, C L Squires

Author Affiliations

1: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

Articles citing this

Nonoptimal microbial response to antibiotics underlies suppressive drug interactions. Cell (2009) 2.27

Genome-scale reconstruction of Escherichia coli's transcriptional and translational machinery: a knowledge base, its mathematical formulation, and its functional characterization. PLoS Comput Biol (2009) 2.14

Compartmentalization of transcription and translation in Bacillus subtilis. EMBO J (2000) 1.89

Life history implications of rRNA gene copy number in Escherichia coli. Appl Environ Microbiol (2004) 1.59

Contributions of UP elements and the transcription factor FIS to expression from the seven rrn P1 promoters in Escherichia coli. J Bacteriol (2001) 1.45

Development and application of an insertional system for gene delivery and expression in Campylobacter jejuni. Appl Environ Microbiol (2005) 1.44

Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons of Escherichia coli. Nucleic Acids Res (2007) 1.40

Binding site of macrolide antibiotics on the ribosome: new resistance mutation identifies a specific interaction of ketolides with rRNA. J Bacteriol (2001) 1.33

rRNA promoter activity in the fast-growing bacterium Vibrio natriegens. J Bacteriol (2002) 1.31

Physiology of mycobacteria. Adv Microb Physiol (2009) 1.23

Growth rate regulation in Escherichia coli. FEMS Microbiol Rev (2011) 1.23

Regulation of rRNA transcription correlates with nucleoside triphosphate sensing. J Bacteriol (2001) 1.22

Evernimicin (SCH27899) inhibits a novel ribosome target site: analysis of 23S ribosomal DNA mutants. Antimicrob Agents Chemother (2000) 1.19

Binding site of the bridged macrolides in the Escherichia coli ribosome. Antimicrob Agents Chemother (2005) 1.17

The G2447A mutation does not affect ionization of a ribosomal group taking part in peptide bond formation. RNA (2003) 1.13

The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunits. RNA (2004) 1.13

A coarse-grained biophysical model of E. coli and its application to perturbation of the rRNA operon copy number. PLoS Comput Biol (2008) 1.10

Temperature sensitivity caused by mutant release factor 1 is suppressed by mutations that affect 16S rRNA maturation. J Bacteriol (2004) 1.03

Tools for characterizing bacterial protein synthesis inhibitors. Antimicrob Agents Chemother (2013) 1.01

Ribosomal RNAs are tolerant toward genetic insertions: evolutionary origin of the expansion segments. Nucleic Acids Res (2008) 0.97

Structure-activity relationships among the kanamycin aminoglycosides: role of ring I hydroxyl and amino groups. Antimicrob Agents Chemother (2012) 0.95

Negamycin binds to the wall of the nascent chain exit tunnel of the 50S ribosomal subunit. Antimicrob Agents Chemother (2007) 0.94

Helix 69 in 23S rRNA modulates decoding by wild type and suppressor tRNAs. Mol Genet Genomics (2009) 0.94

Products transcribed from rearranged rrn genes of Escherichia coli can assemble to form functional ribosomes. J Bacteriol (2003) 0.90

Role of precursor sequences in the ordered maturation of E. coli 23S ribosomal RNA. RNA (2011) 0.89

Engineered ribosomal RNA operon copy-number variants of E. coli reveal the evolutionary trade-offs shaping rRNA operon number. Nucleic Acids Res (2015) 0.88

Bases in 16S rRNA important for subunit association, tRNA binding, and translocation. Biochemistry (2009) 0.86

16S rRNA mutations that confer tetracycline resistance in Helicobacter pylori decrease drug binding in Escherichia coli ribosomes. J Bacteriol (2005) 0.85

Interaction between the ribosomal subunits: 16S rRNA suppressors of the lethal DeltaA1916 mutation in the 23S rRNA of Escherichia coli. Mol Genet Genomics (2007) 0.84

Multiple in vivo pathways for Escherichia coli small ribosomal subunit assembly occur on one pre-rRNA. Nat Struct Mol Biol (2014) 0.84

Mutational analysis of the ribosome assembly GTPase RbgA provides insight into ribosome interaction and ribosome-stimulated GTPase activation. Nucleic Acids Res (2013) 0.82

Identification of Actinobacillus suis genes essential for the colonization of the upper respiratory tract of swine. Infect Immun (2005) 0.81

Being Aquifex aeolicus: Untangling a hyperthermophile's checkered past. Genome Biol Evol (2013) 0.80

Site-directed mutants of 16S rRNA reveal important RNA domains for KsgA function and 30S subunit assembly. Biochemistry (2011) 0.78

Selection for intragenic suppressors of lethal 23S rRNA mutations in Escherichia coli identifies residues important for ribosome assembly and function. Mol Genet Genomics (2007) 0.77

Strength and Regulation of Seven rRNA Promoters in Escherichia coli. PLoS One (2015) 0.77

Functional replacement of two highly conserved tetraloops in the bacterial ribosome. Biochemistry (2012) 0.77

Decreased Expression of Stable RNA Can Alleviate the Lethality Associated with RNase E Deficiency in Escherichia coli. J Bacteriol (2017) 0.75

One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9. Sci Rep (2016) 0.75

Recurrent RNA motifs as probes for studying RNA-protein interactions in the ribosome. Nucleic Acids Res (2010) 0.75

Differential Regulation of rRNA and tRNA Transcription from the rRNA-tRNA Composite Operon in Escherichia coli. PLoS One (2016) 0.75

Articles cited by this

A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell (1976) 26.20

A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev (1989) 15.19

Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J Bacteriol (1985) 6.72

Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol Microbiol (1991) 6.44

Replacement and amplification of bacterial genes with sequences altered in vitro. Proc Natl Acad Sci U S A (1983) 6.24

Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates. J Mol Biol (1996) 5.94

Genetics of bacterial ribosomes. Annu Rev Genet (1977) 5.64

Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J Mol Biol (1990) 5.43

DNA determinants of rRNA synthesis in E. coli: growth rate dependent regulation, feedback inhibition, upstream activation, antitermination. Cell (1986) 5.08

Transcription regulation by initiating NTP concentration: rRNA synthesis in bacteria. Science (1997) 4.53

Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons. Cell (1983) 3.43

The number of rRNA genes in Escherichia coli. FEBS Lett (1977) 3.39

Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmids. Cell (1983) 3.33

Control of rRNA transcription in Escherichia coli. Microbiol Rev (1995) 3.31

Growth rate of polypeptide chains as a function of the cell growth rate in a mutant of Escherichia coli 15. J Mol Biol (1971) 3.08

rRNA operon multiplicity in Escherichia coli and the physiological implications of rrn inactivation. J Bacteriol (1995) 2.87

An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc Natl Acad Sci U S A (1999) 2.85

rRNA transcription and growth rate-dependent regulation of ribosome synthesis in Escherichia coli. Annu Rev Microbiol (1996) 2.56

Comparison of the expression of the seven ribosomal RNA operons in Escherichia coli. EMBO J (1992) 2.48

Cloning, mapping, and molecular characterization of the rRNA operons of Clostridium perfringens. J Bacteriol (1991) 2.18

Deletion of a ribosomal ribonucleic acid operon in Escherichia coli. J Bacteriol (1980) 2.10

Simple phagemid-based system for generating allele replacements in Escherichia coli. J Bacteriol (1993) 2.06

Depletion of functional ribosomal RNA operons in Escherichia coli causes increased expression of the remaining intact copies. EMBO J (1993) 1.99

Light-scattering studies showing the effect of initiation factors on the reversible dissociation of Escherichia coli ribosomes. J Mol Biol (1975) 1.96

Isolation of temperature-sensitive mutants of 16 S rRNA in Escherichia coli. J Mol Biol (1989) 1.82

Escherichia coli translation initiation factor 3 discriminates the initiation codon in vivo. Mol Microbiol (1996) 1.69

Physical map of the chromosome of Lactococcus lactis subsp. lactis DL11 and localization of six putative rRNA operons. J Bacteriol (1991) 1.43

Mapping of rRNA genes with integrable plasmids in Bacillus subtilis. J Bacteriol (1986) 1.30

Nucleotide sequence of a recA operator mutation. LexA/operator-repressor binding/inducible repair. Mol Gen Genet (1982) 0.99

Cloning and partial characterization of genes for ribosomal ribonucleic acid in Lactococcus lactis subsp. lactis. FEMS Microbiol Lett (1991) 0.91

Articles by these authors

New features of the regulation of the tryptophan operon. Science (1975) 5.40

The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene. Proc Natl Acad Sci U S A (1969) 4.57

Structural basis for signal transduction by the Toll/interleukin-1 receptor domains. Nature (2000) 4.36

Regulated in vitro synthesis of Escherichia coli tryptophan operon messenger ribonucleic acid and enzymes. J Biol Chem (1974) 4.25

Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci U S A (1980) 3.86

Nucleotide sequence of the 5' end of tryptophan messenger RNA of Escherichia coli. J Mol Biol (1976) 3.60

ClpB is the Escherichia coli heat shock protein F84.1. J Bacteriol (1991) 3.38

Conservation of the regulatory subunit for the Clp ATP-dependent protease in prokaryotes and eukaryotes. Proc Natl Acad Sci U S A (1990) 3.33

Control of rRNA transcription in Escherichia coli. Microbiol Rev (1995) 3.31

An analysis of "revertants" of a deletion mutant in the C gene of the L-arabinose gene complex in Escherichia coli B-r: isolation of initiator constitutive mutants (Ic). J Mol Biol (1969) 3.25

Nucleotide sequence of region preceding trp mRNA initiation site and its role in promoter and operator function. Proc Natl Acad Sci U S A (1976) 3.13

Ribosome-protected regions in the leader-trpE sequence of Escherichia coli tryptophan operon messenger RNA. J Mol Biol (1976) 2.93

rRNA operon multiplicity in Escherichia coli and the physiological implications of rrn inactivation. J Bacteriol (1995) 2.87

An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc Natl Acad Sci U S A (1999) 2.85

Antitermination of E. coli rRNA transcription is caused by a control region segment containing lambda nut-like sequences. Cell (1984) 2.68

Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity. Cell (1998) 2.67

Nucleotide sequences from tryptophan messenger RNA of Escherichia coli: the sequence corresponding to the amino-terminal region of the first polypeptide specified by the operon. Proc Natl Acad Sci U S A (1973) 2.58

The Clp proteins: proteolysis regulators or molecular chaperones? J Bacteriol (1992) 2.58

The biosynthetic gene cluster for the antitumor drug bleomycin from Streptomyces verticillus ATCC15003 supporting functional interactions between nonribosomal peptide synthetases and a polyketide synthase. Chem Biol (2000) 2.55

Comparison of the expression of the seven ribosomal RNA operons in Escherichia coli. EMBO J (1992) 2.48

Control features within the rplJL-rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci U S A (1979) 2.43

Glycine transfer RNA of Escherichia coli. II. Impaired GGA-recognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation. J Mol Biol (1970) 2.36

Translational coupling of the trpB and trpA genes in the Escherichia coli tryptophan operon. J Bacteriol (1984) 2.35

Saccharomyces cerevisiae RNase H(35) functions in RNA primer removal during lagging-strand DNA synthesis, most efficiently in cooperation with Rad27 nuclease. Mol Cell Biol (1999) 2.29

Cloning and characterization of a phosphopantetheinyl transferase from Streptomyces verticillus ATCC15003, the producer of the hybrid peptide-polyketide antitumor drug bleomycin. Chem Biol (2001) 2.25

Intrinsic fluorescence from DNA can be enhanced by metallic particles. Biochem Biophys Res Commun (2001) 2.25

Interaction of the trp repressor and RNA polymerase with the trp operon. J Mol Biol (1975) 2.23

Glycine transfer RNA of Escherichia coli. I. Structural genes for two glycine tRNA species. J Mol Biol (1970) 2.21

Regulation of in vitro transcription of the tryptophan operon by purified RNA polymerase in the presence of partially purified repressor and tryptophan. Nat New Biol (1973) 2.20

Transcription termination in vivo in the leader region of the tryptophan operon of Escherichia coli. J Mol Biol (1976) 2.08

Infliximab in ulcerative colitis is associated with an increased risk of postoperative complications after restorative proctocolectomy. Dis Colon Rectum (2008) 2.05

Termination of transcription in vitro in the Escherichia coli tryptophan operon leader region. J Mol Biol (1976) 1.99

Depletion of functional ribosomal RNA operons in Escherichia coli causes increased expression of the remaining intact copies. EMBO J (1993) 1.99

Ribosomal RNA operon anti-termination. Function of leader and spacer region box B-box A sequences and their conservation in diverse micro-organisms. J Mol Biol (1989) 1.98

Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC). Nucleic Acids Res (1981) 1.95

Normal and mutant glycine transfer RNAs. Nat New Biol (1971) 1.91

The highly recombinogenic bz locus lies in an unusually gene-rich region of the maize genome. Proc Natl Acad Sci U S A (2001) 1.88

Ureteroscopy for pediatric urolithiasis: an evolving first-line therapy. Urology (2005) 1.83

Nucleotide sequence of the rrnG ribosomal RNA promoter region of Escherichia coli. Nucleic Acids Res (1982) 1.80

Ribosomal RNA antitermination in vitro: requirement for Nus factors and one or more unidentified cellular components. Proc Natl Acad Sci U S A (1993) 1.76

A structural change occurs upon binding of syntaxin to SNAP-25. J Biol Chem (1997) 1.72

Nucleotide sequence of the promoter--operator region of the tryptophan operon of Escherichia coli. J Mol Biol (1978) 1.69

Malonyl-coenzyme A:acyl carrier protein acyltransferase of Streptomyces glaucescens: a possible link between fatty acid and polyketide biosynthesis. Biochemistry (1995) 1.60

The regulatory region of the divergent argECBH operon in Escherichia coli K-12. Nucleic Acids Res (1982) 1.60

Three adjacent transfer RNA genes in Escherichia coli. Proc Natl Acad Sci U S A (1973) 1.59

Evidence for antitermination in Escherichia coli RRNA transcription. J Bacteriol (1984) 1.59

Genes for production of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus are clustered with the cagA gene that encodes the C-1027 apoprotein. Antimicrob Agents Chemother (2000) 1.58

Proteins shared by the transcription and translation machines. Annu Rev Microbiol (2000) 1.57

Ribosomal protein S4 is a transcription factor with properties remarkably similar to NusA, a protein involved in both non-ribosomal and ribosomal RNA antitermination. EMBO J (2001) 1.57

The effects of liver transplantation on the clinical course of colitis in ulcerative colitis patients with primary sclerosing cholangitis. Aliment Pharmacol Ther (2012) 1.52

Mutational spectrum analysis of RNase H(35) deficient Saccharomyces cerevisiae using fluorescence-based directed termination PCR. Nucleic Acids Res (2000) 1.50

Aminoacyl-tRNA synthetase gene regulation in Bacillus subtilis: induction, repression and growth-rate regulation. Mol Microbiol (1995) 1.47

Tryptophanyl-tRNA and tryptophanyl-tRNA synthetase are not required for in vitro repression of the tryptophan operon. Nat New Biol (1973) 1.46

Diagnostic value of clonality of surface immunoglobulin light and heavy chains in malignant lymphoproliferative disorders. Am J Hematol (1993) 1.45

The DNA-binding and enhancer-blocking domains of the Drosophila suppressor of Hairy-wing protein. Mol Cell Biol (1996) 1.44

Enhancement of translation by the downstream box does not involve base pairing of mRNA with the penultimate stem sequence of 16S rRNA. Proc Natl Acad Sci U S A (1999) 1.43

Antiterminator-dependent modulation of transcription elongation rates by NusB and NusG. Mol Microbiol (1999) 1.41

Identification and characterization of an eight-cysteine repeat of the latent transforming growth factor-beta binding protein-1 that mediates bonding to the latent transforming growth factor-beta1. J Biol Chem (1996) 1.40

Diagnostic value of serum gamma-glutamyl transferase isoenzyme for hepatocellular carcinoma: a 10-year study. Am J Gastroenterol (1992) 1.37

Growth inhibition of Escherichia coli K-12 by L-valine: a consequence of a regulatory pattern. Mol Gen Genet (1977) 1.37

Human exonuclease 1 functionally complements its yeast homologues in DNA recombination, RNA primer removal, and mutation avoidance. J Biol Chem (1999) 1.36

Hybrid peptide-polyketide natural products: biosynthesis and prospects toward engineering novel molecules. Metab Eng (2001) 1.35

Molecular biology, biochemistry and bioenergetics of fumarate reductase, a complex membrane-bound iron-sulfur flavoenzyme of Escherichia coli. Biochim Biophys Acta (1985) 1.35

In vivo translation of a region within the rrnB 16S rRNA gene of Escherichia coli. J Bacteriol (1987) 1.33

Major rearrangements in the 70S ribosomal 3D structure caused by a conformational switch in 16S ribosomal RNA. EMBO J (1999) 1.32

Concomitant over-expression of vascular endothelial growth factor and its receptors in pancreatic cancer. Int J Cancer (2000) 1.30

Structural biochemistry of a type 2 RNase H: RNA primer recognition and removal during DNA replication. J Mol Biol (2001) 1.30

Isolation of kasugamycin resistant mutants in the 16 S ribosomal RNA of Escherichia coli. J Mol Biol (1999) 1.29

Anomalous photogalvanic effect of circularly polarized light incident on the two-dimensional electron gas in Al{x}Ga{1-x}N/GaN heterostructures at room temperature. Phys Rev Lett (2008) 1.25

Roles for beta(pat-3) integrins in development and function of Caenorhabditis elegans muscles and gonads. J Biol Chem (2001) 1.24

Antitermination of characterized transcriptional terminators by the Escherichia coli rrnG leader region. J Mol Biol (1990) 1.24

In vitro and in vivo secondary structure probing of the thrS leader in Bacillus subtilis. Nucleic Acids Res (1998) 1.23

Stimulation of eukaryotic flap endonuclease-1 activities by proliferating cell nuclear antigen (PCNA) is independent of its in vitro interaction via a consensus PCNA binding region. J Biol Chem (2001) 1.21

Mutagenesis of structural half-cystine residues in human thioredoxin and effects on the regulation of activity by selenodiglutathione. Biochemistry (1993) 1.20

Loss of the spacer loop sequence from the rrnB operon in the Escherichia coli K-12 subline that bears the relA1 mutation. J Bacteriol (1988) 1.20

Blockage of intermediate-conductance-Ca(2+) -activated K(+) channels inhibits progression of human endometrial cancer. Oncogene (2007) 1.17