Published in J Biomol NMR on August 01, 2000
Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins. Prog Nucl Magn Reson Spectrosc (2009) 1.57
Using codon optimization, chaperone co-expression, and rational mutagenesis for production and NMR assignments of human eIF2 alpha. J Biomol NMR (2004) 1.23
Solution structure of the complex between poxvirus-encoded CC chemokine inhibitor vCCI and human MIP-1beta. Proc Natl Acad Sci U S A (2006) 1.08
Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells. J Biomol NMR (2009) 0.92
X-ray crystallographic and solution state nuclear magnetic resonance spectroscopic investigations of NADP+ binding to ferredoxin NADP reductase from Pseudomonas aeruginosa. Biochemistry (2008) 0.91
Efficient 13C/15N double labeling of the avirulence protein AVR4 in a methanol-utilizing strain (Mut+) of Pichia pastoris. J Biomol NMR (2001) 0.86
Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase. J R Soc Interface (2009) 0.86
Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra. J Biomol NMR (2013) 0.85
Uniform isotope labeling of a eukaryotic seven-transmembrane helical protein in yeast enables high-resolution solid-state NMR studies in the lipid environment. J Biomol NMR (2011) 0.85
Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR. J Biomol NMR (2015) 0.83
Perdeuteration and methyl-selective (1)H, (13)C-labeling by using a Kluyveromyces lactis expression system. J Biomol NMR (2013) 0.80
Production and analysis of perdeuterated lipids from Pichia pastoris cells. PLoS One (2014) 0.78
Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris. J Biomol NMR (2015) 0.77
Deuterium-resistant algal cell line for D labeling of heterotrophs expresses enhanced level of Hsp60 in D2O medium. Appl Environ Microbiol (2005) 0.77
Antimalarial activity of cupredoxins: the interaction of Plasmodium merozoite surface protein 119 (MSP119) and rusticyanin. J Biol Chem (2013) 0.76
Efficient production of (2)H, (13)C, (15)N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds. Microb Cell Fact (2016) 0.75
Recent developments in solid-state magic-angle spinning, nuclear magnetic resonance of fully and significantly isotopically labelled peptides and proteins. Philos Trans R Soc Lond B Biol Sci (2004) 0.75
Stable isotope labeling of protein by Kluyveromyces lactis for NMR study. J Biomol NMR (2008) 0.75
Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J Biomol NMR (1992) 17.71
Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc Natl Acad Sci U S A (1997) 13.28
A novel NMR method for determining the interfaces of large protein-protein complexes. Nat Struct Biol (2000) 2.86
The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins. Annu Rev Biophys Biomol Struct (1998) 2.76
Proteolytic processing of the Plasmodium falciparum merozoite surface protein-1 produces a membrane-bound fragment containing two epidermal growth factor-like domains. Mol Biochem Parasitol (1991) 2.32
Selective methyl group protonation of perdeuterated proteins. J Mol Biol (1996) 2.15
Global folds of highly deuterated, methyl-protonated proteins by multidimensional NMR. Biochemistry (1997) 1.99
Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1. J Mol Biol (1999) 1.61
Deuterated organisms: cultivation and uses. Science (1966) 1.43
High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris. Biotechnology (N Y) (1994) 1.04
Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation. J Biomol NMR (1999) 0.98
Effect of deuteration on the amide proton relaxation rates in proteins. Heteronuclear NMR experiments on villin 14T. J Magn Reson B (1994) 0.89
An approach to global fold determination using limited NMR data from larger proteins selectively protonated at specific residue types. J Biomol NMR (1996) 0.88
Heterologous expression of a deuterated membrane-integrated receptor and partial deuteration in methylotrophic yeasts. J Biomol NMR (1999) 0.87
Expression of highly disulfide-bonded proteins in Pichia pastoris. Structure (1994) 0.84
Use of Pichia pastoris for expression of recombinant proteins. Methods Enzymol (1999) 0.81
NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein Eng (1998) 0.77
The plurality of long chain isoprenoid alcohols (polyprenols) from natural sources. Biochim Biophys Acta (1967) 2.07
Inhibitory and blocking monoclonal antibody epitopes on merozoite surface protein 1 of the malaria parasite Plasmodium falciparum. J Mol Biol (2001) 1.87
Intracellular calcium measurements by 19F NMR of fluorine-labeled chelators. Proc Natl Acad Sci U S A (1983) 1.77
Nuclear magnetic resonance spectrometry of naturally occurring polyprenols. Anal Biochem (1967) 1.63
Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1. J Mol Biol (1999) 1.61
Mapping the binding site for matrix metalloproteinase on the N-terminal domain of the tissue inhibitor of metalloproteinases-2 by NMR chemical shift perturbation. Biochemistry (1997) 1.50
Binding of flexible ligands to macromolecules. Nature (1975) 1.45
Ischemic sinus node hibernation: resolution following angioplasty. Am Heart J (1991) 1.43
Photolabile precursors of inositol phosphates. Preparation and properties of 1-(2-nitrophenyl)ethyl esters of myo-inositol 1,4,5-trisphosphate. Biochemistry (1989) 1.43
Frontal lobe volume in patients with Huntington's disease. Neurology (1998) 1.38
The conformation of acetylcholine and related compounds in aqueous solution as studied by nuclear magnetic resonance spectroscopy. Mol Pharmacol (1972) 1.24
Structure revision of the antibiotic echinomycin. J Am Chem Soc (1975) 1.21
Ca2+ coordination to backbone carbonyl oxygen atoms in calmodulin and other EF-hand proteins: 15N chemical shifts as probes for monitoring individual-site Ca2+ coordination. Biochemistry (1998) 1.20
Solution structure of the active domain of tissue inhibitor of metalloproteinases-2. A new member of the OB fold protein family. Biochemistry (1994) 1.18
31P-n.m.r. studies on cerebral energy metabolism under conditions of hypoglycaemia and hypoxia in vitro. Biochem J (1983) 1.13
Peroxisomal membrane proteins are properly targeted to peroxisomes in the absence of COPI- and COPII-mediated vesicular transport. J Cell Sci (2001) 1.13
Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner. Mol Biol Cell (2000) 1.12
Conformational studies of oxytocin and lysine vasopressin in aqueous solution using high resolution NMR spectroscopy. Nat New Biol (1971) 1.11
High resolution structure of the N-terminal domain of tissue inhibitor of metalloproteinases-2 and characterization of its interaction site with matrix metalloproteinase-3. J Biol Chem (1998) 1.11
Imaging of acute pyelonephritis in the adult. Eur Radiol (2006) 1.08
A kinetic study of wild-type and mutant dihydrofolate reductases from Lactobacillus casei. Biochemistry (1989) 1.06
High-resolution solution structure of human pNR-2/pS2: a single trefoil motif protein. J Mol Biol (1997) 1.06
1H-NMR studies at 500 MHz of a neutral disaccharide and sulphated di-, tetra-, hexa- and larger oligosaccharides obtained by endo-beta-galactosidase treatment of keratan sulphate. Eur J Biochem (1986) 1.04
Anti-HIV-1 activity of chemically modified heparins: correlation between binding to the V3 loop of gp120 and inhibition of cellular HIV-1 infection in vitro. Biochemistry (1994) 1.03
19 Fnuclear magnetic resonance studies of ligand binding to 3-fluorotyrosine-and 6-fluorotryptophan-containing dihydrofolate reductase from Lactobacillus casei. Biochemistry (1977) 0.97
Solution structure of a trefoil-motif-containing cell growth factor, porcine spasmolytic protein. Proc Natl Acad Sci U S A (1994) 0.96
Dihydrofolate reductase. 1H resonance assignments and coenzyme-induced conformational changes. J Mol Biol (1986) 0.96
Sexual risk behavior, alcohol use, and social media use among secondary school students in informal settlements in Cape Town and Port Elizabeth, South Africa. AIDS Behav (2014) 0.96
Structure of the B-Myb DNA-binding domain in solution and evidence for multiple conformations in the region of repeat-2 involved in DNA binding: implications for sequence-specific DNA binding by Myb proteins. Eur J Biochem (1996) 0.95
Antigenic and sequence diversity at the C-terminus of the merozoite surface protein-1 from rodent malaria isolates, and the binding of protective monoclonal antibodies. Mol Biochem Parasitol (1999) 0.95
31P NMR studies of NADPH and NADP+ binding to L. casei dihydrofolate reductase. Nature (1975) 0.93
Rubratoxin B, a proposed structure for a bis-anhydride rom Penicillium rubrum Stoll. Nature (1968) 0.93
Solution structure of the B-Myb DNA-binding domain: a possible role for conformational instability of the protein in DNA binding and control of gene expression. Biochemistry (1998) 0.92
1H nuclear magnetic resonance studies of the tyrosine residues of selectively deuterated Lactobacillus casei dihydrofolate reductase. Proc R Soc Lond B Biol Sci (1977) 0.92
Structural analysis of the O-glycosidically linked core-region oligosaccharides of human meconium glycoproteins which express oncofoetal antigens. Eur J Biochem (1985) 0.92
Use of transferred nuclear Overhauser effect measurements to compare binding of coenzyme analogues to dihydrofolate reductase. Biochemistry (1983) 0.92
Structures of N-glycans of a ricin-resistant mutant of baby hamster kidney cells. Synthesis of high-mannose and hybrid N-glycans. Biochemistry (1985) 0.92
Kinetics of cytosolic Ca2+ concentration after photolytic release of 1-D-myo-inositol 1,4-bisphosphate 5-phosphorothioate from a caged derivative in guinea pig hepatocytes. Biophys J (1995) 0.91
Properties of baby-hamster kidney (BHK) cells treated with Swainsonine, an inhibitor of glycoprotein processing. Comparison with ricin-resistant BHK-cell mutants. Biochem J (1986) 0.91
Structural comparisons of complexes of methotrexate analogues with Lactobacillus casei dihydrofolate reductase by two-dimensional 1H NMR at 500 MHz. Biochemistry (1987) 0.90
Ricin-resistant mutants of baby-hamster-kidney cells deficient in alpha-mannosidase-II-catalyzed processing of asparagine-linked oligosaccharides. Eur J Biochem (1986) 0.90
19F-n.m.r. studies of ligand binding to 5-fluorotryptophan- and 3-fluorotyrosine-containing cyclic AMP receptor protein from Escherichia coli. Biochem J (1990) 0.90
NMR approaches for monitoring domain orientations in calcium-binding proteins in solution using partial replacement of Ca2+ by Tb3+. FEBS Lett (1999) 0.90
The structure of an amino-sugar from the antibiotic vancomycin. J Chem Soc Perkin 1 (1972) 0.90
Efficiency of von Willebrand factor-mediated targeting of interleukin-8 into Weibel-Palade bodies. J Thromb Haemost (2007) 0.90
1H nuclear magnetic resonance studies of Lactobacillus casei dihydrofolate reductase: effects of substrate and inhibitor binding on the histidine residues. Proc R Soc Lond B Biol Sci (1977) 0.90
NMR solution studies of hamster galectin-3 and electron microscopic visualization of surface-adsorbed complexes: evidence for interactions between the N- and C-terminal domains. Biochemistry (2001) 0.89
Identification of the 1H resonances of valine and leucine residues in dihydrofolate reductase by using a combination of selective deuteration and two-dimensional correlation spectroscopy. FEBS Lett (1986) 0.89
31P nuclear-magnetic-resonance studies on superfused cerebral tissues. Biochem Soc Trans (1985) 0.89
Oligosaccharides obtained from a blood-group-Sd(a+) Tamm-Horsfall glycoprotein. An n.m.r. study. Biochem J (1986) 0.88
1H and 15N NMR studies of protonation and hydrogen-bonding in the binding of trimethoprim to dihydrofolate reductase. Eur Biophys J (1985) 0.88
Dynamics of trimethoprim bound to dihydrofolate reductase. Proc Natl Acad Sci U S A (1988) 0.88
Dihydrofolate reductase: multiple conformations and alternative modes of substrate binding. Biochemistry (1989) 0.87
Nuclear magnetic resonance detection of bound water molecules in the active site of Lactobacillus casei dihydrofolate reductase in aqueous solution. J Mol Biol (1992) 0.87
31P-saturation-transfer nuclear-magnetic-resonance measurements of phosphocreatine turnover in guinea-pig brain slices. Biochem J (1985) 0.87
Complete 1H-n.m.r. assignments for two core-region oligosaccharides of human meconium glycoproteins, using 1D and 2D methods at 500 MHz. Carbohydr Res (1986) 0.87
Galactosyl transferases of baby hamster kidney (BHK) cells. Characterization of two oligosaccharide products synthesised using bovine asialo submaxillary-gland mucin as acceptor. Eur J Biochem (1984) 0.86
A computerized approach to the analysis of oligosaccharide structure by high-resolution proton n.m.r. Biochem J (1984) 0.86
Characterisation by mass spectrometry and 500-MHz proton nuclear magnetic resonance spectroscopy of penta- and hexasaccharide chains of human foetal gastrointestinal mucins (meconium glycoproteins). Eur J Biochem (1989) 0.86
19F-n.m.r. studies of 3',5'-difluoromethotrexate binding to Lactobacillus casei dihydrofolate reductase. Molecular motion and coenzyme-induced conformational changes. Biochem J (1984) 0.86
Neutral oligosaccharides of bovine submaxillary mucin. A combined mass spectrometry and 1H-NMR study. Eur J Biochem (1992) 0.86
13 C NMR spectra of lecithin vesicles and erythrocyte membranes. Nature (1971) 0.86
Nuclear magnetic resonance studies of the binding of substrate analogs and coenzyme to dihydrofolate reductase from Lactobacillus casei. Biochemistry (1974) 0.86
Preparation of selectively deuterated aromatic amino acids for use in 1H NMR studies of proteins. Biochim Biophys Acta (1976) 0.85
The solution structure of the complex of Lactobacillus casei dihydrofolate reductase with methotrexate. J Mol Biol (1998) 0.85
Pharmacokinetics of continuous intravenous and subcutaneous infusions of cytosine arabinoside. Blood (1982) 0.85
Histidine residues of Lactobacillus casei dihydrofolate reductase: paramagnetic relaxation and deuterium-exchange studies and partial assignments. Biochemistry (1980) 0.85
Mortality in antiepileptic drug development programs. Neurology (2001) 0.84
Investigation of the mechanism of ligand binding with cobalt(II) human carbonic anhydrase by 1 H and 19 F nuclear magnetic resonance spectroscopy. Biochemistry (1971) 0.84
Separation of human milk oligosaccharides by recycling chromatography. First isolation of lacto-N-neo-difucohexaose II and 3'-Galactosyllactose from this source. Carbohydr Res (1988) 0.84
NMR studies of multiple conformations in complexes of Lactobacillus casei dihydrofolate reductase with analogues of pyrimethamine. Biochemistry (1990) 0.83
Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate. Protein Sci (1999) 0.83
The solution structure of the disulphide-linked homodimer of the human trefoil protein TFF1. FEBS Lett (2001) 0.83
Conformational differences between complexes of elongation factor Tu studied 19F-NMR spectroscopy. Eur J Biochem (1993) 0.83
NMR-based structural studies of the pNR-2/pS2 single domain trefoil peptide. Similarities to porcine spasmolytic peptide and evidence for a monomeric structure. Eur J Biochem (1995) 0.83
A nuclear magnetic resonance study of nicotinamide adenine dinucleotide phosphate binding to Lactobacillus casei dihydrofolate reductase. Biochemistry (1975) 0.83
Identification by n.m.r. spectroscopy of a stable intermediate structure in the unfolding of staphylococcal beta-lactamase. Biochem J (1983) 0.83
19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP receptor protein from E. coli. FEBS Lett (1991) 0.83
Effects of coenzyme binding on histidine residues of Lactobacillus casei dihydrofolate reductase. Biochemistry (1981) 0.83
Cardiac surgery in patients over the age of 80 years. Clin Cardiol (1990) 0.82
Critical care nurse perceptions of family needs. Heart Lung (1991) 0.82
Proximity of two tryptophan residues in dihydrofolate reductase determined by 19f NMR. Nature (1978) 0.82
Cyanide binding to carbonic anhydrase. A 13 C-nuclear-magnetic-resonance study. Eur J Biochem (1973) 0.82
Determination of stereospecific assignments, torsion-angle constraints, and rotamer populations in proteins using the program AngleSearch. J Magn Reson B (1995) 0.82
The influence of aspartate 26 on the tautomeric forms of folate bound to Lactobacillus casei dihydrofolate reductase. FEBS Lett (1997) 0.82
31P NMR studies of the binding of adenosine-2'-phosphate to Lactobacillus casei dihydrofolate reductase. FEBS Lett (1977) 0.81
Negative cooperativity between folinic acid and coenzyme in their binding to Lactobacillus casei dihydrofolate reductase. Biochemistry (1981) 0.81
NMR evidence for the structure of the complex between penicillin and the DD-carboxypeptidase of Streptomyces R61. FEBS Lett (1979) 0.81
Structure, biosynthesis and genetics of the Sda antigen. Biochem Soc Trans (1987) 0.81
Nuclear magnetic resonance studies of the binding of trimethoprim to dihydrofolate reductase. Biochemistry (1979) 0.81
Correlated bond rotations in interactions of arginine residues with ligand carboxylate groups in protein ligand complexes. FEBS Lett (1997) 0.81
Reassessment of the acceptor specificity and general properties of the Lewis blood-group gene associated alpha-3/4-fucosyltransferase purified from human milk. Glycoconj J (1992) 0.81
Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution. Biochemistry (1991) 0.80
Hydrogen-1 nuclear magnetic resonance study of the complexes of two diastereoisomers of folinic acid with dihydrofolate reductase. Biochemistry (1981) 0.80
A structure-activity study by nuclear magnetic resonance of peptide interactions with vancomycin. Mol Pharmacol (1975) 0.80
Proton spin-lattice relaxation studies of the histidine residues of pancreatic ribonuclease. Biochim Biophys Acta (1972) 0.80
Characterisation by mass spectrometry and 1H-NMR of novel hexasaccharides among the acidic O-linked carbohydrate chains of bovine submaxillary mucin. Eur J Biochem (1992) 0.80
Methotrexate binding to dihydrofolate reductase. Nature (1980) 0.80
A 1H n.m.r. study of the role of the glutamate moiety in the binding of methotrexate to Lactobacillus casei dihydrofolate reductase. Br J Pharmacol (1984) 0.80