The large resolvase TndX is required and sufficient for integration and excision of derivatives of the novel conjugative transposon Tn5397.

PubWeight™: 1.25‹?› | Rank: Top 10%

🔗 View Article (PMC 111396)

Published in J Bacteriol on December 01, 2000

Authors

H Wang1, P Mullany

Author Affiliations

1: Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, London WC1X 8LD, United Kingdom.

Articles citing this

Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow. Nat Rev Microbiol (2010) 3.59

Genomic and functional analyses of SXT, an integrating antibiotic resistance gene transfer element derived from Vibrio cholerae. J Bacteriol (2002) 2.71

The repertoire of ICE in prokaryotes underscores the unity, diversity, and ubiquity of conjugation. PLoS Genet (2011) 2.01

Mpi recombinase globally modulates the surface architecture of a human commensal bacterium. Proc Natl Acad Sci U S A (2003) 1.84

Revised nomenclature for transposable genetic elements. Plasmid (2008) 1.50

Synapsis and DNA cleavage in phiC31 integrase-mediated site-specific recombination. Nucleic Acids Res (2004) 1.30

A motif in the C-terminal domain of phiC31 integrase controls the directionality of recombination. Nucleic Acids Res (2008) 1.25

Comparative genome analysis of 19 Ureaplasma urealyticum and Ureaplasma parvum strains. BMC Microbiol (2012) 1.21

Sequences in attB that affect the ability of phiC31 integrase to synapse and to activate DNA cleavage. Nucleic Acids Res (2007) 1.15

Attachment site recognition and regulation of directionality by the serine integrases. Nucleic Acids Res (2013) 0.97

Roles of two large serine recombinases in mobilizing the methicillin-resistance cassette SCCmec. Mol Microbiol (2013) 0.95

Integrative and Conjugative Elements (ICEs): What They Do and How They Work. Annu Rev Genet (2015) 0.93

The conjugative transposon Tn5397 has a strong preference for integration into its Clostridium difficile target site. J Bacteriol (2006) 0.93

New transposon Tn6133 in methicillin-resistant Staphylococcus aureus ST398 contains vga(E), a novel streptogramin A, pleuromutilin, and lincosamide resistance gene. Antimicrob Agents Chemother (2011) 0.90

Demonstration of conjugative transposon (Tn5397)-mediated horizontal gene transfer between Clostridium difficile and Enterococcus faecalis. Antimicrob Agents Chemother (2010) 0.88

ICESp1116, the genetic element responsible for erm(B)-mediated, inducible resistance to erythromycin in Streptococcus pyogenes. Antimicrob Agents Chemother (2012) 0.85

Variation on a theme; an overview of the Tn916/Tn1545 family of mobile genetic elements in the oral and nasopharyngeal streptococci. Front Microbiol (2014) 0.84

A Plasmid-Borne System To Assess the Excision and Integration of Staphylococcal Cassette Chromosome mec Mediated by CcrA and CcrB. J Bacteriol (2015) 0.81

Mutational analysis of highly conserved residues in the phage phiC31 integrase reveals key amino acids necessary for the DNA recombination. PLoS One (2010) 0.80

Mobile genetic elements in Clostridium difficile and their role in genome function. Res Microbiol (2015) 0.80

Comparative Genomic Analysis of the ICESa2603 Family ICEs and Spread of erm(B)- and tet(O)-Carrying Transferable 89K-Subtype ICEs in Swine and Bovine Isolates in China. Front Microbiol (2016) 0.78

Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe. Appl Environ Microbiol (2017) 0.75

Integrative and conjugative elements and their hosts: composition, distribution and organization. Nucleic Acids Res (2017) 0.75

Articles cited by this

Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid. J Bacteriol (1981) 11.15

Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob Agents Chemother (1999) 8.36

The integrase family of site-specific recombinases: regional similarities and global diversity. EMBO J (1986) 8.12

Dynamic, structural, and regulatory aspects of lambda site-specific recombination. Annu Rev Biochem (1989) 7.25

Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene (1991) 6.10

In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc Natl Acad Sci U S A (1998) 5.04

Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse. Nature (1997) 4.39

Catalysis by site-specific recombinases. Trends Genet (1992) 3.86

Conjugative transposons: an unusual and diverse set of integrated gene transfer elements. Microbiol Rev (1995) 3.75

Suicide recombination substrates yield covalent lambda integrase-DNA complexes and lead to identification of the active site tyrosine. J Biol Chem (1988) 3.43

Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases. EMBO J (1989) 3.26

Nucleotide sequence of the 18-kb conjugative transposon Tn916 from Enterococcus faecalis. Plasmid (1994) 3.01

Site-specific recombinases: changing partners and doing the twist. J Bacteriol (1986) 2.71

Two conjugation systems associated with Streptococcus faecalis plasmid pCF10: identification of a conjugative transposon that transfers between S. faecalis and Bacillus subtilis. J Bacteriol (1987) 2.61

Homology of a transferable tetracycline resistance determinant of Clostridium difficile with Streptococcus (Enterococcus) faecalis transposon Tn916. Antimicrob Agents Chemother (1987) 2.43

A resolvase-like protein is required for the site-specific integration of the temperate lactococcal bacteriophage TP901-1. J Bacteriol (1996) 2.39

Evidence for a second conserved arginine residue in the integrase family of recombination proteins. Protein Eng (1992) 2.39

Conjugative transposition. Annu Rev Microbiol (1995) 2.30

Tn916 family conjugative transposons and dissemination of antimicrobial resistance determinants. Antimicrob Agents Chemother (1998) 2.24

Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Mol Microbiol (1994) 1.88

A functional origin of transfer (oriT) on the conjugative transposon Tn916. J Bacteriol (1995) 1.69

Swapping DNA strands and sensing homology without branch migration in lambda site-specific recombination. Curr Biol (1995) 1.69

Resolvase-mediated recombination intermediates contain a serine residue covalently linked to DNA. Cold Spring Harb Symp Quant Biol (1984) 1.66

Anabaena xisF gene encodes a developmentally regulated site-specific recombinase. Genes Dev (1994) 1.64

The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase. J Bacteriol (1990) 1.61

Genetic analysis of a tetracycline resistance element from Clostridium difficile and its conjugal transfer to and from Bacillus subtilis. J Gen Microbiol (1990) 1.57

Characterization of the left 4 kb of conjugative transposon Tn916: determinants involved in excision. Plasmid (1993) 1.57

Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res. Proc Natl Acad Sci U S A (1986) 1.52

Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase. J Bacteriol (1991) 1.47

A group II intron in a conjugative transposon from the gram-positive bacterium, Clostridium difficile. Gene (1996) 1.47

Transfer of a conjugative transposon, Tn5397 in a model oral biofilm. FEMS Microbiol Lett (1999) 1.47

Mutants of the gamma delta resolvase: a genetic analysis of the recombination function. Cell (1984) 1.38

Worldwide distribution of the conjugative Clostridium perfringens tetracycline resistance plasmid, pCW3. Plasmid (1985) 1.38

Characterization of the ends and target sites of the novel conjugative transposon Tn5397 from Clostridium difficile: excision and circularization is mediated by the large resolvase, TndX. J Bacteriol (2000) 1.27

Identification of the active site tyrosine of Flp recombinase. Possible relevance of its location to the mechanism of recombination. J Biol Chem (1990) 1.27

The resolvase/invertase domain of the site-specific recombinase TnpX is functional and recognizes a target sequence that resembles the junction of the circular form of the Clostridium perfringens transposon Tn4451. J Bacteriol (1997) 1.24

Regulation of excision of the conjugative transposon Tn916. Mol Microbiol (1999) 1.22

Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916. Nucleic Acids Res (1997) 1.18

Nucleotide sequence analysis and expression studies of a chloramphenicol-acetyltransferase-coding gene from Clostridium perfringens. Gene (1989) 1.14

The tyrosine-6 hydroxyl of gamma delta resolvase is not required for the DNA cleavage and rejoining reactions. Mol Microbiol (1995) 1.04

Length of the coupling sequence of Tn916. J Bacteriol (1994) 1.04

Mapping interactions between the catalytic domain of resolvase and its DNA substrate using cysteine-coupled EDTA-iron. Biochemistry (1993) 1.02

Binding of the Bacillus subtilis spoIVCA product to the recombination sites of the element interrupting the sigma K-encoding gene. Proc Natl Acad Sci U S A (1992) 0.98

A mutational analysis of the bacteriophage P1 cin recombinase gene: intragenic complementation. Mol Gen Genet (1989) 0.81

Articles by these authors

Molecular cloning and expression of Campylobacter pylori species-specific antigens in Escherichia coli K-12. Infect Immun (1989) 2.81

Characterization of a cell surface protein of Clostridium difficile with adhesive properties. Infect Immun (2001) 1.64

Prevalence of tetracycline resistance genes in oral bacteria. Antimicrob Agents Chemother (2003) 1.59

Expression of plasmids coding for colonization factor antigen II (CFA/II) and enterotoxin production in Escherichia coli. J Gen Microbiol (1983) 1.53

Transfer of a conjugative transposon, Tn5397 in a model oral biofilm. FEMS Microbiol Lett (1999) 1.47

Comparison of Tn5397 from Clostridium difficile, Tn916 from Enterococcus faecalis and the CW459tet(M) element from Clostridium perfringens shows that they have similar conjugation regions but different insertion and excision modules. Microbiology (2001) 1.44

Characterization of the ends and target sites of the novel conjugative transposon Tn5397 from Clostridium difficile: excision and circularization is mediated by the large resolvase, TndX. J Bacteriol (2000) 1.27

Transfer of TN916-like elements in microcosm dental plaques. Antimicrob Agents Chemother (2001) 1.19

Novel tetracycline resistance determinant from the oral metagenome. Antimicrob Agents Chemother (2003) 1.18

Molecular cloning and genetic analysis of a chloramphenicol acetyltransferase determinant from Clostridium difficile. Antimicrob Agents Chemother (1988) 1.14

Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis. J Antimicrob Chemother (2013) 1.09

Effect of amoxicillin use on oral microbiota in young children. Antimicrob Agents Chemother (2004) 1.02

Gene transfer in bacterial biofilms. Methods Enzymol (2001) 1.01

Prevalence, proportions, and identities of antibiotic-resistant bacteria in the oral microflora of healthy children. Microb Drug Resist (2003) 1.01

DNA sequence of the insertional hot spot of Tn916 in the Clostridium difficile genome and discovery of a Tn916-like element in an environmental isolate integrated in the same hot spot. FEMS Microbiol Lett (2000) 0.99

Nucleotide sequence of a chloramphenicol acetyl transferase gene from Clostridium difficile. Nucleic Acids Res (1989) 0.94

Characterization of the genetic basis of antibiotic resistance in Clostridium difficile. J Antimicrob Chemother (1994) 0.92

Hybridization analysis of three chloramphenicol resistance determinants from Clostridium perfringens and Clostridium difficile. Antimicrob Agents Chemother (1989) 0.91

Construction and characterization of a Helicobacter pylori clpB mutant and role of the gene in the stress response. J Bacteriol (1998) 0.88

Determination of the genetic support for tet(W) in oral bacteria. Antimicrob Agents Chemother (2007) 0.88

Demonstration that the group II intron from the Clostridial Conjugative transposon Tn5397 undergoes splicing In vivo. J Bacteriol (2001) 0.87

Potential role of Veillonella spp. as a reservoir of transferable tetracycline resistance in the oral cavity. Antimicrob Agents Chemother (2006) 0.87

Genetic basis of erythromycin resistance in oral bacteria. Antimicrob Agents Chemother (2004) 0.86

Penicillin and amoxicillin resistance in oral Veillonella spp. Int J Antimicrob Agents (2012) 0.86

Prevalence and antibiotic resistance profile of mercury-resistant oral bacteria from children with and without mercury amalgam fillings. J Antimicrob Chemother (2002) 0.86

Composition and antibiotic resistance profile of microcosm dental plaques before and after exposure to tetracycline. J Antimicrob Chemother (2002) 0.84

Isolation of silver- and antibiotic-resistant Enterobacter cloacae from teeth. Oral Microbiol Immunol (2005) 0.82

Prevalence and persistence of amoxycillin-resistant bacteria in the dental plaques of adults. Microbios (1999) 0.81

The transfer of bacteria to, and survival on, dental records. Microbios (1999) 0.75

Antibiotic resistance in the cultivable plaque microbiota of children from different ethnic groups. Int J Antimicrob Agents (2006) 0.75

Telephone service for residents. Nurs Mirror (1983) 0.75

Glasnost health care. Nurs Stand (1990) 0.75