Published in Biotechnol Appl Biochem on April 01, 2002
Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promoters. Appl Environ Microbiol (2007) 1.26
Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly. Microb Cell Fact (2012) 0.97
Transcriptional pathways associated with the slow growth phenotype of transformed Anaplasma marginale. BMC Genomics (2013) 0.87
A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation. Nucleic Acids Res (2005) 0.84
RNA stem-loop enhanced expression of previously non-expressible genes. Nucleic Acids Res (2004) 0.83
Learning to predict expression efficacy of vectors in recombinant protein production. BMC Bioinformatics (2010) 0.80
A directed evolution strategy for optimized export of recombinant proteins reveals critical determinants for preprotein discharge. Protein Sci (2004) 0.80
Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli. Nucleic Acids Res (2007) 0.79
Optimization of the Expression of Reteplase in Escherichia coli TOP10 Using Arabinose Promoter. Jundishapur J Nat Pharm Prod (2015) 0.77
Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector. Adv Pharm Bull (2013) 0.77
Exploitation of prokaryotic expression systems based on the salicylate-dependent control circuit encompassing nahR/P(sal)::xylS2 for biotechnological applications. Bioeng Bugs (2010) 0.76
Proteins from PHB granules. Protein Sci (2005) 0.76
Down Regulation of ackA-pta Pathway in Escherichia coli BL21 (DE3): A Step Toward Optimized Recombinant Protein Expression System. Jundishapur J Microbiol (2014) 0.75
High yield production of pigeon circovirus capsid protein in the E. coli by evaluating the key parameters needed for protein expression. BMC Vet Res (2014) 0.75
Reduction of N-terminal methionylation while increasing titer by lowering metabolic and protein production rates in E. coli auto-induced fed-batch fermentation. J Ind Microbiol Biotechnol (2012) 0.75
Tumor imaging using a picomolar affinity HER2 binding affibody molecule. Cancer Res (2006) 2.46
Consequences of membrane protein overexpression in Escherichia coli. Mol Cell Proteomics (2007) 2.11
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Stabilization of a beta-hairpin in monomeric Alzheimer's amyloid-beta peptide inhibits amyloid formation. Proc Natl Acad Sci U S A (2008) 1.99
Affibody molecules for epidermal growth factor receptor targeting in vivo: aspects of dimerization and labeling chemistry. J Nucl Med (2009) 1.54
Anti-idiotypic protein domains selected from protein A-based affibody libraries. Proteins (2002) 1.52
Display of proteins on bacteria. J Biotechnol (2002) 1.48
Structural basis for recognition by an in vitro evolved affibody. Proc Natl Acad Sci U S A (2003) 1.46
An affibody in complex with a target protein: structure and coupled folding. Proc Natl Acad Sci U S A (2003) 1.35
Directed evolution to low nanomolar affinity of a tumor-targeting epidermal growth factor receptor-binding affibody molecule. J Mol Biol (2008) 1.19
Epitope mapping of antibodies using bacterial surface display. Nat Methods (2008) 1.18
Biotechnological applications for surface-engineered bacteria. Biotechnol Appl Biochem (2004) 1.17
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues. Mol Cell Proteomics (2003) 1.15
Non-immunoglobulin based protein scaffolds. Curr Opin Biotechnol (2011) 1.14
Minimum information about a protein affinity reagent (MIAPAR). Nat Biotechnol (2010) 1.10
Selection and characterization of Affibody ligands binding to Alzheimer amyloid beta peptides. J Biotechnol (2006) 1.07
Cellular studies of binding, internalization and retention of a radiolabeled EGFR-binding affibody molecule. Nucl Med Biol (2007) 1.06
Sequestration of the Abeta peptide prevents toxicity and promotes degradation in vivo. PLoS Biol (2010) 1.05
Microbead display of proteins by cell-free expression of anchored DNA. J Biotechnol (2003) 1.04
Engineering of a femtomolar affinity binding protein to human serum albumin. Protein Eng Des Sel (2008) 1.04
In vitro characterization of a bivalent anti-HER-2 affibody with potential for radionuclide-based diagnostics. Cancer Biother Radiopharm (2005) 1.03
Affibody-mediated tumour targeting of HER-2 expressing xenografts in mice. Eur J Nucl Med Mol Imaging (2006) 1.02
Comparative in vivo evaluation of technetium and iodine labels on an anti-HER2 affibody for single-photon imaging of HER2 expression in tumors. J Nucl Med (2006) 0.99
Structure, specificity, and mode of interaction for bacterial albumin-binding modules. J Biol Chem (2001) 0.99
Engineered affinity proteins--generation and applications. J Biotechnol (2009) 0.95
Engineered affinity proteins for tumour-targeting applications. Biotechnol Appl Biochem (2009) 0.95
Inhibiting HER3-mediated tumor cell growth with affibody molecules engineered to low picomolar affinity by position-directed error-prone PCR-like diversification. PLoS One (2013) 0.94
A novel affinity protein selection system based on staphylococcal cell surface display and flow cytometry. Protein Eng Des Sel (2008) 0.93
Engineering and characterization of a bispecific HER2 x EGFR-binding affibody molecule. Biotechnol Appl Biochem (2009) 0.92
Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase. N Biotechnol (2009) 0.92
Combining phage and staphylococcal surface display for generation of ErbB3-specific Affibody molecules. Protein Eng Des Sel (2010) 0.91
Cellular effects of HER3-specific affibody molecules. PLoS One (2012) 0.91
Fine affinity discrimination by normalized fluorescence activated cell sorting in staphylococcal surface display. FEMS Microbiol Lett (2005) 0.91
Generation of tumour-necrosis-factor-alpha-specific affibody molecules capable of blocking receptor binding in vitro. Biotechnol Appl Biochem (2009) 0.91
Molecular design and optimization of 99mTc-labeled recombinant affibody molecules improves their biodistribution and imaging properties. J Nucl Med (2011) 0.89
Generation and evaluation of bispecific affibody molecules for simultaneous targeting of EGFR and HER2. Bioconjug Chem (2012) 0.87
Evaluation of staphylococcal cell surface display and flow cytometry for postselectional characterization of affinity proteins in combinatorial protein engineering applications. Appl Environ Microbiol (2007) 0.86
Approaches for systematic proteome exploration. Biomol Eng (2007) 0.85
Selection and characterization of an HIV-1 gp120-binding affibody ligand. Biotechnol Appl Biochem (2006) 0.85
Affibody molecules in protein capture microarrays: evaluation of multidomain ligands and different detection formats. J Proteome Res (2007) 0.85
Vector engineering to improve a staphylococcal surface display system. FEMS Microbiol Lett (2002) 0.85
Evaluation of a HER2-targeting affibody molecule combining an N-terminal HEHEHE-tag with a GGGC chelator for 99mTc-labelling at the C terminus. Tumour Biol (2012) 0.85
Immunisation of mice against neosporosis with recombinant NcSRS2 iscoms. Vet Parasitol (2005) 0.84
Surface display of a single-domain antibody library on Gram-positive bacteria. Cell Mol Life Sci (2012) 0.84
Engineering of a bispecific affibody molecule towards HER2 and HER3 by addition of an albumin-binding domain allows for affinity purification and in vivo half-life extension. Biotechnol J (2014) 0.84
Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A. Eur J Biochem (2002) 0.83
HER2-positive tumors imaged within 1 hour using a site-specifically 11C-labeled Sel-tagged affibody molecule. J Nucl Med (2012) 0.83
Enhancement of DNA vaccine potency by linkage of Plasmodium falciparum malarial antigen gene fused with a fragment of HSP70 gene. Vaccine (2005) 0.82
Fluorescent detection of beta-lactamase activity in living Escherichia coli cells via esterase supplementation. FEMS Microbiol Lett (2005) 0.82
Engineering streptococcal protein G for increased alkaline stability. Protein Eng (2002) 0.81
1H, 13C and 15N resonance assignments of an affibody-target complex. J Biomol NMR (2002) 0.81
Biophysical characterization of Z(SPA-1)--a phage-display selected binder to protein A. Protein Sci (2004) 0.81
An improved dual-expression concept, generating high-quality antibodies for proteomics research. Biotechnol Appl Biochem (2003) 0.80
Imaging of HER3-expressing xenografts in mice using a (99m)Tc(CO) 3-HEHEHE-Z HER3:08699 affibody molecule. Eur J Nucl Med Mol Imaging (2014) 0.80
Generation of Affibody ligands binding interleukin-2 receptor alpha/CD25. Biotechnol Appl Biochem (2008) 0.80
Engineering of staphylococcal surfaces for biotechnological applications. J Biotechnol (2002) 0.79
Affibody-mediated transferrin depletion for proteomics applications. Biotechnol J (2007) 0.79
Epitope mapping using gram-positive surface display. Curr Protoc Immunol (2010) 0.79
Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli. Nucleic Acids Res (2007) 0.79
General strategies for efficient adjuvant incorporation of recombinant subunit immunogens. Vaccine (2005) 0.79
Evaluation of different linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand. Protein Eng (2003) 0.79
Construction and characterization of affibody-Fc chimeras produced in Escherichia coli. J Immunol Methods (2002) 0.78
Simplified characterization through site-specific protease-mediated release of affinity proteins selected by staphylococcal display. FEMS Microbiol Lett (2007) 0.78
A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products. J Biotechnol (2002) 0.78
Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies. Recent Pat Biotechnol (2010) 0.78
Immunogenicity and protective effect against murine cerebral neosporosis of recombinant NcSRS2 in different iscom formulations. Vaccine (2007) 0.78
Staphylococcal display for combinatorial protein engineering of a head-to-tail affibody dimer binding the Alzheimer amyloid-β peptide. Biotechnol J (2012) 0.77
Affinity recovery of eight HER2-binding affibody variants using an anti-idiotypic affibody molecule as capture ligand. Protein Expr Purif (2010) 0.77
Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol. J Immunol Methods (2003) 0.77
Site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex. J Biotechnol (2004) 0.77
Fluorescence-activated cell sorting of specific affibody-displaying staphylococci. Appl Environ Microbiol (2003) 0.77
Achieving directed immunostimulating complexes incorporation. Expert Rev Vaccines (2006) 0.77
Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domain. N Biotechnol (2009) 0.76
Fluorescence-microscopy-based image analysis for analyte-dependent particle doublet detection in a single-step immunoagglutination assay. Anal Biochem (2005) 0.76
Order of amino acids in C-terminal cysteine-containing peptide-based chelators influences cellular processing and biodistribution of 99mTc-labeled recombinant Affibody molecules. Amino Acids (2011) 0.76
General expression vectors for Staphylococcus carnosus enabled efficient production of the outer membrane protein A of Klebsiella pneumoniae. FEMS Microbiol Lett (2002) 0.75
Strategy for highly selective ion-exchange capture using a charge-polarizing fusion partner. J Chromatogr A (2002) 0.75
Design and evaluation of radiolabeled tracers for tumor imaging. Biotechnol Appl Biochem (2013) 0.75
A high-stringency proteomics concept aimed for generation of antibodies specific for cDNA-encoded proteins. Biotechnol Appl Biochem (2002) 0.75
Single-vector three-frame expression systems for affinity-tagged proteins. FEMS Microbiol Lett (2002) 0.75
Affinity maturation of a TNFalpha-binding affibody molecule by Darwinian survival selection. Biotechnol Appl Biochem (2010) 0.75
Functional selection of phage displayed peptides for facilitated design of fusion tags improving aqueous two-phase partitioning of recombinant proteins. J Biotechnol (2002) 0.75
Affinity-based entrapment of the HER2 receptor in the endoplasmic reticulum using an affibody molecule. J Immunol Methods (2008) 0.75
Applying biotin-streptavidin binding for iscom (immunostimulating complex) association of recombinant immunogens. Biotechnol Appl Biochem (2005) 0.75