Published in Protein Eng on December 01, 2003
Evidence against a blood derived origin for transforming growth factor beta induced protein in corneal disorders caused by mutations in the TGFBI gene. Mol Vis (2007) 0.82
Optimizing the design of protein nanoparticles as carriers for vaccine applications. Nanomedicine (2015) 0.79
The albumin-binding domain as a scaffold for protein engineering. Comput Struct Biotechnol J (2013) 0.76
Towards a knowledge-based Human Protein Atlas. Nat Biotechnol (2010) 11.05
A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol Cell Proteomics (2005) 7.13
A genecentric Human Protein Atlas for expression profiles based on antibodies. Mol Cell Proteomics (2008) 6.45
Pyrosequencing: history, biochemistry and future. Clin Chim Acta (2005) 2.63
A global view of protein expression in human cells, tissues, and organs. Mol Syst Biol (2009) 2.41
Consequences of membrane protein overexpression in Escherichia coli. Mol Cell Proteomics (2007) 2.11
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling. Proteomics (2005) 1.87
The impact of tissue fixatives on morphology and antibody-based protein profiling in tissues and cells. J Histochem Cytochem (2009) 1.57
Anti-idiotypic protein domains selected from protein A-based affibody libraries. Proteins (2002) 1.52
Structural basis for recognition by an in vitro evolved affibody. Proc Natl Acad Sci U S A (2003) 1.46
A web-based tool for in silico biomarker discovery based on tissue-specific protein profiles in normal and cancer tissues. Mol Cell Proteomics (2007) 1.43
In vivo biotinylation and incorporation of a photo-inducible unnatural amino acid to an antibody-binding domain improve site-specific labeling of antibodies. Biotechnol J (2015) 1.39
An affibody in complex with a target protein: structure and coupled folding. Proc Natl Acad Sci U S A (2003) 1.35
The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD. Mol Biol Cell (2007) 1.33
Generation and validation of affinity reagents on a proteome-wide level. J Mol Recognit (2009) 1.29
Evolution of programmable zinc finger-recombinases with activity in human cells. J Mol Biol (2007) 1.28
Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP). J Proteome Res (2012) 1.11
High nuclear RBM3 expression is associated with an improved prognosis in colorectal cancer. Proteomics Clin Appl (2011) 1.10
Minimum information about a protein affinity reagent (MIAPAR). Nat Biotechnol (2010) 1.10
Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli. Biotechnol Appl Biochem (2002) 1.10
Microbead display of proteins by cell-free expression of anchored DNA. J Biotechnol (2003) 1.04
Engineering of a femtomolar affinity binding protein to human serum albumin. Protein Eng Des Sel (2008) 1.04
MAP20, a microtubule-associated protein in the secondary cell walls of hybrid aspen, is a target of the cellulose synthesis inhibitor 2,6-dichlorobenzonitrile. Plant Physiol (2008) 1.01
Enhancing the protein production levels in Escherichia coli with a strong promoter. FEBS J (2011) 1.00
Structure, specificity, and mode of interaction for bacterial albumin-binding modules. J Biol Chem (2001) 0.99
Single-step recovery and solid-phase refolding of inclusion body proteins using a polycationic purification tag. Biotechnol J (2006) 0.98
Mutational analysis of the interaction between albumin-binding domain from streptococcal protein G and human serum albumin. Protein Sci (2002) 0.97
Selective enrichment of monospecific polyclonal antibodies for antibody-based proteomics efforts. J Chromatogr A (2004) 0.96
A human protein atlas based on antibody proteomics. Curr Opin Mol Ther (2006) 0.95
Developments in biobanking workflow standardization providing sample integrity and stability. J Proteomics (2013) 0.94
Tissue profiling of the mammalian central nervous system using human antibody-based proteomics. Mol Cell Proteomics (2009) 0.94
Site-specifically conjugated anti-HER2 Affibody molecules as one-step reagents for target expression analyses on cells and xenograft samples. J Immunol Methods (2006) 0.93
From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies. Mol Cell Proteomics (2006) 0.92
A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein. J Biotechnol (2005) 0.92
Imaging of insulinlike growth factor type 1 receptor in prostate cancer xenografts using the affibody molecule 111In-DOTA-ZIGF1R:4551. J Nucl Med (2011) 0.91
High-throughput protein purification using an automated set-up for high-yield affinity chromatography. Protein Expr Purif (2006) 0.91
Evaluation of monospecific antibodies: a comparison study with commercial analogs using immunohistochemistry on tissue microarrays. Appl Immunohistochem Mol Morphol (2008) 0.91
A distal point mutation in the streptavidin-biotin complex preserves structure but diminishes binding affinity: experimental evidence of electronic polarization effects? Biochemistry (2010) 0.90
Development of affinity columns for the removal of high-abundance proteins in cerebrospinal fluid. Biotechnol Appl Biochem (2009) 0.90
Escherichia coli single-stranded DNA-binding protein, a molecular tool for improved sequence quality in pyrosequencing. Electrophoresis (2002) 0.90
A novel method for reproducible fluorescent labeling of small amounts of antibodies on solid phase. J Immunol Methods (2007) 0.89
High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week. Biotechnol J (2009) 0.88
A twisted four-sheeted model for an amyloid fibril. Structure (2005) 0.88
Multiplexed PrEST immunization for high-throughput affinity proteomics. J Immunol Methods (2006) 0.87
Covalent immunoglobulin labeling through a photoactivable synthetic Z domain. Bioconjug Chem (2011) 0.87
A small bispecific protein selected for orthogonal affinity purification. Biotechnol J (2010) 0.87
Integrated chromosome 19 transcriptomic and proteomic data sets derived from glioma cancer stem-cell lines. J Proteome Res (2013) 0.86
Selection of affibody molecules to the ligand-binding site of the insulin-like growth factor-1 receptor. Biotechnol Appl Biochem (2010) 0.86
Genome-based proteomics. Electrophoresis (2004) 0.86
Affibody molecules in protein capture microarrays: evaluation of multidomain ligands and different detection formats. J Proteome Res (2007) 0.85
Approaches for systematic proteome exploration. Biomol Eng (2007) 0.85
HAHAHA, HEHEHE, HIHIHI, or HKHKHK: influence of position and composition of histidine containing tags on biodistribution of [(99m)Tc(CO)3](+)-labeled affibody molecules. J Med Chem (2013) 0.85
Evaluation of a HER2-targeting affibody molecule combining an N-terminal HEHEHE-tag with a GGGC chelator for 99mTc-labelling at the C terminus. Tumour Biol (2012) 0.85
Parallel production and verification of protein products using a novel high-throughput screening method. Biotechnol J (2011) 0.84
Use of ENCODE resources to characterize novel proteoforms and missing proteins in the human proteome. J Proteome Res (2014) 0.83
Antibody performance in western blot applications is context-dependent. Biotechnol J (2014) 0.83
Engineering bispecificity into a single albumin-binding domain. PLoS One (2011) 0.83
Z(basic)--a novel purification tag for efficient protein recovery. J Chromatogr A (2007) 0.83
Improving the tolerance of a protein a analogue to repeated alkaline exposures using a bypass mutagenesis approach. Proteins (2004) 0.83
Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A. Eur J Biochem (2002) 0.83
[99mTc(CO)3]+-(HE)3-ZIGF1R:4551, a new Affibody conjugate for visualization of insulin-like growth factor-1 receptor expression in malignant tumours. Eur J Nucl Med Mol Imaging (2012) 0.82
Chromosome 19 annotations with disease speciation: a first report from the Global Research Consortium. J Proteome Res (2012) 0.82
Fluorescent detection of beta-lactamase activity in living Escherichia coli cells via esterase supplementation. FEMS Microbiol Lett (2005) 0.82
Association of chromosome 19 to lung cancer genotypes and phenotypes. Cancer Metastasis Rev (2015) 0.82
Second-contact shell mutation diminishes streptavidin-biotin binding affinity through transmitted effects on equilibrium dynamics. Biochemistry (2012) 0.82
High-throughput solubility assay for purified recombinant protein immunogens. Biochim Biophys Acta (2005) 0.81
Engineering streptococcal protein G for increased alkaline stability. Protein Eng (2002) 0.81
1H, 13C and 15N resonance assignments of an affibody-target complex. J Biomol NMR (2002) 0.81
Biophysical characterization of Z(SPA-1)--a phage-display selected binder to protein A. Protein Sci (2004) 0.81
An improved dual-expression concept, generating high-quality antibodies for proteomics research. Biotechnol Appl Biochem (2003) 0.80
Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samples. Biotechnol Appl Biochem (2010) 0.80
Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum). Protein Expr Purif (2003) 0.80
Selection and characterization of Affibody ligands to the transcription factor c-Jun. Biotechnol Appl Biochem (2009) 0.80
Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3. Cell Mol Life Sci (2013) 0.79
Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli. Nucleic Acids Res (2007) 0.79
Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology. J Biotechnol (2002) 0.79
HEHEHE-tagged affibody molecule may be purified by IMAC, is conveniently labeled with [⁹⁹(m)Tc(CO)₃](+), and shows improved biodistribution with reduced hepatic radioactivity accumulation. Bioconjug Chem (2010) 0.79
Affibody-mediated transferrin depletion for proteomics applications. Biotechnol J (2007) 0.79
Effect of substrate feed rate on recombinant protein secretion, degradation and inclusion body formation in Escherichia coli. Appl Microbiol Biotechnol (2005) 0.79
Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP. PLoS One (2013) 0.78
Investigating the applicability of antibodies generated within the human protein atlas as capture agents in immunoenrichment coupled to mass spectrometry. J Proteome Res (2014) 0.78
High-throughput protein purification under denaturating conditions by the use of cation exchange chromatography. Biotechnol J (2007) 0.78
Construction and characterization of affibody-Fc chimeras produced in Escherichia coli. J Immunol Methods (2002) 0.78
A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products. J Biotechnol (2002) 0.78
Miniaturized and automated high-throughput verification of proteins in the ISET platform with MALDI MS. Anal Chem (2012) 0.78
Evaluation of 99mTc-Z IGF1R:4551-GGGC affibody molecule, a new probe for imaging of insulin-like growth factor type 1 receptor expression. Amino Acids (2014) 0.77
Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol. J Immunol Methods (2003) 0.77
Inhibitory effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function. AMB Express (2014) 0.77
Selection and characterisation of affibody molecules inhibiting the interaction between Ras and Raf in vitro. N Biotechnol (2010) 0.77
Site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex. J Biotechnol (2004) 0.77
Affinity ligands for industrial protein purification. Protein Pept Lett (2005) 0.77
Microfluidic analysis of antibody specificity in a compact disk format. J Proteome Res (2006) 0.77
Orthogonal protein purification facilitated by a small bispecific affinity tag. J Vis Exp (2012) 0.77
Toward pyrosequencing on surface-attached genetic material by use of DNA-binding luciferase fusion proteins. Anal Biochem (2004) 0.76