Published in Biochem Biophys Res Commun on June 28, 2002
The genetic design of signaling cascades to record receptor activation. Proc Natl Acad Sci U S A (2007) 3.11
A combined approach to improving large-scale production of tobacco etch virus protease. Protein Expr Purif (2007) 2.62
Recombinant protein expression in Escherichia coli: advances and challenges. Front Microbiol (2014) 2.40
Inducible dimerization and inducible cleavage reveal a requirement for both processes in caspase-8 activation. J Biol Chem (2010) 1.99
The aggregation kinetics of Alzheimer's beta-amyloid peptide is controlled by stochastic nucleation. Protein Sci (2005) 1.74
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb Cell Fact (2005) 1.59
An overview of enzymatic reagents for the removal of affinity tags. Protein Expr Purif (2011) 1.46
Seneca Valley Virus 3Cpro Substrate Optimization Yields Efficient Substrates for Use in Peptide-Prodrug Therapy. PLoS One (2015) 1.42
Characterization of nucleocapsid binding by the measles virus and mumps virus phosphoproteins. J Virol (2004) 1.33
Retroviral nucleocapsid proteins display nonequivalent levels of nucleic acid chaperone activity. J Virol (2008) 1.24
Identification of luminal Loop 1 of Scap protein as the sterol sensor that maintains cholesterol homeostasis. J Biol Chem (2011) 1.18
Enhancing the stability and solubility of TEV protease using in silico design. Protein Sci (2007) 1.17
A novel method for high-level production of TEV protease by superfolder GFP tag. J Biomed Biotechnol (2010) 1.15
Self-cleavage of fusion protein in vivo using TEV protease to yield native protein. Protein Sci (2005) 1.13
Efficient protein depletion by genetically controlled deprotection of a dormant N-degron. Mol Syst Biol (2009) 1.12
An engineered second disulfide bond restricts lymphotactin/XCL1 to a chemokine-like conformation with XCR1 agonist activity. Biochemistry (2007) 1.11
The ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediated. PLoS One (2012) 1.08
Production of recombinant disulfide-rich venom peptides for structural and functional analysis via expression in the periplasm of E. coli. PLoS One (2013) 1.00
Distribution and biochemical properties of an M1-family aminopeptidase in Plasmodium falciparum indicate a role in vacuolar hemoglobin catabolism. J Biol Chem (2011) 0.99
C-terminal domain modulates the nucleic acid chaperone activity of human T-cell leukemia virus type 1 nucleocapsid protein via an electrostatic mechanism. J Biol Chem (2009) 0.99
An efficient method for the in vitro production of azol(in)e-based cyclic peptides. Angew Chem Int Ed Engl (2014) 0.99
Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease. J Lipid Res (2009) 0.99
The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal. Protein Expr Purif (2011) 0.96
High-throughput expression and purification of membrane proteins. J Struct Biol (2010) 0.93
Rapid modification of proteins using a rapamycin-inducible tobacco etch virus protease system. PLoS One (2009) 0.93
Biochemical characterization of Plasmodium falciparum dipeptidyl aminopeptidase 1. Mol Biochem Parasitol (2010) 0.89
Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem (2013) 0.88
Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteins. Adv Exp Med Biol (2012) 0.87
Seven novel modulators of the analgesic target NaV 1.7 uncovered using a high-throughput venom-based discovery approach. Br J Pharmacol (2015) 0.87
Expression, purification and structural characterization of functionally replete thrombospondin-1 type 1 repeats in a bacterial expression system. Protein Expr Purif (2011) 0.87
Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses. Protein Expr Purif (2008) 0.87
Evidence for catalytic roles for Plasmodium falciparum aminopeptidase P in the food vacuole and cytosol. J Biol Chem (2009) 0.87
Positional effects of fusion partners on the yield and solubility of MBP fusion proteins. Protein Expr Purif (2015) 0.86
A biobrick library for cloning custom eukaryotic plasmids. PLoS One (2011) 0.86
Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS Synth Biol (2014) 0.86
A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal influenza virus challenge. Hum Vaccin Immunother (2013) 0.86
Fusion partners can increase the expression of recombinant interleukins via transient transfection in 2936E cells. Protein Sci (2010) 0.85
Biochemical and biophysical characterization of recombinant rat apolipoprotein E: similarities to human apolipoprotein E3. Arch Biochem Biophys (2012) 0.85
The N-terminal peptide of mammalian GTP cyclohydrolase I is an autoinhibitory control element and contributes to binding the allosteric regulatory protein GFRP. J Biol Chem (2010) 0.85
Crystal structure and molecular modeling study of N-carbamoylsarcosine amidase Ta0454 from Thermoplasma acidophilum. J Struct Biol (2009) 0.84
Cooperativity to increase Turing pattern space for synthetic biology. ACS Synth Biol (2014) 0.83
High throughput quantitative expression screening and purification applied to recombinant disulfide-rich venom proteins produced in E. coli. J Vis Exp (2014) 0.83
An environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A. J Biol Chem (2011) 0.83
TEV protease-facilitated stoichiometric delivery of multiple genes using a single expression vector. Protein Sci (2010) 0.83
Novel fluorescence-assisted whole-cell assay for engineering and characterization of proteases and their substrates. Appl Environ Microbiol (2010) 0.82
Purification and Characterization of Recombinant N-Terminally Pyroglutamate-Modified Amyloid-β Variants and Structural Analysis by Solution NMR Spectroscopy. PLoS One (2015) 0.82
Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies. J Struct Funct Genomics (2015) 0.82
The pCri System: a vector collection for recombinant protein expression and purification. PLoS One (2014) 0.82
Differential temperature dependence of tobacco etch virus and rhinovirus 3C proteases. Anal Biochem (2013) 0.82
Enzyme activity in the crowded milieu. PLoS One (2012) 0.82
Structural and mechanistic studies of a stabilized subunit dimer variant of Escherichia coli bacterioferritin identify residues required for core formation. J Biol Chem (2009) 0.81
A yeast-based growth assay for the analysis of site-specific proteases. Nucleic Acids Res (2003) 0.81
A novel bioluminescent protease assay using engineered firefly luciferase. Curr Chem Genomics (2008) 0.80
Recombinant production of TEV cleaved human parathyroid hormone. J Pept Sci (2013) 0.80
Structural determinants of tobacco vein mottling virus protease substrate specificity. Protein Sci (2010) 0.80
Role of the ribosome-associated protein PY in the cold-shock response of Escherichia coli. Microbiologyopen (2013) 0.79
A tobacco etch virus protease with increased substrate tolerance at the P1' position. PLoS One (2013) 0.79
Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay. PLoS One (2011) 0.79
Structure of filamin A immunoglobulin-like repeat 10 from Homo sapiens. Acta Crystallogr Sect F Struct Biol Cryst Commun (2011) 0.78
Comparison of periplasmic and intracellular expression of Arabidopsis thionin proproteins in E. coli. Biotechnol Lett (2013) 0.78
Cloning, expression, crystallization and preliminary X-ray studies of a superfolder GFP fusion of cyanobacterial Psb32. Acta Crystallogr F Struct Biol Commun (2015) 0.78
Do-it-yourself histidine-tagged bovine enterokinase: a handy member of the protein engineer's toolbox. J Biotechnol (2013) 0.78
A cell-based protein-protein interaction method using a permuted luciferase reporter. Curr Chem Genomics (2011) 0.78
Recombinant production of the amino terminal cytoplasmic region of dengue virus non-structural protein 4A for structural studies. PLoS One (2014) 0.78
Linked production of pyroglutamate-modified proteins via self-cleavage of fusion tags with TEV protease and autonomous N-terminal cyclization with glutaminyl cyclase in vivo. PLoS One (2014) 0.78
A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol (2017) 0.78
Recombinant expression and purification of a tumor-targeted toxin in Bacillus anthracis. Biochem Biophys Res Commun (2012) 0.77
Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep). PLoS One (2013) 0.77
Synonymous rare arginine codons and tRNA abundance affect protein production and quality of TEV protease variant. PLoS One (2014) 0.77
Post-translational control of genetic circuits using Potyvirus proteases. Nucleic Acids Res (2016) 0.77
Direct Demonstration That Loop1 of Scap Binds to Loop7: A CRUCIAL EVENT IN CHOLESTEROL HOMEOSTASIS. J Biol Chem (2016) 0.77
Rapid large-scale purification of myofilament proteins using a cleavable His6-tag. Am J Physiol Heart Circ Physiol (2015) 0.77
Unrelated solubility-enhancing fusion partners MBP and NusA utilize a similar mode of action. Biotechnol Bioeng (2014) 0.76
Preparation of Specific Polyclonal Antibody Against the Recombinant Mutacin Produced by sfGFP Fusion Protein Technology. Open Microbiol J (2015) 0.76
Design of self-assembling peptide hydrogelators amenable to bacterial expression. Biomaterials (2014) 0.76
Engineering streptokinase for generation of active site-labeled plasminogen analogs. Anal Biochem (2011) 0.76
Crystallization and preliminary X-ray crystallographic analysis of the Bag2 amino-terminal domain from Mus musculus. Acta Crystallogr Sect F Struct Biol Cryst Commun (2012) 0.76
Expression, purification, crystallization and preliminary X-ray diffraction analysis of Bifidobacterium adolescentis xylose isomerase. Acta Crystallogr Sect F Struct Biol Cryst Commun (2013) 0.75
Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli. Microb Cell Fact (2017) 0.75
Chemoselective nitration of phenols with tert-butyl nitrite in solution and on solid support. Org Lett (2009) 0.75
Targeting protein function: the expanding toolkit for conditional disruption. Biochem J (2016) 0.75
Recombinant chymosin used for exact and complete removal of a prochymosin derived fusion tag releasing intact native target protein. Protein Sci (2009) 0.75
Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Mol Cell (2004) 6.08
Regulation of Raf-1 by direct feedback phosphorylation. Mol Cell (2005) 5.88
PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J Biol Chem (2007) 4.79
The parafibromin tumor suppressor protein is part of a human Paf1 complex. Mol Cell Biol (2005) 2.58
The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague. Structure (2004) 2.53
Alternative p38 activation pathway mediated by T cell receptor-proximal tyrosine kinases. Nat Immunol (2005) 2.31
Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III. Cell (2006) 2.15
Noncatalytic assembly of ribonuclease III with double-stranded RNA. Structure (2004) 2.04
Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli. Protein Sci (2005) 1.98
Expression and purification of soluble His(6)-tagged TEV protease. Methods Mol Biol (2009) 1.94
Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Protein Expr Purif (2004) 1.78
Structural basis for the substrate specificity of tobacco etch virus protease. J Biol Chem (2002) 1.72
CK2 Is a component of the KSR1 scaffold complex that contributes to Raf kinase activation. Curr Biol (2006) 1.69
Phosphorylation of HDM2 by Akt. Oncogene (2002) 1.63
Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners. Protein Expr Purif (2005) 1.49
A stepwise model for double-stranded RNA processing by ribonuclease III. Mol Microbiol (2007) 1.44
Maltose-binding protein as a solubility enhancer. Methods Mol Biol (2003) 1.40
Similar modes of polypeptide recognition by export chaperones in flagellar biosynthesis and type III secretion. Nat Struct Biol (2003) 1.39
Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. FEBS Lett (2003) 1.35
Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis. J Mol Biol (2005) 1.35
Serum insulin-like growth factor I: tumor marker or etiologic factor? A prospective study of prostate cancer among Finnish men. Cancer Res (2003) 1.31
Effect of sequence polymorphism and drug resistance on two HIV-1 Gag processing sites. Eur J Biochem (2002) 1.30
Selection and characterization of Yersinia pestis YopN mutants that constitutively block Yop secretion. Mol Microbiol (2005) 1.28
A generic method for the production of recombinant proteins in Escherichia coli using a dual hexahistidine-maltose-binding protein affinity tag. Methods Mol Biol (2007) 1.26
Structural characterization of the Yersinia pestis type III secretion system needle protein YscF in complex with its heterodimeric chaperone YscE/YscG. J Mol Biol (2008) 1.26
Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein. Acta Crystallogr D Biol Crystallogr (2002) 1.25
A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag. Nat Protoc (2007) 1.25
IL-6 enhances the nuclear translocation of DNA cytosine-5-methyltransferase 1 (DNMT1) via phosphorylation of the nuclear localization sequence by the AKT kinase. Cancer Genomics Proteomics (2008) 1.23
A spring-loaded state of NusG in its functional cycle is suggested by X-ray crystallography and supported by site-directed mutants. Biochemistry (2003) 1.22
Cell cycle-dependent phosphorylation of C/EBPbeta mediates oncogenic cooperativity between C/EBPbeta and H-RasV12. Mol Cell Biol (2004) 1.19
A pivotal role for reductive methylation in the de novo crystallization of a ternary complex composed of Yersinia pestis virulence factors YopN, SycN and YscB. Acta Crystallogr D Biol Crystallogr (2004) 1.16
Solution structure and functional analysis of the cysteine-rich C1 domain of kinase suppressor of Ras (KSR). J Mol Biol (2002) 1.14
High-resolution structure of the Yersinia pestis protein tyrosine phosphatase YopH in complex with a phosphotyrosyl mimetic-containing hexapeptide. Biochemistry (2003) 1.14
Structure of the Yersinia pestis type III secretion chaperone SycH in complex with a stable fragment of YscM2. Acta Crystallogr D Biol Crystallogr (2004) 1.13
Structure of ERA in complex with the 3' end of 16S rRNA: implications for ribosome biogenesis. Proc Natl Acad Sci U S A (2009) 1.11
Cellular inhibition of checkpoint kinase 2 (Chk2) and potentiation of camptothecins and radiation by the novel Chk2 inhibitor PV1019 [7-nitro-1H-indole-2-carboxylic acid {4-[1-(guanidinohydrazone)-ethyl]-phenyl}-amide]. J Pharmacol Exp Ther (2009) 1.11
Three-dimensional structure of the type III secretion chaperone SycE from Yersinia pestis. Acta Crystallogr D Biol Crystallogr (2002) 1.10
The ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediated. PLoS One (2012) 1.08
Structural basis for the function of stringent starvation protein a as a transcription factor. J Biol Chem (2005) 1.08
Atomic resolution structure of the cytoplasmic domain of Yersinia pestis YscU, a regulatory switch involved in type III secretion. Protein Sci (2009) 1.08
HIV-1 protease inhibitors: effects on HIV-2 replication and resistance. Trends Pharmacol Sci (2007) 1.06
RSK-mediated phosphorylation in the C/EBP{beta} leucine zipper regulates DNA binding, dimerization, and growth arrest activity. Mol Cell Biol (2010) 1.06
Intermediate states of ribonuclease III in complex with double-stranded RNA. Structure (2005) 1.03
Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins. J Struct Funct Genomics (2002) 1.03
Processive degradation of nascent polypeptides, triggered by tandem AGA codons, limits the accumulation of recombinant tobacco etch virus protease in Escherichia coli BL21(DE3). Protein Expr Purif (2002) 1.02
Functional analysis of C-TAK1 substrate binding and identification of PKP2 as a new C-TAK1 substrate. EMBO J (2003) 1.01
RNase III: Genetics and function; structure and mechanism. Annu Rev Genet (2013) 1.01
Effect of caspase cleavage-site phosphorylation on proteolysis. Biochem J (2003) 1.01
Mutations that alter the equilibrium between open and closed conformations of Escherichia coli maltose-binding protein impede its ability to enhance the solubility of passenger proteins. Biochem Biophys Res Commun (2007) 1.00
Regulation of CCAAT/enhancer-binding protein (C/EBP) activator proteins by heterodimerization with C/EBPgamma (Ig/EBP). J Biol Chem (2002) 1.00
Novel protein-protein interactions of the Yersinia pestis type III secretion system elucidated with a matrix analysis by surface plasmon resonance and mass spectrometry. J Biol Chem (2004) 0.99
Crystal structure of the Yersinia pestis GTPase activator YopE. Protein Sci (2002) 0.99
New protein fold revealed by a 1.65 A resolution crystal structure of Francisella tularensis pathogenicity island protein IglC. Protein Sci (2007) 0.98
Beta-lactam compounds as apparently uncompetitive inhibitors of HIV-1 protease. Bioorg Med Chem Lett (2005) 0.98
Critical role of novel Thr-219 autophosphorylation for the cellular function of PKCtheta in T lymphocytes. EMBO J (2005) 0.98
Overproduction, purification and structure determination of human dual-specificity phosphatase 14. Acta Crystallogr D Biol Crystallogr (2009) 0.97
Utilization of nitrophenylphosphates and oxime-based ligation for the development of nanomolar affinity inhibitors of the Yersinia pestis outer protein H (YopH) phosphatase. J Med Chem (2011) 0.95
Involvement of domain II in toxicity of anthrax lethal factor. J Biol Chem (2004) 0.94
Structure of the POZ domain of human LRF, a master regulator of oncogenesis. Biochem Biophys Res Commun (2006) 0.94
Crystal structure of the Yersinia type III secretion protein YscE. Protein Sci (2005) 0.93
Comparison of the substrate specificity of two potyvirus proteases. FEBS J (2005) 0.92
Narrow substrate specificity and sensitivity toward ligand-binding site mutations of human T-cell Leukemia virus type 1 protease. J Biol Chem (2004) 0.92
Structure-assisted discovery of Variola major H1 phosphatase inhibitors. Acta Crystallogr D Biol Crystallogr (2007) 0.92
Development of a microtiter plate fluorescent assay for inhibition studies on the HTLV-1 and HIV-1 proteinases. J Virol Methods (2004) 0.91
Crystal structure of checkpoint kinase 2 in complex with NSC 109555, a potent and selective inhibitor. Protein Sci (2009) 0.91
Isothiazolidinone (IZD) as a phosphoryl mimetic in inhibitors of the Yersinia pestis protein tyrosine phosphatase YopH. Acta Crystallogr D Biol Crystallogr (2011) 0.91
Amino acid preferences for a critical substrate binding subsite of retroviral proteases in type 1 cleavage sites. J Virol (2005) 0.91
Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli. Methods Mol Biol (2009) 0.90
Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner. Methods Mol Biol (2011) 0.90
Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri. Protein Sci (2008) 0.89
HIV-1 protease dimer interface mutations that compensate for viral reverse transcriptase instability in infectious virions. J Mol Biol (2007) 0.88
Structural basis for binding of RNA and cofactor by a KsgA methyltransferase. Structure (2009) 0.87
Structural biophysics of the NusB:NusE antitermination complex. J Mol Biol (2007) 0.87
Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses. Protein Expr Purif (2008) 0.87
The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA. Proc Natl Acad Sci U S A (2011) 0.87
Crystal structures of a high-affinity macrocyclic peptide mimetic in complex with the Grb2 SH2 domain. J Mol Biol (2005) 0.86
Direct photoaffinity labeling by dolastatin 10 of the amino-terminal peptide of beta-tubulin containing cysteine 12. J Biol Chem (2004) 0.86
Characterization of the murine leukemia virus protease and its comparison with the human immunodeficiency virus type 1 protease. J Gen Virol (2006) 0.86
Amino acid preferences of retroviral proteases for amino-terminal positions in a type 1 cleavage site. J Virol (2008) 0.86
A rapid oxime linker-based library approach to identification of bivalent inhibitors of the Yersinia pestis protein-tyrosine phosphatase, YopH. Bioorg Med Chem Lett (2010) 0.86
Structure and activity of Yersinia pestis 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase as a novel target for the development of antiplague therapeutics. Acta Crystallogr D Biol Crystallogr (2007) 0.85
Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3). Protein Expr Purif (2011) 0.85
Molecular modeling of nearly full-length ErbB2 receptor. Biophys J (2004) 0.85
Human cytolytic T cell recognition of Yersinia pestis virulence proteins that target innate immune responses. J Infect Dis (2006) 0.84
Structural characterization of inhibitor complexes with checkpoint kinase 2 (Chk2), a drug target for cancer therapy. J Struct Biol (2011) 0.84
Structure of human dual-specificity phosphatase 27 at 2.38 Å resolution. Acta Crystallogr D Biol Crystallogr (2011) 0.83
The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: insights into their use as tools for the removal of affinity tags. Protein Expr Purif (2010) 0.83
Overproduction, purification, and biochemical characterization of the dual specificity H1 protein phosphatase encoded by variola major virus. Protein Expr Purif (2006) 0.83
Differential temperature dependence of tobacco etch virus and rhinovirus 3C proteases. Anal Biochem (2013) 0.82
Human immunodeficiency virus type 1 capsid protein is a substrate of the retroviral proteinase while integrase is resistant toward proteolysis. Virology (2003) 0.81
Structure of the cytoplasmic domain of Yersinia pestis YscD, an essential component of the type III secretion system. Acta Crystallogr D Biol Crystallogr (2012) 0.81
Intracellular activation and deactivation of tasidotin, an analog of dolastatin 15: correlation with cytotoxicity. Mol Pharmacol (2008) 0.81
Therapeutic inhibition of pro-inflammatory signaling and toxicity to staphylococcal enterotoxin B by a synthetic dimeric BB-loop mimetic of MyD88. PLoS One (2012) 0.81