Published in FEBS Lett on December 18, 2002
Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Proc Natl Acad Sci U S A (2003) 12.91
Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization. EMBO J (2003) 1.48
Multiplexed and microparticle-based analyses: quantitative tools for the large-scale analysis of biological systems. Cytometry A (2006) 1.31
Nano-enabled synthetic biology. Mol Syst Biol (2007) 1.15
CIS display: In vitro selection of peptides from libraries of protein-DNA complexes. Proc Natl Acad Sci U S A (2004) 1.07
PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets. Nucleic Acids Res (2005) 1.07
Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization. Proc Natl Acad Sci U S A (2005) 1.02
Directed evolution of streptavidin variants using in vitro compartmentalization. Chem Biol (2008) 1.00
Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases. PLoS One (2010) 0.95
In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Protein Eng Des Sel (2013) 0.89
A high-throughput screen for antibiotic drug discovery. Biotechnol Bioeng (2013) 0.86
Evaluation of staphylococcal cell surface display and flow cytometry for postselectional characterization of affinity proteins in combinatorial protein engineering applications. Appl Environ Microbiol (2007) 0.86
Advances in the directed evolution of proteins. Curr Opin Chem Biol (2014) 0.85
Peptide aptamers: development and applications. Curr Top Med Chem (2015) 0.80
Conceptual and methodological advances in cell-free directed evolution. Curr Opin Struct Biol (2015) 0.77
Evolution of a mass spectrometry-grade protease with PTM-directed specificity. Proc Natl Acad Sci U S A (2016) 0.75
Chemoselective Coupling Preserves the Substrate Integrity of Surface-Immobilized Oligonucleotides for Emulsion PCR-Based Gene Library Construction. ACS Comb Sci (2016) 0.75
Expressible molecular colonies. Nucleic Acids Res (2005) 0.75
Kemp elimination catalysts by computational enzyme design. Nature (2008) 8.19
The 'evolvability' of promiscuous protein functions. Nat Genet (2004) 5.41
Antibody multispecificity mediated by conformational diversity. Science (2003) 5.21
Enzyme promiscuity: a mechanistic and evolutionary perspective. Annu Rev Biochem (2010) 4.41
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proc Natl Acad Sci U S A (2010) 3.72
Protein dynamism and evolvability. Science (2009) 3.57
Enzyme promiscuity: evolutionary and mechanistic aspects. Curr Opin Chem Biol (2006) 3.28
Conformational diversity and protein evolution--a 60-year-old hypothesis revisited. Trends Biochem Sci (2003) 3.24
Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein. Nature (2006) 3.19
Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. Lab Chip (2009) 3.01
Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms. Chem Biol (2008) 3.00
Drop-based microfluidic devices for encapsulation of single cells. Lab Chip (2008) 2.90
Amplification of complex gene libraries by emulsion PCR. Nat Methods (2006) 2.81
Stability effects of mutations and protein evolvability. Curr Opin Struct Biol (2009) 2.65
Chaperonin overexpression promotes genetic variation and enzyme evolution. Nature (2009) 2.61
Mutational effects and the evolution of new protein functions. Nat Rev Genet (2010) 2.60
Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes. Nat Struct Mol Biol (2004) 2.46
Multiplex picodroplet digital PCR to detect KRAS mutations in circulating DNA from the plasma of colorectal cancer patients. Clin Chem (2013) 2.45
The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters. Biochemistry (2011) 2.42
How protein stability and new functions trade off. PLoS Comput Biol (2008) 2.41
Single-cell analysis and sorting using droplet-based microfluidics. Nat Protoc (2013) 2.34
Quantitative and sensitive detection of rare mutations using droplet-based microfluidics. Lab Chip (2011) 2.26
The stability effects of protein mutations appear to be universally distributed. J Mol Biol (2007) 2.25
Intense neutral drifts yield robust and evolvable consensus proteins. J Mol Biol (2008) 2.11
The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase. Biochemistry (2006) 2.02
Initial mutations direct alternative pathways of protein evolution. PLoS Genet (2011) 2.01
Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization. Proc Natl Acad Sci U S A (2003) 1.83
Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase. Biochemistry (2005) 1.80
High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. Chem Biol (2005) 1.79
Latent evolutionary potentials under the neutral mutational drift of an enzyme. HFSP J (2007) 1.75
High-resolution dose-response screening using droplet-based microfluidics. Proc Natl Acad Sci U S A (2011) 1.69
Miniaturising the laboratory in emulsion droplets. Trends Biotechnol (2006) 1.67
Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis. Anal Chem (2009) 1.66
Advances in laboratory evolution of enzymes. Curr Opin Chem Biol (2008) 1.64
Do viral proteins possess unique biophysical features? Trends Biochem Sci (2008) 1.60
Evolved stereoselective hydrolases for broad-spectrum G-type nerve agent detoxification. Chem Biol (2012) 1.60
Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR. Lab Chip (2011) 1.58
Directed evolution of hydrolases for prevention of G-type nerve agent intoxication. Nat Chem Biol (2011) 1.57
High-throughput screens and selections of enzyme-encoding genes. Curr Opin Chem Biol (2005) 1.54
Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis. Nat Chem Biol (2012) 1.50
High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions. Chem Biol (2005) 1.48
Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization. EMBO J (2003) 1.48
Directed evolution by in vitro compartmentalization. Nat Methods (2006) 1.45
Directed evolution of proteins for heterologous expression and stability. Curr Opin Struct Biol (2005) 1.43
Evolutionary optimization of computationally designed enzymes: Kemp eliminases of the KE07 series. J Mol Biol (2009) 1.42
The specificity of cross-reactivity: promiscuous antibody binding involves specific hydrogen bonds rather than nonspecific hydrophobic stickiness. Protein Sci (2003) 1.38
The molecular basis of phosphate discrimination in arsenate-rich environments. Nature (2012) 1.38
Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59. Proc Natl Acad Sci U S A (2012) 1.36
Miniaturizing chemistry and biology in microdroplets. Chem Commun (Camb) (2007) 1.36
Directed enzyme evolution via small and effective neutral drift libraries. Nat Methods (2008) 1.35
Protein engineers turned evolutionists. Nat Methods (2007) 1.35
Potential role of phenotypic mutations in the evolution of protein expression and stability. Proc Natl Acad Sci U S A (2009) 1.35
A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution. Lab Chip (2012) 1.34
The 192R/Q polymorphs of serum paraoxonase PON1 differ in HDL binding, lipolactonase stimulation, and cholesterol efflux. J Lipid Res (2006) 1.34
Shared promiscuous activities and evolutionary features in various members of the amidohydrolase superfamily. Biochemistry (2005) 1.31
Structure and kinetics of a transient antibody binding intermediate reveal a kinetic discrimination mechanism in antigen recognition. Proc Natl Acad Sci U S A (2005) 1.29
Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries. Protein Eng Des Sel (2007) 1.29
Functional single-cell hybridoma screening using droplet-based microfluidics. Proc Natl Acad Sci U S A (2012) 1.29
The histidine 115-histidine 134 dyad mediates the lactonase activity of mammalian serum paraoxonases. J Biol Chem (2006) 1.28
Quantitative cell-based reporter gene assays using droplet-based microfluidics. Chem Biol (2010) 1.26
Divergence and convergence in enzyme evolution: parallel evolution of paraoxonases from quorum-quenching lactonases. J Biol Chem (2011) 1.24
Reliable microfluidic on-chip incubation of droplets in delay-lines. Lab Chip (2008) 1.23
Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution. J Mol Biol (2011) 1.22
Evolution of new protein topologies through multistep gene rearrangements. Nat Genet (2006) 1.22
High affinity, stability, and lactonase activity of serum paraoxonase PON1 anchored on HDL with ApoA-I. Biochemistry (2005) 1.21
Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nat Struct Mol Biol (2009) 1.21
In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting. Anal Biochem (2004) 1.20
Directed enzyme evolution: beyond the low-hanging fruit. Curr Opin Struct Biol (2012) 1.19
Reconstructing a missing link in the evolution of a recently diverged phosphotriesterase by active-site loop remodeling. Biochemistry (2012) 1.18
Noise-mean relationship in mutated promoters. Genome Res (2012) 1.18
Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme. Lab Chip (2009) 1.18
Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme. Nat Commun (2012) 1.16
What makes a protein fold amenable to functional innovation? Fold polarity and stability trade-offs. J Mol Biol (2013) 1.14
Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization. Protein Eng Des Sel (2004) 1.14
Ohno's model revisited: measuring the frequency of potentially adaptive mutations under various mutational drifts. Mol Biol Evol (2008) 1.13
Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase. FEBS J (2006) 1.12
Droplets as microreactors for high-throughput biology. Chembiochem (2007) 1.10
Catalytic versatility and backups in enzyme active sites: the case of serum paraoxonase 1. J Mol Biol (2012) 1.08
The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activity. J Lipid Res (2007) 1.08
In vivo administration of BL-3050: highly stable engineered PON1-HDL complexes. BMC Clin Pharmacol (2009) 1.08
Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation. Nucleic Acids Res (2002) 1.07
Kinetic aspects of emulsion stabilization by surfactants: a microfluidic analysis. Langmuir (2009) 1.06
A fast and efficient microfluidic system for highly selective one-to-one droplet fusion. Lab Chip (2009) 1.04
The evolutionary origins of detoxifying enzymes: the mammalian serum paraoxonases (PONs) relate to bacterial homoserine lactonases. J Biol Chem (2013) 1.04
Chromogenic and fluorogenic assays for the lactonase activity of serum paraoxonases. Chembiochem (2006) 1.04
Slow protein evolutionary rates are dictated by surface-core association. Proc Natl Acad Sci U S A (2011) 1.03
Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization. Proc Natl Acad Sci U S A (2005) 1.02
Reconstruction of functional beta-propeller lectins via homo-oligomeric assembly of shorter fragments. J Mol Biol (2006) 1.02
Avoiding and controlling double transformation artifacts. Protein Eng Des Sel (2007) 1.01
Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery. J Mol Biol (2004) 1.00
Analogues with fluorescent leaving groups for screening and selection of enzymes that efficiently hydrolyze organophosphorus nerve agents. J Med Chem (2006) 0.99
Protein insertions and deletions enabled by neutral roaming in sequence space. Mol Biol Evol (2013) 0.99
Role of chemistry versus substrate binding in recruiting promiscuous enzyme functions. Biochemistry (2011) 0.99