Oxidative stress in cell culture: an under-appreciated problem?

PubWeight™: 1.89‹?› | Rank: Top 3%

🔗 View Article (PMID 12681474)

Published in FEBS Lett on April 10, 2003

Authors

Barry Halliwell1

Author Affiliations

1: Department of Biochemistry, Faculty of Medicine, MD 7 #03-07, 8 Medical Drive, Singapore 117597, Singapore. bchbh@nus.edu.sg

Articles citing this

Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol (2004) 5.29

Vitamin E, antioxidant and nothing more. Free Radic Biol Med (2007) 3.87

Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Arch Toxicol (2013) 3.00

Regulation of hypoxia-inducible factor-1alpha by nitric oxide through mitochondria-dependent and -independent pathways. Biochem J (2003) 2.27

Disruption of murine Mus81 increases genomic instability and DNA damage sensitivity but does not promote tumorigenesis. Mol Cell Biol (2005) 2.00

Design and synthesis of compounds that extend yeast replicative lifespan. Aging Cell (2006) 1.93

The role of oxidative damage in the neuropathology of organic acidurias: insights from animal studies. J Inherit Metab Dis (2004) 1.75

Anaphase-promoting complex/cyclosome-Cdh1 coordinates glycolysis and glutaminolysis with transition to S phase in human T lymphocytes. Proc Natl Acad Sci U S A (2010) 1.50

Hyperpolarized [1-13C]-ascorbic and dehydroascorbic acid: vitamin C as a probe for imaging redox status in vivo. J Am Chem Soc (2011) 1.47

Characterization of transformed Arabidopsis with altered alternative oxidase levels and analysis of effects on reactive oxygen species in tissue. Plant Physiol (2005) 1.45

SENP3 is responsible for HIF-1 transactivation under mild oxidative stress via p300 de-SUMOylation. EMBO J (2009) 1.41

Quantification of basal and stimulated ROS levels as predictors of islet potency and function. Am J Transplant (2007) 1.24

SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress. J Biol Chem (2010) 1.16

c-Jun-deficient cells undergo premature senescence as a result of spontaneous DNA damage accumulation. Mol Cell Biol (2004) 1.13

Triterpenoid electrophiles (avicins) activate the innate stress response by redox regulation of a gene battery. J Clin Invest (2004) 1.12

Identification of regulatory pathways controlling gene expression of stress-responsive mitochondrial proteins in Arabidopsis. Plant Physiol (2008) 1.08

Citrinin induces apoptosis via a mitochondria-dependent pathway and inhibition of survival signals in embryonic stem cells, and causes developmental injury in blastocysts. Biochem J (2007) 1.06

The oxygen tension modulates acetaminophen-induced mitochondrial oxidant stress and cell injury in cultured hepatocytes. Toxicol Sci (2010) 1.05

The antioxidant paradox: less paradoxical now? Br J Clin Pharmacol (2013) 1.03

Oxidant stress-induced liver injury in vivo: role of apoptosis, oncotic necrosis, and c-Jun NH2-terminal kinase activation. Am J Physiol Gastrointest Liver Physiol (2009) 1.02

Ca2+-mediated mitochondrial reactive oxygen species metabolism augments Wnt/β-catenin pathway activation to facilitate cell differentiation. J Biol Chem (2014) 0.96

Wild-type p53-induced phosphatase 1 (Wip1) forestalls cellular premature senescence at physiological oxygen levels by regulating DNA damage response signaling during DNA replication. Cell Cycle (2014) 0.92

Epigallocatechin gallate (EGCG) potentiates the cytotoxicity of rotenone in neuroblastoma SH-SY5Y cells. Brain Res (2007) 0.92

Do polyphenols enter the brain and does it matter? Some theoretical and practical considerations. Genes Nutr (2011) 0.91

Composition of protein supplements used for human embryo culture. J Assist Reprod Genet (2014) 0.91

Glucose autoxidation induces functional damage to proteins via modification of critical arginine residues. Biochemistry (2011) 0.91

Redox modification of proteins as essential mediators of CNS autophagy and mitophagy. FEBS Lett (2013) 0.90

Rosmarinic acid-rich extracts of summer savory (Satureja hortensis L.) protect Jurkat T cells against oxidative stress. Oxid Med Cell Longev (2013) 0.89

Inhibition of citrinin-induced apoptotic biochemical signaling in human hepatoma G2 cells by resveratrol. Int J Mol Sci (2009) 0.89

Matrix metalloproteinase (MMP)-9 induced by Wnt signaling increases the proliferation and migration of embryonic neural stem cells at low O2 levels. J Biol Chem (2011) 0.88

Extracellular iron diminishes anticancer effects of vitamin C: an in vitro study. Sci Rep (2014) 0.88

Increased oxidative burden associated with traffic component of ambient particulate matter at roadside and urban background schools sites in London. PLoS One (2011) 0.87

Myths, artifacts, and fatal flaws: identifying limitations and opportunities in vitamin C research. Nutrients (2013) 0.86

Eye cancer: unique insights into oncogenesis: the Cogan Lecture. Invest Ophthalmol Vis Sci (2006) 0.86

Puromycin-based vectors promote a ROS-dependent recruitment of PML to nuclear inclusions enriched with HSP70 and Proteasomes. BMC Cell Biol (2009) 0.85

The reduction of reactive oxygen species formation by mitochondrial alternative respiration in tomato basal defense against TMV infection. Planta (2011) 0.85

PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples. Epigenetics Chromatin (2014) 0.84

Antioxidant protection from HIV-1 gp120-induced neuroglial toxicity. J Neuroinflammation (2004) 0.83

Murine adipose tissue-derived stromal cell apoptosis and susceptibility to oxidative stress in vitro are regulated by genetic background. PLoS One (2013) 0.83

Hsp27 is persistently expressed in zebrafish skeletal and cardiac muscle tissues but dispensable for their morphogenesis. Cell Stress Chaperones (2009) 0.82

Hazardous effects of curcumin on mouse embryonic development through a mitochondria-dependent apoptotic signaling pathway. Int J Mol Sci (2010) 0.82

Hop proanthocyanidins induce apoptosis, protein carbonylation, and cytoskeleton disorganization in human colorectal adenocarcinoma cells via reactive oxygen species. Food Chem Toxicol (2009) 0.81

Cell-autonomous progeroid changes in conditional mouse models for repair endonuclease XPG deficiency. PLoS Genet (2014) 0.81

Membrane Oxidation Enables the Cytosolic Entry of Polyarginine Cell-penetrating Peptides. J Biol Chem (2016) 0.80

Fluctuating vs. continuous exposure to H₂O₂: the effects on mitochondrial membrane potential, intracellular calcium, and NF-κB in astroglia. PLoS One (2013) 0.80

Alcohol: Friend or Foe? Alcoholic Beverage Hormesis for Cataract and Atherosclerosis is Related to Plasma Antioxidant Activity. Nonlinearity Biol Toxicol Med (2004) 0.79

An in vitro cell irradiation protocol for testing photopharmaceuticals and the effect of blue, green, and red light on human cancer cell lines. Photochem Photobiol Sci (2016) 0.79

Ochratoxin a inhibits mouse embryonic development by activating a mitochondrion-dependent apoptotic signaling pathway. Int J Mol Sci (2013) 0.79

The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes. Oxid Med Cell Longev (2015) 0.78

mRNA expression pattern of selected candidate genes differs in bovine oviductal epithelial cells in vitro compared with the in vivo state and during cell culture passages. Reprod Biol Endocrinol (2016) 0.78

Development of poly(ethylene glycol) hydrogels for salivary gland tissue engineering applications. Tissue Eng Part A (2015) 0.78

Transcriptome Profiling and Molecular Pathway Analysis of Genes in Association with Salinity Adaptation in Nile Tilapia Oreochromis niloticus. PLoS One (2015) 0.78

Cerebral epiphyseal proteins and melatonin modulate the hepatic and renal antioxidant defense of rats. Int J Nephrol (2011) 0.77

Disulfide scrambling in superoxide dismutase 1 reduces its cytotoxic effect in cultured cells and promotes protein aggregation. PLoS One (2013) 0.77

Oxidative stress under ambient and physiological oxygen tension in tissue culture. Curr Pharmacol Rep (2016) 0.77

The effects of reactive species on the tumorigenic phenotype of human head and neck squamous cell carcinoma (HNSCC) cells. Anticancer Res (2008) 0.77

Variability in APOE genotype status in human-derived cell lines: a cause for concern in cell culture studies? Genes Nutr (2013) 0.76

Are we over oxidized? Oxidative stress, cardiovascular disease, and the future of intervention studies with antioxidants. Vasc Health Risk Manag (2005) 0.76

Nuclear Factor κB1/RelA Mediates Inflammation in Human Lung Epithelial Cells at Atmospheric Oxygen Levels. J Cell Physiol (2015) 0.76

Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures. Mol Syst Biol (2017) 0.75

Differential responses of choroidal melanocytes and uveal melanoma cells to low oxygen conditions. Mol Vis (2017) 0.75

Peptide translocation through the plasma membrane of human cells: Can oxidative stress be exploited to gain better intracellular access? Commun Integr Biol (2016) 0.75

Electrochemical Potential Gradient as a Quantitative in Vitro Test Platform for Cellular Oxidative Stress. Antioxidants (Basel) (2016) 0.75

Spatial Characterization of Bioenergetics and Metabolism of Primordial to Preovulatory Follicles in Whole Ex Vivo Murine Ovary. Biol Reprod (2016) 0.75

Chromosomal disruption and rearrangements during murine sarcoma development converge to stable karyotypic formation kept by telomerase overexpression. J Biomed Sci (2016) 0.75

A brief review of in vitro models of diabetic neuropathy. Int J Diabetes Dev Ctries (2009) 0.75

A comparison of melatonin and α-lipoic acid in the induction of antioxidant defences in L6 rat skeletal muscle cells. Age (Dordr) (2015) 0.75

Influence of oxygen partial pressure on the characteristics of human hepatocarcinoma cells. Redox Biol (2017) 0.75

Hypoxia-regulated gene expression explains differences between melanoma cell line-derived xenografts and patient-derived xenografts. Oncotarget (2016) 0.75

Characterization and use of HapT1-derived homologous tumors as a preclinical model to evaluate therapeutic efficacy of drugs against pancreatic tumor desmoplasia. Oncotarget (2016) 0.75

Withaferin A protects against palmitic acid-induced endothelial insulin resistance and dysfunction through suppression of oxidative stress and inflammation. Sci Rep (2016) 0.75

Cellular senescence, senescence-associated secretory phenotype, and chronic kidney disease. Oncotarget (2017) 0.75

PPAR-delta promotes survival of chronic lymphocytic leukemia cells in energetically unfavorable conditions. Leukemia (2017) 0.75

Distinct radiation responses after in vitro mtDNA depletion are potentially related to oxidative stress. PLoS One (2017) 0.75

Ascorbic acid alters cell fate commitment of human neural progenitors in a WNT/β-catenin/ROS signaling dependent manner. J Biomed Sci (2017) 0.75